Mercurial > repos > imgteam > imagej2_enhance_contrast
view imagej2_find_maxima_jython_script.py @ 1:ef3de3e84817 draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
author | imgteam |
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date | Mon, 28 Sep 2020 16:58:37 +0000 |
parents | edfc597fb180 |
children | 056e74fbbf6c |
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import sys from ij import IJ, ImagePlus from ij.plugin.filter import Analyzer, MaximumFinder from ij.process import ImageProcessor # Fiji Jython interpreter implements Python 2.5 which does not # provide support for argparse. error_log = sys.argv[-10] input_file = sys.argv[-9] scale_when_converting = sys.argv[-8] == 'yes' weighted_rgb_conversions = sys.argv[-7] == 'yes' noise_tolerance = int(sys.argv[-6]) output_type = sys.argv[-5] exclude_edge_maxima = sys.argv[-4] == 'yes' light_background = sys.argv[-3] tmp_output_path = sys.argv[-2] output_datatype = sys.argv[-1] # Open the input image file. input_image_plus = IJ.openImage(input_file) # Create a copy of the image. input_image_plus_copy = input_image_plus.duplicate() image_processor_copy = input_image_plus_copy.getProcessor() bit_depth = image_processor_copy.getBitDepth() analyzer = Analyzer(input_image_plus_copy) # Set the conversion options. options = [] # The following 2 options are applicable only to RGB images. if bit_depth == 24: if scale_when_converting: options.append("scale") if weighted_rgb_conversions: options.append("weighted") # Perform conversion - must happen even if no options are set. IJ.run(input_image_plus_copy, "Conversions...", "%s" % " ".join(options)) if output_type in ['List', 'Count']: # W're generating a tabular file for the output. # Set the Find Maxima options. options = ['noise=%d' % noise_tolerance] if output_type.find('_') > 0: output_type_str = 'output=[%s]' % output_type.replace('_', ' ') else: output_type_str = 'output=%s' % output_type options.append(output_type_str) if exclude_edge_maxima: options.append('exclude') if light_background: options.append('light') # Run the command. IJ.run(input_image_plus_copy, "Find Maxima...", "%s" % " ".join(options)) results_table = analyzer.getResultsTable() results_table.saveAs(tmp_output_path) else: # Find the maxima of an image (does not find minima). # LIMITATIONS: With output_type=Segmented_Particles # (watershed segmentation), some segmentation lines # may be improperly placed if local maxima are suppressed # by the tolerance. mf = MaximumFinder() if output_type == 'Single_Points': output_type_param = mf.SINGLE_POINTS elif output_type == 'Maxima_Within_Tolerance': output_type_param = mf.IN_TOLERANCE elif output_type == 'Segmented_Particles': output_type_param = mf.SEGMENTED elif output_type == 'List': output_type_param = mf.LIST elif output_type == 'Count': output_type_param = mf.COUNT # Get a new byteProcessor with a normal (uninverted) LUT where # the marked points are set to 255 (Background 0). Pixels outside # of the roi of the input image_processor_copy are not set. No # output image is created for output types POINT_SELECTION, LIST # and COUNT. In these cases findMaxima returns null. byte_processor = mf.findMaxima(image_processor_copy, noise_tolerance, ImageProcessor.NO_THRESHOLD, output_type_param, exclude_edge_maxima, False) # Invert the image or ROI. byte_processor.invert() if output_type == 'Segmented_Particles' and not light_background: # Invert the values in this image's LUT (indexed color model). byte_processor.invertLut() image_plus = ImagePlus("output", byte_processor) IJ.saveAs(image_plus, output_datatype, tmp_output_path)