Mercurial > repos > ieguinoa > data_manager_fetch_tx2gene
view data_manager/data_manager_fetch_tx2gene.py @ 5:c380b7da5b65 draft default tip
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author | ieguinoa |
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date | Mon, 07 Jun 2021 16:33:53 +0000 |
parents | d71f65b854de |
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#!/usr/bin/env python #Dan Blankenberg import sys import os import tempfile import shutil import optparse from ftplib import FTP import tarfile import zipfile import gzip import bz2 import subprocess try: # For Python 3.0 and later from urllib.request import urlopen from io import BytesIO as StringIO from io import UnsupportedOperation except ImportError: # Fall back to Python 2's urllib2 from urllib2 import urlopen from StringIO import StringIO UnsupportedOperation = AttributeError from json import loads, dumps CHUNK_SIZE = 2**20 # 1mb DATA_TABLE_NAME = 'tx2gene_table' def cleanup_before_exit( tmp_dir ): if tmp_dir and os.path.exists( tmp_dir ): shutil.rmtree( tmp_dir ) def stop_err(msg): sys.stderr.write(msg) sys.exit(1) def get_dbkey_dbname_id_name( params, dbkey_description=None ): # dbkey = params['param_dict']['dbkey_source']['dbkey'] #TODO: ensure sequence_id is unique and does not already appear in location file sequence_id = params['param_dict']['sequence_id'] if not sequence_id: sequence_id = dbkey #uuid.uuid4() generate and use an uuid instead? # if params['param_dict']['dbkey_source']['dbkey_source_selector'] == 'new': # dbkey_name = params['param_dict']['dbkey_source']['dbkey_name'] # if not dbkey_name: # dbkey_name = dbkey # else: # dbkey_name = None dbkey = params['param_dict']['dbkey'] dbkey_name = dbkey_description sequence_name = params['param_dict']['sequence_name'] if not sequence_name: sequence_name = dbkey_description if not sequence_name: sequence_name = dbkey return dbkey, dbkey_name, sequence_id, sequence_name def _get_files_in_ftp_path( ftp, path ): path_contents = [] ftp.retrlines( 'MLSD %s' % ( path ), path_contents.append ) return [ line.split( ';' )[ -1 ].lstrip() for line in path_contents ] def _get_stream_readers_for_tar( fh, tmp_dir ): fasta_tar = tarfile.open( fileobj=fh, mode='r:*' ) return [x for x in [fasta_tar.extractfile(member) for member in fasta_tar.getmembers()] if x] def _get_stream_readers_for_zip( fh, tmp_dir ): """ Unpacks all archived files in a zip file. Individual files will be concatenated (in _stream_fasta_to_file) """ fasta_zip = zipfile.ZipFile( fh, 'r' ) rval = [] for member in fasta_zip.namelist(): fasta_zip.extract( member, tmp_dir ) rval.append( open( os.path.join( tmp_dir, member ), 'rb' ) ) return rval def _get_stream_readers_for_gzip( fh, tmp_dir ): return [ gzip.GzipFile( fileobj=fh, mode='rb') ] def _get_stream_readers_for_bz2( fh, tmp_dir ): return [ bz2.BZ2File( fh.name, 'rb') ] def convert_to_tx2gene( rscript_gff_to_tx2gene, fasta_filename, file_type, params ): if file_type == 'tx2gene': return #no need to extract tx2gene table #print file_type #If the file is actually a GFF/GTF file then extract the tx2gene gff_temp_filename = tempfile.NamedTemporaryFile().name shutil.move(fasta_filename, gff_temp_filename) args= ['Rscript'] args.append(rscript_gff_to_tx2gene) args.extend(['-x',gff_temp_filename]) args.extend(['-o',fasta_filename]) args.extend(['-t',file_type]) tmp_stderr = tempfile.NamedTemporaryFile( prefix = "tmp-stderr" ) return_code = subprocess.call( args=args, shell=False, stderr=tmp_stderr.fileno() ) #return_code = subprocess.call( args=args, shell=False, stderr=None) if return_code: tmp_stderr.flush() tmp_stderr.seek(0) print >> sys.stderr, "Error in process call" while True: chunk = tmp_stderr.read( CHUNK_SIZE ) if not chunk: break