Mercurial > repos > iuc > ampligone
changeset 0:d5fc5d888a46 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/ampligone commit 5bd763da003ce033467702f2fb4dca5264e0be43
| author | iuc |
|---|---|
| date | Tue, 29 Jul 2025 10:18:37 +0000 |
| parents | |
| children | |
| files | ampligone.xml test-data/ARTIC-V5.3.2.fasta test-data/SARS-CoV-2-ARTIC-V5.3.2.scheme.bed test-data/SARS-CoV-2-reference.fasta test-data/sars-cov-2.fastq test-data/synthetic.bam test-data/synthetic.bed test-data/synthetic.fasta |
| diffstat | 8 files changed, 1210 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ampligone.xml Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,179 @@ +<tool id="ampligone" name="AmpliGone" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="23.2"> + <description>Find and remove primers from NGS amplicon reads</description> + <macros> + <token name="@TOOL_VERSION@">2.0.1</token> + <token name="@VERSION_SUFFIX@">0</token> + </macros> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">AmpliGone</requirement> + </requirements> + <version_command>ampligone --version</version_command> + <command detect_errors="exit_code"><![CDATA[ + #import re + + #set $inputs_map = { + 'input': 'reads', + 'reference': 'reference', + 'primers': 'primers' + } + + #for $key, $short in $inputs_map.items() + #set $ds = $inputs[$key] + #set $ext = re.sub("sanger", "", $ds.ext) + #set $is_gz = str($ds.ext).endswith('.gz') and $ext != 'bam' + #set $filename = $short + '.' + $ext + + ln -sf '$ds' '$filename' && + #silent $inputs_map[$key] = $filename + + #if $key == 'input' + #set $output_name = 'output.fastq' + ('.gz' if $is_gz else '') + touch '$cleaned' && + ln -sf '$cleaned' $output_name && + #end if + #end for + + #if $inputs.primers.ext == 'fasta' and $inputs.export_primers == 'yes' + #set $primer_filename = 'primers.bed' + touch '$exp_prim' && + ln -sf '$exp_prim' $primer_filename && + #end if + + ## --- Run the tool using clean and predictable filenames --- + ampligone + ##add input files + --input '$inputs_map['input']' + --reference '$inputs_map['reference']' + --primers '$inputs_map['primers']' + + ## Compute options + --threads "\${GALAXY_SLOTS:-2}" + + ##optional arguments + + #if $opt_args.ampli_type.amplicon_type + --amplicon-type $opt_args.ampli_type.amplicon_type + #end if + #if $opt_args.ampli_type.amplicon_type == 'fragmented' + --fragment-lookaround-size $opt_args.ampli_type.fragment_lookaround_size + #end if + #if $opt_args.error_rate + --error-rate $opt_args.error_rate + #end if + + ##output options + #if $inputs.primers.ext == 'fasta' and $inputs.export_primers == 'yes' + --export-primers '$primer_filename' + #end if + + --output '$output_name' + + ]]></command> + <inputs> + <section name="inputs" title="Inputs" expanded="true"> + <param argument="--input" type="data" format="fastqsanger,fastqsanger.gz,bam" label="Input file" help="Input file with reads in FASTQ format."/> + <param argument="--reference" type="data" format="fasta" label="Reference genome" help="Input Reference genome in FASTA format." /> + <param argument="--primers" type="data" format="fasta,bed" label="Used primer sequences" help="Used primer sequences in FASTA or BED format." /> + <param argument="--export-primers" type="boolean" checked="false" truevalue="yes" falsevalue="no" optional="true" label="Output cut primers coordinates" help="Output BED file with found primer coordinates if they are actually cut from the reads."/> + </section> + + <section name="opt_args" title="Optional Arguments" expanded="false"> + <conditional name="ampli_type"> + <param argument="--amplicon-type" type="select" label="Define the amplicon-type" help="Define the amplicon-type, either being 'end-to-end', 'end-to-mid', or 'fragmented'. See the docs for more info."> + <option value="end-to-end" selected="true">end-to-end</option> + <option value="end-to-mid">end-to-mid</option> + <option value="fragmented">fragmented</option> + </param> + <when value="fragmented"> + <param argument="--fragment-lookaround-size" type="integer" value="10" optional="true" label="Fragment lookaround size" help="The number of bases to look around a primer-site to consider it part of a fragment." /> + </when> + <when value="end-to-end"/> + <when value="end-to-mid"/> + </conditional> + <param argument="--error-rate" type="float" min="0" max="1" value="0.1" label="Error rate" help="The maximum allowed error rate for the primer search. Use 0 for exact primer matches." /> + </section> + </inputs> + + <outputs> + <data name="cleaned" format="fastqsanger" label="${tool.name} on ${inputs.input.name} ($on_string): Cleaned reads"/> + <data name="exp_prim" format="bed" label="${tool.name} on ${on_string}: Detected primer coordinates"> + <filter>inputs['primers'].ext == 'fasta' and inputs['export_primers'] is True</filter> + </data> + </outputs> + <tests> + <test expect_num_outputs="1"> + <section name="inputs"> + <param name="input" value="sars-cov-2.fastq"/> + <param name="reference" value="SARS-CoV-2-reference.fasta"/> + <param name="primers" value="SARS-CoV-2-ARTIC-V5.3.2.scheme.bed" /> + </section> + <output name="cleaned"> + <assert_contents> + <has_text text="@SRR30635841.1"/> + <has_text text="@SRR30635841.2"/> + <has_text text="@SRR30635841.3"/> + <has_text text="@SRR30635841.4"/> + <has_text text="@SRR30635841.5"/> + </assert_contents> + </output> + </test> + <test expect_num_outputs="2"> + <section name="inputs"> + <param name="input" value="sars-cov-2.fastq"/> + <param name="reference" value="SARS-CoV-2-reference.fasta"/> + <param name="primers" value="ARTIC-V5.3.2.fasta" /> + <param name="export_primers" value="yes"/> + </section> + <output name="cleaned"> + <assert_contents> + <has_text text="@SRR30635841.1"/> + <has_text text="@SRR30635841.2"/> + <has_text text="@SRR30635841.3"/> + <has_text text="@SRR30635841.4"/> + <has_text text="@SRR30635841.5"/> + </assert_contents> + </output> + <output name="exp_prim"> + <assert_contents> + <has_text text="MN908947.3:6204-6237_LEFT"/> + <has_text text="MN908947.3:25744-25777_RIGHT"/> + </assert_contents> + </output> + </test> + <test expect_num_outputs="1"> + <section name="inputs"> + <param name="input" value="synthetic.bam"/> + <param name="reference" value="synthetic.fasta"/> + <param name="primers" value="synthetic.bed" /> + </section> + <output name="cleaned"> + <assert_contents> + <has_text text="read_number_2_last_120_of_ref"/> + <has_text text="read_number_1_first_120_of_ref"/> + </assert_contents> + </output> + </test> + </tests> + <help><![CDATA[ +**AmpliGone** + +In contrast to a lot of other primer-removal tools, AmpliGone does not actively look for primer sequences within the NGS reads. When providing BED input, reads are trimmed based on primer sequence coordinates in relation to a given reference sequence. Additionally, AmpliGone is able to compensate for, and therefore properly clean, reads that start or end outside of a primer-region as this is a common occurrence in amplicon-based sequencing data. +AmpliGone works with both reads in FASTQ format, as well as aligned data in BAM-format. However, when data is presented in the BAM-format then only read-data (sequence and quality scores) will be used. Other data present in the BAM-format will not be used in this version of AmpliGone. + +Currently, AmpliGone supports