Mercurial > repos > iuc > ampvis2_otu_network
comparison macros.xml @ 0:4bcb464fb3f3 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
| author | iuc |
|---|---|
| date | Mon, 04 Apr 2022 10:24:03 +0000 |
| parents | |
| children | 354d645455cd |
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| -1:000000000000 | 0:4bcb464fb3f3 |
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| 1 <macros> | |
| 2 <token name="@TOOL_VERSION@">2.7.17</token> | |
| 3 <token name="@VERSION_SUFFIX@">0</token> | |
| 4 <token name="@PROFILE@">22.01</token> | |
| 5 <xml name="header"> | |
| 6 <xrefs> | |
| 7 <xref type="bio.tools">ampvis</xref> | |
| 8 </xrefs> | |
| 9 <requirements> | |
| 10 <requirement type="package" version="@TOOL_VERSION@">r-ampvis2</requirement> | |
| 11 <requirement type="package" version="2.1.1">r-readr</requirement> | |
| 12 </requirements> | |
| 13 <version_command><![CDATA[ | |
| 14 echo $(R --version | grep "R version")", ampvis2 version" $(R --vanilla --slave -e "library(ampvis2, quietly = TRUE); sessionInfo()\$otherPkgs\$ampvis2\$Version" 2> /dev/null | grep -v -i "WARNING: ") | |
| 15 ]]></version_command> | |
| 16 | |
| 17 </xml> | |
| 18 <xml name="citations"> | |
| 19 <citations> | |
| 20 <citation type="doi">10.1101/299537</citation> | |
| 21 <yield/> | |
| 22 </citations> | |
| 23 </xml> | |
| 24 | |
| 25 <xml name="rds_input_macro"> | |
| 26 <param argument="data" type="data" format="ampvis2" label="Ampvis2 RDS dataset" help="Generated with ampvis2: load"/> | |
| 27 </xml> | |
| 28 | |
| 29 <xml name="rds_metadata_input_macro" token_metadata_optional="true"> | |
| 30 <expand macro="rds_input_macro"/> | |
| 31 <param name="metadata_list" type="data" optional="@METADATA_OPTIONAL@" format="tabular" label="Metadata list"> | |
| 32 <validator type="expression" message="The metadatalist file must have 3 columns"><![CDATA[value.metadata.columns == 4]]></validator> | |
| 33 </param> | |
| 34 </xml> | |
| 35 | |
| 36 <token name="@SAVE_TAX_LIST@"><![CDATA[ | |
| 37 for(level in colnames(data\$tax)){ | |
| 38 for(u in unique(data\$tax[level])){ | |
| 39 write(u, file="$taxonomy_list_out", append=T) | |
| 40 } | |
| 41 } | |
| 42 ]]></token> | |
| 43 | |
| 44 <token name="@SAVE_METADATA_LIST@"><![CDATA[ | |
| 45 classes <- sapply(data\$metadata, class) | |
| 46 for(name in names(data\$metadata)){ | |
| 47 ## check if it is the column containing the sample names | |
| 48 ## the additional column in the ist file can the be used | |
| 49 ## to filter SampleIDs from select inputs | |
| 50 ## (check for character columns only .. since data in the column is otherwise converted to the corresponding type .. which fails) | |
| 51 if(classes[[name]] == "character" && all(data\$metadata[[name]] == rownames(data\$metadata))){ | |
| 52 sample_names <- TRUE; | |
| 53 }else{ | |
| 54 sample_names <- FALSE; | |
| 55 } | |
| 56 for(m in unique(data\$metadata[[name]])){ | |
| 57 write(paste(name, m, sample_names, classes[[name]], sep="\t"), file="$metadata_list_out", append=T); | |
| 58 } | |
| 59 } | |
| 60 ]]></token> | |
| 61 | |
| 62 <!-- defaults can be set by redefining an on option with selected="true" --> | |
| 63 <xml name="taxlevel_macro" token_argument="" token_multiple="false" token_optional="false" token_label="" token_help=""> | |
| 64 <param argument="@ARGUMENT@" type="select" optional="@OPTIONAL@" multiple="@MULTIPLE@" label="@LABEL@" help="@HELP@"> | |
