annotate load.xml @ 3:932d7573a561 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
author iuc
date Mon, 26 Feb 2024 07:53:42 +0000
parents 8d77d277996e
children 576dd33588bf
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1 <tool id="ampvis2_load" name="ampvis2 load" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@" license="MIT">
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2 <description></description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="header"/>
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7 <command detect_errors="exit_code"><![CDATA[
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8 #if $otutable.is_of_type("biom1") or $otutable.is_of_type("biom2")
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9 ln -s '$otutable' otutable.biom &&
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10 #else if not $otutable.is_of_type("phyloseq")
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11 ## asv/otu column can not be specified so set the needed name
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12 ## if empty https://github.com/KasperSkytte/ampvis2/issues/166
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13 ## also done in taxonomy.tsv
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14 #if $asv_otu_col_empty
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15 sed -e '1 s/^\t/ASV\t/' '$otutable' > otutable.tsv &&
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16 #else
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17 ln -s '$otutable' otutable.tsv &&
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18 #end if
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19 #end if
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20 #if $taxonomy
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21 #if $asv_otu_col_empty
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22 sed -e '1 s/^\t/ASV\t/' '$taxonomy' > taxonomy.tsv &&
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23 #else
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24 ln -s '$taxonomy' taxonomy.tsv &&
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25 #end if
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26 #end if
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27 Rscript '$rscript'
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28 ]]></command>
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29 <configfiles>
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30 <configfile name="rscript"><![CDATA[
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31 library(ampvis2, quietly = TRUE)
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32 library(readr, quietly = TRUE)
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33 ## 'manually' load metadata treating all columns as character
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34 ## giving colClasses to amp_load seems not possible
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35 ## - check.names=F: leave empty column names empty .. fixed below
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36 #if $metadata
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37 metadata <- read.table("$metadata", header = TRUE, sep = "\t", colClasses = "character", check.names=F)
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38 ## we do not require the metadata to have a 1st column named "SampleID",
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39 ## but it should not be empty
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40 if(colnames(metadata)[1] == ""){
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41 colnames(metadata)[1] <- "SampleID"
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42 }
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43 if(exists("SampleID", where = metadata)){
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44 rownames(metadata) <- metadata[["SampleID"]]
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45 }else{
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46 rownames(metadata) <- metadata[[1]]
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47 }
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48 #end if
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49
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50 #if $otutable.is_of_type("phyloseq")
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51 otutable <- readRDS("$otutable")
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52 print(class(otutable))
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53 #end if
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54 data <- amp_load(
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55 #if $otutable.is_of_type("phyloseq")
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56 otutable = otutable,
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57 #else if $otutable.is_of_type("biom1") or $otutable.is_of_type("biom2")
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58 otutable = "otutable.biom",
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59 #else
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60 otutable = "otutable.tsv",
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61 #end if
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62 #if $metadata
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63 metadata = metadata,
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64 #end if
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65 #if $taxonomy
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66 taxonomy = "taxonomy.tsv",
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67 #end if
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68 #if $fasta
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69 fasta = "$fasta",
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70 #end if
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71 #if $tree
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72 tree = "$tree",
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73 #end if
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74 pruneSingletons = $pruneSingletons
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75 )
3
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76
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77 #if $asv_sequences
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78 library(ape, quietly = TRUE)
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79
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80 seq <- as.DNAbin(strsplit(rownames(data\$abund), ""))
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81 names(seq) <- paste0("ASV", seq_along(seq))
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82 data\$refseq <- seq
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83 data <- matchOTUs(data, seq)
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84 #end if
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85
0
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86 ## try to guess column types with plyr::type.convert
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87 #if $guess_column_types
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88 data\$metadata <- readr::type_convert(data\$metadata, guess_integer=TRUE)
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89 #end if
3
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90
0
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91 saveRDS(data, "$ampvis")
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92 ## write metadata list for biom input or if metadata is given
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93 #if "metadata" in $write_lists
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94 @SAVE_METADATA_LIST@
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95 #end if
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96
