Mercurial > repos > imgteam > imagej2_analyze_skeleton
diff imagej2_analyze_skeleton.xml @ 0:bb84f130a4e1 draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
author | imgteam |
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date | Tue, 17 Sep 2019 17:04:59 -0400 |
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children | 3272e0439968 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/imagej2_analyze_skeleton.xml Tue Sep 17 17:04:59 2019 -0400 @@ -0,0 +1,127 @@ +<?xml version='1.0' encoding='UTF-8'?> +<tool id="imagej2_analyze_skeleton" name="Analyze skeleton" version="@WRAPPER_VERSION@.0"> + <description></description> + <macros> + <import>imagej2_macros.xml</import> + </macros> + <expand macro="fiji_requirements" /> + <command> +<![CDATA[ + python $__tool_directory__/imagej2_analyze_skeleton.py + --input "$input" + --input_datatype $input.ext + --black_background $black_background + --prune_cycle_method $prune_cycle_method + --prune_ends $prune_ends + --calculate_largest_shortest_path $calculate_largest_shortest_path + ##TODO: seel below--show_detailed_info $show_detailed_info + --jython_script $__tool_directory__/imagej2_analyze_skeleton_jython_script.py + --output "$output" +]]> + </command> + <inputs> + <param format="bmp,eps,gif,jpg,pcx,pgm,png,psd,tiff" name="input" type="data" label="Select grayscale image"/> + <expand macro="black_background_param" /> + <param name="prune_cycle_method" type="select" label="Prune cycle method"> + <option value="none" selected="True">None</option> + <option value="shortest_branch">Shortest branch</option> + <option value="lowest_intensity_voxel">Lowest intensity voxel</option> + <option value="lowest_intensity_branch">Lowest intensity branch</option> + </param> + <param name="prune_ends" type="select" label="Prune ends"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <param name="calculate_largest_shortest_path" type="select" label="Calculate largest shortest path"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <!-- TODO: Uncomment this option when the tool has been enhanced to use the + future "Display labeled skeletons" option. See http://fiji.sc/AnalyzeSkeleton. + <param name="show_detailed_info" type="select" label="Show detailed info"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + --> + </inputs> + <outputs> + <data name="output" format="tabular" label="${tool.name} on ${on_string}" /> + </outputs> + <tests> + <test> + <param name="input" value="skeletonized_blobs.gif" /> + <param name="input_datatype" value="gif" /> + <output name="output" file="basic.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_clown.jpg" /> + <param name="input_datatype" value="jpg" /> + <param name="prune_cycle_method" value="shortest_branch" /> + <output name="output" file="shortest_branch_basic.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_blobs.gif" /> + <param name="input_datatype" value="gif" /> + <param name="calculate_largest_shortest_path" value="yes" /> + <output name="output" file="largest_shortest_path_basic.tabular" ftype="tabular" /> + </test> + <test> + <!-- TODO: Change this test and the following test to set "show_detailed_info" + to "yes" when the tool has been enhanced to use the future "Display labeled skeletons" + option. See http://fiji.sc/AnalyzeSkeleton. --> + <param name="input" value="skeletonized_blobs.gif" /> + <param name="input_datatype" value="gif" /> + <!-- <param name="show_detailed_info" value="yes" /> --> + <output name="output" file="detailed.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_clown.jpg" /> + <param name="input_datatype" value="jpg" /> + <param name="prune_cycle_method" value="shortest_branch" /> + <param name="calculate_largest_shortest_path" value="yes" /> + <!-- <param name="show_detailed_info" value="yes" /> --> + <output name="output" file="shortest_branch_all_yes.tabular" ftype="tabular" /> + </test> + </tests> + <help> + +.. class:: warningmark + +The underlying AnalyzeSkeleton Fiji plugin works on binary images, so other image types will +automatically be converted to binary before they are analyzed. This step is performed using the +ImageJ2 **Make Binary** command with the following settings: **Iterations:** 1, **Count:** 1, +**Black background:** No, **Pad edges when eroding:** No. If these settings are not appropriate, +first manually convert the image to binary using the **Convert to binary (black and white) with +ImageJ2** tool, which allows you to change them. + +.. class:: warningmark + +The **Skeletonize images** tool produces skeleton images that can be used as input to this tool. +Images that have not been skeletonized will not result in errors, but the results may or may not +be useful. + +**What it does** + +<![CDATA[ +Tags all pixel/voxels in a skeleton image and then counts all its junctions, triple and quadruple +points and branches, and measures their average and maximum length. The voxels are classified into +three different categories depending on their 26 neighbors: + +- **End-point** voxels: if they have less than 2 neighbors. +- **Junction** voxels: if they have more than 2 neighbors. +- **Slab** voxels: if they have exactly 2 neighbors. + +----- + +**Options** + +- **Prune cycle method**: prune the possible loops in the skeleton. +- **Prune ends**: prunes any branch that ends in an end-point. +- **Calculate largest shortest path**: calculate the largest shortest path of each skeleton using the APSP (all pairs shortest path). +]]> + </help> + <citations> + <citation type="doi">10.1002/jemt.20829</citation> + <citation type="doi">10.1038/nmeth.2102</citation> + </citations> +</tool>