| 65 <option value="OTU">OTU/ASV</option> | |
| 66 <option value="Species">Species</option> | |
| 67 <option value="Genus">Genus</option> | |
| 68 <option value="Family">Family</option> | |
| 69 <option value="Order">Order</option> | |
| 70 <option value="Class">Class</option> | |
| 71 <option value="Phylum">Phylum</option> | |
| 72 <option value="Kingdom">Kingdom</option> | |
| 73 <yield/> | |
| 74 </param> | |
| 75 </xml> | |
| 76 | |
| 77 <xml name="tax_empty_macro"> | |
| 78 <param argument="tax_empty" type="select" label="How to show OTUs without taxonomic information"> | |
| 79 <option value="remove">Remove OTUs without taxonomic information</option> | |
| 80 <option value="best" selected="true">Use the best classification possible</option> | |
| 81 <option value="OTU">Display the OTU name</option> | |
| 82 </param> | |
| 83 </xml> | |
| 84 | |
| 85 <token name="@TAX_SHOW@"><![CDATA[ | |
| 86 #if $tax_show_cond.tax_show_sel == "number" | |
| 87 tax_show = $tax_show_cond.tax_show, | |
| 88 #else | |
| 89 #set tax_show_list='c("' + '", "'.join(str($tax_show_cond.tax_show).split(",")) + '")' | |
| 90 tax_show = $tax_show_list | |
| 91 #end if | |
| 92 ]]></token> | |
| 93 <xml name="tax_show_macro" token_value=""> | |
| 94 <conditional name="tax_show_cond"> | |
| 95 <param name="tax_show_sel" type="select" label="Limit the number of shown taxa"> | |
| 96 <option value="number">Select a number of taxa to show</option> | |
| 97 <option value="explicit">Manually select taxa to show</option> | |
| 98 </param> | |
| 99 <when value="number"> | |
| 100 <param argument="tax_show" type="integer" value="@VALUE@" min="1" label="Number of taxa to show"/> | |
| 101 </when> | |
| 102 <when value="explicit"> | |
| 103 <param name="taxonomy_list" format="tabular" type="data" optional="false" label="Taxonomy list" help="Generated with ampvis2: load"/> | |
| 104 <param argument="tax_show" type="select" multiple="true" optional="false" label="Taxa to show"> | |
| 105 <options from_dataset="taxonomy_list"> | |
| 106 <column name="name" index="0"/> | |
| 107 <column name="value" index="0"/> | |
| 108 <!-- TODO filter by tax_aggregate level? --> | |
| 109 </options> | |
| 110 </param> | |
| 111 </when> | |
| 112 </conditional> | |
| 113 </xml> | |
| 114 | |
| 115 <xml name="metadata_select" token_quote="__" tokens="argument,label" token_an="argument" token_optional="true" token_multiple="false" token_help=""> | |
| 116 <param __AN__="__ARGUMENT__" type="select" optional="__OPTIONAL__" multiple="__MULTIPLE__" label="__LABEL__" help="In order to select values a metadata list needs to be selected first. __HELP__"> | |
| 117 <options from_dataset="metadata_list"> | |
| 118 <column name="name" index="0"/> | |
| 119 <column name="value" index="0"/> | |
| 120 <filter type="unique_value" column="0"/> | |
| 121 <yield name="options"/> | |
| 122 </options> | |
| 123 <yield/> | |
| 124 </param> | |
| 125 </xml> | |
| 126 | |
| 127 <xml name="metadata_select_discrete" tokens="argument,label" token_optional="true" token_multiple="false" token_help=""> | |
| 128 <expand macro="metadata_select" argument="@ARGUMENT@" optional="@OPTIONAL@" multiple="@MULTIPLE@" label="@LABEL@" help="@HELP@"> | |
| 129 <token name="options"> | |
| 130 <filter type="static_value" column="3" value="numeric" keep="false"/> | |
