Mercurial > repos > greg > vsnp_add_zero_coverage
diff vsnp_add_zero_coverage.xml @ 2:01312f8a6ca9 draft
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author | greg |
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date | Sun, 03 Jan 2021 16:29:00 +0000 |
parents | 3cb0bf7e1b2d |
children | bb6cc994707d |
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--- a/vsnp_add_zero_coverage.xml Tue Apr 21 09:51:00 2020 -0400 +++ b/vsnp_add_zero_coverage.xml Sun Jan 03 16:29:00 2021 +0000 @@ -1,147 +1,72 @@ -<tool id="vsnp_add_zero_coverage" name="vSNP: add zero coverage" version="1.0.0"> +<tool id="vsnp_add_zero_coverage" name="vSNP: add zero coverage" version="@WRAPPER_VERSION@.2" profile="@PROFILE@"> <description></description> + <macros> + <import>macros.xml</import> + </macros> <requirements> <requirement type="package" version="1.76">biopython</requirement> - <requirement type="package" version="1.16.5">numpy</requirement> <requirement type="package" version="0.25.3">pandas</requirement> <requirement type="package" version="0.15.4">pysam</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ -#import os #import re -#set input_type = $input_type_cond.input_type -#set input_bam_dir = 'input_bam_dir' -#set input_vcf_dir = 'input_vcf_dir' -#set output_vcf_dir = 'output_vcf_dir' -#set output_metrics_dir = 'output_metrics_dir' -mkdir -p $input_bam_dir && -mkdir -p $input_vcf_dir && -mkdir -p $output_vcf_dir && -mkdir -p $output_metrics_dir && -#if str($input_type) == "single": - #set bam_input = $input_type_cond.bam_input - #set file_name = $bam_input.file_name - #set file_name_base = $os.path.basename($file_name) - ln -s $file_name $input_bam_dir/$file_name_base && - #set vcf_input = $input_type_cond.vcf_input - #set file_name = $vcf_input.file_name - #set file_name_base = $os.path.basename($file_name) - ln -s $file_name $input_vcf_dir/$file_name_base && -#else: - #for $i in $input_type_cond.bam_input_collection: - #set filename = $i.file_name - #set identifier = re.sub('[^\s\w\-]', '_', str($i.element_identifier)) - ln -s $filename $input_bam_dir/$identifier && - #end for - #for $i in $input_type_cond.vcf_input_collection: - #set filename = $i.file_name - #set identifier = re.sub('[^\s\w\-]', '_', str($i.element_identifier)) - ln -s $filename $input_vcf_dir/$identifier && - #end for -#end if + +## The identifer for both of the following files is likely the same +## string, so we append a file extension to allow for both links. +#set bam_identifier = re.sub('[^\s\w\-]', '_', str($bam_input.element_identifier)) + '.bam' +ln -s '${bam_input}' '${bam_identifier}' && +#set vcf_identifier = re.sub('[^\s\w\-]', '_', str($vcf_input.element_identifier)) + '.vcf' +ln -s '${vcf_input}' '${vcf_identifier}' && + python '$__tool_directory__/vsnp_add_zero_coverage.py' ---processes $processes -#if str($reference_cond.reference_source) == "cached" +--bam_input '$bam_identifier' +--vcf_input '$vcf_identifier' +#if str($reference_cond.reference_source) == 'cached' --reference '$reference_cond.reference.fields.path' #else: --reference '$reference_cond.reference' #end if -#if str($input_type) == "single": - --output_metrics '$output_metrics' - --output_vcf '$output_vcf' -#end if +--output_metrics '$output_metrics' +--output_vcf '$output_vcf' ]]></command> <inputs> - <conditional name="input_type_cond"> - <param name="input_type" type="select" label="Choose the category of the files to be analyzed"> - <option value="single" selected="true">Single files</option> - <option value="collection">Collections of files</option> - </param> - <when value="single"> - <param name="bam_input" type="data" format="bam" label="BAM file"> - <validator type="unspecified_build"/> - </param> - <param name="vcf_input" type="data" format="vcf" label="VCF file"> - <validator type="unspecified_build"/> - </param> - </when> - <when value="collection"> - <param name="bam_input_collection" type="data_collection" format="bam" collection_type="list" label="Collection of BAM files"> - <validator type="unspecified_build"/> - </param> - <param name="vcf_input_collection" type="data_collection" format="vcf" collection_type="list" label="Collection of VCF files"> - <validator type="unspecified_build"/> - </param> - </when> - </conditional> + <param name="bam_input" type="data" format="bam" label="BAM file"/> + <param name="vcf_input" type="data" format="vcf" label="VCF file"/> <conditional name="reference_cond"> - <param name="reference_source" type="select" label="Choose the source for the reference genome"> - <option value="cached" selected="true">locally cached</option> - <option value="history">from history</option> - </param> + <expand macro="param_reference_source"/> <when value="cached"> <param name="reference" type="select" label="Using reference genome"> - <options from_data_table="fasta_indexes"/> - <!