sys.stderr.write( chunk ) sys.exit( return_code ) tmp_stderr.close() def _download_file(start, fh): tmp = tempfile.NamedTemporaryFile() tmp.write(start) tmp.write(fh.read()) tmp.flush() tmp.seek(0) return tmp def get_stream_reader(fh, tmp_dir): """ Check if file is compressed and return correct stream reader. If file has to be downloaded, do it now. """ magic_dict = { b"\x1f\x8b\x08": _get_stream_readers_for_gzip, b"\x42\x5a\x68": _get_stream_readers_for_bz2, b"\x50\x4b\x03\x04": _get_stream_readers_for_zip, } start_of_file = fh.read(CHUNK_SIZE) try: fh.seek(0) except UnsupportedOperation: # This is if fh has been created by urlopen fh = _download_file(start_of_file, fh) for k,v in magic_dict.items(): if start_of_file.startswith(k): return v(fh, tmp_dir) try: # Check if file is tar file if tarfile.open(fileobj=StringIO(start_of_file)): return _get_stream_readers_for_tar(fh, tmp_dir) except tarfile.ReadError: pass return fh def add_fasta_to_table(rscript_gff_to_tx2gene, data_manager_dict, fasta_readers, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, params): for data_table_name, data_table_entry in _stream_fasta_to_file(rscript_gff_to_tx2gene, fasta_readers, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, params ): if data_table_entry: _add_data_table_entry( data_manager_dict, data_table_entry, data_table_name ) def download_from_url(rscript_gff_to_tx2gene, data_manager_dict, params, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, tmp_dir ): urls = filter( bool, map( lambda x: x.strip(), params['param_dict']['reference_source']['user_url'].split( '\n' ) ) ) fasta_readers = [ get_stream_reader(urlopen( url ), tmp_dir) for url in urls ] add_fasta_to_table(rscript_gff_to_tx2gene,data_manager_dict, fasta_readers, target_directory, dbkey, dbkey_name, sequence_id,sequence_name, params) def download_from_history(rscript_gff_to_tx2gene, data_manager_dict, params, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, tmp_dir ): #TODO: allow multiple FASTA input files input_filename = params['param_dict']['reference_source']['input_fasta'] if isinstance( input_filename, list ): fasta_readers = [ get_stream_reader(open(filename, 'rb'), tmp_dir) for filename in input_filename ] else: fasta_readers = get_stream_reader(open(input_filename), tmp_dir) add_fasta_to_table(rscript_gff_to_tx2gene,data_manager_dict, fasta_readers, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, params) def copy_from_directory(rscript_gff_to_tx2gene, data_manager_dict, params, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, tmp_dir ): input_filename = params['param_dict']['reference_source']['fasta_filename'] create_symlink = params['param_dict']['reference_source']['create_symlink'] == 'create_symlink' if create_symlink: data_table_entries = _create_symlink( input_filename, target_directory, dbkey, dbkey_name, sequence_id, sequence_name ) else: if isinstance( input_filename, list ): fasta_readers = [ get_stream_reader(open(filename, 'rb'), tmp_dir) for filename in input_filename ] else: fasta_readers = get_stream_reader(open(input_filename), tmp_dir) data_table_entries = _stream_fasta_to_file(rscript_gff_to_tx2gene, fasta_readers, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, params ) for data_table_name, data_table_entry in data_table_entries: if data_table_entry: _add_data_table_entry( data_manager_dict, data_table_entry, data_table_name ) def _add_data_table_entry( data_manager_dict, data_table_entry, data_table_name ): data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} ) data_manager_dict['data_tables'][data_table_name] = data_manager_dict['data_tables'].get( DATA_TABLE_NAME, [] ) data_manager_dict['data_tables'][data_table_name].append( data_table_entry ) return data_manager_dict def _stream_fasta_to_file( rscript_gff_to_tx2gene, fasta_stream, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, params, close_stream=True ): fasta_base_filename = "%s_tx2gene.tab" % sequence_id fasta_filename = os.path.join( target_directory, fasta_base_filename ) with open( fasta_filename, 'wb+' ) as fasta_writer: if isinstance( fasta_stream, list ) and len( fasta_stream ) == 1: fasta_stream = fasta_stream[0] if isinstance( fasta_stream, list ): last_char = None for fh in fasta_stream: if last_char not in [ None, '\n', '\r', b'\n', b'\r' ]: fasta_writer.write( b'\n' ) while True: data = fh.read( CHUNK_SIZE ) if data: fasta_writer.write( data ) last_char = data[-1] else: break if close_stream: fh.close() else: while True: data = fasta_stream.read( CHUNK_SIZE ) if data: fasta_writer.write( data ) else: break if close_stream: fasta_stream.close() convert_to_tx2gene( rscript_gff_to_tx2gene,fasta_filename, params['param_dict']['file_type'], params ) return [ ( DATA_TABLE_NAME, dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_filename ) ) ] def compute_fasta_length( fasta_file, out_file, keep_first_word=False ): infile = fasta_file out = open( out_file, 'w') fasta_title = '' seq_len = 0 first_entry = True for line in open( infile ): line = line.strip() if not line or line.startswith( '#' ): continue if line[0] == '>': if first_entry == False: if keep_first_word: fasta_title = fasta_title.split()[0] out.write( "%s\t%d\n" % ( fasta_title[ 1: ], seq_len ) ) else: first_entry = False fasta_title = line seq_len = 0 else: seq_len += len(line) # last fasta-entry if keep_first_word: fasta_title = fasta_title.split()[0] out.write( "%s\t%d\n" % ( fasta_title[ 1: ], seq_len ) ) out.close() def _create_symlink( input_filename, target_directory, dbkey, dbkey_name, sequence_id, sequence_name ): fasta_base_filename = "%s.fa" % sequence_id fasta_filename = os.path.join( target_directory, fasta_base_filename ) os.symlink( input_filename, fasta_filename ) return [ ( DATA_TABLE_NAME, dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_filename ) ) ] REFERENCE_SOURCE_TO_DOWNLOAD = dict( url=download_from_url, history=download_from_history, directory=copy_from_directory ) def main(): #Parse Command Line parser = optparse.OptionParser() parser.add_option( '-d', '--dbkey_description', dest='dbkey_description', action='store', type="string", default=None, help='dbkey_description' ) parser.add_option( '-b', '--base_dir', dest='base_dir', action='store', type='string', default=None, help='base_dir') parser.add_option( '-t', '--type', dest='file_type', action='store', type='string', default=None, help='file_type') (options, args) = parser.parse_args() filename = args[0] #global DATA_TABLE_NAME rscript_gff_to_tx2gene=os.path.join( options.base_dir, 'get_tx2gene_table.R') #input_type='gff_gtf' #if options.file_type != 'gff_gtf': # file_type='tx2gene' params = loads( open( filename ).read() ) target_directory = params[ 'output_data' ][0]['extra_files_path'] os.mkdir( target_directory ) data_manager_dict = {} dbkey, dbkey_name, sequence_id, sequence_name = get_dbkey_dbname_id_name( params, dbkey_description=options.dbkey_description ) if dbkey in [ None, '', '?' ]: raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) ) # Create a tmp_dir, in case a zip file needs to be uncompressed tmp_dir = tempfile.mkdtemp() #Fetch the input file try: REFERENCE_SOURCE_TO_DOWNLOAD[ params['param_dict']['reference_source']['reference_source_selector'] ]( rscript_gff_to_tx2gene, data_manager_dict, params, target_directory, dbkey, dbkey_name, sequence_id, sequence_name, tmp_dir) finally: cleanup_before_exit(tmp_dir) #save info to json file open( filename, 'wb' ).write( dumps( data_manager_dict ).encode() ) if __name__ == "__main__": main()