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97 #if "tax" in $write_lists
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98 @SAVE_TAX_LIST@
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99 #end if
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100 ## print overview of the data to stdout
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101 data
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102 ]]></configfile>
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103 </configfiles>
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104 <inputs>
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105 <param argument="otutable" type="data" format="phyloseq,dada2_sequencetable,tabular,biom1,biom2" label="OTU table"/>
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106 <param name="asv_otu_col_empty" type="boolean" checked="false" label="OTU/ASV column has empty header" help="By default ampvis2 expects a column named ASV or OTU containing the ASV or OTU identifiers. By checking this a column with an empty header will be used (as produced by dada2)."/>
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107 <param name="asv_sequences" type="boolean" checked="false" label="ASV identifiers are the ASV sequences" help="By checking this the identifiers will be renamed to ASV1, ASV2, etc and the sequences will be stored in the ampvis2 object." />
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108 <param argument="metadata" type="data" format="tabular,tsv" optional="true" label="Sample metadata">
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109 <validator type="expression" message="Table must have at least 1 column"><![CDATA[value.metadata.columns > 0]]></validator>
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110 </param>
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111 <param name="guess_column_types" type="boolean" checked="true" label="Guess metadata column types" help="See help"/>
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112 <param argument="taxonomy" type="data" format="tabular" optional="true" label="Taxonomy table"/>
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113 <param argument="fasta" type="data" format="fasta" optional="true" label="Fasta file"/>
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114 <param argument="tree" type="data" format="newick" optional="true" label="Phylogenetic tree"/>
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115 <param argument="pruneSingletons" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="false" label="Remove singleton OTUs"/>
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116 <param name="write_lists" type="select" optional="true" multiple="true" label="Output list data sets" help="Needed by most downstream tools. Select if the inputs contain taxonomic / metadata information.">
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117 <option value="tax" selected="true">Taxonomy list</option>
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118 <option value="metadata" selected="true">Metadata list</option>
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119 </param>
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120 </inputs>
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121 <outputs>
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122 <data name="ampvis" format="ampvis2"/>
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123 <data name="metadata_list_out" format="tabular" label="${tool.name} on ${on_string}: metadata list">
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124 <filter>write_lists and "metadata" in write_lists</filter>
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125 </data>
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126 <data name="taxonomy_list_out" format="tabular" label="${tool.name} on ${on_string}: taxonomy list">
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127 <filter>write_lists and "tax" in write_lists</filter>
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128 </data>
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129 </outputs>
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130 <tests>
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131 <!-- load otu table + metadata + taxonomy -->
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132 <test expect_num_outputs="3">
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133 <param name="otutable" value="AalborgWWTPs.otu.csv"/>
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134 <param name="metadata" value="AalborgWWTPs.tsv" ftype="tsv"/>
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135 <param name="taxonomy" value="AalborgWWTPs.tax"/>
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136 <output name="ampvis" value="AalborgWWTPs.rds" ftype="ampvis2" compare="sim_size"/>
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137 <output name="metadata_list_out" value="AalborgWWTPs-metadata.list"/>
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138 <output name="taxonomy_list_out" value="AalborgWWTPs-taxonomy.list"/>
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139 <assert_stdout>
3
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140 <has_text text="ampvis2 object with 3 elements."/>
0
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141 <has_text text="575.79"/>
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142 <has_text text="SampleID, Plant, Date, Year, Period"/>
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143 <has_text text="200(100%) 194(97%) 177(88.5%) 170(85%) 152(76%) 113(56.5%) 2(1%)"/>
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144 </assert_stdout>
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145 </test>
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146 <!-- load otu table + metadata + taxonomy + tree + fasta -->
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147 <test expect_num_outputs="3">
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148 <param name="otutable" value="AalborgWWTPs.otu.csv"/>
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149 <param name="metadata" value="AalborgWWTPs.tsv" ftype="tsv"/>
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150 <param name="taxonomy" value="AalborgWWTPs.tax"/>
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151 <param name="fasta" value="AalborgWWTPs.fa" ftype="fasta"/>
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152 <param name="tree" value="AalborgWWTPs.nwk" ftype="newick"/>
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153 <output name="ampvis" value="AalborgWWTPs-complete.rds" ftype="ampvis2" compare="sim_size"/>
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154 <output name="metadata_list_out" value="AalborgWWTPs-metadata.list"/>
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155 <output name="taxonomy_list_out" value="AalborgWWTPs-taxonomy.list"/>
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156 <assert_stdout>
3
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157 <has_text text="ampvis2 object with 5 elements."/>
0
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158 <has_text text="575.79"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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159 <has_text text="SampleID, Plant, Date, Year, Period"/>
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160 <has_text text="200(100%) 194(97%) 177(88.5%) 170(85%) 152(76%) 113(56.5%) 2(1%)"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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161 </assert_stdout>
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162 </test>
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163 <!-- test biom 1/2 input (taken from https://github.com/biocore/biom-format/tree/master/examples)
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164 metadata seems not to be loaded from a biom file https://github.com/MadsAlbertsen/ampvis2/issues/129
