Mercurial > repos > immport-devteam > fcs_summary
diff FCSstats.xml @ 1:d2749aa59d20 draft default tip
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/fcs_summary commit 72efaa6d006ddda6c8eed10ec9ba541cbdecf3a8"
author | azomics |
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date | Mon, 22 Jun 2020 17:33:38 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/FCSstats.xml Mon Jun 22 17:33:38 2020 -0400 @@ -0,0 +1,72 @@ +<tool id="get_fcs_stats" name="Get summary" version="1.0+galaxy0"> + <description>of FCS files</description> + <requirements> + <requirement type="package" version="1.42.0">bioconductor-flowcore</requirement> + </requirements> + <stdio> + <exit_code range="2:" level="fatal" description="See stderr for more details." /> + </stdio> + <command><![CDATA[ + Rscript --slave --vanilla '$__tool_directory__/FCSstats.R' '${input}' '${output}' + ]]> + </command> + <inputs> + <param format="fcs" name="input" type="data" label="FCS file"/> + </inputs> + <outputs> + <data format="txt" name="output" label="Summary statisitics of ${input.name}"/> + </outputs> + <tests> + <test> + <param name="input" value="testfcs1.fcs"/> + <output name="output" file="summary.txt" compare="sim_size"/> + </test> + </tests> + <help><![CDATA[ + This tool generates a summary of a FCS file and list of markers. + +----- + +**Input file** + +This tool uses valid FCS files as input. + +**Output file** + +FCS file summary includes number of events, list of markers and parameters, and summary statistics for each. + +----- + +**Example** + +*Output*:: + + events parameters + 20000 6 + + + ========================= + == FCS SUMMARY == + ========================= + FSC-H SSC-H FL1-H FL2-H FL3-H FL4-H + Min. 261 23 0.0 0.0 0.0 0.0 + 1st Qu. 371 120 129.0 153.0 257.0 252.0 + Median 432 196 206.0 237.0 432.0 331.0 + Mean 512 476 284.8 236.7 433.2 372.3 + 3rd Qu. 657 1023 499.0 297.0 628.0 510.0 + Max. 1023 1023 729.0 925.0 1023.0 1023.0 + + + ========================= + == MARKERS IN FCS == + ========================= + channels markers + 1 FSC-H Forward Scatter + 2 SSC-H Side Scatter + 3 FL1-H FITC CD4 + 4 FL2-H PE CD25 + 5 FL3-H PP CD3 + 6 FL4-H APC CD45RA + ]]> + </help> +</tool>