Mercurial > repos > iuc > arriba
annotate macros.xml @ 0:a24ca22b906e draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
author | iuc |
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date | Wed, 27 Jul 2022 11:24:44 +0000 |
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children | 4f1efcc055d5 |
rev | line source |
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0
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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1 <macros> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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2 <token name="@TOOL_VERSION@">2.3.0</token> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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3 <token name="@VERSION_SUFFIX@">0</token> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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4 <xml name="requirements"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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5 <requirements> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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6 <requirement type="package" version="@TOOL_VERSION@">arriba</requirement> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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7 <yield/> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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8 </requirements> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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9 </xml> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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10 <xml name="citations"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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11 <citations> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
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12 <citation type="doi">10.1101/gr.257246.119</citation> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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13 <yield /> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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14 </citations> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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15 </xml> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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16 <xml name="version_command"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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17 <version_command>arriba -h | grep Version | sed 's/^.* //'</version_command> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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18 </xml> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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19 <xml name="genome_source" token_assembly_optional="false" > |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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20 <conditional name="genome"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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21 <param name="genome_source" type="select" label="Genome assembly fasta (that was used for STAR alignment)"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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22 <option value="history">From your history</option> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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23 <option value="cached">Use built-in Genome reference</option> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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24 </param> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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25 <when value="history"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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26 <param name="assembly" argument="-a" type="data" format="fasta" optional="@ASSEMBLY_OPTIONAL@" label="Genome assembly fasta"/> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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27 </when> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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28 <when value="cached"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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29 <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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30 <options from_data_table="all_fasta"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
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31 <validator type="no_options" message="No reference genomes are available" /> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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32 </options> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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33 </param> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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34 </when> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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35 </conditional> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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36 </xml> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
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37 <xml name="gtf_source" token_assembly_optional="false" > |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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38 <conditional name="genome_gtf"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
changeset
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39 <param name="gtf_source" type="select" label="Genome GTF annotation source"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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40 <option value="history">From your history</option> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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41 <!-- <option value="cached">Use built-in Gtf annotation</option> --> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
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42 </param> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
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43 <when value="history"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
diff
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44 <param name="annotation" argument="-g" type="data" format="gtf" label="Gene annotation in GTF format"/> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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45 </when> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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46 </conditional> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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47 </xml> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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48 |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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49 <token name="@GENOME_SOURCE@"><![CDATA[ |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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50 #if str($genome.genome_source) == "history" |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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51 #if $genome.assembly |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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52 #set $genome_assembly = 'genome.fa' |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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53 ln -sf '$genome.assembly' $genome_assembly && |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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54 #end if |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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55 #elif str($genome.genome_source) == "cached" |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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56 #set $genome_assembly = $genome.ref_file.fields.fasta |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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57 #end if |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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58 ]]></token> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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59 <token name="@GTF_SOURCE@"><![CDATA[ |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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60 #if str($genome_gtf.gtf_source) == "history" |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
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61 #if $genome_gtf.annotation.is_of_type('gtf.gz') |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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62 #set $genome_annotation = 'genome.gtf.gz' |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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63 #else |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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64 #set $genome_annotation = 'genome.gtf' |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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65 #end if |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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66 ln -sf '$genome_gtf.annotation' $genome_annotation && |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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67 #end if |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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68 ]]></token> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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69 |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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70 <xml name="visualization_options"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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71 <param name="cytobands" argument="--cytobands" type="data" format="tabular" optional="true" label="Cytobands"/> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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72 <section name="options" expanded="false" title="Draw Fusion Options"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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73 <param argument="--sampleName" type="text" value="" optional="true" label="Sample Name printed as the title on every page"/> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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74 <param argument="--transcriptSelection" type="select" optional="true" label="Transcript selection"> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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75 <help>By default the transcript isoform with the highest coverage is drawn. |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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76 Alternatively, the transcript isoform that is provided in the columns |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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77 transcript_id1 and transcript_id2 in the given fusions file can be drawn. |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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78 Selecting the isoform with the highest coverage usually produces nicer plots, |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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79 in the sense that the coverage track is smooth and shows a visible increase in coverage after the fusion breakpoint. |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