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165 taxonomy is loaded from all but 1
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166 -->
3
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167 <test expect_num_outputs="1">
0
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168 <param name="otutable" value="rich-dense.biom" ftype="biom1"/>
3
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parents: 2
diff changeset
169 <param name="write_lists" value=""/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
170 <output name="ampvis" ftype="ampvis2">
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
171 <assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
172 <has_size value="748"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
173 </assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
174 </output>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
175 <assert_stdout>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
176 <has_text text="ampvis2 object with 3 elements."/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
177 <has_text text="4.5"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
178 <has_text text="SampleID, BarcodeSequence, LinkerPrimerSequence, BODY_SITE, Description"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
179 <has_text text="5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 1(20%)"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
180 </assert_stdout>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
181 </test>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
182 <test expect_num_outputs="1">
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
183 <param name="otutable" value="rich-sparse.biom" ftype="biom1"/>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
184 <param name="write_lists" value=""/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
185 <output name="ampvis" ftype="ampvis2">
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
186 <assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
187 <has_size value="751"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
188 </assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
189 </output>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
190 <assert_stdout>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
191 <has_text text="ampvis2 object with 3 elements."/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
192 <has_text text="4.5"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
193 <has_text text="SampleID, BarcodeSequence, LinkerPrimerSequence, BODY_SITE, Description"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
194 <has_text text="5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 1(20%)"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
195 </assert_stdout>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
196 </test>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
197 <!-- input file seems to miss metadata check that no metadata & taxonomy is loaded (ampvis2 adds dummy metadata) -->
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
198 <test expect_num_outputs="1">
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
199 <param name="otutable" value="min_sparse_otu_table_hdf5.biom" ftype="biom2"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
200 <output name="ampvis" ftype="ampvis2">
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
201 <assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
202 <has_size value="395"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
203 </assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
204 </output>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
205 <param name="write_lists" value=""/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
206 <assert_stdout>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
207 <has_text text="ampvis2 object with 3 elements."/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
208 <has_text text="4.5"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
209 <has_text text="SampleID, DummyVariable"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
210 <has_text text="0(0%) 0(0%) 0(0%) 0(0%) 0(0%) 0(0%) 0(0%)"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
211 </assert_stdout>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
212 </test>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
213 <test expect_num_outputs="1">
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
214 <param name="otutable" value="rich_sparse_otu_table_hdf5.biom" ftype="biom2"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
215 <output name="ampvis" ftype="ampvis2">
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
216 <assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
217 <has_size value="753"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
218 </assert_contents>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
219 </output>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
220 <param name="write_lists" value=""/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
221 <assert_stdout>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
222 <has_text text="ampvis2 object with 3 elements."/>
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
223 <has_text text="4.5"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
224 <has_text text="SampleID, BODY_SITE, BarcodeSequence, Description, LinkerPrimerSequence"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
225 <has_text text="5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 5(100%) 1(20%)"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
226 </assert_stdout>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
227 </test>
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
228 <!-- load dada2 ASV table + metadata + taxonomy -->
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
229 <test expect_num_outputs="3">
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
230 <param name="otutable" value="dada2-removeBimeraDenovo.tab" ftype="dada2_sequencetable"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
231 <param name="metadata" value="dada2-metadata.tsv" ftype="tsv"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
232 <param name="taxonomy" value="dada2-assignTaxonomy.tabular"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
233 <param name="asv_otu_col_empty" value="true"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
234 <param name="asv_sequences" value="true"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
235 <output name="ampvis" ftype="ampvis2">
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
236 <assert_contents>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
237 <has_size min="100"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
238 </assert_contents>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
239 </output>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
240 <output name="metadata_list_out">
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
241 <assert_contents>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
242 <has_n_lines n="23"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
243 <has_n_columns n="4"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
244 <has_text text="Sample"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
245 </assert_contents>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
246 </output>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
247 <output name="taxonomy_list_out">
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
248 <assert_contents>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
249 <has_n_lines n="370"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
250 <has_n_columns n="2"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
251 <has_line line="Bacteria&#009;Kingdom"/>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
