Mercurial > repos > iuc > abricate

annotate abricate.xml @ 10:c2ef298da409 draft default tip

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/abricate/ commit 6285862fce2b3e298ef0333489392335091b5b0d"
author iuc
date Thu, 15 Apr 2021 18:21:41 +0000
parents b734db305578
children
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1 <tool id="abricate" name="ABRicate" version="@VERSION@" profile="@PROFILE@">
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2 <description>
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3 Mass screening of contigs for antimicrobial and virulence genes
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4 </description>
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5 <macros>
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6 <import>macros.xml</import>
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7 </macros>
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8 <expand macro='xrefs'/>
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9 <expand macro='edam_ontology'/>
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10 <expand macro="requirements" />
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11 <expand macro="version_command" />
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12
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13 <command detect_errors="exit_code"><![CDATA[
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14 #import re
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15 #set $sample_name = re.sub('[^\w\-_\.]', '_', $file_input.element_identifier)
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17 ln -sf '${file_input}' ${sample_name} &&
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18
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19 abricate ${sample_name}
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20 $adv.no_header
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21 #if $adv.min_dna_id
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22 --minid=$adv.min_dna_id
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23 #end if
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24 #if $adv.min_cov
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25 --mincov=$adv.min_cov
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26 #end if
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27 --db=$adv.db
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28 > '$report'
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29 ]]></command>
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30
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31 <inputs>
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32 <param name="file_input" type="data" format="fasta,genbank,embl" label="Input file (Fasta, Genbank or EMBL file)" help="To screen for antibiotic resistant genes, can be a fasta file, a genbank file or an EMBL file." />
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33 <section name="adv" title="Advanced options" expanded="False">
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34 <param argument="--db" type="select" label="Database to use - default is 'resfinder'" help="Option to switch to other AMR/other database">
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35 <option value="argannot">ARG-ANNOT</option>
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36 <option value="card">CARD</option>
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37 <option value="ecoh">EcOH</option>
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38 <option value="ncbi">NCBI Bacterial Antimicrobial Resistance Reference Gene Database</option>
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39 <option value="resfinder" selected="true">Resfinder</option>
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40 <option value="plasmidfinder">PlasmidFinder</option>
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41 <option value="vfdb">VFDB</option>
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42 <option value="megares">megares</option>
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43 <option value="ecoli_vf">Ecoli_VF</option>
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44 </param>
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45 <param name="no_header" argument="--noheader" type="boolean" truevalue="--noheader" falsevalue="" label="Suppress header" help="Suppress output file's column headings" />
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46 <param name="min_dna_id" argument="--minid" type="float" value="80" min="0" max="100" optional="true" label="Minimum DNA %identity" />
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47 <param name="min_cov" argument="--mincov" type="float" value="80" min="0" max="100" optional="true" label="Minimum DNA %coverage" />
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48 </section>
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49 </inputs>
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50
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51 <outputs>
1
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52 <data name="report" format="tabular" label="${tool.name} on ${on_string} report file" />
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53 </outputs>
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54
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55 <tests>
2
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56 <!-- Basic test 0 - will produce no results. -->
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57 <test>
2
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58 <param name="file_input" value="Acetobacter.fna"/>
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59 <output name="report" ftype="tabular" file="output_noresults.txt" />
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60 </test>
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61
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62 <!-- Basic test 1 - will produce results. -->
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63 <test>
2
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64 <param name="file_input" value="MRSA0252.fna"/>
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65 <output name="report" ftype="tabular" file="output_mrsa.txt" compare="contains"/>
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66 </test>
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67
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68 <!-- Advanced test 2 - No header. -->
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69 <test>
2
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70 <param name="file_input" value="MRSA0252.fna"/>
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71 <param name="no_header" value="True"/>
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72 <output name="report" ftype="tabular" file="output_noheader.txt" compare="contains"/>
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73 </test>
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74
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75 <!-- Advanced test 3 - Changed DB to card -->
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76 <test>
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77 <param name="file_input" value="MRSA0252.fna"/>
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78 <param name="db" value="card"/>
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79 <output name="report" ftype="tabular" file="output_db-card.txt" compare="contains"/>
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80 </test>
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81