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80 However, the isoform with the highest coverage may not be the one that is involved in the fusion. |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
iuc
parents:
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81 Often, genomic rearrangements lead to non-canonical isoforms being transcribed. |
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82 For this reason, it can make sense to rely on the transcript selection provided by the columns transcript_id1/2, |
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83 which reflect the actual isoforms involved in a fusion. |
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84 \ As a third option, the transcripts that are annotated as canonical can be drawn. |
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85 Transcript isoforms tagged with appris_principal, appris_candidate, or CCDS are considered canonical. |
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86 </help> |
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87 <option value="coverage">coverage</option> |
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88 <option value="provided">provided</option> |
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89 <option value="canonical">canonical</option> |
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90 </param> |
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91 <param argument="--minConfidenceForCircosPlot" type="select" optional="true" label="Transcript selection"> |
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92 <help>The fusion of interest is drawn as a solid line in the circos plot. |
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93 To give an impression of the overall degree of rearrangement, |
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94 all other fusions are drawn as semi-transparent lines in the background. |
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95 This option determines which other fusions should be included in the circos plot. |
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96 Values specify the minimum confidence a fusion must have to be included. |
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97 It usually makes no sense to include low-confidence fusions in circos plots, |
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98 because they are abundant and unreliable, and would clutter up the circos plot. |
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99 Default: medium |
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100 </help> |
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101 <option value="none">none - only the fusion of interest is drawn</option> |
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102 <option value="low">low</option> |
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103 <option value="medium">medium</option> |
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104 <option value="high">high</option> |
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105 </param> |
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106 <param argument="--squishIntrons" type="select" optional="true" label="Squish introns"> |
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107 <help>Exons usually make up only a small fraction of a gene. |
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108 They may be hard to see in the plot. i |
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109 Since introns are in most situations of no interest in the context of gene fusions, |
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110 this switch can be used to shrink the size of introns to a fixed, negligible size. |
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111 It makes sense to disable this feature, if breakpoints in introns are of importance. |
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112 Default: TRUE |
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113 </help> |
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114 <option value="TRUE">True</option> |
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115 <option value="FALSE">False</option> |
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116 </param> |
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117 <param argument="--showIntergenicVicinity" type="text" value="" optional="true" label="Intergenic Vicinity"> |
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118 <help>This option only applies to intergenic breakpoints. |
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119 If it is set to a value greater than 0, then the script draws the genes |
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120 which are no more than the given distance away from an intergenic breakpoint. |
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121 The keywords closestGene and closestProteinCodingGene instruct the script |
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122 to dynamically determine the distance to the next (protein-coding) gene for each breakpoint. |
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123 Alternatively, instead of specifying a single distance |
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124 that is applied upstream and downstream of both breakpoints alike, |
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125 more fine-grained control over the region to be shown is possible by specifying four comma-separated values. |
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126 The first two values determine the region to the left and to the right of breakpoint 1; |
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127 the third and fourth values determine the region to the left and to the right of breakpoint 2. |
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128 Note that this option is incompatible with squishIntrons. |
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129 Default: 0 |
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130 </help> |
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131 <option value="closestGene">closestGene</option> |
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132 <option value="closestProteinCodingGene">closestProteinCodingGene</option> |
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133 <validator type="regex" message="">^(closestGene|closestProteinCodingGene|\d+|\d+,\d+,\d+,\d+)$</validator> |
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134 </param> |
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135 <param argument="--mergeDomainsOverlappingBy" type="float" value="" min="0." max="1.0" optional="true" label="Merge Domains Overlapping By"> |
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136 <help>Occasionally, domains are annotated redundantly. |
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137 For example, tyrosine kinase domains are frequently annotated as |
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138 Protein tyrosine kinase and Protein kinase domain. |
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139 In order to simplify the visualization, such domains can be merged into one, |
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140 given that they overlap by the given fraction. |
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141 The description of the larger domain is used. |
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142 Default: 0.9 |
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143 </help> |
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144 </param> |
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145 <param argument="--printExonLabels" type="select" optional="true" label="Print Exon Labels"> |
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146 <help>By default the number of an exon is printed inside each exon, |
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147 which is taken from the attribute exon_number of the GTF annotation. |
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148 When a gene has many exons, the boxes may be too narrow to contain the labels, |
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149 resulting in unreadable exon labels. In these situations, i |
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150 it may be better to turn off exon labels. |
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151 Default: TRUE |
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152 </help> |
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153 <option value="TRUE">True</option> |
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154 <option value="FALSE">False</option> |
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155 </param> |
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156 <param argument="--render3dEffect" type="select" optional="true" label="Render 3D effect"> |
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157 <help>Whether light and shadow should be rendered to give objects a 3D effect. |
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158 Default: TRUE |
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159 </help> |
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160 <option value="TRUE">True</option> |
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161 <option value="FALSE">False</option> |
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162 </param> |
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163 <param argument="--optimizeDomainColors" type="select" optional="true" label="Optimize Domain Colors"> |
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164 <help>By default, the script colorizes domains according to the colors |
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165 specified in the file given in --annotation. |
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166 This way, coloring of domains is consistent across all proteins. |
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167 But since there are more distinct domains than colors, |
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168 this can lead to different domains having the same color. |
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169 If this option is set to TRUE, the colors are recomputed for each fusion separately. |
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170 This ensures that the colors have the maximum distance for each individual fusion, |
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171 but they are no longer consistent across different fusions. |
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172 Default: FALSE |
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173 </help> |
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174 <option value="TRUE">True</option> |
