Mercurial > repos > hepcat72 > extractsplitreads_bwamem
comparison extractSplitReads_BwaMem.xml @ 0:839c219ab0aa draft
planemo upload commit 0066847e0ac19632c3091ac3148069de3bb3855f-dirty
author | hepcat72 |
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date | Fri, 10 Aug 2018 11:46:23 -0400 |
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children | a20ac5d90c16 |
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1 <tool id="extractsplitreads_bwamem" | |
2 name="Extract Split Reads" | |
3 version="0.1.0"> | |
4 | |
5 <description>from bwaMEM (part of lumpy-sv)</description> | |
6 | |
7 <requirements> | |
8 <requirement type="package" version="0.2.14a">lumpy-sv</requirement> | |
9 </requirements> | |
10 | |
11 <!-- Not sure yet how to call the command if the lumpy-sv package in bioconda doesn't put the script in an executable location on PATH --> | |
12 | |
13 <version_command>extractSplitReads_BwaMem | grep ^extractSplitReads_BwaMem | cut -d v -f 2</version_command> | |
14 | |
15 <command detect_errors="aggressive"> | |
16 <![CDATA[ | |
17 extractSplitReads_BwaMem | |
18 -i '$input' | |
19 -n $numSplits | |
20 $includeDups | |
21 -m $minNonOverlap | |
22 > '$output' | |
23 ]]> | |
24 </command> | |
25 | |
26 <inputs> | |
27 | |
28 <param format="sam" name="input" type="data" label="Sam alignment file" argument="--infile"/> | |
29 | |
30 <param name="numSplits" | |
31 label="numSplits" | |
32 argument="--numSplits" | |
33 | |
34 type="integer" | |
35 value="2" | |
36 | |
37 help="The maximum number of split-read mappings to allow per read. Reads with more are excluded." /> | |
38 | |
39 <param name="includeDups" | |
40 label="includeDups" | |
41 argument="--includeDups" | |
42 | |
43 type="boolean" | |
44 truevalue="--includeDups" | |
45 falsevalue="" | |
46 checked="no" | |
47 value="false" | |
48 | |
49 help="Include alignments marked as duplicates." /> | |
50 | |
51 <param name="minNonOverlap" | |
52 label="minNonOverlap" | |
53 argument="--minNonOverlap" | |
54 | |
55 type="integer" | |
56 value="20" | |
57 | |
58 help="Minimum non-overlap between split alignments on the query." /> | |
59 | |
60 </inputs> | |
61 <outputs> | |
62 <data format="sam" name="output" /> | |
63 </outputs> | |
64 | |
65 <tests> | |
66 <test> | |
67 <param name="input" value="JS01-BWA-MEM.splitters.sam"/> | |
68 <output name="output" file="JS01-BWA-MEM.splitters.out.sam"/> | |
69 </test> | |
70 <test> | |
71 <param name="input" value="pe.pos_sorted.sam"/> | |
72 <output name="output" file="pe.pos_sorted.out.sam"/> | |
73 </test> | |
74 </tests> | |
75 | |
76 <help> | |
77 <![CDATA[ | |
78 | |
79 Usage: `extractSplitReads_BwaMem -i <file>` | |
80 | |
81 extractSplitReads_BwaMem v0.1.0 | |
82 | |
83 Author: Ira Hall | |
84 | |
85 Description: Get split-read alignments from bwa-mem in lumpy compatible format. Ignores reads marked as duplicates. | |
86 | |
87 Works on read or position sorted SAM input. Tested on bwa mem v0.7.5a-r405. | |
88 | |
89 | |
90 Options: | |
91 -h, --help show this help message and exit | |
92 -i FILE, --inFile=FILE | |
93 A SAM file or standard input (-i stdin). | |
94 -n INT, --numSplits=INT | |
95 The maximum number of split-read mappings to allow per | |
96 read. Reads with more are excluded. Default=2 | |
97 -d, --includeDups Include alignments marked as duplicates. Default=False | |
98 -m INT, --minNonOverlap=INT | |
99 minimum non-overlap between split alignments on the | |
100 query (default=20) | |
101 | |
102 ]]> | |
103 </help> | |
104 | |
105 <citations> | |
106 <citation type="doi">doi:10.1186/gb-2014-15-6-r84</citation> | |
107 </citations> | |
108 | |
109 </tool> |