-- No <filter> tag here! --> - <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected BAM file"/> + <options from_data_table="fasta_indexes"> + <filter type="data_meta" column="1" key="dbkey" ref="bam_input"/> + <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected BAM file"/> + </options> </param> </when> <when value="history"> - <param name="reference" type="data" format="fasta" label="Using reference genome"> + <param name="reference" type="data" format="fasta,fasta.gz" label="Using reference genome"> <validator type="no_options" message="The current history does not include a fasta dataset"/> </param> </when> </conditional> - <param name="processes" type="integer" min="1" max="20" value="8" label="Number of processes for job splitting"/> </inputs> <outputs> - <data name="output_vcf" format="vcf" label="${tool.name} (filtered VCF) on ${on_string}"> - <filter>input_type_cond['input_type'] == 'single'</filter> - </data> - <collection name="output_vcf_collection" type="list" label="${tool.name} (filtered VCFs) on ${on_string}"> - <discover_datasets pattern="__name__" directory="output_vcf_dir" format="vcf" /> - <filter>input_type_cond['input_type'] == 'collection'</filter> - </collection> - <data name="output_metrics" format="tabular" label="${tool.name} (metrics) on ${on_string}"> - <filter>input_type_cond['input_type'] == 'single'</filter> - </data> - <collection name="output_metrics_collection" type="list" label="${tool.name} (metrics) on ${on_string}"> - <discover_datasets pattern="__name__" directory="output_metrics_dir" format="tabular" /> - <filter>input_type_cond['input_type'] == 'collection'</filter> - </collection> + <data name="output_vcf" format="vcf" label="${tool.name} on ${on_string} (filtered VCF)"/> + <data name="output_metrics" format="tabular" label="${tool.name} on ${on_string} (metrics)"/> </outputs> <tests> - <test> - <param name="input_type" value="collection"/> - <param name="bam_input_collection"> - <collection type="list"> - <element name="bam_input.bam" value="bam_input.bam" dbkey="89"/> - <element name="bam_input2.bam" value="bam_input2.bam" dbkey="89"/> - </collection> - </param> - <param name="vcf_input_collection"> - <collection type="list"> - <element name="vcf_input.vcf" value="vcf_input.vcf" dbkey="89"/> - <element name="vcf_input2.vcf" value="vcf_input2.vcf" dbkey="89"/> - </collection> - </param> - <param name="reference_source" value="history"/> - <param name="reference" value="NC_002945v4.fasta" ftype="fasta"/> - <output_collection name="output_vcf_collection" type="list"> - <element name="vcf_input.vcf" file="output_vcf.vcf" ftype="vcf" compare="contains"/> - <element name="vcf_input2.vcf" file="output_vcf.vcf" ftype="vcf" compare="contains"/> - </output_collection> - <output_collection name="output_metrics_collection" type="list"> - <element name="vcf_input.tabular" file="output_metrics.tabular" ftype="tabular" compare="contains"/> - <element name="vcf_input2.tabular" file="output_metrics.tabular" ftype="tabular" compare="contains"/> - </output_collection> - </test> - <test> + <test expect_num_outputs="2"> <param name="bam_input" value="bam_input.bam" ftype="bam" dbkey="89"/> <param name="vcf_input" value="vcf_input.vcf" ftype="vcf" dbkey="89"/> <param name="reference_source" value="history"/> <param name="reference" value="NC_002945v4.fasta" ftype="fasta"/> - <param name="output_vcf" value="output_vcf.vcf" ftype="vcf" compare="contains"/> + <output name="output_vcf" value="output_vcf.vcf" ftype="vcf" compare="contains"/> + <output name="output_metrics" file="output_metrics.tabular" ftype="tabular"/> + </test> + <test expect_num_outputs="2"> + <param name="bam_input" value="bam_input.bam" ftype="bam" dbkey="89"/> + <param name="vcf_input" value="vcf_input.vcf" ftype="vcf" dbkey="89"/> + <param name="reference_source" value="cached"/> + <output name="output_vcf" value="output_vcf.vcf" ftype="vcf" compare="contains"/> <output name="output_metrics" file="output_metrics.tabular" ftype="tabular" compare="contains"/> </test> </tests> @@ -157,19 +82,8 @@ **Required Options** - * **Choose the category of the files to be analyzed** - select "Single files" or "Collections of files", then select the appropriate history items (single BAM and VCF files or collections of BAM and VCF files) based on the selected option. * **Choose the source for the reference genome** - select "locally cached" if the reference associated with the BAM and VCF files is available within the Galaxy environment or "from history" to select the reference from the current history. - * **Number of processes for job splitting** - Select the number of processes for splitting the job to shorten execution time. </help> - <citations> - <citation type="bibtex"> - @misc{None, - journal = {None}, - author = {1. Stuber T}, - title = {Manuscript in preparation}, - year = {None}, - url = {https://github.com/USDA-VS/vSNP},} - </citation> - </citations> + <expand macro="citations" /> </tool>