Mercurial > repos > iuc > ampvis2_load
annotate load.xml @ 7:7db90993aa92 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit d92935972ac18906154fadf42bd0c008e3c437e2
author | iuc |
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date | Fri, 25 Oct 2024 14:15:29 +0000 |
parents | 576dd33588bf |
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rev | line source |
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0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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1 <tool id="ampvis2_load" name="ampvis2 load" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@" license="MIT"> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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2 <description></description> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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3 <macros> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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4 <import>macros.xml</import> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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5 </macros> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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6 <expand macro="header"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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7 <command detect_errors="exit_code"><![CDATA[ |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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8 #if $otutable.is_of_type("biom1") or $otutable.is_of_type("biom2") |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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9 ln -s '$otutable' otutable.biom && |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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10 #else if not $otutable.is_of_type("phyloseq") |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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diff
changeset
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11 ## asv/otu column can not be specified so set the needed name |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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12 ## if empty https://github.com/KasperSkytte/ampvis2/issues/166 |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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13 ## also done in taxonomy.tsv |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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14 #if $asv_otu_col_empty |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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15 sed -e '1 s/^\t/ASV\t/' '$otutable' > otutable.tsv && |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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16 #else |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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17 ln -s '$otutable' otutable.tsv && |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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18 #end if |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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19 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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20 #if $taxonomy |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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21 #if $asv_otu_col_empty |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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22 sed -e '1 s/^\t/ASV\t/' '$taxonomy' > taxonomy.tsv && |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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23 #else |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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24 ln -s '$taxonomy' taxonomy.tsv && |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
diff
changeset
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25 #end if |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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26 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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27 Rscript '$rscript' |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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28 ]]></command> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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29 <configfiles> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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30 <configfile name="rscript"><![CDATA[ |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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31 library(ampvis2, quietly = TRUE) |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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32 library(readr, quietly = TRUE) |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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33 ## 'manually' load metadata treating all columns as character |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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changeset
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34 ## giving colClasses to amp_load seems not possible |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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35 ## - check.names=F: leave empty column names empty .. fixed below |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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36 #if $metadata |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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37 metadata <- read.table("$metadata", header = TRUE, sep = "\t", colClasses = "character", check.names=F) |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
2
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38 ## we do not require the metadata to have a 1st column named "SampleID", |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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diff
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39 ## but it should not be empty |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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40 if(colnames(metadata)[1] == ""){ |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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41 colnames(metadata)[1] <- "SampleID" |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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42 } |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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43 if(exists("SampleID", where = metadata)){ |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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44 rownames(metadata) <- metadata[["SampleID"]] |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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45 }else{ |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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46 rownames(metadata) <- metadata[[1]] |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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47 } |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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48 #end if |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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49 |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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50 #if $otutable.is_of_type("phyloseq") |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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51 otutable <- readRDS("$otutable") |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
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52 print(class(otutable)) |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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53 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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54 data <- amp_load( |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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diff
changeset
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55 #if $otutable.is_of_type("phyloseq") |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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56 otutable = otutable, |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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diff
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57 #else if $otutable.is_of_type("biom1") or $otutable.is_of_type("biom2") |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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58 otutable = "otutable.biom", |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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59 #else |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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60 otutable = "otutable.tsv", |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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61 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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62 #if $metadata |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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63 metadata = metadata, |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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64 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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65 #if $taxonomy |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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66 taxonomy = "taxonomy.tsv", |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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67 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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68 #if $fasta |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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69 fasta = "$fasta", |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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70 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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71 #if $tree |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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72 tree = "$tree", |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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73 #end if |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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74 pruneSingletons = $pruneSingletons |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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75 ) |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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76 |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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77 #if $asv_sequences |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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78 library(ape, quietly = TRUE) |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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79 |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents:
