Mercurial > repos > imgteam > imagej2_bunwarpj_raw_transform
view imagej2_analyze_particles_binary.py @ 0:95b7592729e2 draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
author | imgteam |
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date | Tue, 17 Sep 2019 17:03:42 -0400 |
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#!/usr/bin/env python import argparse import os import shutil import subprocess import tempfile import imagej2_base_utils parser = argparse.ArgumentParser() parser.add_argument( '--input', dest='input', help='Path to the input file' ) parser.add_argument( '--input_datatype', dest='input_datatype', help='Datatype of the input image' ) parser.add_argument( '--black_background', dest='black_background', help='Black background' ) parser.add_argument( '--size', dest='size', help='Size (pixel^2)' ) parser.add_argument( '--circularity_min', dest='circularity_min', type=float, help='Circularity minimum' ) parser.add_argument( '--circularity_max', dest='circularity_max', type=float, help='Circularity maximum' ) parser.add_argument( '--show', dest='show', help='Show' ) parser.add_argument( '--display_results', dest='display_results', help='Display results' ) parser.add_argument( '--all_results', dest='all_results', help='All results' ) parser.add_argument( '--exclude_edges', dest='exclude_edges', help='Exclude edges' ) parser.add_argument( '--include_holes', dest='include_holes', help='Include holes' ) parser.add_argument( '--jython_script', dest='jython_script', help='Path to the Jython script' ) parser.add_argument( '--results', dest='results', default=None, help='Path to the output results file' ) parser.add_argument( '--output', dest='output', default=None, help='Path to the output image file' ) parser.add_argument( '--output_datatype', dest='output_datatype', default='data', help='Datatype of the output image' ) args = parser.parse_args() tmp_dir = imagej2_base_utils.get_temp_dir() # ImageJ expects valid image file extensions, so the Galaxy .dat extension does not # work for some features. The following creates a symlink with an appropriate file # extension that points to the Galaxy dataset. This symlink is used by ImageJ. tmp_input_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.input, args.input_datatype ) if args.output is None: tmp_output_path = None else: tmp_output_path = imagej2_base_utils.get_temporary_image_path( tmp_dir, args.output_datatype ) # Define command response buffers. tmp_out = tempfile.NamedTemporaryFile().name tmp_stdout = open( tmp_out, 'wb' ) tmp_err = tempfile.NamedTemporaryFile().name tmp_stderr = open( tmp_err, 'wb' ) # Java writes a lot of stuff to stderr, so we'll specify a file for handling actual errors. error_log = tempfile.NamedTemporaryFile( delete=False ).name # Build the command line. cmd = imagej2_base_utils.get_base_command_imagej2( None, jython_script=args.jython_script ) if cmd is None: imagej2_base_utils.stop_err( "ImageJ not found!" ) cmd += ' %s' % error_log cmd += ' %s' % tmp_input_path cmd += ' %s' % args.black_background cmd += ' %s' % args.size cmd += ' %.3f' % args.circularity_min cmd += ' %.3f' % args.circularity_max cmd += ' %s' % args.show cmd += ' %s' % args.display_results cmd += '%s' % imagej2_base_utils.handle_none_type( args.all_results, val_type='str' ) cmd += ' %s' % args.exclude_edges cmd += ' %s' % args.include_holes cmd += '%s' % imagej2_base_utils.handle_none_type( tmp_output_path, val_type='str' ) cmd += ' %s' % args.output_datatype cmd += '%s' % imagej2_base_utils.handle_none_type( args.results, val_type='str' ) # Run the command. proc = subprocess.Popen( args=cmd, stderr=tmp_stderr, stdout=tmp_stdout, shell=True ) rc = proc.wait() # Handle execution errors. if rc != 0: error_message = imagej2_base_utils.get_stderr_exception( tmp_err, tmp_stderr, tmp_out, tmp_stdout ) imagej2_base_utils.stop_err( error_message ) # Handle processing errors. if os.path.getsize( error_log ) > 0: error_message = open( error_log, 'r' ).read() imagej2_base_utils.stop_err( error_message ) if tmp_output_path is not None: # Save the output image. shutil.move( tmp_output_path, args.output ) imagej2_base_utils.cleanup_before_exit( tmp_dir )