252 </assert_contents>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
253 </output>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
254 <assert_stdout>
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
255 <has_text text="ampvis2 object with 4 elements."/> <!-- this also has fasta, i.e. 4 -->
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256 <has_text text="6212.45"/>
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257 <has_text text="Sample, time"/>
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258 <has_text text="232(100%) 232(100%) 232(100%) 231(99.57%) 209(90.09%) 127(54.74%)"/>
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259 </assert_stdout>
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260 </test>
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261 <!-- load data from phyloseq -->
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262 <test expect_num_outputs="3">
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263 <param name="otutable" value="output.phyloseq" ftype="phyloseq"/>
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264 <output name="ampvis" ftype="ampvis2">
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265 <assert_contents>
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266 <has_size min="100"/>
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267 </assert_contents>
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268 </output>
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269 <output name="metadata_list_out">
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270 <assert_contents>
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271 <has_n_lines n="6"/>
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272 <has_n_columns n="4"/>
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273 <has_text text="SampleID"/>
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274 </assert_contents>
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275 </output>
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276 <output name="taxonomy_list_out">
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277 <assert_contents>
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278 <has_n_lines n="147"/>
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279 <has_n_columns n="2"/>
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280 <has_line line="Bacteria&#009;Kingdom"/>
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281 </assert_contents>
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282 </output>
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283 <assert_stdout>
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284 <has_text text="ampvis2 object with 4 elements."/> <!-- this also has fasta, i.e. 4 -->
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285 <has_text text="SampleID, Property, Number"/>
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286 <has_text text="64(100%) 64(100%) 64(100%) 64(100%) 62(96.88%) 56(87.5%) 0(0%)"/>
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287 </assert_stdout>
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288 </test>
0
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289 </tests>
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290 <help><![CDATA[
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291
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292 What it does
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293 ============
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294
3
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295 This tool reads an OTU or ASV table and corresponding sample metadata, and returns
0
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296 a RDS data set for use in all ampvis2 tools. It is therefore required to load
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297 data with this tool before any other ampvis2 tools can be used.
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298
2
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299 The Galaxy tool calls the `amp_load <https://kasperskytte.github.io/ampvis2/reference/amp_load.html>`_
0
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300 function of the ampvis2 package. This function validates and corrects the
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301 provided data frames in different ways to make it suitable for the rest of the
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302 ampvis2 tools. It is important that the provided data sets match the
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303 requirements as described in the following to work properly.
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304
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305 Input
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306 =====
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307
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308 **The OTU-table**
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309
3
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310 contains information about the OTU/ASVs, their read counts in each sample, and
0
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311 optionally their assigned taxonomy. The OTU table can be given as
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312
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313 - Tabular data set
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314 - BIOM version (1 and 2)
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315
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316 Metadata and taxonomy in the tabular or BIOM files that are given via the
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317 ``OTU table`` parameter can is overwritten if by data presented via the
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318 ``Sample metadata`` or ``Taxonomy table`` parameters.
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319
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320 If given in tabular format the provided OTU-table must be a table with the
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321 following requirements:
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322
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323 - The rows are OTU IDs and the columns are samples.
3
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324 - The OTU IDs are by default expected to be in a column called "OTU", "ASV", or "#OTU ID".
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325 For data using an empty header for the OTU/ASV colum enable the option *OTU/ASV column has empty header*
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326 (this allows to process data as produced e.g. by dada2).
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327 - The column names of the table are the sample IDs, exactly matching those in
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328 the metadata
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329 - The last 7 columns are optionally the corresponding taxonomy assigned to the
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330 OTUs, named "Kingdom", "Phylum", "Class", "Order", "Family", "Genus", "Species".
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331
3
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332 If the ASV IDs are actually the ASV Sequences then enabling
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333 *ASV identifiers are the ASV sequences* will rename the identifiers to ASV1, ASV2,...
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334 (and save the sequences in the ampvis2 object).
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335
0
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336 Generally avoid special characters and spaces in row- and column names.
