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author | immport-devteam |
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date | Mon, 27 Feb 2017 12:56:34 -0500 |
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<tool id="get_cell_ontology" name="Explore cell ontology" version="1.0"> <description>given a phenotype using flowCL.</description> <requirements> <requirement type="package" version="2.8">jinja2</requirement> <requirement type="package" version="3.2.3">r</requirement> <requirement type="package" version="1.11.1">bioconductor-flowcl</requirement> </requirements> <stdio> <exit_code range="2" level="fatal" description="There was a problem running flowCL. You might want to check your marker names - See stderr for more details." /> <exit_code range="3:" level="fatal" description="See stderr for more details." /> </stdio> <command><![CDATA[ python $__tool_directory__/getOntology.py -o "${html_file}" -d "${html_file.files_path}" -t $__tool_directory__ -m "${marker1}" -y "${type1}" #for $mrkrs in $Markers -m "${mrkrs.markername}" -y "${mrkrs.markertype}" #end for ]]> </command> <inputs> <param name="marker1" type="text" label="First marker"/> <param name="type1" type="select" label="First marker type"> <option value="+">+</option> <option value="-">-</option> <option value="lo">lo</option> <option value="lo">low</option> <option value="lo">dim</option> <option value="lo">--</option> <option value="hi">hi</option> <option value="hi">high</option> <option value="hi">bri</option> <option value="hi">bright</option> <option value="hi">++</option> </param> <repeat name="Markers" title="Marker"> <param name="markername" type="text" label="Add marker"/> <param name="markertype" type="select" label="Marker type"> <option value="+">+</option> <option value="-">-</option> <option value="lo">lo</option> <option value="lo">low</option> <option value="lo">dim</option> <option value="lo">--</option> <option value="hi">hi</option> <option value="hi">high</option> <option value="hi">bri</option> <option value="hi">bright</option> <option value="hi">++</option> </param> </repeat> </inputs> <outputs> <data format="html" name="html_file" label="flowCL run"/> </outputs> <tests> <test> <param name="marker1" value="CD4"/> <param name="type1" value="+"/> <output name="html_file" file="out.html"> <extra_files type="file" name="flowCL_res.pdf" value="flowCL_res.pdf" compare="contains"/> <extra_files type="file" name="flowCL_run_summary.txt" value="flowCL_run_summary.txt"/> <extra_files type="file" name="flowCL_table.txt" value="flowCL_table.txt"/> </output> </test> </tests> <help><![CDATA[ This tool uses flowCL to find the most likely match to a given set a markers. ----- **Input** Type in the marker names and select the expression level in the drop-down menu. **Output** A summary of the ouptut of flowCL is captured in a txt file. For more details, please refer to the flowCL documentation. **Graphical output** FlowCL generates a plot for the most likely matches to ontology. ----- **Example** *Output summary* The CL terms hyperlink to their representation in the Immport Cell Ontology browser. .. image:: static/images/flowcl_summary.png *Graphical output* .. image:: static/flowtools/flowcl_graph.png ]]> </help> <citations> <citation type="doi">10.1093/bioinformatics/btu807</citation> </citations> </tool>