# HG changeset patch # User greg # Date 1609688848 0 # Node ID 2d6c6b01319efe3ca865352d10f91a6eea61b567 # Parent 321a8259e3f9225fc9c7d87bdf25d0d22c8d3f7d Uploaded diff -r 321a8259e3f9 -r 2d6c6b01319e macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Sun Jan 03 15:47:28 2021 +0000 @@ -0,0 +1,24 @@ + + + 1.0 + 19.09 + + + + + + + + + + @misc{None, + journal = {None}, + author = {1. Stuber T}, + title = {Manuscript in preparation}, + year = {None}, + url = {https://github.com/USDA-VS/vSNP},} + + + + + diff -r 321a8259e3f9 -r 2d6c6b01319e vsnp_statistics.py --- a/vsnp_statistics.py Thu Apr 30 11:01:40 2020 -0400 +++ b/vsnp_statistics.py Sun Jan 03 15:47:28 2021 +0000 @@ -2,14 +2,12 @@ import argparse import gzip -import numpy import os -import pandas import shutil -INPUT_IDXSTATS_DIR = 'input_idxstats' -INPUT_METRICS_DIR = 'input_metrics' -INPUT_READS_DIR = 'input_reads' +import numpy +import pandas + QUALITYKEY = {'!': '0', '"': '1', '#': '2', '$': '3', '%': '4', '&': '5', "'": '6', '(': '7', ')': '8', '*': '9', '+': '10', ',': '11', '-': '12', '.': '13', '/': '14', '0': '15', '1': '16', '2': '17', '3': '18', '4': '19', '5': '20', '6': '21', '7': '22', @@ -26,24 +24,9 @@ def fastq_to_df(fastq_file, gzipped): - if gzipped.lower() == "true": + if gzipped: return pandas.read_csv(gzip.open(fastq_file, "r"), header=None, sep="^") - else: - return pandas.read_csv(open(fastq_file, "r"), header=None, sep="^") - - -def get_base_file_name(file_path): - base_file_name = os.path.basename(file_path) - if base_file_name.find(".") > 0: - # Eliminate the extension. - return os.path.splitext(base_file_name)[0] - elif base_file_name.find("_") > 0: - # The dot extension was likely changed to - # the " character. - items = base_file_name.split("_") - return "_".join(items[0:-1]) - else: - return base_file_name + return pandas.read_csv(open(fastq_file, "r"), header=None, sep="^") def nice_size(size): @@ -67,14 +50,14 @@ return '??? bytes' -def output_statistics(reads_files, idxstats_files, metrics_files, output_file, gzipped, dbkey): +def output_statistics(fastq_files, idxstats_files, metrics_files, output_file, gzipped, dbkey): # Produce an Excel spreadsheet that # contains a row for each sample. columns = ['Reference', 'File Size', 'Mean Read Length', 'Mean Read Quality', 'Reads Passing Q30', 'Total Reads', 'All Mapped Reads', 'Unmapped Reads', 'Unmapped Reads Percentage of Total', 'Reference with Coverage', 'Average Depth of Coverage', 'Good SNP Count'] data_frames = [] - for i, fastq_file in enumerate(reads_files): + for i, fastq_file in enumerate(fastq_files): idxstats_file = idxstats_files[i] metrics_file = metrics_files[i] file_name_base = os.path.basename(fastq_file) @@ -171,44 +154,48 @@ return ref_with_coverage, avg_depth_of_coverage, good_snp_count -if __name__ == '__main__': - parser = argparse.ArgumentParser() +parser = argparse.ArgumentParser() - parser.add_argument('--read1', action='store', dest='read1', required=False, default=None, help='Required: single read') - parser.add_argument('--read2', action='store', dest='read2', required=False, default=None, help='Optional: paired read') - parser.add_argument('--dbkey', action='store', dest='dbkey', help='Reference dbkey') - parser.add_argument('--gzipped', action='store', dest='gzipped', help='Input files are gzipped') - parser.add_argument('--samtools_idxstats', action='store', dest='samtools_idxstats', required=False, default=None, help='Output of samtools_idxstats') - parser.add_argument('--output', action='store', dest='output', help='Output Excel statistics file') - parser.add_argument('--vsnp_azc', action='store', dest='vsnp_azc', required=False, default=None, help='Output of vsnp_add_zero_coverage') +parser.add_argument('--dbkey', action='store', dest='dbkey', help='Reference dbkey') +parser.add_argument('--gzipped', action='store_true', dest='gzipped', required=False, default=False, help='Input files are gzipped') +parser.add_argument('--input_idxstats_dir', action='store', dest='input_idxstats_dir', required=False, default=None, help='Samtools idxstats input directory') +parser.add_argument('--input_metrics_dir', action='store', dest='input_metrics_dir', required=False, default=None, help='vSNP add zero coverage metrics input directory') +parser.add_argument('--input_reads_dir', action='store', dest='input_reads_dir', required=False, default=None, help='Samples input directory') +parser.add_argument('--list_paired', action='store_true', dest='list_paired', required=False, default=False, help='Input samples is a list of paired reads') +parser.add_argument('--output', action='store', dest='output', help='Output Excel statistics file') +parser.add_argument('--read1', action='store', dest='read1', help='Required: single read') +parser.add_argument('--read2', action='store', dest='read2', required=False, default=None, help='Optional: paired read') +parser.add_argument('--samtools_idxstats', action='store', dest='samtools_idxstats', help='Output of samtools_idxstats') +parser.add_argument('--vsnp_azc', action='store', dest='vsnp_azc', help='Output of vsnp_add_zero_coverage') - args = parser.parse_args() - print("args:\n%s\n" % str(args)) +args = parser.parse_args() - reads_files = [] - idxstats_files = [] - metrics_files = [] - # Accumulate inputs. - if args.read1 is not None: - # The inputs are not dataset collections, so - # read1, read2 (possibly) and vsnp_azc will also - # not be None. - reads_files.append(args.read1) +fastq_files = [] +idxstats_files = [] +metrics_files = [] +# Accumulate inputs. +if args.read1 is not None: + # The inputs are not dataset collections, so + # read1, read2 (possibly) and vsnp_azc will also + # not be None. + fastq_files.append(args.read1) + idxstats_files.append(args.samtools_idxstats) + metrics_files.append(args.vsnp_azc) + if args.read2 is not None: + fastq_files.append(args.read2) idxstats_files.append(args.samtools_idxstats) metrics_files.append(args.vsnp_azc) - if args.read2 is not None: - reads_files.append(args.read2) - idxstats_files.append(args.samtools_idxstats) - metrics_files.append(args.vsnp_azc) - else: - for file_name in sorted(os.listdir(INPUT_READS_DIR)): - file_path = os.path.abspath(os.path.join(INPUT_READS_DIR, file_name)) - reads_files.append(file_path) - base_file_name = get_base_file_name(file_path) - for file_name in sorted(os.listdir(INPUT_IDXSTATS_DIR)): - file_path = os.path.abspath(os.path.join(INPUT_IDXSTATS_DIR, file_name)) - idxstats_files.append(file_path) - for file_name in sorted(os.listdir(INPUT_METRICS_DIR)): - file_path = os.path.abspath(os.path.join(INPUT_METRICS_DIR, file_name)) - metrics_files.append(file_path) - output_statistics(reads_files, idxstats_files, metrics_files, args.output, args.gzipped, args.dbkey) +else: + for file_name in sorted(os.listdir(args.input_reads_dir)): + fastq_files.append(os.path.join(args.input_reads_dir, file_name)) + for file_name in sorted(os.listdir(args.input_idxstats_dir)): + idxstats_files.append(os.path.join(args.input_idxstats_dir, file_name)) + if args.list_paired: + # Add the idxstats file for reverse. + idxstats_files.append(os.path.join(args.input_idxstats_dir, file_name)) + for file_name in sorted(os.listdir(args.input_metrics_dir)): + metrics_files.append(os.path.join(args.input_metrics_dir, file_name)) + if args.list_paired: + # Add the metrics file for reverse. + metrics_files.append(os.path.join(args.input_metrics_dir, file_name)) +output_statistics(fastq_files, idxstats_files, metrics_files, args.output, args.gzipped, args.dbkey) diff -r 321a8259e3f9 -r 2d6c6b01319e vsnp_statistics.xml --- a/vsnp_statistics.xml Thu Apr 30 11:01:40 2020 -0400 +++ b/vsnp_statistics.xml Sun Jan 03 15:47:28 2021 +0000 @@ -1,5 +1,8 @@ - + + + macros.xml + numpy pandas @@ -7,102 +10,113 @@ xlsxwriter - - + + - + - - + + - + + + + + + + + + + + + + + - + + + + - - - - - - - - - - - + + @@ -110,36 +124,81 @@ - + + + + + + + + + + + + + - - - + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + **What it does** -Accepts a single fastqsanger sample, a set of paired read samples, or a collections of samples along with associated -SAMtools idxstats and vSNP zero coverage metrics files and extracts information from them to produce an Excel -spreadsheet containing statistics for each sample. Statistics include reference, file size, mean read length, mean -read quality, reads passing Q30, total reads, all mapped reads, unmapped reads, unmapped reads percentage of total, -reference with coverage, average depth of coverage and good SNP count. - -**Required options** - - * **Choose the type for files to be analyzed** - select "Single files" or "Collections of files", then select the appropriate history items (single or paired fastqsanger reads or collections of fastqsanger reads and associated idxstats and vSNP zero coverage metrics files) based on the selected option.. +Accepts associated fastq files, SAMtools idxstats files and **vSNP: add zero coverage** metrics files and extracts information from them +to produce an Excel spreadsheet containing statistics for each sample. The samples can be single or paired reads, and all associated inputs +can be either single files or collections of files. The output statistics include reference, file size, mean read length, mean read quality, +reads passing Q30, total reads, all mapped reads, unmapped reads, unmapped reads percentage of total, reference with coverage, average depth +of coverage and good SNP count. - - - @misc{None, - journal = {None}, - author = {1. Stuber T}, - title = {Manuscript in preparation}, - year = {None}, - url = {https://github.com/USDA-VS/vSNP},} - - +