Nanopore data and Illumina data. The Illumina platform (NextSeq/MiSeq/HiSeq/other) does not matter. + +It is however important that you know the read-length in relation to the amplicon length. AmpliGone expects this information in the form of an 'amplicon-type'. + +AmpliGone is build and tested with Nanopore and Illumina data (fastq) in mind and supports 'end-to-end', 'end-to-mid' and 'fragmented' amplicons to be cleaned. + + +**More Information** + +- **Official Repository**: https://github.com/RIVM-bioinformatics/AmpliGone +- **Extended User Guide**: https://rivm-bioinformatics.github.io/AmpliGone/@TOOL_VERSION@/ + + ]]></help> + <citations> + <citation type="doi">10.5281/zenodo.7684307</citation> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ARTIC-V5.3.2.fasta Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,384 @@ +>MN908947.3:47-78_LEFT +CTCTTGTAGATCTGTTCTCTAAACGAACTTT +>MN908947.3:419-447_RIGHT +GCTTAGTAGAAGTTGAAAAAGGCGTTTT +>MN908947.3:344-366_LEFT +TCGTACGTGGCTTTGGAGACTC +>MN908947.3:707-732_RIGHT +AGCTTGGCACTGATCCTTATGAAGA +>MN908947.3:638-661_LEFT +AGAACGGTAATAAAGGAGCTGGT +>MN908947.3:1018-1047_RIGHT +CAGACACCTTTTGAAATTAAATTGGCAAA +>MN908947.3:970-995_LEFT +CATGAAATTGCTTGGTACACGGAAC +>MN908947.3:1340-1370_RIGHT +ACTTACCCCAAAATGCTGTTGTTAAAATTT +>MN908947.3:1292-1320_LEFT +TTTGTGGCACTGAGAATTTGACTAAAGA +>MN908947.3:1660-1692_RIGHT +GACTTTAAACTTAATGAAGAGATCGCCATTAT +>MN908947.3:1574-1596_LEFT +GTGTTGTTGGAGAAGGTTCCGA +>MN908947.3:1945-1972_RIGHT +GTTTTACAGAAGGCCGCTATAACAATA +>MN908947.3:1882-1905_LEFT +GCTGCTCGTGTTGTACGATCAAT +>MN908947.3:2259-2284_RIGHT +CACCTGTGCAAAGGAAATTAAGGAG +>MN908947.3:2229-2252_LEFT +TGCTTGTGAAATTGTCGGTGGAC +>MN908947.3:2603-2629_RIGHT +GTACACCAGTTTGTATTAACGGGCTT +>MN908947.3:2533-2563_LEFT +GTCTTGAAAACTGGTGATTTACAACCATTA +>MN908947.3:2900-2933_RIGHT +TCATAAAAACTTTGCAACCAGTATCTGAATTAC +>MN908947.3:2854-2880_LEFT +CTCGGTACAGAAGTAAATGAGTTCGC +>MN908947.3:3233-3254_RIGHT +AACAAGACGGCAGTGAGGACA +>MN908947.3:3184-3213_LEFT +GAGCAAGAAGAAGATTGGTTAGATGATGA +>MN908947.3:3560-3584_RIGHT +TGGGTGGTAGTTGTGTTTTAAGCG +>MN908947.3:3510-3540_LEFT +CATGCAAGTTGAATCTGATGATTACATAGC +>MN908947.3:3883-3913_RIGHT +CCTAAAGAGGAAGTTAAGCCATTTATAACT +>MN908947.3:3791-3824_LEFT +CTGTCTTTGATAAAAATCTCTATGACAAACTTG +>MN908947.3:4147-4180_RIGHT +GTTTTAACTGCTGTGGTTATACCTACTAAAAAG +>MN908947.3:4079-4108_LEFT +GTGACATTGACATCACTTTCTTAAAGAAA +>MN908947.3:4457-4488_RIGHT +TAGTTTCAACTATACAGCGTAAATATAAGGG +>MN908947.3:4403-4425_LEFT +CACATGCAGAAGAAACACGCAA +>MN908947.3:4776-4803_RIGHT +CATCTCACTTGCTGGTTCCTATAAAGA +>MN908947.3:4723-4756_LEFT +AATGGTTATCTTACTTCTTCTTCTAAAACACCT +>MN908947.3:5089-5119_RIGHT +CCTCATAATTCACATGAAGGTAAAACATTT +>MN908947.3:5036-5063_LEFT +GACAACAGTTTGGTCCAACTTATTTGG +>MN908947.3:5398-5429_RIGHT +GCACTTATCTTAGCCTACTGTAATAAGACAG +>MN908947.3:5344-5370_LEFT +GCTCTACAAGATGCTTATTACAGAGC +>MN908947.3:5716-5744_RIGHT +CATGGTACATTTACTTGTGCTAGTGAGT +>MN908947.3:5671-5696_LEFT +CCTTTTGTTATGATGTCAGCACCAC +>MN908947.3:6031-6062_RIGHT +GCAAGCTTCGATAATTTTAAGTTTGTATGTG +>MN908947.3:5891-5923_LEFT +CCATAAAACCAGTTACTTATAAATTGGATGGT +>MN908947.3:6257-6288_RIGHT +CTAATAAAGCCACGTATAAACCAAATACCTG +>MN908947.3:6204-6237_LEFT +GAAAGGAGCTAAATTGTTACATAAACCTATTGT +>MN908947.3:6562-6595_RIGHT +GTAGACAATTCTAGTCTTACTATTAAGAAACCT +>MN908947.3:6515-6542_LEFT +TAAAAATTACAGAAGAGGTTGGCCACA +>MN908947.3:6882-6915_RIGHT +CGGTAAATTTTGTCTAGAGGCTTCATTTAATTA +>MN908947.3:6823-6854_LEFT +AATTCTAGAATTAAAGCATCTATGCCGACTA +>MN908947.3:7199-7229_RIGHT +CATCTTTTAAATGGGATTTAACTGCTTTTG +>MN908947.3:7145-7179_LEFT +GTTTAGATTCTTTAGACACCTATCCTTCTTTAGA +>MN908947.3:7518-7545_RIGHT +AACAAGAGTCGAATGTACAACTATTGT +>MN908947.3:7456-7482_LEFT +GTGCATGTTGTAGACGGTTGTAATTC +>MN908947.3:7819-7850_RIGHT +TCTCATTTTGTTAACTTAGACAACCTGAGAG +>MN908947.3:7768-7797_LEFT +CATCTTTACTTTGATAAAGCTGGTCAAAA +>MN908947.3:8136-8169_RIGHT +GTCCTTAGACAATGTCTTATCTACTTTTATTTC +>MN908947.3:8085-8112_LEFT +AAAACTCAAAACACTAGTTGCAACTGC +>MN908947.3:8468-8498_RIGHT +AGAATAACTTACCTTTTAAGTTGACATGTG +>MN908947.3:8406-8436_LEFT +CGTTAAAGATTTCATGTCATTGTCTGAACA +>MN908947.3:8781-8806_RIGHT +CCAGCGTGGTGGTAGTTATACTAAT +>MN908947.3:8732-8761_LEFT +CAGATACTTGTTTTGCTAACAAACATGCT +>MN908947.3:9107-9129_RIGHT +TACGCCCTGACACACGTTATGT +>MN908947.3:9023-9052_LEFT +CAATTTTTAAAGATGCTTCTGGTAAGCCA +>MN908947.3:9397-9423_RIGHT +GCATCTATAGTAGCTGGTGGTATTGT +>MN908947.3:9299-9324_LEFT +GATCTTTACCAGGAGTTTTCTGTGG +>MN908947.3:9673-9706_RIGHT +CCTTTCTGGATAACAATTGCTTATATCATTTGT +>MN908947.3:9571-9604_LEFT +GGTGTTTATTCTGTTATTTACTTGTACTTGACA +>MN908947.3:9949-9971_RIGHT +GCTGCTTGTTGTCATCTCGCAA +>MN908947.3:9896-9929_LEFT +ATAATAAGTACAAGTATTTTAGTGGAGCAATGG +>MN908947.3:10266-10295_RIGHT +TAATGTTCAACTCAGGGTTATTGGACATT +>MN908947.3:10215-10245_LEFT +TGAAGATTTACTCATTCGTAAGTCTAATCA +>MN908947.3:10587-10615_RIGHT +AGGTAACTTTTATGGACCTTTTGTTGAC +>MN908947.3:10527-10557_LEFT +TTTTTGTTACATGCACCATATGGAATTACC +>MN908947.3:10897-10927_RIGHT +TTGGGTAGTGCTTTATTAGAAGATGAATTT +>MN908947.3:10832-10865_LEFT +CCGTTTTAGATATGTGTGCTTCATTAAAAGAAT +>MN908947.3:11201-11232_RIGHT +CTGTAGCTTATTTTAATATGGTCTATATGCC +>MN908947.3:11152-11181_LEFT +AAACATAAGCATGCATTTCTCTGTTTGTT +>MN908947.3:11514-11536_RIGHT +TGTCATGTTTTTGGCCAGAGGT +>MN908947.3:11463-11494_LEFT +GTGGGCTCTTATAATCTCTGTTACTTCTAAC +>MN908947.3:11832-11863_RIGHT +CACTGTACAGTCTAAAATGTCAGATGTAAAG +>MN908947.3:11785-11811_LEFT +AACATTAAATTGTTGGGTGTTGGTGG +>MN908947.3:12161-12185_RIGHT +AGGCTGTTGCTAATGGTGATTCTG +>MN908947.3:12112-12137_LEFT +TCCCTTCCATCATATGCAGCTTTTG +>MN908947.3:12477-12510_RIGHT +GGTTGTCATACCAGACTATAACACATATAAAAA +>MN908947.3:12419-12444_LEFT +CAAGAGATGGTTGTGTTCCCTTGAA +>MN908947.3:12794-12819_RIGHT +GAGGTAGGTTTGTACTTGCACTGTT +>MN908947.3:12752-12774_LEFT +GCACTGATGACAATGCGTTAGC +>MN908947.3:13121-13146_RIGHT +GTGGGGGACAACCAATCACTAATTG +>MN908947.3:13075-13099_LEFT +GCTTTTGCTGTAGATGCTGCTAAA +>MN908947.3:13458-13480_RIGHT +GTTTTTAAACGGGTTTGCGGTG +>MN908947.3:13415-13435_LEFT +ATCAACTCCGCGAACCCATG +>MN908947.3:13787-13815_RIGHT +TCAACGTCTTACTAAATACACAATGGCA +>MN908947.3:13738-13767_LEFT +ACTTCTTTAAGTTTAGAATAGACGGTGAC +>MN908947.3:14120-14144_RIGHT +AGGTAGTGGAGTTCCTGTTGTAGA +>MN908947.3:14073-14100_LEFT +CTCAATGGTAACTGGTATGATTTCGGT +>MN908947.3:14427-14457_RIGHT +GTGAGAAAAATATTTGTTGATGGTGTTCCA +>MN908947.3:14375-14407_LEFT +CTTTAATGTTTTATTCTCTACAGTGTTCCCAC +>MN908947.3:14745-14775_RIGHT +GAATTAAAACACTTCTTCTTTGCTCAGGAT +>MN908947.3:14700-14725_LEFT +GACTTTGCTGTGTCTAAGGGTTTCT +>MN908947.3:15065-15095_RIGHT +GAATCTTAAGTATGCCATTAGTGCAAAGAA +>MN908947.3:15016-15045_LEFT +CACTTTTCGCATATACAAAACGTAATGTC +>MN908947.3:15386-15416_RIGHT +ACACCGTTTCTATAGATTAGCTAATGAGTG +>MN908947.3:15342-15366_LEFT +TCACTTGTTCTTGCTCGCAAACAT +>MN908947.3:15716-15742_RIGHT +TGACGATGCTGTTGTGTGTTTCAATA +>MN908947.3:15659-15688_LEFT +CTTTGTGAATGAGTTTTACGCATATTTGC +>MN908947.3:16028-16059_RIGHT +TATAGATGCTTACCCACTTACTAAACATCCT +>MN908947.3:15992-16018_LEFT +TGGTACACTTATGATTGAACGGTTCG +>MN908947.3:16386-16409_RIGHT +AATGCTCCAGGTTGTGATGTCAC +>MN908947.3:16285-16311_LEFT +GTGCTTGCATACGTAGACCATTCTTA +>MN908947.3:16650-16679_RIGHT +AAAGCTACTGAGGAGACATTTAAACTGTC +>MN908947.3:16624-16647_LEFT +TCAAGCTTTTTGCAGCAGAAACG +>MN908947.3:17004-17033_RIGHT +AATATCTCAGATGAGTTTTCTAGCAATGT +>MN908947.3:16962-16994_LEFT +CAAGAGCACTATGTTAGAATTACTGGCTTATA +>MN908947.3:17333-17362_RIGHT +GACAGCAGATATAGTTGTCTTTGATGAAA +>MN908947.3:17182-17212_LEFT +CACTATGTGAGAAGGCATTAAAATATTTGC +>MN908947.3:17560-17582_RIGHT +GGCGTTGTCCTGCTGAAATTGT +>MN908947.3:17478-17507_LEFT +GGCACACTAGAACCAGAATATTTCAATTC +>MN908947.3:17859-17886_RIGHT +GACTATGTCATATTCACTCAAACCACT +>MN908947.3:17813-17839_LEFT +GGGACTACCAACTCAAACTGTTGATT +>MN908947.3:18181-18212_RIGHT +ACATGACCTATAGAAGACTCATCTCTATGAT +>MN908947.3:18121-18153_LEFT +GTGTTGACACTAAATTCAAAACTGAAGGTTTA +>MN908947.3:18504-18527_RIGHT +GGACTTCCTTGGAATGTAGTGCG +>MN908947.3:18460-18484_LEFT +CGCCTGGAGATCAATTTAAACACC +>MN908947.3:18835-18860_RIGHT +ATGCACATGTAGCTAGTTGTGATGC +>MN908947.3:18789-18815_LEFT +GGTAACCTACAAAGCAACCATGATCT +>MN908947.3:19170-19195_RIGHT +ACAGATGGTGTATGCCTATTTTGGA +>MN908947.3:19087-19112_LEFT +TCTATGATGCACAGCCTTGTAGTGA +>MN908947.3:19469-19495_RIGHT +CAATTTAGGTGGTGCTGTCTGTAGAC +>MN908947.3:19415-19449_LEFT +AGTGTCAGATATAGATTATGTACCACTAAAGTCT +>MN908947.3:19770-19796_RIGHT +GTTAATGTAGCATTTGAGCTTTGGGC +>MN908947.3:19721-19750_LEFT +AGTTGATGGTGTTGATGTAGAATTGTTTG +>MN908947.3:20091-20121_RIGHT +AAACAAGCTAGTCTTAATGGAGTCACATTA +>MN908947.3:20028-20054_LEFT +GCCCGTAATGGTGTTCTTATTACAGA +>MN908947.3:20408-20441_RIGHT +TGAATTAGAAGATTTTATTCCTATGGACAGTAC +>MN908947.3:20358-20388_LEFT +GGTTTACATCTACTGATTGGACTAGCTAAA +>MN908947.3:20729-20758_RIGHT +GTGTGACCTTCAAAATTATGGTGATAGTG +>MN908947.3:20650-20676_LEFT +AATTACAATCTAGTCAAGCGTGGCAA +>MN908947.3:21018-21051_RIGHT +GCTAATAAATGGGATCTCATTATTAGTGATATG +>MN908947.3:20991-21018_LEFT +ATTGGTGATTGTGCAACTGTACATACA +>MN908947.3:21372-21402_RIGHT +TTGTCTTCCTATTCTTTATTTGACATGAGT +>MN908947.3:21322-21352_LEFT +ATGTCATGCATGCAAATTACATATTTTGGA +>MN908947.3:21696-21722_RIGHT +CTCAGTTTTACATTCAACTCAGGACT +>MN908947.3:21579-21607_LEFT +TTTATTGCCACTAGTCTCTAGTCAGTGT +>MN908947.3:21927-21960_RIGHT +CGCTACTAATGTTGTTATTAAAGTCTGTGAATT +>MN908947.3:21866-21894_LEFT +GAGGCTGGATTTTTGGTACTACTTTAGA +>MN908947.3:22238-22266_RIGHT +TGGTAGATTTGCCAATAGGTATTAACAT +>MN908947.3:22156-22189_LEFT +GGTTATTTTAAAATATATTCTAAGCACACGCCT +>MN908947.3:22517-22547_RIGHT +GAGTCCAACCAACAGAATCTATTGTTAGAT +>MN908947.3:22466-22494_LEFT +CGTTGAAATCCTTCACTGTAGAAAAAGG +>MN908947.3:22839-22866_RIGHT +AGATGATTTTACAGGCTGCGTTATAGC +>MN908947.3:22742-22774_LEFT +ATGTCTATGCAGATTCATTTGTAATTAGAGGT +>MN908947.3:23119-23140_RIGHT +GCACCAGCAACTGTTTGTGGA +>MN908947.3:23078-23109_LEFT +AACCATACAGAGTAGTAGTACTTTCTTTTGA +>MN908947.3:23452-23478_RIGHT +CCTACTTGGCGTGTTTATTCTACAGG +>MN908947.3:23229-23258_LEFT +CAAAAAGTTTCTGCCTTTCCAACAATTTG +>MN908947.3:23609-23631_RIGHT +GGGCACGTAGTGTAGCTAGTCA +>MN908947.3:23563-23589_LEFT +GCAGGTATATGCGCTAGTTATCAGAC +>MN908947.3:23914-23944_RIGHT +GTCAAACAAATTTACAAAACACCACCAATT +>MN908947.3:23823-23853_LEFT +GCAATATGGCAGTTTTTGTACACAATTAAA +>MN908947.3:24209-24231_RIGHT +CTTCTGGTTGGACCTTTGGTGC +>MN908947.3:24160-24189_LEFT +GATGAAATGATTGCTCAATACACTTCTGC +>MN908947.3:24535-24560_RIGHT +CAAATTGATAGGTTGATCACAGGCA +>MN908947.3:24442-24468_LEFT +ACGCTTGTTAAACAACTTAGCTCCAA +>MN908947.3:24815-24839_RIGHT +GAAAAGCACACTTTCCTCGTGAAG +>MN908947.3:24751-24774_LEFT +CATGTGACTTATGTCCCTGCACA +>MN908947.3:25120-25151_RIGHT +AATGAGGTTGCCAAGAATTTAAATGAATCTC +>MN908947.3:25053-25082_LEFT +TGATTTAGGTGACATCTCTGGCATTAATG +>MN908947.3:25423-25452_RIGHT +GAACTGTAACTTTGAAGCAAGGTGAAATC +>MN908947.3:25372-25402_LEFT +CATTACACATAAACGAACTTATGGATTTGT +>MN908947.3:25744-25777_RIGHT +TAAACTTTGTAAGAATAATAATGAGGCTTTGGC +>MN908947.3:25653-25680_LEFT +GTAACAGTTTACTCACACCTTTTGCTC +>MN908947.3:26048-26072_RIGHT +GAGTACAGACACTGGTGTTGAACA +>MN908947.3:26011-26039_LEFT +TCACTTCAGACTATTACCAGCTGTACTC +>MN908947.3:26382-26411_RIGHT +GTTAACGTGAGTCTTGTAAAACCTTCTTT +>MN908947.3:26339-26362_LEFT +CATCCTTACTGCGCTTCGATTGT +>MN908947.3:26730-26756_RIGHT +TTTACAGAATAAATTGGATCACCGGT +>MN908947.3:26593-26621_LEFT +AGGTTTCCTATTCCTTACATGGATTTGT +>MN908947.3:26989-27009_RIGHT +AGGACGCTGTGACATCAAGG +>MN908947.3:26958-26981_LEFT +GTGGACATCTTCGTATTGCTGGA +>MN908947.3:27349-27376_RIGHT +CTCAATTAGATGAAGAGCAACCAATGG +>MN908947.3:27200-27226_LEFT +GATGTTTCATCTCGTTGACTTTCAGG +>MN908947.3:27583-27603_RIGHT +CTTTTGCTTGTCCTGACGGC +>MN908947.3:27530-27558_LEFT +TCATCCTCTAGCTGATAACAAATTTGCA +>MN908947.3:27927-27950_RIGHT +CTGTAGCTGCATTTCACCAAGAA +>MN908947.3:27832-27860_LEFT +TATCTTTTGGTTCTCACTTGAACTGCAA +>MN908947.3:28209-28237_RIGHT +AAGACTTTTTAGAGTATCATGACGTTCG +>MN908947.3:28135-28166_LEFT +TTCCTGTTTACCTTTTACAATTAATTGCCAG +>MN908947.3:28513-28539_RIGHT +GATGACCAAATTGGCTACTACCGAAG +>MN908947.3:28473-28493_LEFT +TCGAGGACAAGGCGTTCCAA +>MN908947.3:28849-28873_RIGHT +AGTTCAAGAAATTCAACTCCAGGC +>MN908947.3:28808-28829_LEFT +GCAGTCAAGCCTCTTCTCGTT +>MN908947.3:29203-29224_RIGHT +GCTTCAGCGTTCTTCGGAATG +>MN908947.3:29159-29183_LEFT +CTGATTACAAACATTGGCCGCAAA +>MN908947.3:29538-29559_RIGHT +TGCAGACCACACAAGGCAGAT +>MN908947.3:29462-29486_LEFT +CTGCAGATTTGGATGATTTCTCCA +>MN908947.3:29840-29873_RIGHT +GTGATTTTAATAGCTTCTTAGGAGAATGACAAA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/SARS-CoV-2-ARTIC-V5.3.2.scheme.bed Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,192 @@ +MN908947.3 47 78 SARS-CoV-2_400_1_LEFT_1 1 + +MN908947.3 419 447 SARS-CoV-2_400_1_RIGHT_1 1 - +MN908947.3 344 366 SARS-CoV-2_400_2_LEFT_0 2 + +MN908947.3 707 732 SARS-CoV-2_400_2_RIGHT_0 2 - +MN908947.3 638 661 SARS-CoV-2_400_3_LEFT_1 1 + +MN908947.3 1018 1047 SARS-CoV-2_400_3_RIGHT_0 1 - +MN908947.3 970 995 SARS-CoV-2_400_4_LEFT_0 2 + +MN908947.3 1340 1370 SARS-CoV-2_400_4_RIGHT_0 2 - +MN908947.3 1292 1320 SARS-CoV-2_400_5_LEFT_0 1 + +MN908947.3 1660 1692 SARS-CoV-2_400_5_RIGHT_0 1 - +MN908947.3 1574 1596 SARS-CoV-2_400_6_LEFT_1 2 + +MN908947.3 1945 1972 SARS-CoV-2_400_6_RIGHT_1 2 - +MN908947.3 1882 1905 SARS-CoV-2_400_7_LEFT_2 1 + +MN908947.3 2259 2284 SARS-CoV-2_400_7_RIGHT_2 1 - +MN908947.3 2229 2252 SARS-CoV-2_400_8_LEFT_0 2 + +MN908947.3 2603 2629 SARS-CoV-2_400_8_RIGHT_0 2 - +MN908947.3 2533 2563 SARS-CoV-2_400_9_LEFT_0 1 + +MN908947.3 2900 2933 SARS-CoV-2_400_9_RIGHT_0 1 - +MN908947.3 2854 2880 SARS-CoV-2_400_10_LEFT_0 2 + +MN908947.3 3233 3254 SARS-CoV-2_400_10_RIGHT_0 2 - +MN908947.3 3184 3213 SARS-CoV-2_400_11_LEFT_0 1 + +MN908947.3 3560 3584 SARS-CoV-2_400_11_RIGHT_0 1 - +MN908947.3 3510 3540 SARS-CoV-2_400_12_LEFT_0 2 + +MN908947.3 3883 3913 SARS-CoV-2_400_12_RIGHT_0 2 - +MN908947.3 3791 3824 SARS-CoV-2_400_13_LEFT_0 1 + +MN908947.3 4147 4180 SARS-CoV-2_400_13_RIGHT_0 1 - +MN908947.3 4079 4108 SARS-CoV-2_400_14_LEFT_0 2 + +MN908947.3 4457 4488 SARS-CoV-2_400_14_RIGHT_0 2 - +MN908947.3 4403 4425 SARS-CoV-2_400_15_LEFT_0 1 + +MN908947.3 4776 4803 SARS-CoV-2_400_15_RIGHT_0 1 - +MN908947.3 4723 4756 SARS-CoV-2_400_16_LEFT_0 2 + +MN908947.3 5089 5119 SARS-CoV-2_400_16_RIGHT_0 2 - +MN908947.3 5036 5063 SARS-CoV-2_400_17_LEFT_0 1 + +MN908947.3 5398 5429 SARS-CoV-2_400_17_RIGHT_0 1 - +MN908947.3 5344 5370 SARS-CoV-2_400_18_LEFT_0 2 + +MN908947.3 5716 5744 SARS-CoV-2_400_18_RIGHT_0 2 - +MN908947.3 5671 5696 SARS-CoV-2_400_19_LEFT_0 1 + +MN908947.3 6031 6062 SARS-CoV-2_400_19_RIGHT_0 1 - +MN908947.3 5891 5923 SARS-CoV-2_400_20_LEFT_0 2 + +MN908947.3 6257 6288 SARS-CoV-2_400_20_RIGHT_0 2 - +MN908947.3 6204 6237 SARS-CoV-2_400_21_LEFT_0 1 + +MN908947.3 6562 6595 SARS-CoV-2_400_21_RIGHT_0 1 - +MN908947.3 6515 6542 SARS-CoV-2_400_22_LEFT_0 2 + +MN908947.3 6882 6915 SARS-CoV-2_400_22_RIGHT_0 2 - +MN908947.3 6823 6854 SARS-CoV-2_400_23_LEFT_0 1 + +MN908947.3 