# HG changeset patch # User hyungrolee # Date 1455525199 18000 # Node ID 7658ef159fd80ff9cc8a13f2c64f8626d69787bc # Parent f9be9a13e01a0f959e2e6af93fb27053d31475d8 Uploaded diff -r f9be9a13e01a -r 7658ef159fd8 mgescan.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/mgescan.xml Mon Feb 15 03:33:19 2016 -0500 @@ -0,0 +1,123 @@ + + + + + MGEScan + + + mgescan + + mgescan --version + + mgescan.sh $input '$input.name' 3 $output $program $clade $qvalue_en $qvalue_rt $ltr_gff3 $nonltr_gff3 $both_gff3 $mpi_yn.nmpi + + + + + + + + + + + + + + + + + + + + + + + + + + program != "N" + + + program != "L" + + + program != "L" + + + program != "L" + + + program != "N" + + + program != "L" + + + program == "B" + + + + +How to Run MGEScan +=================== + +* Select an input genome data from the select box, and choose a program. Both LTR and nonLTR of MGEScan is default. +* Click 'Execute' button. +* MPI will be enabled depending on your system support. + +If you like to have more options to run LTR or nonLTR program, use separated tools on the left panel. + +For example, in LTR > MGEScan-LTR, preprocessing by repeatmasker and setting other variables are available e.g. distance(bp) between LTRs. + +Output +============ + +A. MGEScan_LTR: + +Upon completion, MGEScan-LTR generates a file "ltr.out". This output file has information +about clusters and coordinates of LTR retrotransposons identified. Each cluster of LTR +retrotransposons starts with the head line of "[cluster_number]---------", followed by +the information of LTR retrotransposons in the cluster. The columns for LTR +retrotransposons are as follows. + + 1. LTR_id: unique id of LTRs identified. It consist of two components, sequence file name and id in the file. For example, chr1_2 is the second LTR retrotransposon in the chr1 file. + 2. start position of 5’ LTR. + 3. end position of 5’ LTR. + 4. start position of 3’ LTR. + 5. end position of 3’ LTR. + 6. strand: + or -. + 7. length of 5’ LTR. + 8. length of 3’ LTR. + 9. length of the LTR retrotransposon. + 10. TSD on the left side of the LTR retotransposons. + 11. TSD on the right side of the LTR retrotransposons. + 12. di(tri)nucleotide on the left side of 5’LTR + 13. di(tri)nucleotide on the right side of 5’LTR + 14. di(tri)nucleotide on the left side of 3’LTR + 15. di(tri)nucleotide on the right side of 3’LTR + +B. MGEScan_nonLTR: + Upon completion, MGEScan-nonLTR generates the directory, "info" in the data directory you + specified. In this "info" directory, two sub-directories ("full" and "validation") are + generated. + + * The "full" directory is for storing sequences of elements. Each subdirectory in "full" + is the name of clade. In each directory of clade, the DNA sequences of nonLTRs identified + are listed. Each sequence is in fasta format. The header contains the position + information of TEs identified: [genome_file_name]_[start position in the sequence] + + For example, >chr1_333 means that this element start at 333bp in the "chr1" file. + + * The "validation" directory is for storing Q values. + In the files "en" and "rt", the first column corresponds to the element name and the last column Q value. + +License +============ +Copyright 2015. +You may redistribute this software under the terms of the GNU General Public License. + + +