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80 seq <- as.DNAbin(strsplit(rownames(data\$abund), "")) |
932d7573a561
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81 names(seq) <- paste0("ASV", seq_along(seq)) |
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82 data\$refseq <- seq |
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83 data <- matchOTUs(data, seq) |
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84 #end if |
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85 |
0
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86 ## try to guess column types with plyr::type.convert |
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87 #if $guess_column_types |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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88 data\$metadata <- readr::type_convert(data\$metadata, guess_integer=TRUE) |
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89 #end if |
3
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90 |
0
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91 saveRDS(data, "$ampvis") |
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92 ## write metadata list for biom input or if metadata is given |
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93 #if "metadata" in $write_lists |
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94 @SAVE_METADATA_LIST@ |
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95 #end if |
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96 |
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97 #if "tax" in $write_lists |
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98 @SAVE_TAX_LIST@ |
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99 #end if |
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100 ## print overview of the data to stdout |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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101 data |
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102 ]]></configfile> |
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103 </configfiles> |
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104 <inputs> |
3
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105 <param argument="otutable" type="data" format="phyloseq,dada2_sequencetable,tabular,biom1,biom2" label="OTU table"/> |
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106 <param name="asv_otu_col_empty" type="boolean" checked="false" label="OTU/ASV column has empty header" help="By default ampvis2 expects a column named ASV or OTU containing the ASV or OTU identifiers. By checking this a column with an empty header will be used (as produced by dada2)."/> |
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107 <param name="asv_sequences" type="boolean" checked="false" label="ASV identifiers are the ASV sequences" help="By checking this the identifiers will be renamed to ASV1, ASV2, etc and the sequences will be stored in the ampvis2 object." /> |
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108 <param argument="metadata" type="data" format="tabular,tsv" optional="true" label="Sample metadata"> |
0
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109 <validator type="expression" message="Table must have at least 1 column"><![CDATA[value.metadata.columns > 0]]></validator> |
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110 </param> |
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111 <param name="guess_column_types" type="boolean" checked="true" label="Guess metadata column types" help="See help"/> |
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112 <param argument="taxonomy" type="data" format="tabular" optional="true" label="Taxonomy table"/> |
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113 <param argument="fasta" type="data" format="fasta" optional="true" label="Fasta file"/> |
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114 <param argument="tree" type="data" format="newick" optional="true" label="Phylogenetic tree"/> |
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115 <param argument="pruneSingletons" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="false" label="Remove singleton OTUs"/> |
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116 <param name="write_lists" type="select" optional="true" multiple="true" label="Output list data sets" help="Needed by most downstream tools. Select if the inputs contain taxonomic / metadata information."> |
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117 <option value="tax" selected="true">Taxonomy list</option> |
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118 <option value="metadata" selected="true">Metadata list</option> |
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119 </param> |
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120 </inputs> |
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121 <outputs> |
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122 <data name="ampvis" format="ampvis2"/> |
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123 <data name="metadata_list_out" format="tabular" label="${tool.name} on ${on_string}: metadata list"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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124 <filter>write_lists and "metadata" in write_lists</filter> |
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125 </data> |
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126 <data name="taxonomy_list_out" format="tabular" label="${tool.name} on ${on_string}: taxonomy list"> |
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127 <filter>write_lists and "tax" in write_lists</filter> |
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128 </data> |
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129 </outputs> |
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130 <tests> |
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131 <!-- load otu table + metadata + taxonomy --> |
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132 <test expect_num_outputs="3"> |
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133 <param name="otutable" value="AalborgWWTPs.otu.csv"/> |
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134 <param name="metadata" value="AalborgWWTPs.tsv" ftype="tsv"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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135 <param name="taxonomy" value="AalborgWWTPs.tax"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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136 <output name="ampvis" value="AalborgWWTPs.rds" ftype="ampvis2" compare="sim_size"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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137 <output name="metadata_list_out" value="AalborgWWTPs-metadata.list"/> |
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138 <output name="taxonomy_list_out" value="AalborgWWTPs-taxonomy.list"/> |
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139 <assert_stdout> |
3
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140 <has_text text="ampvis2 object with 3 elements."/> |
0
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141 <has_text text="575.79"/> |
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142 <has_text text="SampleID, Plant, Date, Year, Period"/> |
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143 <has_text text="200(100%) 194(97%) 177(88.5%) 170(85%) 152(76%) 113(56.5%) 2(1%)"/> |
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144 </assert_stdout> |
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145 </test> |
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146 <!-- load otu table + metadata + taxonomy + tree + fasta --> |
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147 <test expect_num_outputs="3"> |
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148 <param name="otutable" value="AalborgWWTPs.otu.csv"/> |
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149 <param name="metadata" value="AalborgWWTPs.tsv" ftype="tsv"/> |
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150 <param name="taxonomy" value="AalborgWWTPs.tax"/> |
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151 <param name="fasta" value="AalborgWWTPs.fa" ftype="fasta"/> |
474bbc45ddd9
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152 <param name="tree" value="AalborgWWTPs.nwk" ftype="newick"/> |
474bbc45ddd9
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153 <output name="ampvis" value="AalborgWWTPs-complete.rds" ftype="ampvis2" compare="sim_size"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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154 <output name="metadata_list_out" value="AalborgWWTPs-metadata.list"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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155 <output name="taxonomy_list_out" value="AalborgWWTPs-taxonomy.list"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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156 <assert_stdout> |
