Mercurial > repos > imgteam > imagej2_analyze_particles_binary
view imagej2_adjust_threshold_binary_jython_script.py @ 2:ae4ae9c5c56c draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
author | imgteam |
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date | Sun, 05 Nov 2023 14:25:37 +0000 |
parents | 1dd5396c734a |
children | 862af85a50ec |
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import sys from ij import IJ # Fiji Jython interpreter implements Python 2.5 which does not # provide support for argparse. error_log = sys.argv[-10] input_file = sys.argv[-9] threshold_min = float(sys.argv[-8]) threshold_max = float(sys.argv[-7]) method = sys.argv[-6] display = sys.argv[-5] black_background = sys.argv[-4] == "yes" # TODO: this is not being used. stack_histogram = sys.argv[-3] == "yes" output_filename = sys.argv[-2] output_datatype = sys.argv[-1] # Open the input image file. input_image_plus = IJ.openImage(input_file) # Create a copy of the image. input_image_plus_copy = input_image_plus.duplicate() image_processor_copy = input_image_plus_copy.getProcessor() # Convert image to binary if necessary. if not image_processor_copy.isBinary(): # Convert the image to binary grayscale. IJ.run( input_image_plus_copy, "Make Binary", "iterations=1 count=1 edm=Overwrite do=Nothing", ) # Set the options. if black_background: method_str = "%s dark" % method else: method_str = method IJ.setAutoThreshold(input_image_plus_copy, method_str) if display == "red": display_mode = "Red" elif display == "bw": display_mode = "Black & White" elif display == "over_under": display_mode = "Over/Under" IJ.setThreshold(input_image_plus_copy, threshold_min, threshold_max, display_mode) # Run the command. IJ.run(input_image_plus_copy, "threshold", "") # Save the ImagePlus object as a new image. IJ.saveAs(input_image_plus_copy, output_datatype, output_filename)