Mercurial > repos > immport-devteam > fcs_scatterplot
comparison getDensityPlots.xml @ 1:413f3e610295 draft default tip
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/fcs_scatterplot commit 82365bd2b90a783f84f497afbb29b435cd25cf9d"
| author | azomics |
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| date | Thu, 16 Jul 2020 08:00:52 -0400 |
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| 0:164cd5384f1d | 1:413f3e610295 |
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| 1 <tool id="gen_density_plots" name="Generate scatter plots" version="1.0+galaxy0"> | |
| 2 <description>for any given markers in FCS file</description> | |
| 3 <requirements> | |
| 4 <requirement type="package" version="1.10.0">bioconductor-flowdensity</requirement> | |
| 5 </requirements> | |
| 6 <stdio> | |
| 7 <exit_code range="1:9" level="fatal" /> | |
| 8 <exit_code range="10" level="fatal" description="Please provide a comma separated list of channels to plot" /> | |
| 9 <exit_code range="11" level="fatal" description="Please provide numeric values for the list of channels to plot" /> | |
| 10 <exit_code range="12" level="fatal" description="Please provide more than one channel to plot" /> | |
| 11 <exit_code range="13:" level="fatal" /> | |
| 12 </stdio> | |
| 13 <command><![CDATA[ | |
| 14 Rscript --slave --vanilla '$__tool_directory__/getDensityPlots.R' '${input}' '${channels}' '${output}' '${outformat}' | |
| 15 ]]> | |
| 16 </command> | |
| 17 <inputs> | |
| 18 <param format="fcs" name="input" type="data" label="FCS file"/> | |
| 19 <param name="channels" type="text" label="Markers to plot:" value="i.e.:1,3,4" help="By default, will plot FSC vs SSC if the channels are found."/> | |
| 20 <param name="outformat" type="select" label="Output Format" help="PDF will be larger files that may take some time to load."> | |
| 21 <option value="PNG">PNG</option> | |
| 22 <option value="PDF">PDF</option> | |
| 23 </param> | |
| 24 </inputs> | |
| 25 <outputs> | |
| 26 <data format="png" name="output" label="Scatter Plots of ${channels} from ${input.name} in ${outformat}"> | |
| 27 <change_format> | |
| 28 <when input="outformat" value="PDF" format="pdf" /> | |
| 29 </change_format> | |
| 30 </data> | |
| 31 </outputs> | |
| 32 <tests> | |
| 33 <test> | |
| 34 <param name="input" value="testfcs1.fcs"/> | |
| 35 <param name="channels" value="1,3"/> | |
| 36 <param name="outformat" value="PDF"/> | |
| 37 <output name="output" file="graph.pdf" compare="sim_size" delta="1000000"/> | |
| 38 </test> | |
| 39 <test> | |
| 40 <param name="input" value="testfcs1.fcs"/> | |
| 41 <param name="channels" value="i.e.:1,3,4"/> | |
| 42 <param name="outformat" value="PNG"/> | |
| 43 <output name="output" file="graph2.png" compare="sim_size" delta="1000000"/> | |
| 44 </test> | |
| 45 </tests> | |
| 46 <help><![CDATA[ | |
| 47 This tool allows generation of density scatter plots using flowDensity. | |
| 48 | |
| 49 ----- | |
| 50 | |
| 51 **Input files** | |
| 52 | |
| 53 This tool takes valid FCS files as input. | |
| 54 | |
| 55 **Output files** | |
| 56 | |
| 57 This tool generates a scatter plot for each marker combination in a single png file. A pdf file can optionally be generated. | |
| 58 | |
| 59 class:: warningmark | |
| 60 | |
| 61 PDF are larger files that may take some time to load. It might be faster to download the PDF output once generated to open it locally. | |
| 62 | |
| 63 ----- | |
| 64 | |
| 65 **Example** | |
| 66 | |
| 67 *Output*: | |
| 68 | |
| 69 .. image:: ./static/images/flowtools/densityplots.png | |
| 70 ]]> | |
| 71 </help> | |
| 72 <citations> | |
| 73 <citation type="doi">10.1093/bioinformatics/btu677</citation> | |
| 74 <citation type="doi">10.1186/1471-2105-10-106</citation> | |
| 75 </citations> | |
| 76 </tool> |