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82 <!-- Advanced test 4 - Changed DB to megares -->
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83 <test>
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84 <param name="file_input" value="MRSA0252.fna"/>
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85 <param name="db" value="megares"/>
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86 <output name="report" ftype="tabular" file="output_db-megares.txt" compare="contains"/>
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87 </test>
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88
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89 <!-- Advanced test 5 - Min DNA ID 100 -->
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90 <test>
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91 <param name="file_input" value="MRSA0252.fna"/>
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92 <param name="min_dna_id" value="100"/>
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93 <output name="report" ftype="tabular" file="output_minid100.txt" compare="contains"/>
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94 </test>
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95
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96 <!-- Advanced test 6 - Min Coverage 100 -->
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97 <test>
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98 <param name="file_input" value="MRSA0252.fna"/>
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99 <param name="min_cov" value="100"/>
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100 <output name="report" ftype="tabular" file="output_mincov100.txt" compare="contains"/>
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101 </test>
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102
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103 <!-- Filetype test 7 - input a gbk -->
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104 <test>
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105 <param name="file_input" value="test.gbk"/>
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106 <output name="report" ftype="tabular" file="output_gbk.txt" compare="contains"/>
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107 </test>
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108
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109 </tests>
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110
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111 <help><![CDATA[
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112 **What it does**
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113
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114 Given a FASTA contig file or a genbank file, ABRicate will perform a mass screening of contigs and identify the presence of antibiotic resistance genes. The user can choose which database to search from a list of available AMR databases.
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115
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116 **Output**
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117
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118 ABRicate will produce a tab-seperated output file with the following outputs:
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119
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120 +---------------+------------------------------------------------+
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121 | Column | Description |
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122 +===============+================================================+
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123 | FILE | The filename this hit came from |
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124 +---------------+------------------------------------------------+
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125 | SEQUENCE | The sequence in the filename |
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126 +---------------+------------------------------------------------+
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127 | START | Start coordinate in the sequence |
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128 +---------------+------------------------------------------------+
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129 | END | End coordinate |
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130 +---------------+------------------------------------------------+
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131 | GENE | ABR gene |
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132 +---------------+------------------------------------------------+
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133 | COVERAGE | What proportion of the gene is in our sequence |
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134 +---------------+------------------------------------------------+
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135 | COVERAGE_MAP | A visual represenation |
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136 +---------------+------------------------------------------------+
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137 | GAPS | Was there any gaps in the |
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138 | | alignment - possible pseudogene? |
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139 +---------------+------------------------------------------------+
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140 | %COVERAGE | Proportion of gene covered |
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141 +---------------+------------------------------------------------+
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142 | %IDENTITY | Proportion of exact nucleotide matches |
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143 +---------------+------------------------------------------------+
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144 | DATABASE | The database this sequence comes from |
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145 +---------------+------------------------------------------------+
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146 | ACCESSION | The genomic source of the sequence |
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147 +---------------+------------------------------------------------+
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148
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149
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150 **Example Output**
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151
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152 ::
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153
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154 #FILE SEQUENCE START END GENE COVERAGE COVERAGE_MAP GAPS %COVERAGE %IDENTITY DATABASE ACCESSION
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155 6159.fna NC_017338.1 39177 41186 mecA_15 1-2010/2010 =============== 0/0 100.00 100.000 resfinder AB505628
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156 6159.fna NC_017338.1 727191 728356 norA_1 1-1166/1167 =============== 0/0 99.91 92.367 resfinder M97169
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157 6159.fna NC_017339.1 10150 10995 blaZ_32 1-846/846 =============== 0/0 100.00 100.000 resfinder AP004832
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158 ]]></help>
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159
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160 <expand macro="citations"/>
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161 </tool>