7199 7229 SARS-CoV-2_400_23_RIGHT_0 1 - +MN908947.3 7145 7179 SARS-CoV-2_400_24_LEFT_0 2 + +MN908947.3 7518 7545 SARS-CoV-2_400_24_RIGHT_0 2 - +MN908947.3 7456 7482 SARS-CoV-2_400_25_LEFT_0 1 + +MN908947.3 7819 7850 SARS-CoV-2_400_25_RIGHT_0 1 - +MN908947.3 7768 7797 SARS-CoV-2_400_26_LEFT_0 2 + +MN908947.3 8136 8169 SARS-CoV-2_400_26_RIGHT_0 2 - +MN908947.3 8085 8112 SARS-CoV-2_400_27_LEFT_0 1 + +MN908947.3 8468 8498 SARS-CoV-2_400_27_RIGHT_0 1 - +MN908947.3 8406 8436 SARS-CoV-2_400_28_LEFT_0 2 + +MN908947.3 8781 8806 SARS-CoV-2_400_28_RIGHT_0 2 - +MN908947.3 8732 8761 SARS-CoV-2_400_29_LEFT_0 1 + +MN908947.3 9107 9129 SARS-CoV-2_400_29_RIGHT_0 1 - +MN908947.3 9023 9052 SARS-CoV-2_400_30_LEFT_0 2 + +MN908947.3 9397 9423 SARS-CoV-2_400_30_RIGHT_0 2 - +MN908947.3 9299 9324 SARS-CoV-2_400_31_LEFT_1 1 + +MN908947.3 9673 9706 SARS-CoV-2_400_31_RIGHT_0 1 - +MN908947.3 9571 9604 SARS-CoV-2_400_32_LEFT_0 2 + +MN908947.3 9949 9971 SARS-CoV-2_400_32_RIGHT_0 2 - +MN908947.3 9896 9929 SARS-CoV-2_400_33_LEFT_0 1 + +MN908947.3 10266 10295 SARS-CoV-2_400_33_RIGHT_0 1 - +MN908947.3 10215 10245 SARS-CoV-2_400_34_LEFT_0 2 + +MN908947.3 10587 10615 SARS-CoV-2_400_34_RIGHT_0 2 - +MN908947.3 10527 10557 SARS-CoV-2_400_35_LEFT_0 1 + +MN908947.3 10897 10927 SARS-CoV-2_400_35_RIGHT_0 1 - +MN908947.3 10832 10865 SARS-CoV-2_400_36_LEFT_0 2 + +MN908947.3 11201 11232 SARS-CoV-2_400_36_RIGHT_0 2 - +MN908947.3 11152 11181 SARS-CoV-2_400_37_LEFT_0 1 + +MN908947.3 11514 11536 SARS-CoV-2_400_37_RIGHT_0 1 - +MN908947.3 11463 11494 SARS-CoV-2_400_38_LEFT_0 2 + +MN908947.3 11832 11863 SARS-CoV-2_400_38_RIGHT_0 2 - +MN908947.3 11785 11811 SARS-CoV-2_400_39_LEFT_0 1 + +MN908947.3 12161 12185 SARS-CoV-2_400_39_RIGHT_0 1 - +MN908947.3 12112 12137 SARS-CoV-2_400_40_LEFT_0 2 + +MN908947.3 12477 12510 SARS-CoV-2_400_40_RIGHT_0 2 - +MN908947.3 12419 12444 SARS-CoV-2_400_41_LEFT_0 1 + +MN908947.3 12794 12819 SARS-CoV-2_400_41_RIGHT_0 1 - +MN908947.3 12752 12774 SARS-CoV-2_400_42_LEFT_0 2 + +MN908947.3 13121 13146 SARS-CoV-2_400_42_RIGHT_0 2 - +MN908947.3 13075 13099 SARS-CoV-2_400_43_LEFT_0 1 + +MN908947.3 13458 13480 SARS-CoV-2_400_43_RIGHT_0 1 - +MN908947.3 13415 13435 SARS-CoV-2_400_44_LEFT_0 2 + +MN908947.3 13787 13815 SARS-CoV-2_400_44_RIGHT_0 2 - +MN908947.3 13738 13767 SARS-CoV-2_400_45_LEFT_0 1 + +MN908947.3 14120 14144 SARS-CoV-2_400_45_RIGHT_0 1 - +MN908947.3 14073 14100 SARS-CoV-2_400_46_LEFT_0 2 + +MN908947.3 14427 14457 SARS-CoV-2_400_46_RIGHT_0 2 - +MN908947.3 14375 14407 SARS-CoV-2_400_47_LEFT_0 1 + +MN908947.3 14745 14775 SARS-CoV-2_400_47_RIGHT_0 1 - +MN908947.3 14700 14725 SARS-CoV-2_400_48_LEFT_0 2 + +MN908947.3 15065 15095 SARS-CoV-2_400_48_RIGHT_0 2 - +MN908947.3 15016 15045 SARS-CoV-2_400_49_LEFT_0 1 + +MN908947.3 15386 15416 SARS-CoV-2_400_49_RIGHT_0 1 - +MN908947.3 15342 15366 SARS-CoV-2_400_50_LEFT_0 2 + +MN908947.3 15716 15742 SARS-CoV-2_400_50_RIGHT_0 2 - +MN908947.3 15659 15688 SARS-CoV-2_400_51_LEFT_0 1 + +MN908947.3 16028 16059 SARS-CoV-2_400_51_RIGHT_0 1 - +MN908947.3 15992 16018 SARS-CoV-2_400_52_LEFT_2 2 + +MN908947.3 16386 16409 SARS-CoV-2_400_52_RIGHT_2 2 - +MN908947.3 16285 16311 SARS-CoV-2_400_53_LEFT_0 1 + +MN908947.3 16650 16679 SARS-CoV-2_400_53_RIGHT_0 1 - +MN908947.3 16624 16647 SARS-CoV-2_400_54_LEFT_1 2 + +MN908947.3 17004 17033 SARS-CoV-2_400_54_RIGHT_1 2 - +MN908947.3 16962 16994 SARS-CoV-2_400_55_LEFT_1 1 + +MN908947.3 17333 17362 SARS-CoV-2_400_55_RIGHT_1 1 - +MN908947.3 17182 17212 SARS-CoV-2_400_56_LEFT_0 2 + +MN908947.3 17560 17582 SARS-CoV-2_400_56_RIGHT_0 2 - +MN908947.3 17478 17507 SARS-CoV-2_400_57_LEFT_0 1 + +MN908947.3 17859 17886 SARS-CoV-2_400_57_RIGHT_0 1 - +MN908947.3 17813 17839 SARS-CoV-2_400_58_LEFT_0 2 + +MN908947.3 18181 18212 SARS-CoV-2_400_58_RIGHT_0 2 - +MN908947.3 18121 18153 SARS-CoV-2_400_59_LEFT_0 1 + +MN908947.3 18504 18527 SARS-CoV-2_400_59_RIGHT_0 1 - +MN908947.3 18460 18484 SARS-CoV-2_400_60_LEFT_0 2 + +MN908947.3 18835 18860 SARS-CoV-2_400_60_RIGHT_0 2 - +MN908947.3 18789 18815 SARS-CoV-2_400_61_LEFT_0 1 + +MN908947.3 19170 19195 SARS-CoV-2_400_61_RIGHT_0 1 - +MN908947.3 19087 19112 SARS-CoV-2_400_62_LEFT_2 2 + +MN908947.3 19469 19495 SARS-CoV-2_400_62_RIGHT_0 2 - +MN908947.3 19415 19449 SARS-CoV-2_400_63_LEFT_0 1 + +MN908947.3 19770 19796 SARS-CoV-2_400_63_RIGHT_0 1 - +MN908947.3 19721 19750 SARS-CoV-2_400_64_LEFT_0 2 + +MN908947.3 20091 20121 SARS-CoV-2_400_64_RIGHT_0 2 - +MN908947.3 20028 20054 SARS-CoV-2_400_65_LEFT_0 1 + +MN908947.3 20408 20441 SARS-CoV-2_400_65_RIGHT_0 1 - +MN908947.3 20358 20388 SARS-CoV-2_400_66_LEFT_0 2 + +MN908947.3 20729 20758 SARS-CoV-2_400_66_RIGHT_0 2 - +MN908947.3 20650 20676 SARS-CoV-2_400_67_LEFT_1 1 + +MN908947.3 21018 21051 SARS-CoV-2_400_67_RIGHT_1 1 - +MN908947.3 20991 21018 SARS-CoV-2_400_68_LEFT_0 2 + +MN908947.3 21372 21402 SARS-CoV-2_400_68_RIGHT_0 2 - +MN908947.3 21322 21352 SARS-CoV-2_400_69_LEFT_0 1 + +MN908947.3 21696 21722 SARS-CoV-2_400_69_RIGHT_0 1 - +MN908947.3 21579 21607 SARS-CoV-2_400_70_LEFT_0 2 + +MN908947.3 21927 21960 SARS-CoV-2_400_70_RIGHT_0 2 - +MN908947.3 21866 21894 SARS-CoV-2_400_71_LEFT_0 1 + +MN908947.3 22238 22266 SARS-CoV-2_400_71_RIGHT_0 1 - +MN908947.3 22156 22189 SARS-CoV-2_400_72_LEFT_0 2 + +MN908947.3 22517 22547 SARS-CoV-2_400_72_RIGHT_0 2 - +MN908947.3 22466 22494 SARS-CoV-2_400_73_LEFT_0 1 + +MN908947.3 22839 22866 SARS-CoV-2_400_73_RIGHT_0 1 - +MN908947.3 22742 22774 SARS-CoV-2_400_74_LEFT_0 2 + +MN908947.3 23119 23140 SARS-CoV-2_400_74_RIGHT_0 2 - +MN908947.3 23078 23109 SARS-CoV-2_400_75_LEFT_1 1 + +MN908947.3 23452 23478 SARS-CoV-2_400_75_RIGHT_1 1 - +MN908947.3 23229 23258 SARS-CoV-2_400_76_LEFT_0 2 + +MN908947.3 23609 23631 SARS-CoV-2_400_76_RIGHT_0 2 - +MN908947.3 23563 23589 SARS-CoV-2_400_77_LEFT_0 1 + +MN908947.3 23914 23944 SARS-CoV-2_400_77_RIGHT_0 1 - +MN908947.3 23823 23853 SARS-CoV-2_400_78_LEFT_0 2 + +MN908947.3 24209 24231 SARS-CoV-2_400_78_RIGHT_0 2 - +MN908947.3 24160 24189 SARS-CoV-2_400_79_LEFT_0 1 + +MN908947.3 24535 24560 SARS-CoV-2_400_79_RIGHT_0 1 - +MN908947.3 24442 24468 SARS-CoV-2_400_80_LEFT_0 2 + +MN908947.3 24815 24839 SARS-CoV-2_400_80_RIGHT_0 2 - +MN908947.3 24751 24774 SARS-CoV-2_400_81_LEFT_0 1 + +MN908947.3 25120 25151 SARS-CoV-2_400_81_RIGHT_0 1 - +MN908947.3 25053 25082 SARS-CoV-2_400_82_LEFT_0 2 + +MN908947.3 25423 25452 SARS-CoV-2_400_82_RIGHT_0 2 - +MN908947.3 25372 25402 SARS-CoV-2_400_83_LEFT_0 1 + +MN908947.3 25744 25777 SARS-CoV-2_400_83_RIGHT_0 1 - +MN908947.3 25653 25680 SARS-CoV-2_400_84_LEFT_2 2 + +MN908947.3 26048 26072 SARS-CoV-2_400_84_RIGHT_2 2 - +MN908947.3 26011 26039 SARS-CoV-2_400_85_LEFT_0 1 + +MN908947.3 26382 26411 SARS-CoV-2_400_85_RIGHT_0 1 - +MN908947.3 26339 26362 SARS-CoV-2_400_86_LEFT_0 2 + +MN908947.3 26730 26756 SARS-CoV-2_400_86_RIGHT_0 2 - +MN908947.3 26593 26621 SARS-CoV-2_400_87_LEFT_1 1 + +MN908947.3 26989 27009 SARS-CoV-2_400_87_RIGHT_1 1 - +MN908947.3 26958 26981 SARS-CoV-2_400_88_LEFT_2 2 + +MN908947.3 27349 27376 SARS-CoV-2_400_88_RIGHT_2 2 - +MN908947.3 27200 27226 SARS-CoV-2_400_89_LEFT_2 1 + +MN908947.3 27583 27603 SARS-CoV-2_400_89_RIGHT_0 1 - +MN908947.3 27530 27558 SARS-CoV-2_400_90_LEFT_0 2 + +MN908947.3 27927 27950 SARS-CoV-2_400_90_RIGHT_0 2 - +MN908947.3 27832 27860 SARS-CoV-2_400_91_LEFT_0 1 + +MN908947.3 28209 28237 SARS-CoV-2_400_91_RIGHT_0 1 - +MN908947.3 28135 28166 SARS-CoV-2_400_92_LEFT_0 2 + +MN908947.3 28513 28539 SARS-CoV-2_400_92_RIGHT_0 2 - +MN908947.3 28473 28493 SARS-CoV-2_400_93_LEFT_0 1 + +MN908947.3 28849 28873 SARS-CoV-2_400_93_RIGHT_0 1 - +MN908947.3 28808 28829 SARS-CoV-2_400_94_LEFT_0 2 + +MN908947.3 29203 29224 SARS-CoV-2_400_94_RIGHT_0 2 - +MN908947.3 29159 29183 SARS-CoV-2_400_95_LEFT_0 1 + +MN908947.3 29538 29559 SARS-CoV-2_400_95_RIGHT_0 1 - +MN908947.3 29462 29486 SARS-CoV-2_400_96_LEFT_1 2 + +MN908947.3 29840 29873 SARS-CoV-2_400_96_RIGHT_0 2 -