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337
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338 The OTU table can also contain the taxonomic information in additional columns:
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339 Kingdom, Phylum, Class, Order, Family, Genus.
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340
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341 Check `here <https://biom-format.org/>`_ for information on the BIOM formats.
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342
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343 **The metadata**
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
344
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
345 contains additional information about the samples, for example where each sample
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
346 was taken, date, pH, treatment etc, which is used to compare and group the
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
347 samples during analysis. The amount of information in the metadata is unlimited,
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
348 it can contain any number of columns (variables), however there are a few
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
349 requirements:
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
350
3
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
351 - The sample IDs must be in the first column. The sample IDs must match exactly
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
352 to those in the OTU-table. Any unmatched samples between the otutable and
932d7573a561 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 5b6fb9641a1320e13aba367c4e7bc52ae064acc6
iuc
parents: 2
diff changeset
353 metadata will be removed with a warning.
0
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
354 - Generally avoid special characters and spaces in row- and column names.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
355
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
356 By default the data types of metadata columns are guessed with
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
357 ``readr::type_convert``. The guessed column types can be seen in the last (4th)
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
358 column of the ``metadata list`` output and also stdout of the tool. Guessing of
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
359 data types can be disabled using the parameter ``Guess metadata column types``.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
360 If disabled matadata from separate tabular input is treated as character data,
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
361 and if loaded from biom files that data is used as is. Metadata types can be set
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
362 manually using the tool ``ampvis2: set metadata``
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
363
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
364 Dates should be given in the format ``YYYY-MM-DD`` (Y: year, M: month, D: day).
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
365
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
366 In addition to the RDS data set a metadata (resp. taxonomy) list data set is returned
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
367 if metadata (resp. taxonomic information) is given to this tool. It contains
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
368 restructured metadata (taxonomic information) that is used in downstream ampvis2
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
369 Galaxy tools in order to select metadata / metadata values (resp. taxonomic levels).
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
370
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
371 **Taxonomy**
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
372
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
373 is a tabular data set with 7 columns and one row per ASV/OTU:
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
374
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
375 - the 1st column is identical to the 1st column of the OTU table parameter
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
376 - the remaining columns contain data for Kingdom, Phylum, Class, Order, Family, Genus
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
377
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
378 Note that the taxonomic information can also be embedded in the OTU table.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
379
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
380 **Tree**
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
381
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
382 a tree with branch lengths in Newick format.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
383
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
384 This is needed / usefull only if the data is used as input of: ``ampvis2:
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
385 ordination plot`` for ordination methods NNDS / MMDS with (un)weighted UniFrac
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
386 distances. Note that the loaded tree is also filtered by the ``ampvis2: subset
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
387 ...`` tools.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
388
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
389 **Fasta**
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
390
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
391 a fasta file containing the sequences of the OTUs. Note that this information is
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
392 only used in ``ampvis2: export fasta``. If the OTU table is modified by
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
393 ``ampvis2: mergereplicates`` or the ``ampvis2: subset ...`` tools this might be
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
394 useful to obtain a filtered list of sequences.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
395
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
396
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
397 Output
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
398 ======
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
399
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
400 **RDS**
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
401
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
402 The main output of the tool is an RDS data set that contains the R representation of
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
403 the ampvis2 object containing the provided data (OTU table, metadata, taxonomy,
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
404 phylogenetic tree, and fasta).
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
405
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
406 **List files**
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
407
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
408 Summarize the metadata and taxonomy information:
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
409
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
410 - the taxonomy list file lists all taxa in a 1 column tabular data set
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
411 - the metadata list file lists the Metadata variables (column 1), and the corresponding
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
412 available metadata values (column 2), if the variable is the SampleID (column 3), and
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
413 the data type of the corresponding metadata variable (column 4)
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
414
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
415 These files are auxilliary files that are needed in downstream ``ampvis2`` Galaxy tools
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
416 to allow selecting metadata and taxonomy. They are not passed to the underlying R functions.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
417
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
418 Note that, if the no taxonomy (or metadata) is given then the underlying ``ampvis2`` R
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
419 function adds dummy taxonomy (resp. metadata). In this case the output of the list datasets
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
420 can be disabled with the ``Output list data sets`` parameter.
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
421 ]]></help>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
422 <expand macro="citations"/>
474bbc45ddd9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff changeset
423 </tool>