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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8f49f3c66b5a1de99ec15e65c2519a56792f1d56
author imgteam
date Wed, 25 Sep 2024 16:04:54 +0000
parents 056e74fbbf6c
children
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1f92b1da556b planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8f49f3c66b5a1de99ec15e65c2519a56792f1d56
imgteam
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1 <tool id="imagej2_enhance_contrast" name="Enhance contrast" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="23.0">
2
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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2 <description>with ImageJ2</description>
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3 <macros>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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4 <import>imagej2_macros.xml</import>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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5 </macros>
2
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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6 <edam_operations>
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7 <edam_operation>operation_3443</edam_operation>
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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8 </edam_operations>
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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9 <xrefs>
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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10 <xref type="bio.tools">imagej</xref>
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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11 <xref type="biii">imagej2</xref>
056e74fbbf6c planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 57a0433defa3cbc37ab34fbb0ebcfaeb680db8d5
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12 </xrefs>
1
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13 <expand macro="fiji_requirements"/>
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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14 <command detect_errors="exit_code"><![CDATA[
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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15 #import os
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16 #set input_sans_ext = $os.path.splitext($os.path.basename($input.file_name))[0]
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1f92b1da556b planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8f49f3c66b5a1de99ec15e65c2519a56792f1d56
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17 #set input_with_ext = './' + '.'.join([input_sans_ext, $input.ext])
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18 ln -s '$input.file_name' '$input_with_ext' &&
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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19 #set output_filename = '.'.join(['output', $input.ext])
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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20 touch '$output_filename' &&
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21 ImageJ --ij2 --headless --debug
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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22 --jython '$__tool_directory__/imagej2_enhance_contrast_jython_script.py'
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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23 '$input_with_ext'
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24 '$equalize_histogram_cond.equalize_histogram'
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25 #if $equalize_histogram_cond.equalize_histogram == 'no':
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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26 $equalize_histogram_cond.saturated_pixels
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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27 '$equalize_histogram_cond.normalize'
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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28 #else:
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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29 'None'
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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30 'no'
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31 #end if
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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32 '$output_filename'
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33 '$input.ext'
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34 && mv '$output_filename' '$output';
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35 ]]></command>
0
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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36 <inputs>
1
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37 <expand macro="param_input"/>
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38 <conditional name="equalize_histogram_cond">
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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39 <param name="equalize_histogram" type="select" label="Equalize histogram?">
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40 <option value="no" selected="True">No</option>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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41 <option value="yes">Yes</option>
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42 </param>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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43 <when value="no">
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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44 <param name="saturated_pixels" type="float" value="0.3" min="0.0" max="100.0" label="Saturated pixels pct" help="Value is percentage of total number of pixels">
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45 <validator type="in_range" min="0" exclude_min="true" max="100"/>
0
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46 </param>
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47 <param name="normalize" type="select" label="Normalize?">
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48 <option value="no" selected="True">No</option>
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49 <option value="yes">Yes</option>
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50 </param>
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51 </when>
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52 <when value="yes"/>
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53 </conditional>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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54 </inputs>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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55 <outputs>
1
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56 <data name="output" format_source="input"/>
0
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57 </outputs>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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58 <tests>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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59 <test>
1
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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60 <param name="input" value="blobs.gif"/>
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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61 <param name="equalize_histogram" value="yes"/>
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62 <output name="output" file="blobs_equalize.gif" compare="image_diff"/>
0
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63 </test>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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64 <test>
1
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65 <param name="input" value="blobs.gif"/>
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66 <param name="saturated_pixels" value="6.2"/>
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67 <output name="output" file="blobs_saturate.gif" compare="image_diff"/>
0
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68 </test>
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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69 <test>
1
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70 <param name="input" value="blobs.gif"/>
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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71 <param name="saturated_pixels" value="13.0"/>
ef3de3e84817 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
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72 <param name="normalize" value="yes"/>
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73 <output name="output" file="blobs_normalize.gif" compare="image_diff"/>
0
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74 </test>
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75 </tests>
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76 <help>
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77
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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78 **What it does**
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79
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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80 <![CDATA[
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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81
edfc597fb180 "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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82 Enhances image contrast by using either normalization (contrast stretching) or histogram
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83 equalization.
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84
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85 Normalization (i.e., contrast stretching) attempts to improve the contrast in an image by
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86 stretching the range of intensity values it contains to span a desired range of values
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87 (e.g. the the full range of pixel values that the image type allows). It differs from the
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88 more sophisticated histogram equalization in that it can only apply a linear scaling function
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89 to the image pixel values. As a result the enhancement is less harsh.
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90
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91 Histogram equalization provides a sophisticated method for modifying the dynamic range and
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92 contrast of an image by altering the image such that its intensity histogram has a desired
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93 shape. Unlike contrast stretching, histogram modeling operators may employ non-linear and
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94 non-monotonic transfer functions to map between pixel intensity values in the input and output
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95 images. Histogram equalization re-assigns the intensity values of pixels in the input image
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96 such that the output image contains a uniform distribution of intensities (i.e. a flat histogram).
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97
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98 - **Equalize histogram** - Enhances the image using histogram equalization. Choosing this option eliminates the "Saturated pixels pct" and "Normalize" options.
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99 - **Saturated pixels pct** - Determines the number of pixels in the image that are allowed to become saturated. Increasing this value increases contrast. This value should be greater than zero to prevent a few outlying pixels from causing the histogram stretch to not work as intended.
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100 - **Normalize** - Recalculates the pixel values of the image so the range is equal to the maximum range for the data type, or 0-1.0 for float images. The maximum range is 0-255 for 8-bit images and 0-65535 for 16-bit images. Normalization of RGB images is not supported, so selecting "yes" is ignored for this image type.
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101
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102 ]]>
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103
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104 </help>
1
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105 <expand macro="fiji_headless_citations"/>
0
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106 </tool>