# HG changeset patch # User hyungrolee # Date 1455525181 18000 # Node ID f9be9a13e01a0f959e2e6af93fb27053d31475d8 # Parent 493e3b73c15bebeb9f5cb88b1939c42299448df9 Deleted selected files diff -r 493e3b73c15b -r f9be9a13e01a ltr.xml --- a/ltr.xml Sat Jun 14 19:08:55 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,111 +0,0 @@ - - - - - de novo identification of LTR retroelements - - - - - - - mgescan.sh $input $input.name 3 $output L None None None $ltr_gff3 None $sw_rm "$scaffold" $min_dist $max_dist $min_len_ltr $max_len_ltr $ltr_sim_condition $cluster_sim_condition $len_condition $repeatmasker - - - - - - - - - - - - - - - - - - - - - - - - sw_rm == "Yes" - - - -Running the program -=================== - -To run MGEScan-LTR, follow the steps below, - -1. Specify options that you like to have: - - * Check repeatmasker if you want to preprocess - * Check scaffold if the input file has all scaffolds. - -2. Update values: - - * min_dist: minimum distance(bp) between LTRs. - * max_dist: maximum distance(bp) between LTRS - * min_len_ltr: minimum length(bp) of LTR. - * max_len_ltr: maximum length(bp) of LTR. - * ltr_sim_condition: minimum similarity(%) for LTRs in an element. - * cluster_sim_condition: minimum similarity(%) for LTRs in a cluster - * len_condition: minimum length(bp) for LTRs aligned in local alignment. - -4. Click 'Execute' - - * mask known repeats other than LTR retrotransposons - * identify LTRs - -Output -====== - -Upon completion, MGEScan-LTR generates a file ltr.out. This output file has information -about clusters and coordinates of LTR retrotransposons identified. Each cluster of LTR -retrotransposons starts with the head line of [cluster_number]---------, followed by -the information of LTR retrotransposons in the cluster. The columns for LTR -retrotransposons are as follows. - -1. LTR_id: unique id of LTRs identified. It consist of two components, sequence file name and id in the file. For example, chr1_2 is the second LTR retrotransposon in the chr1 file. -2. start position of 5 LTR. -3. end position of 5 LTR. -4. start position of 3 LTR. -5. end position of 3 LTR. -6. strand: + or -. -7. length of 5 LTR. -8. length of 3 LTR. -9. length of the LTR retrotransposon. -10. TSD on the left side of the LTR retotransposons. -11. TSD on the right side of the LTR retrotransposons. -12. di(tri)nucleotide on the left side of 5LTR -13. di(tri)nucleotide on the right side of 5LTR -14. di(tri)nucleotide on the left side of 3LTR -15. di(tri)nucleotide on the right side of 3LTR - -License -============ - -Copyright 2014 Mina Rho, Haixu Tang. -You may redistribute this software under the terms of the GNU General Public License. - - diff -r 493e3b73c15b -r f9be9a13e01a mgescan.sh --- a/mgescan.sh Sat Jun 14 19:08:55 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,126 +0,0 @@ -#!/bin/bash -# mgescan.sh $input $input.name 3 $output L None None None $ltr_gff3 None $sw_rm "$scaffold" $min_dist $max_dist $min_len_ltr $max_len_ltr $ltr_sim_condition $cluster_sim_condition $len_condition $repeatmasker -user_dir=/u/lee212 -#script=$user_dir/retrotminer/wazim/MGEScan1.1/run_MGEScan.pl -#script=$user_dir/retrotminer/wazim/MGEScan1.3.1/run_MGEScan2.pl -source $user_dir/virtualenv/retrotminer/bin/activate -script_program=`which python` -script=$user_dir/github/retrotminer/retrotminer/retrotminer.py -input_file=$1 -input_file_name=$2 -hmmsearch_version=$3 -output_file=$4 -program=$5 # N is nonLTR, L is LTR and B is both -# Optional output parameters for nonLTR -clade=$6 -en=$7 -rt=$8 -ltr_gff3=$9 -nonltr_gff3=${10} -#### for ltr between $11 and $20 -sw_rm=${11} -scaffold=${12} -min_dist=${13} -max_dist=${14} -min_len_ltr=${15} -max_len_ltr=${16} -ltr_sim_condition=${17} -cluster_sim_condition=${18} -len_condition=${19} -repeatmasker=${20} - -# /nfs/nfs4/home/lee212/retrotminer/galaxy-dist/tools/retrotminer/find_ltr.sh /nfs/nfs4/home/lee212/retrotminer/galaxy-dist/database/files/000/dataset_1.dat /nfs/nfs4/home/lee212/retrotminer/galaxy-dist/database/files/000/dataset_3.dat - -#load env? -source $user_dir/.bashrc -source $user_dir/.bash_profile - -#set path for transeq -export PATH=$user_dir/retrotminer/EMBOSS/bin:/usr/bin:$PATH - -#move to the working directory -work_dir=`dirname $script` -cd $work_dir - -#create directory for input and output -mkdir -p input -t_dir=`mktemp -p input -d` #relative path -input_dir="$work_dir/$t_dir/seq" # full path -output_dir="$work_dir/$t_dir/data" -mkdir -p $input_dir -mkdir -p $output_dir - -#make a copy of input -/bin/cp $input_file $input_dir/$input_file_name - -if [ "2" == "$hmmsearch_version" ] -then - export PATH=$user_dir/retrotminer/HMMER2.0/bin:$PATH -else - export PATH=/usr/bin:$PATH -fi - -if [ "$program" == "L" ] -then - program_name="ltr" -else - programname="nonltr" -fi - -#run -$script_program $script $program_name $input_dir/ --output=$output_dir/ #-hmmerv=$hmmsearch_version -sw_rm=${11} -scaffold=${12} -min_dist=${13} -max_dist=${14} -min_len_ltr=${15} -max_len_ltr=${16} -ltr_sim_condition=${17} -cluster_sim_condition=${18} -len_condition=${19} -#/usr/bin/perl $script -genome=$input_dir/ -data=$output_dir/ -hmmerv=$hmmsearch_version -program=$program -sw_rm=${11} -scaffold=${12} -min_dist=${13} -max_dist=${14} -min_len_ltr=${15} -max_len_ltr=${16} -ltr_sim_condition=${17} -cluster_sim_condition=${18} -len_condition=${19} - -#RES=`ssh -i $user_dir/.ssh/.internal silo.cs.indiana.edu "/usr/bin/perl $script -genome=$input_dir/ -data=$output_dir/ -hmmerv=$hmmsearch_version -program=$program > /dev/null"` - -#make a copy of output -if [ "$program" != "N" ] -then - /bin/cp $output_dir/ltr/ltr.out $output_file - if [ "$ltr_gff3" != "None" ] - then - /bin/cp $output_dir/ltr/ltr.gff3 $ltr_gff3 - fi - - if [ "$repeatmasker" != "None" ] - then - # chr2L.fa.cat.gz chr2L.fa.masked chr2L.fa.out chr2L.fa.out.pos chr2L.fa.tbl - /bin/cp $output_dir/repeatmasker/${input_file_name}.out $repeatmasker - fi -fi -if [ "$program" != "L" ] -then - - #compressed_file=$output_dir/$RANDOM.tar.gz - #/bin/tar cvzfP $compressed_file $output_dir/info - #/bin/cp $compressed_file $output_file - /bin/cp $output_dir/info/full/*/* $clade - /bin/cp $output_dir/info/validation/en $en - /bin/cp $output_dir/info/validation/rt $rt - if [ "$nonltr_gff3" != "None" ] - then - /bin/cp $output_dir/info/nonltr.gff3 $nonltr_gff3 - # nonltr.gff3 - ##gff-version 3 - #chr2L.fa MGEScan_nonLTR mobile_genetic_element 19670384 19676921 . . . ID=chr2L.fa_19670384 - #chr2L.fa MGEScan_nonLTR mobile_genetic_element 17689430 17695994 . . . ID=chr2L.fa_17689430 - #chr2L.fa MGEScan_nonLTR mobile_genetic_element 11897186 11903717 . . . ID=chr2L.fa_11897186 - #chr2L.fa MGEScan_nonLTR mobile_genetic_element 49574 56174 . . . ID=chr2L.fa_49574 - fi - -#else - # Both LTR, nonLTR executed - #compressed_file=$output_dir/$RANDOM.tar.gz - #/bin/tar cvzfP $compressed_file $output_dir - #/bin/cp $compressed_file $output_file -fi - -# delete temp directory -if [ $? -eq 0 ] -then - rm -rf $work_dir/$t_dir - #echo -else - #echo cp -pr $work_dir/$t_dir $work_dir/error-cases/ - cp -pr $work_dir/$t_dir $work_dir/error-cases/ -fi diff -r 493e3b73c15b -r f9be9a13e01a mgescan.xml --- a/mgescan.xml Sat Jun 14 19:08:55 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,101 +0,0 @@ - - - - - MGEScan - - - mgescan.sh $input '$input.name' 3 $output $program $clade $qvalue_en $qvalue_rt $ltr_gff3 $nonltr_gff3 - - - - - - - - - - - - - - program != "N" - - - program != "L" - - - program != "L" - - - program != "L" - - - program != "N" - - - program != "L" - - - - -Running the program -=================== - -To run MGEScan, select input genome data in From select box, and select program either LTR, nonLTR or both. - -Click 'Execute' button. - -If you like to have more options to run