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157 <has_text text="ampvis2 object with 5 elements."/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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158 <has_text text="575.79"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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159 <has_text text="SampleID, Plant, Date, Year, Period"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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160 <has_text text="200(100%) 194(97%) 177(88.5%) 170(85%) 152(76%) 113(56.5%) 2(1%)"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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161 </assert_stdout> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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162 </test> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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163 <!-- test biom 1/2 input (taken from https://github.com/biocore/biom-format/tree/master/examples) |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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164 metadata seems not to be loaded from a biom file https://github.com/MadsAlbertsen/ampvis2/issues/129 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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165 taxonomy is loaded from all but 1 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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166 --> |
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iuc
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167 <test expect_num_outputs="1"> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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168 <param name="otutable" value="rich-dense.biom" ftype="biom1"/> |
3
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169 <param name="write_lists" value=""/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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170 <output name="ampvis" ftype="ampvis2"> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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171 <assert_contents> |
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iuc
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172 <has_size value="748" delta="2"/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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173 </assert_contents> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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174 </output> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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175 <assert_stdout> |
3
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iuc
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176 <has_text text="ampvis2 object with 3 elements."/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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177 <has_text text="4.5"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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178 <has_text text="SampleID, BarcodeSequence, LinkerPrimerSequence, BODY_SITE, Description"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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179 <has_text text="5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 1(20%)"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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180 </assert_stdout> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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181 </test> |
3
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iuc
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182 <test expect_num_outputs="1"> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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183 <param name="otutable" value="rich-sparse.biom" ftype="biom1"/> |
3
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
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184 <param name="write_lists" value=""/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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185 <output name="ampvis" ftype="ampvis2"> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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186 <assert_contents> |
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576dd33588bf
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iuc
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187 <has_size value="751" delta="2"/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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188 </assert_contents> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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189 </output> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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190 <assert_stdout> |
3
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iuc
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191 <has_text text="ampvis2 object with 3 elements."/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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192 <has_text text="4.5"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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193 <has_text text="SampleID, BarcodeSequence, LinkerPrimerSequence, BODY_SITE, Description"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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194 <has_text text="5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 1(20%)"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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195 </assert_stdout> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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196 </test> |
3
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197 <!-- input file seems to miss metadata check that no metadata & taxonomy is loaded (ampvis2 adds dummy metadata) --> |
932d7573a561
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198 <test expect_num_outputs="1"> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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199 <param name="otutable" value="min_sparse_otu_table_hdf5.biom" ftype="biom2"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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200 <output name="ampvis" ftype="ampvis2"> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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201 <assert_contents> |
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iuc
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|
202 <has_size value="395" delta="2"/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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203 </assert_contents> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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204 </output> |
3
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iuc
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205 <param name="write_lists" value=""/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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206 <assert_stdout> |
3
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iuc
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207 <has_text text="ampvis2 object with 3 elements."/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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208 <has_text text="4.5"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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209 <has_text text="SampleID, DummyVariable"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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210 <has_text text="0(0%) 0(0%) 0(0%) 0(0%) 0(0%) 0(0%) 0(0%)"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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211 </assert_stdout> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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212 </test> |
3
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213 <test expect_num_outputs="1"> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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214 <param name="otutable" value="rich_sparse_otu_table_hdf5.biom" ftype="biom2"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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215 <output name="ampvis" ftype="ampvis2"> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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216 <assert_contents> |
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iuc
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217 <has_size value="753" delta="2"/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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218 </assert_contents> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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219 </output> |
3
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220 <param name="write_lists" value=""/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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221 <assert_stdout> |
3
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iuc
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222 <has_text text="ampvis2 object with 3 elements."/> |
0
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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223 <has_text text="4.5"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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224 <has_text text="SampleID, BODY_SITE, BarcodeSequence, Description, LinkerPrimerSequence"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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225 <has_text text="5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 1(20%)"/> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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226 </assert_stdout> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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227 </test> |
3
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228 <!-- load dada2 ASV table + metadata + taxonomy --> |
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229 <test expect_num_outputs="3"> |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