| 131 </token> | |
| 132 <yield/> | |
| 133 </expand> | |
| 134 </xml> | |
| 135 | |
| 136 <xml name="metadata_select_numeric" tokens="argument,label" token_optional="true" token_multiple="false" token_help=""> | |
| 137 <expand macro="metadata_select" argument="@ARGUMENT@" optional="@OPTIONAL@" multiple="@MULTIPLE@" label="@LABEL@" help="@HELP@"> | |
| 138 <token name="options"> | |
| 139 <filter type="static_value" column="3" value="character" keep="false"/> | |
| 140 <filter type="static_value" column="3" value="Date" keep="false"/> | |
| 141 </token> | |
| 142 <yield/> | |
| 143 </expand> | |
| 144 </xml> | |
| 145 | |
| 146 <xml name="metadata_select_date" tokens="argument,label" token_optional="true" token_multiple="false" token_help=""> | |
| 147 <expand macro="metadata_select" argument="@ARGUMENT@" optional="@OPTIONAL@" multiple="@MULTIPLE@" label="@LABEL@" help="@HELP@"> | |
| 148 <token name="options"> | |
| 149 <filter type="static_value" column="3" value="Date" keep="true"/> | |
| 150 </token> | |
| 151 <yield/> | |
| 152 </expand> | |
| 153 </xml> | |
| 154 | |
| 155 <xml name="metadata_sample_select" token_argument=""> | |
| 156 <conditional name="@ARGUMENT@_cond"> | |
| 157 <param name="@ARGUMENT@_sel" type="select"> | |
| 158 <option value="no">No</option> | |
| 159 <option value="variable">Metadata variable</option> | |
| 160 <option value="sample">Specific sample</option> | |
| 161 </param> | |
| 162 <when value="no"/> | |
| 163 <when value="variable"> | |
| 164 <expand macro="metadata_select" argument="@ARGUMENT@" label="Metadata variable"/> | |
| 165 </when> | |
| 166 <when value="sample"> | |
| 167 <param argument="@ARGUMENT@" type="select" optional="true"> | |
| 168 <options from_dataset="metadata_list"> | |
| 169 <column name="name" index="1"/> | |
| 170 <column name="value" index="1"/> | |
| 171 <filter type="static_value" value="TRUE" column="2"/> <!-- filter samples --> | |
| 172 <filter type="unique_value" column="1"/> | |
| 173 </options> | |
| 174 </param> | |
| 175 </when> | |
| 176 </conditional> | |
| 177 </xml> | |
| 178 | |
| 179 <xml name="taxonomy_select_macro" token_argument="" token_optional="false" token_multiple="true"> | |
| 180 <param argument="@ARGUMENT@" type="select" optional="@OPTIONAL@" multiple="@MULTIPLE@"> | |
| 181 <options from_dataset="taxonomy_list"> | |
| 182 <column name="name" index="0"/> | |
| 183 <column name="value" index="0"/> | |
| 184 <filter type="unique_value" column="0"/> | |
| 185 </options> | |
| 186 <yield/> | |
| 187 </param> | |
| 188 </xml> | |
| 189 | |
| 190 <xml name="facet_scales_macro" token_argument="" token_label="Scales of the facets" token_help=""> | |
| 191 <param argument="@ARGUMENT@" type="select" optional="true" label="@LABEL@" help="@HELP@"> | |
| 192 <option value="fixed">Same scale</option> | |
| 193 <option value="free">Free scale</option> | |
| 194 <option value="free_x">Free x-scale</option> | |
| 195 <option value="free_y">Free y-scale</option> | |
| 196 </param> | |
| 197 </xml> | |
| 198 | |
| 199 <token name="@OUTPUT_TOKEN@"> | |
| 200 ggsave("$plot", | |
| 201 print(plot), | |
| 202 device="$output_options.out_format" | |
| 203 #if str($output_options.plot_width) != '' | |
| 204 , width = $output_options.plot_width | |
| 205 #end if | |
| 206 #if str($output_options.plot_height) != '' | |
