Mercurial > repos > immport-devteam > flowcl
diff getOntology.xml @ 1:f70f75e89890 draft default tip
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/flowcl commit d59d95d2bc6a64eb5c37b8291a7c314754c2067f"
author | azomics |
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date | Thu, 23 Jul 2020 08:50:18 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/getOntology.xml Thu Jul 23 08:50:18 2020 -0400 @@ -0,0 +1,102 @@ +<tool id="get_cell_ontology" name="Explore cell ontology" version="1.0+galaxy0" profile="18.01"> + <description>given a phenotype using flowCL</description> + <requirements> + <requirement type="package" version="2.11.2">jinja2</requirement> + <requirement type="package" version="1.26.0">bioconductor-flowcl</requirement> + </requirements> + <stdio> + <exit_code range="1" level="fatal"/> + <exit_code range="2" level="fatal" description="There was a problem running flowCL. You might want to check your marker names - See stderr for more details." /> + <exit_code range="3:" level="fatal" description="See stderr for more details." /> + </stdio> + <command><![CDATA[ + python '$__tool_directory__/getOntology.py' -o '${html_file}' -d '${html_file.files_path}' -t '$__tool_directory__' -m '${marker1}' -y '${type1}' + #for $mrkrs in $Markers + -m '${mrkrs.markername}' + -y '${mrkrs.markertype}' + #end for + ]]> + </command> + <inputs> + <param name="marker1" type="text" label="First marker"/> + <param name="type1" type="select" label="First marker type"> + <option value="+">+</option> + <option value="-">-</option> + <option value="lo">lo</option> + <option value="lo">low</option> + <option value="lo">dim</option> + <option value="lo">--</option> + <option value="hi">hi</option> + <option value="hi">high</option> + <option value="hi">bri</option> + <option value="hi">bright</option> + <option value="hi">++</option> + </param> + <repeat name="Markers" title="Marker"> + <param name="markername" type="text" label="Add marker"/> + <param name="markertype" type="select" label="Marker type"> + <option value="+">+</option> + <option value="-">-</option> + <option value="lo">lo</option> + <option value="lo">low</option> + <option value="lo">dim</option> + <option value="lo">--</option> + <option value="hi">hi</option> + <option value="hi">high</option> + <option value="hi">bri</option> + <option value="hi">bright</option> + <option value="hi">++</option> + </param> + </repeat> + </inputs> + <outputs> + <data format="html" name="html_file" label="flowCL run"/> + </outputs> + <tests> + <test> + <param name="marker1" value="CD4"/> + <param name="type1" value="+"/> + <output name="html_file" file="out.html" compare="sim_size"> + <extra_files type="file" name="flowCL_res.pdf" value="flowCL_res.pdf" compare="sim_size"/> + <extra_files type="file" name="flowCL_run_summary.txt" value="flowCL_run_summary.txt" compare="sim_size"/> + <extra_files type="file" name="flowCL_table.txt" value="flowCL_table.txt" compare="sim_size"/> + </output> + </test> + </tests> + <help><![CDATA[ + This tool uses flowCL to find the most likely match to a given set a markers. + +----- + +**Input** + +Type in the marker names and select the expression level in the drop-down menu. Marker names need to be in the Cell Ontology for this to work. + +**Output** + +A summary of the ouptut of flowCL is captured in a txt file. For more details, please refer to the flowCL documentation. + +**Graphical output** + +FlowCL generates a plot for the most likely matches to ontology. + +----- + +**Example** + +*Output summary* + +The CL terms hyperlink to their representation in the Immport Cell Ontology browser. + +.. image:: ./static/images/flowtools/flowcl_summary.png + +*Graphical output* + +.. image:: ./static/images/flowtools/flowcl_graph.png + + ]]> + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btu807</citation> + </citations> +</tool>