Mercurial > repos > idot > fastx_toolkit2
diff fastx_renamer.xml @ 0:78a7d28f2a15 draft
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author | idot |
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date | Wed, 10 Jul 2013 06:13:48 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastx_renamer.xml Wed Jul 10 06:13:48 2013 -0400 @@ -0,0 +1,75 @@ +<tool id="cshl_fastx_renamer" name="Rename sequences" version="0.0.11" > + <description></description> + <command> +cat '$input' | +fastx_renamer +#if $input.ext == "fastqsanger": + -Q 33 +#elif $input.ext == "fastq": + -Q 64 +#end if + -n $TYPE -o '$output' -v +</command> + <inputs> + <param format="fastq,fastqsanger,fasta" name="input" type="data" label="FASTQ/A Library to rename" /> + + <param name="TYPE" type="select" label="Rename sequence identifiers to"> + <option value="SEQ">Nucleotides sequence</option> + <option value="COUNT">Numeric Counter</option> + </param> + </inputs> + <tests> + <test> + <param name="input" value="fastx_renamer1.fastq" ftype="fastq"/> + <param name="TYPE" value="SEQ" /> + <output name="output" file="fastx_renamer1.out" /> + </test> + </tests> + + <outputs> + <data format="input" name="output" metadata_source="input" /> + </outputs> + +<help> + +**What it does** + +This tool renames the sequence identifiers in a FASTQ/A file. + +.. class:: infomark + +Use this tool at the beginning of your workflow, as a way to keep the original sequence (before trimming, clipping, barcode-removal, etc). + +-------- + +**Example** + +The following Solexa-FASTQ file:: + + @CSHL_4_FC042GAMMII_2_1_517_596 + GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT + +CSHL_4_FC042GAMMII_2_1_517_596 + 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 + +Renamed to **nucleotides sequence**:: + + @GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT + GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT + +GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT + 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 + +Renamed to **numeric counter**:: + + @1 + GGTCAATGATGAGTTGGCACTGTAGGCACCATCAAT + +1 + 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 14 40 40 40 40 40 36 40 13 14 24 24 9 24 9 40 10 10 15 40 + +------ + +This tool is based on `FASTX-toolkit`__ by Assaf Gordon. + + .. __: http://hannonlab.cshl.edu/fastx_toolkit/ +</help> +</tool> +<!-- FASTX-renamer is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->