Mercurial > repos > idot > fastx_toolkit2
diff fastq_quality_boxplot.xml @ 0:78a7d28f2a15 draft
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author | idot |
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date | Wed, 10 Jul 2013 06:13:48 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastq_quality_boxplot.xml Wed Jul 10 06:13:48 2013 -0400 @@ -0,0 +1,54 @@ +<tool id="cshl_fastq_quality_boxplot" name="Draw quality score boxplot"> + <description></description> + + <command>fastq_quality_boxplot_graph.sh -t '$input.name' -i $input -o '$output'</command> + + <inputs> + <param format="txt" name="input" type="data" label="Statistics report file" help="output of 'FASTQ Statistics' tool" /> + </inputs> + + <outputs> + <data format="png" name="output" metadata_source="input" + /> + </outputs> +<help> + +**What it does** + +Creates a boxplot graph for the quality scores in the library. + +.. class:: infomark + +**TIP:** Use the **FASTQ Statistics** tool to generate the report file needed for this tool. + +----- + +**Output Examples** + +* Black horizontal lines are medians +* Rectangular red boxes show the Inter-quartile Range (IQR) (top value is Q3, bottom value is Q1) +* Whiskers show outlier at max. 1.5*IQR + + +An excellent quality library (median quality is 40 for almost all 36 cycles): + +.. image:: ../static/fastx_icons/fastq_quality_boxplot_1.png + + +A relatively good quality library (median quality degrades towards later cycles): + +.. image:: ../static/fastx_icons/fastq_quality_boxplot_2.png + +A low quality library (median drops quickly): + +.. image:: ../static/fastx_icons/fastq_quality_boxplot_3.png + +------ + +This tool is based on `FASTX-toolkit`__ by Assaf Gordon. + + .. __: http://hannonlab.cshl.edu/fastx_toolkit/ + +</help> +</tool> +<!-- FASTQ-Quality-Boxplot is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->