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230 <param name="otutable" value="dada2-removeBimeraDenovo.tab" ftype="dada2_sequencetable"/> |
932d7573a561
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
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231 <param name="metadata" value="dada2-metadata.tsv" ftype="tsv"/> |
932d7573a561
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iuc
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232 <param name="taxonomy" value="dada2-assignTaxonomy.tabular"/> |
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233 <param name="asv_otu_col_empty" value="true"/> |
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234 <param name="asv_sequences" value="true"/> |
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235 <output name="ampvis" ftype="ampvis2"> |
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236 <assert_contents> |
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237 <has_size min="100" delta="2"/> |
3
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238 </assert_contents> |
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239 </output> |
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240 <output name="metadata_list_out"> |
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241 <assert_contents> |
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242 <has_n_lines n="23"/> |
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243 <has_n_columns n="4"/> |
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244 <has_text text="Sample"/> |
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245 </assert_contents> |
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246 </output> |
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247 <output name="taxonomy_list_out"> |
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248 <assert_contents> |
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249 <has_n_lines n="370"/> |
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250 <has_n_columns n="2"/> |
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251 <has_line line="Bacteria	Kingdom"/> |
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252 </assert_contents> |
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253 </output> |
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254 <assert_stdout> |
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255 <has_text text="ampvis2 object with 4 elements."/> <!-- this also has fasta, i.e. 4 --> |
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256 <has_text text="6212.45"/> |
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257 <has_text text="Sample, time"/> |
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258 <has_text text="232(100%) 232(100%) 232(100%) 231(99.57%) 209(90.09%) 127(54.74%)"/> |
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259 </assert_stdout> |
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260 </test> |
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261 <!-- load data from phyloseq --> |
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262 <test expect_num_outputs="3"> |
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263 <param name="otutable" value="output.phyloseq" ftype="phyloseq"/> |
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264 <output name="ampvis" ftype="ampvis2"> |
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265 <assert_contents> |
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266 <has_size min="100"/> |
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267 </assert_contents> |
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268 </output> |
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269 <output name="metadata_list_out"> |
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270 <assert_contents> |
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271 <has_n_lines n="6"/> |
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272 <has_n_columns n="4"/> |
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273 <has_text text="SampleID"/> |
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274 </assert_contents> |
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275 </output> |
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276 <output name="taxonomy_list_out"> |
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277 <assert_contents> |
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278 <has_n_lines n="147"/> |
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279 <has_n_columns n="2"/> |
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280 <has_line line="Bacteria	Kingdom"/> |
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281 </assert_contents> |
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282 </output> |
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283 <assert_stdout> |
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284 <has_text text="ampvis2 object with 4 elements."/> <!-- this also has fasta, i.e. 4 --> |
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285 <has_text text="SampleID, Property, Number"/> |
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286 <has_text text="64(100%) 64(100%) 64(100%) 64(100%) 62(96.88%) 56(87.5%) 0(0%)"/> |
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287 </assert_stdout> |
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288 </test> |
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289 </tests> |
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290 <help><![CDATA[ |
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291 |
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292 What it does |
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293 ============ |
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294 |
3
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295 This tool reads an OTU or ASV table and corresponding sample metadata, and returns |
0
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296 a RDS data set for use in all ampvis2 tools. It is therefore required to load |
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297 data with this tool before any other ampvis2 tools can be used. |
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298 |
2
8d77d277996e
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299 The Galaxy tool calls the `amp_load <https://kasperskytte.github.io/ampvis2/reference/amp_load.html>`_ |
0
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300 function of the ampvis2 package. This function validates and corrects the |
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301 provided data frames in different ways to make it suitable for the rest of the |
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302 ampvis2 tools. It is important that the provided data sets match the |
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303 requirements as described in the following to work properly. |
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304 |
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305 Input |
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306 ===== |
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307 |
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308 **The OTU-table** |
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309 |
3
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310 contains information about the OTU/ASVs, their read counts in each sample, and |
0
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311 optionally their assigned taxonomy. The OTU table can be given as |
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312 |
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313 - Tabular data set |
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314 - BIOM version (1 and 2) |
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315 |
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316 Metadata and taxonomy in the tabular or BIOM files that are given via the |
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317 ``OTU table`` parameter can is overwritten if by data presented via the |
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318 ``Sample metadata`` or ``Taxonomy table`` parameters. |
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319 |
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320 If given in tabular format the provided OTU-table must be a table with the |
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321 following requirements: |
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322 |
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323 - The rows are OTU IDs and the columns are samples. |
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324 - The OTU IDs are by default expected to be in a column called "OTU", "ASV", or "#OTU ID". |
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325 For data using an empty header for the OTU/ASV colum enable the option *OTU/ASV column has empty header* |
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326 (this allows to process data as produced e.g. by dada2). |
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327 - The column names of the table are the sample IDs, exactly matching those in |
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328 the metadata |
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329 - The last 7 columns are optionally the corresponding taxonomy assigned to the |
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330 OTUs, named "Kingdom", "Phylum", "Class", "Order", "Family", "Genus", "Species". |
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331 |
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332 If the ASV IDs are actually the ASV Sequences then enabling |
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333 *ASV identifiers are the ASV sequences* will rename the identifiers to ASV1, ASV2,... |
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334 (and save the sequences in the ampvis2 object). |
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335 |
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336 Generally avoid special characters and spaces in row- and column names. |
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337 |
