Mercurial > repos > imgteam > imagej2_find_edges
annotate imagej2_enhance_contrast_jython_script.py @ 0:8e608a6e44ac draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
| author | imgteam | 
|---|---|
| date | Tue, 17 Sep 2019 16:59:03 -0400 | 
| parents | |
| children | c8dfbf4b899c | 
| rev | line source | 
|---|---|
| 0 
8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 1 import jython_utils | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 2 import sys | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 3 from ij import IJ | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 4 | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 5 # Fiji Jython interpreter implements Python 2.5 which does not | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 6 # provide support for argparse. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 7 error_log = sys.argv[ -7 ] | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 8 input = sys.argv[ -6 ] | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 9 equalize_histogram = jython_utils.asbool( sys.argv[ -5 ] ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 10 saturated_pixels = sys.argv[ -4 ] | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 11 normalize = jython_utils.asbool( sys.argv[ -3 ] ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 12 tmp_output_path = sys.argv[ -2 ] | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 13 output_datatype = sys.argv[ -1 ] | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 14 | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 15 # Open the input image file. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 16 input_image_plus = IJ.openImage( input ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 17 | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 18 # Create a copy of the image. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 19 input_image_plus_copy = input_image_plus.duplicate() | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 20 image_processor_copy = input_image_plus_copy.getProcessor() | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 21 bit_depth = image_processor_copy.getBitDepth() | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 22 | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 23 # Set the options | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 24 options = [] | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 25 # If equalize_histogram, saturated_pixels and normalize are ignored. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 26 if equalize_histogram: | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 27 options.append( 'equalize' ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 28 else: | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 29 if saturated_pixels not in [ None, 'None' ]: | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 30 # Fiji allows only a single decimal place for this value. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 31 options.append( 'saturated=%.3f' % float( saturated_pixels ) ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 32 # Normalization of RGB images is not supported. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 33 if bit_depth != 24 and normalize: | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 34 options.append( 'normalize' ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 35 try: | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 36 # Run the command. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 37 options = "%s" % ' '.join( options ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 38 IJ.run( input_image_plus_copy, "Enhance Contrast...", options ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 39 # Save the ImagePlus object as a new image. | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 40 IJ.saveAs( input_image_plus_copy, output_datatype, tmp_output_path ) | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 41 except Exception, e: | 
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8e608a6e44ac
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 42 jython_utils.handle_error( error_log, str( e ) ) | 
