Mercurial > repos > ieguinoa > ena_webin_cli
diff ena_consensus_submit.xml @ 0:e25357392813 draft
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author | ieguinoa |
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date | Tue, 18 May 2021 16:30:52 +0000 |
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children | f24eb2f2cb0c |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ena_consensus_submit.xml Tue May 18 16:30:52 2021 +0000 @@ -0,0 +1,159 @@ +<tool id="ena_consensus_submit" name="Submit consensus sequence to ENA" version="0.1.0" python_template_version="3.5"> + <macros> + <token name="@VERSION@">3.7.0</token> + </macros> + <requirements> + <requirement type="package" version="@VERSION@">ena-webin-cli</requirement> + <requirement type="package" version="1.76">biopython</requirement> + <requirement type="package" version="5.3">pyyaml</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + +webin_id=`grep 'username' $credentials | cut -f2,2`; +if [ "\$webin_id" = "" ]; then + ## No credentials in user defined preferences + ## Fallback to global defined credentials (if exist) + #import os + #if os.path.isfile(os.environ.get('GALAXY_ENA_SECRETS', '')): + credentials_path=\${GALAXY_ENA_SECRETS}; + webin_id=`grep 'username' \$GALAXY_ENA_SECRETS | cut -d' ' -f2,2`; + password=`grep 'password' \$GALAXY_ENA_SECRETS | cut -d' ' -f2,2`; + if [ "\$webin_id" = "" ]; then + echo "No global credentials defined. Check your GALAXY_ENA_SECRETS file or set your credentials via: User -> Preferences -> Manage Information"; + exit 1; + fi; + #else: + echo "No ENA credentials defined. Set your credentials via: User -> Preferences -> Manage Information"; + exit 1; + #end if +else + password=`grep 'password' $credentials | cut -f2,2`; +fi; + +## if it is still running at this point then the webin_id and password are set + +## create a manifaste base file with the parameters that are identical for all sequences that will be submited (if submitting a multifasta) +#set $manifest_base = 'manifest_base.tab' +mkdir manifests; +mkdir fasta; + +echo -e "ASSEMBLY_TYPE\t$assembly_type" >> $manifest_base; +echo -e "COVERAGE\t$coverage" >> $manifest_base; +echo -e "PROGRAM\t$assembly_program" >> $manifest_base; +echo -e "MINGAPLENGTH\t$min_gap_length" >> $manifest_base; +echo -e "MOLECULETYPE\t$molecule_type" >> $manifest_base; + +#if $metadata_file_or_form.metadata_format == "file": + ## process the input tables, this creates an intermediate file with information + python3 '$__tool_directory__/process_input.py' $metadata_file_or_form.ena_receipt $genome_fasta './manifests' './fasta' $manifest_base; + center_name=`grep 'center_name' $metadata_file_or_form.ena_receipt | cut -f2,2 | tr -d '\n'`; +#else: + #set $generated_manifest='./manifests/generated_manifest.txt' + cp $manifest_base $generated_manifest + $study_id = $metadata_file_or_form.study_accession + $sample_id = $metadata_file_or_form.sample_accession + echo "STUDY\t$study_id" > $generated_manifest; + echo "SAMPLE\t$sample_id" >> $generated_manifest; + center_name=$metadata_file_or_form.center_name; + echo "ASSEMBLY_NAME\t$metadata_file_or_form.assembly_name" >> $generated_manifest; + echo "PLATFORM\t$platform_name" >> $generated_manifest; +#end if + + +#if $metadata_file_or_form.metadata_format == "file": + ## iterate over the list of manifest - fasta generated by the process_input + ## in case of errors, this list is empty + while read line; do + manifest=`echo \$line | cut -d' ' -f1,1`; + ena-webin-cli + -context genome + -userName \$webin_id + -password \$password + -centerName \$center_name + -manifest \$manifest + -inputDir "./fasta" + #if $dry_run == "true": + -validate + #end if + ; + done < submit_list.tab + +#else: + gzip -c $genome_fasta > consensus.fasta.gz; + echo -e "FASTA\tconsensus.fasta.gz" >> $generated_manifest; + ena-webin-cli + -context genome + -userName \$webin_id + -password \$password + -centerName '\$center_name' + -manifest $generated_manifest + -inputDir "./" + #if $submit_test == "true": + -test + #end if + #if $dry_run == "true": + -validate + #else: + -submit + #end if +#end if +]]></command> + <configfiles> + <configfile name="credentials"><![CDATA[ +#set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip() +#set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip() +#if $webin_id != "": + username\t"$webin_id" + password\t"$password" +#end if + ]]></configfile> + </configfiles> +<inputs> + <param name="submit_test" type="boolean" default="False" label="Submit to test server" help="Suggested to test metadata format" /> + <param name="dry_run" type="boolean" default="False" label="Validate files and metadata but do not submit" help="Generate input files and run Webin-CLI with -validate option."/> + <param name="genome_fasta" type="data" label="Select the consensus sequence assembly file" format="fasta"/> + <param name="assembly_type" type="select" label="Assembly type"> + <option value="clone">Clone</option> + <option value="isolate">Isolate</option> + </param> + <param name="assembly_program" type="text" optional="False" label="Assembly program"/> + <param name="molecule_type" type="select" label="Molecule type"> + <option value="genomic RNA" selected="True">genomic RNA</option> + <option value="viral cRNA">viral cRNA</option> + <option value="genomic DNA">genomic DNA</option> + </param> + <param name="min_gap_length" type="text" optional="True" label="Minimum gap length"/> + <param name="coverage" type="float" optional="False" value="10000" label="Coverage"/> + <conditional name="metadata_file_or_form"> + <param name="metadata_format" type="select" label="Select the method to load study and sample metadata"> + <option value="file" selected="True">I used Galaxy ENA upload tool for raw data submission, parse my submission receipt</option> + <option value="form">Fill in required metadata for linking</option> + </param> + <when value="file"> + <param type="data" format="txt" name="ena_receipt" label="Submission receipt obtained from ENA upload tool"/> + </when> + <when value="form"> + <param name="assembly_name" type="text" optional="False" label="Assembly name"/> + <param name="study_accession" type="text" optional="False" label="Study accession or unique name (alias)"/> + <param name="sample_accession" type="text" optional="False" label="Sample accession or unique name (alias)"/> + <param name="sequencing_platform" type="text" optional="False" label="Sequencing platform"/> + <param name="description" type="text" optional="True" value="" label="Description" help="Free text description of the genome assembly (optional)"/> + <param name="center_name" type="text" optional="False" label="Center name"/> + </when> + </conditional> + </inputs> + <outputs> + <collection name="generated_manifests" type="list" label="Generated manifests"> + <!--<discover_datasets pattern="__name_and_ext__" directory="manifests" />--> + <discover_datasets pattern="(?P<designation>.+)\.manifest\.txt$" ext="txt" directory="manifests" /> + </collection> + <collection name="manifests_reports" type="list" label="Manifests reports"> + <!--[><discover_datasets pattern="__name_and_ext__" directory="manifests" /><]--> + <discover_datasets pattern="(?P<designation>.+)\.manifest\.txt\.report$" ext="txt" directory="manifests" /> + </collection> + <data name="webin_cli_report" label="ENA submission receipt" format="txt" from_work_dir="manifests/webin-cli.report"/> + </outputs> + <help><![CDATA[ + TODO: Fill in help. + ]]></help> +</tool>