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/SARS-CoV-2-reference.fasta Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,429 @@ +>MN908947.3 Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome +ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAA +CGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAAC +TAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTG +TTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTC +CCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTAC +GTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGG +CTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGAT +GCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTC +GTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCT +TCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTA +GGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTG +TTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGG +CCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTG +TCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTG +CTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAA +ATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAA +CCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCAC +CAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCA +GACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACT +ACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAG +GACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCG +CACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCA +CGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACA +ACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGA +GATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGAT +TATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAG +GTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCG +TGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCC +GCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTG +ATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTG +GCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTT +AAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAA +TTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGT +AAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTA +GGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC +TACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTT +AACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAA +GCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGT +ACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAA +GGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTT +GATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAA +ATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACC +ACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAA +TTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAG +AAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT +TGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAA +CAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTC +AACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTT +AAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACA +GTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTA +CTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAG +TTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGT +GAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTAT +TATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAA +TGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGT +GAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTA +AACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAAC +TCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCA +GATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTG +ATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAAT +GCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAAT +GGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTA +TTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGC +AGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAA +TATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAA +CAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTA +TGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTT +TCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAG +AACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACA +ACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCAC +CTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTA +AGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACA +ACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGT +AAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTG +ATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAA +TGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAA +ATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTA +ACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAAT +GAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGT +GGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAAT +TTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTC +ACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGT +GAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAG +ACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAG +TTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAG +TTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAAC +CATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAA +CCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGT +GATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAAC +CTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTG +TCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGA +ATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGA +AAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAA +TAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTT +ACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTG +CTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTAC +AACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTA +TTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAG +CAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAA +TTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTAC +TCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAG +GCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCT +TAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAA +TGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCT +ATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTC +TTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATC +TTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTT +GTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAG +GTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGT +GATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAA +GACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCA +TCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGAC +AACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAAT +GTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACT +AGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTT +AATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTG +AACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGT +TGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTT +ACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTG +GTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGAT +ATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAG +AATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAG +CACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTT +TGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAA +ATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTA +ACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCC +ATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGC +ACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACAC +CATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTT +TAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTAT +GAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACC +TTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATC +AGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCA +GGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTG +GTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTA +CTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTC +CTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTT +ACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTT +CACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGG +TTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTG +CGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTAC +GCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGC +TACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTC +TTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCC +ATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTT +GATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAG +ATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGG +ACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAG +TTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTT +ACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGG +TTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCAT +GCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTA +CGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTT +TCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTA +ACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTG +CTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGA +TGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA +ATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTC +AATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTC +TGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCC +ACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATA +TGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACT +AATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTG +ACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCT +CTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTG +TGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTC +TTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTG +GTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAA +GAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTA +GCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAAC +TCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAA +AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTA +GACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTA +GTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA +TTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCA +GCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTG +AGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAA +TGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACA +ACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACAT +TTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAG +TGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCT +GCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTA +CACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACT +TGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATC +TATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTAT +ACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCT +ACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGAT +GCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGT +GTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGG +TGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTA +AAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAG +TCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCA +GTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCA +CAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAA +ATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTT +GTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTC +CAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCA +ACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGAC +ACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATG +ATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTT +AAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAA +GATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTG +TAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGT +TGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTA +AAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATG +ACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGG +ACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAG +CTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGT +ATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTC +AGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTAT +GACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTC +AGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAG +ACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCT +AACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGAC +TTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCC +TACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTC +TCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAG +GAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAG +TGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTT +AGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATA +GATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACC +AGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTC +ACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC +AACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGC +ATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTAT +GCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTA +TGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATAC +AATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCC +GGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTA +TAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATA +CATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGAT +AACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTG +TTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTT +ATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTAT +GTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATT +GTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAA +TACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGT +GATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTG +AGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCT +TTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACT +AAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACC +GAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATT +AAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATC +TCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGG +GACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGT +GTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGAT +AAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACAT +TAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGA +AATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTAC +ATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATT +TCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCC +TGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCA +GCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCAC +AAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTA +TAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGC +TCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTA +ATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTT +GCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTC +TTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACA +CTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACT +CATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAA +GAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTG +TTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTA +TGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAA +CACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAA +GTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATC +TATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTT +TCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTA +TGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCA +TGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTT +AAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAA +AGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAA +CCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGT +GACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTG +TATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAG +AGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCAC +ACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTC +CATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTAT +AACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCT +TATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGA +ACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGG +ACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTA +GAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTA +AACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGA +CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAA +CCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTAT +TTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCC +CAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAG +AAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTA +AACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATT +AGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTA +CTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTA +CAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTT +ATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTG +ACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAA +AATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCT +ATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTC +GCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTA +TACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTAC +GGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGAT +TGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAA +ATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCT +AGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATG +GGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTG +GATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTG +GAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTA +AGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAG +GTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAA +CAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCA +ATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCA +GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATG +TCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGC +TTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC +CTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCAT +TTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGC +GAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTC +AAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTA +TTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTAT +TAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCA +GGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATA +ATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTT +GAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATT +GTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTG +TTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATC +ATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTAT +GCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTG +ATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTC +TAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGA +GATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACT +TTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACT +TTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAAC +AAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTC +TGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGA +GATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAAC +CAGGTTGCTGTTCTTTATCAGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTA +CTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGC +TGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACT +CAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTG +GTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTAC +CACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCA +ACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAA +TAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACC +AATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCA +TTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATT +GCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACC +TTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGG +ACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTG +GAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAA +AATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCA +CAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATA +TCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAG +TTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCT +ACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTA +TGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAA +GAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTT +TCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACA +CATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACC +TGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTA +GGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTG +CCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCC +ATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGT +ATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACG +ACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGA +ATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTC +GCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCT +TGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGT +GTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTG +GCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAAT +AATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTT +CTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTA +CTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATG +GGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCA +ACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGC +CTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAAT +TTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTAC +TCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTAT +TCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGT +GAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGAT +CTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGA +TTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTC +CTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTA +AGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAAT +AAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTC +ATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTC +TCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGT +GATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAA +GAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTG +ACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAG +CAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTAC +TATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATA +AACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAAC +CAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGA +GCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA +TACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAAT +TTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACT +GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTT +ATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTG +CTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAA +GATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTG +TAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCC +GTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAA +TTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCT +GTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGA +AGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTG +ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAG +TAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACT +GCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC +CAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGG +TGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCT +GGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAA +AAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAAC +ATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGT +AGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCA +ATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGG +TAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGG +CAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCC +AAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACA +ATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACG +TGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGC +TGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGC +TGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGAT +TTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATG +CAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTT +GTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCT +TTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTAC +GATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAAT +TTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAAAAAAAAAAAAAAAAAA +AAAAAAAAAAAAA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/sars-cov-2.fastq Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,20 @@ +@SRR30635841.1 VL00553:3:AACM5FTM5:1:1101:26525:1000 length=301 +AATTCACAGACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAATGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGATATAACATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAATAAACACCACGTGTGAAAG ++SRR30635841.1 VL00553:3:AACM5FTM5:1:1101:26525:1000 length=301 +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCC*C5C*CCCCCCCCCC5CC +@SRR30635841.2 VL00553:3:AACM5FTM5:1:1101:34648:1133 length=301 +GAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGGTAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCA ++SRR30635841.2 VL00553:3:AACM5FTM5:1:1101:34648:1133 length=301 +CCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCC5CCCCCCCCCCCCCCCCCC5CCCCC*C5CCCCC* +@SRR30635841.3 VL00553:3:AACM5FTM5:1:1101:54190:1133 length=301 +TCATTGCTAGAAAACTCATCTGAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTGGTCTAGGTTTACCAACTTCCCATG ++SRR30635841.3 VL00553:3:AACM5FTM5:1:1101:54190:1133 length=301 +5CCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC*CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC*CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCC**CCCCCC5C +@SRR30635841.4 VL00553:3:AACM5FTM5:1:1101:54606:1133 length=301 +TGTGGTTTGAGTGAATATGACATAGTCATATTCTGAGCCCTGTGATGAATCAACAGTTTGAGTTGGTAGTCCCAAAATCTTTGAGGCTACAGCATTCTGTGAATTATAAGGTGAAATAAAGACAGCTTTTCTCCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCCTGTTAATTGCAGATGAAACATCATGCGTGATAACACCCTTATAAAACATTTTAAAGCATTGAGCTGATTTGTCTTTATGTGCTTTAAGCTTATTATCATAAACCAAAGCACTCACAGTG ++SRR30635841.4 VL00553:3:AACM5FTM5:1:1101:54606:1133 length=301 +5C5CCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCC*CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCCCCCCCCCCCCCCCC*CCCCCC*CCCCCCC5CCCCCCCCC +@SRR30635841.5 VL00553:3:AACM5FTM5:1:1101:51596:1151 length=301 +GCCAAAGCCTCATTATTATTCTTACAAAGTTTATACTCTGCAAGAAGTAGACTAAAGCATAAAGATAGAGAAAAGGGGCTTCAAGGCCAGCAGCAACGAGCAAAAGGTGTGAGTAAACTGTTACAAACAACAACAGCAAGTTGCAAACAAAGTGAACACCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGAGTTATGATTTTGGAAGCGCTCTGAAAAACAGCAAGAAGTGCAACGCCAACAATAAGCCATCCGAAAGGGAGTGAGGCTTGTATCGGTATCGTTGCAGTAGCGCGAAC ++SRR30635841.5 VL00553:3:AACM5FTM5:1:1101:51596:1151 length=301 +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5CCCCC5CCCCCCCCCCCCCCCCCCC*CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC*5CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5 \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/synthetic.bed Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,4 @@ +homemade_reference 0 10 SARS-CoV-2_400_1_LEFT_1 1 + +homemade_reference 110 120 SARS-CoV-2_400_1_RIGHT_1 1 - +homemade_reference 80 90 SARS-CoV-2_400_2_LEFT_1 1 + +homemade_reference 190 200 SARS-CoV-2_400_2_RIGHT_1 1 - \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/synthetic.fasta Tue Jul 29 10:18:37 2025 +0000 @@ -0,0 +1,2 @@ +>homemade_reference +GGAAATTCATTCTAGGGAGTGACGTGGACCCCGGATTGATACAGGATCACATGTAGAAAAGGTAGTCGGACAAGTTACCGCTACCCTCGACCTCGTGGGGCCTACACCTGACCAGGAGCCGCACTGACAGGACCACGCTTCATCATAACTTTGGCGGCTGGGCAACGGATTTAATGGTACATAACTCATCATTCTACGTA \ No newline at end of file