LTR or nonLTR progrma, use separated tools on the left panel. -In LTR > MGEScan-LTR, preprocessing by repeatmasker and setting other variables are available e.g. distance(bp) between LTRs. - -Output -============ -A. MGEScan_LTR: -Upon completion, MGEScan-LTR generates a file "ltr.out". This output file has information -about clusters and coordinates of LTR retrotransposons identified. Each cluster of LTR -retrotransposons starts with the head line of "[cluster_number]---------", followed by -the information of LTR retrotransposons in the cluster. The columns for LTR -retrotransposons are as follows. - -1. LTR_id: unique id of LTRs identified. It consist of two components, sequence file name and id in the file. For example, chr1_2 is the second LTR retrotransposon in the chr1 file. -2. start position of 5’ LTR. -3. end position of 5’ LTR. -4. start position of 3’ LTR. -5. end position of 3’ LTR. -6. strand: + or -. -7. length of 5’ LTR. -8. length of 3’ LTR. -9. length of the LTR retrotransposon. -10. TSD on the left side of the LTR retotransposons. -11. TSD on the right side of the LTR retrotransposons. -12. di(tri)nucleotide on the left side of 5’LTR -13. di(tri)nucleotide on the right side of 5’LTR -14. di(tri)nucleotide on the left side of 3’LTR -15. di(tri)nucleotide on the right side of 3’LTR - -B. MGEScan_nonLTR: - Upon completion, MGEScan-nonLTR generates the directory, "info" in the data directory you - specified. In this "info" directory, two sub-directories ("full" and "validation") are - generated. - - - The "full" directory is for storing sequences of elements. Each subdirectory in "full" - is the name of clade. In each directory of clade, the DNA sequences of nonLTRs identified - are listed. Each sequence is in fasta format. The header contains the position - information of TEs identified: - [genome_file_name]_[start position in the sequence] - - For example, >chr1_333 means that this element start at 333bp in the "chr1" file. - - - The "validation" directory is for storing Q values. In the files "en" and "rt", the first column corresponds to the element name and the last column Q value. - -License -============ -Copyright 2014 Mina Rho, Haixu Tang. -You may redistribute this software under the terms of the GNU General Public License. - - - diff -r 493e3b73c15b -r f9be9a13e01a nonltr.xml --- a/nonltr.xml Sat Jun 14 19:08:55 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,61 +0,0 @@ - - - - - computational identification and classification of non-LTR retroelements - - - - - mgescan.sh $input $input.name 3 None N $clade $qvalue_en $qvalue_rt None $nonltr_gff3 - - - - - - - - - - - - - - -Running the program -=================== - -To run MGEScan-nonLTR, follow the steps below: - -1. Select genome files a select box. You can upload your genome files through 'Get Data' at Tools menu bar. - -2. Click 'Execute' button. This tool reads your genome files and runs the whole process. - -Output -====== - -Upon completion, MGEScan-nonLTR generates output, "info" in the data directory you specified. In this "info" directory, two sub-directories ("full" and "validation") are generated. - - - The "full" directory is for storing sequences of elements. Each subdirectory in "full" is the name of clade. In each directory of clade, the DNA sequences of nonLTRs identified are listed. Each sequence is in fasta format. The header contains the position information of TEs identified, [genome_file_name]_[start position in the sequence] - - - For example, >chr1_333 means that this element start at 333bp in the "chr1" file. - The "validation" directory is for storing Q values. In the files "en" and "rt", the first column corresponds to the element name and the last column Q value. - -License -============ -Copyright 2014 Mina Rho, Haixu Tang. -You may redistribute this software under the terms of the GNU General Public License. - - -