Mercurial > repos > idot > fastx_toolkit2
diff fastx_reverse_complement.xml @ 0:78a7d28f2a15 draft
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author | idot |
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date | Wed, 10 Jul 2013 06:13:48 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastx_reverse_complement.xml Wed Jul 10 06:13:48 2013 -0400 @@ -0,0 +1,68 @@ +<tool id="cshl_fastx_reverse_complement" name="Reverse-Complement"> + <description></description> + <command> +cat '$input' | +fastx_reverse_complement +#if $input.ext == "fastqsanger": + -Q 33 +#elif $input.ext == "fastq": + -Q 64 +#end if + -v -o '$output' +</command> + <inputs> + <param format="fasta,fastq,fastqsanger" name="input" type="data" label="Library to reverse-complement" /> + </inputs> + + <tests> + <test> + <!-- Reverse-complement a FASTA file --> + <param name="input" value="fastx_rev_comp1.fasta" /> + <output name="output" file="fastx_reverse_complement1.out" /> + </test> + <test> + <!-- Reverse-complement a FASTQ file --> + <param name="input" value="fastx_rev_comp2.fastq" /> + <output name="output" file="fastx_reverse_complement2.out" /> + </test> + </tests> + + <outputs> + <data format="input" name="output" metadata_source="input" + /> + </outputs> + +<help> +**What it does** + +This tool reverse-complements each sequence in a library. +If the library is a FASTQ, the quality-scores are also reversed. + +-------- + +**Example** + +Input FASTQ file:: + + @CSHL_1_FC42AGWWWXX:8:1:3:740 + TGTCTGTAGCCTCNTCCTTGTAATTCAAAGNNGGTA + +CSHL_1_FC42AGWWWXX:8:1:3:740 + 33 33 33 34 33 33 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 27 21 27 33 32 31 29 26 24 5 5 15 17 27 26 + + +Output FASTQ file:: + + @CSHL_1_FC42AGWWWXX:8:1:3:740 + TACCNNCTTTGAATTACAAGGANGAGGCTACAGACA + +CSHL_1_FC42AGWWWXX:8:1:3:740 + 26 27 17 15 5 5 24 26 29 31 32 33 27 21 27 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 33 33 34 33 33 33 + +------ + +This tool is based on `FASTX-toolkit`__ by Assaf Gordon. + + .. __: http://hannonlab.cshl.edu/fastx_toolkit/ + +</help> +</tool> +<!-- FASTX-reverse-complement is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->