| 207 , height = $output_options.plot_height | |
| 208 #end if | |
| 209 ) | |
| 210 </token> | |
| 211 <xml name="out_macro" token_name="plot" token_label=""> | |
| 212 <data name="@NAME@" format="pdf" label="${tool.name} on ${on_string}@LABEL@"> | |
| 213 <yield/> | |
| 214 <change_format> | |
| 215 <when input="output_options.out_format" value="png" format="png" /> | |
| 216 <when input="output_options.out_format" value="svg" format="svg" /> | |
| 217 </change_format> | |
| 218 </data> | |
| 219 </xml> | |
| 220 <xml name="out_format_macro"> | |
| 221 <section name="output_options" title="Output options" expanded="false"> | |
| 222 <param name="out_format" type="select" label="Output format"> | |
| 223 <option value="pdf" selected="true">PDF</option> | |
| 224 <option value="png">PNG</option> | |
| 225 <option value="svg">SVG</option> | |
| 226 <yield/> | |
| 227 </param> | |
| 228 <param name="plot_width" type="float" value="" min="1" optional="true" label="Plot width in cm"/> | |
| 229 <param name="plot_height" type="float" value="" min="1" optional="true" label="Plot height in cm"/> | |
| 230 </section> | |
| 231 </xml> | |
| 232 | |
| 233 <token name="@RAREFY_TOKEN@"> | |
| 234 #if str($rarefy) != "" | |
| 235 , rarefy = $rarefy | |
| 236 #end if | |
| 237 </token> | |
| 238 <xml name="rarefy_macro" token_help=""> | |
| 239 <param argument="rarefy" type="integer" optional="true" value="" min="0" label="Rarefy species richness to this value" help="@HELP@"/> | |
| 240 </xml> | |
| 241 | |
| 242 <xml name="normalise_macro" token_checked="false"> | |
| 243 <param argument="normalise" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="@CHECKED@" label="Normalise read counts" help="Transform the OTU read counts to be in percent per sample."/> | |
| 244 </xml> | |
| 245 | |
| 246 <token name="@HELP_RDS_INPUT@"> | |
| 247 An ampvis2 RDS dataset obtained by the ``ampvis2: load`` tool (or ``ampvis2: mergereplicates`` or one of the | |
| 248 ``ampvis2: subset ...`` tools). | |
| 249 </token> | |
| 250 <token name="@HELP_METADATA_LIST_INPUT@"> | |
| 251 The metadata list output of the ``ampvis2: load`` tool (or ``ampvis2: mergereplicates`` or the | |
| 252 ``ampvis2: subset sample`` tool). This input is only used for providing | |
| 253 values to the parameters alowing to select metadata variables or values. | |
| 254 </token> | |
| 255 | |
| 256 <token name="@HELP_RELATIVE_ABUNDANCES@"> | |
| 257 Preserving relative abundances in a subset of larger data | |
| 258 ========================================================= | |
| 259 | |
| 260 By default the raw read counts in the abundance matrix are normalised | |
| 261 (transformed to percentages) by some ampvis2 plotting tools (for | |
| 262 example ``ampvis2: heatmap``, ``ampvis2: timeseries``, and more). | |
| 263 If this is applied to data generated by one of the ``ampvis2: subset ...`` tools | |
| 264 this means that the relative | |
| 265 abundances shown will be calculated based on the remaining taxa after the | |
| 266 subset, not including the removed taxa, if any. To circumvent this, enable | |
| 267 ``normalise`` when subsetting with the ``ampvis2: subset ...``` tools, | |
| 268 and then disable ``normalise`` in the plotting tool. This will | |
| 269 transform the OTU counts to relative abundances BEFORE the subset. | |
| 270 </token> | |
| 271 </macros> |