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338 The OTU table can also contain the taxonomic information in additional columns: |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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339 Kingdom, Phylum, Class, Order, Family, Genus. |
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340 |
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341 Check `here <https://biom-format.org/>`_ for information on the BIOM formats. |
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342 |
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343 **The metadata** |
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344 |
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345 contains additional information about the samples, for example where each sample |
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346 was taken, date, pH, treatment etc, which is used to compare and group the |
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347 samples during analysis. The amount of information in the metadata is unlimited, |
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348 it can contain any number of columns (variables), however there are a few |
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349 requirements: |
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350 |
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351 - The sample IDs must be in the first column. The sample IDs must match exactly |
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352 to those in the OTU-table. Any unmatched samples between the otutable and |
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353 metadata will be removed with a warning. |
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354 - Generally avoid special characters and spaces in row- and column names. |
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355 |
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356 By default the data types of metadata columns are guessed with |
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357 ``readr::type_convert``. The guessed column types can be seen in the last (4th) |
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358 column of the ``metadata list`` output and also stdout of the tool. Guessing of |
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359 data types can be disabled using the parameter ``Guess metadata column types``. |
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360 If disabled matadata from separate tabular input is treated as character data, |
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361 and if loaded from biom files that data is used as is. Metadata types can be set |
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362 manually using the tool ``ampvis2: set metadata`` |
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363 |
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364 Dates should be given in the format ``YYYY-MM-DD`` (Y: year, M: month, D: day). |
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365 |
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366 In addition to the RDS data set a metadata (resp. taxonomy) list data set is returned |
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367 if metadata (resp. taxonomic information) is given to this tool. It contains |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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368 restructured metadata (taxonomic information) that is used in downstream ampvis2 |
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369 Galaxy tools in order to select metadata / metadata values (resp. taxonomic levels). |
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370 |
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371 **Taxonomy** |
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372 |
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373 is a tabular data set with 7 columns and one row per ASV/OTU: |
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374 |
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375 - the 1st column is identical to the 1st column of the OTU table parameter |
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376 - the remaining columns contain data for Kingdom, Phylum, Class, Order, Family, Genus |
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377 |
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378 Note that the taxonomic information can also be embedded in the OTU table. |
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379 |
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380 **Tree** |
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381 |
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382 a tree with branch lengths in Newick format. |
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383 |
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384 This is needed / usefull only if the data is used as input of: ``ampvis2: |
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385 ordination plot`` for ordination methods NNDS / MMDS with (un)weighted UniFrac |
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386 distances. Note that the loaded tree is also filtered by the ``ampvis2: subset |
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387 ...`` tools. |
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388 |
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389 **Fasta** |
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390 |
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391 a fasta file containing the sequences of the OTUs. Note that this information is |
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392 only used in ``ampvis2: export fasta``. If the OTU table is modified by |
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393 ``ampvis2: mergereplicates`` or the ``ampvis2: subset ...`` tools this might be |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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394 useful to obtain a filtered list of sequences. |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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395 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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396 |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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397 Output |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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398 ====== |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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399 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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400 **RDS** |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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401 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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402 The main output of the tool is an RDS data set that contains the R representation of |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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403 the ampvis2 object containing the provided data (OTU table, metadata, taxonomy, |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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404 phylogenetic tree, and fasta). |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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405 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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406 **List files** |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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407 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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408 Summarize the metadata and taxonomy information: |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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409 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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410 - the taxonomy list file lists all taxa in a 1 column tabular data set |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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411 - the metadata list file lists the Metadata variables (column 1), and the corresponding |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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412 available metadata values (column 2), if the variable is the SampleID (column 3), and |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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413 the data type of the corresponding metadata variable (column 4) |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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414 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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415 These files are auxilliary files that are needed in downstream ``ampvis2`` Galaxy tools |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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416 to allow selecting metadata and taxonomy. They are not passed to the underlying R functions. |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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417 |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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418 Note that, if the no taxonomy (or metadata) is given then the underlying ``ampvis2`` R |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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419 function adds dummy taxonomy (resp. metadata). In this case the output of the list datasets |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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420 can be disabled with the ``Output list data sets`` parameter. |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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421 ]]></help> |
474bbc45ddd9
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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422 <expand macro="citations"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb30e4b8d695e096753fe7cb5056b118b25a87fd
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423 </tool> |