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175 <option value="FALSE">False</option> |
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176 </param> |
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177 <param argument="--color1" type="color" value="" optional="true" label="Color of the 5' end of the fusion."/> |
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178 <param argument="--color2" type="color" value="" optional="true" label="Color of the 3' end of the fusion."/> |
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179 <param argument="--pdfWidth" type="float" value="" min="1." optional="true" label="Width of PDF output file in inches" |
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180 help="Default: 11.692"/> |
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181 <param argument="--pdfHeight" type="float" value="" min="1." optional="true" label="Height of PDF output file in inches" |
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182 help="Default: 8.267"/> |
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183 <param argument="--fontSize" type="float" value="" min="0." optional="true" label="Scale the size of text" |
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184 help="Default: 1.0"/> |
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185 <param argument="--fontFamily" type="text" value="" optional="true" label="Font to use for all labels in the plots."> |
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186 <help>Default: Helvetica |
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187 </help> |
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188 <option value="serif">serif</option> |
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189 <option value="sans">sans</option> |
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190 <option value="mono">mono</option> |
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191 <option value="AvantGarde">AvantGarde</option> |
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192 <option value="Bookman">Bookman</option> |
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193 <option value="Courier">Courier</option> |
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194 <option value="Helvetica">Helvetica</option> |
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195 <option value="Helvetica-Narrow">Helvetica-Narrow</option> |
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196 <option value="NewCenturySchoolbook">NewCenturySchoolbook</option> |
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197 <option value="Palatino">Palatino</option> |
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198 <option value="Times">Times</option> |
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199 <option value="URWGothic">URWGothic</option> |
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200 <option value="URWBookman">URWBookman</option> |
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201 <option value="NimbusMon">NimbusMon</option> |
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202 <option value="NimbusSan">NimbusSan</option> |
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203 <option value="URWHelvetica">URWHelvetica</option> |
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204 <option value="NimbusSanCond">NimbusSanCond</option> |
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205 <option value="CenturySch">CenturySch</option> |
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206 <option value="URWPalladio">URWPalladio</option> |
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207 <option value="NimbusRom">NimbusRom</option> |
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208 <option value="URWTimes">URWTimes</option> |
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209 <option value="ArialMT">ArialMT</option> |
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210 <option value="Japan1">Japan1</option> |
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211 <option value="Japan1HeiMin">Japan1HeiMin</option> |
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212 <option value="Japan1GothicBBB">Japan1GothicBBB</option> |
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213 <option value="Japan1Ryumin">Japan1Ryumin</option> |
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214 <option value="Korea1">Korea1</option> |
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215 <option value="Korea1deb">Korea1deb</option> |
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216 <option value="CNS1">CNS1</option> |
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217 <option value="GB1">GB1</option> |
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218 </param> |
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219 <param argument="--fixedScale" type="integer" value="" min="0" optional="true" label="Apply a fixed scale to all fusions"> |
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220 <help>By default, transcripts are scaled automatically to fill the entire page. |
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221 This parameter enforces a fixed scale to be applied to all fusions, |
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222 which is useful when a collection of fusions should be visualized and the sizes of all transcripts should be comparable. |
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223 A common use case is the visualization of a gene that is found to be fused to multiple partners. |
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224 By forcing all fusion plots to use the same scale, the fusions can be summarized as a collage |
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225 in a single plot one above the other with matching scales. |
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226 Note: The scale must be bigger than the sum of the biggest pair of transcripts to be drawn, |
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227 or else dynamic scaling is applied, because display errors would occur otherwise. |
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228 The default value is 0, which means that no fixed scale should be used |
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229 and that the scale should be adapted dynamically for each fusion. Default: 0 |
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230 </help> |
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231 </param> |
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232 <param argument="--coverageRange" type="text" value="" optional="true" label="Maximum coverage for plot"> |
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233 <help>When the parameter --alignments is used, coverage plots are drawn above the transcripts of the fused genes. |
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234 The plots can be cropped at a fixed level by passing a non-zero value to this parameter. |
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235 When only a single value is given, both coverage plots (for gene1 and gene2) are cropped at the same level. |
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236 When two comma-separated values are given, the cutoffs can be specified independently for the two plots. |
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237 A value of 0 indicates that no cropping should be applied (i.e., the cutoff is set to the peak coverage) |
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238 and that the coverage plots of both genes should be on the same scale. This is the default behavior. |
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239 A value of 0,0 also indicates that no cropping should be applied, |
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240 but the coverage plots of the two genes have different scales: |
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241 each one is scaled individually to the peak coverage of the respective gene. |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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242 Default: 0 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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243 </help> |
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244 <validator type="regex" message="">^\d+(,\d+)?$</validator> |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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245 </param> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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246 </section> |
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247 </xml> |
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248 <token name="@DRAW_FUSIONS@"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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249 draw_fusions.R |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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250 --fusions='$fusions' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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251 --alignments='Aligned.sortedByCoord.out.bam' |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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252 --annotation='$genome_gtf.annotation' |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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253 --output=fusions.pdf |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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254 #if $visualization.cytobands |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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255 --cytobands='$visualization.cytobands' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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256 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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257 #if $protein_domains |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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258 --proteinDomains='$protein_domains' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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259 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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260 ## Visualization Options |
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261 #if $visualization.options.transcriptSelection |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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262 --transcriptSelection=$visualization.options.transcriptSelection |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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263 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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264 #if $visualization.options.minConfidenceForCircosPlot |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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265 --minConfidenceForCircosPlot=$visualization.options.minConfidenceForCircosPlot |
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266 #end if |
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267 #if $visualization.options.squishIntrons |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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268 --squishIntrons=$visualization.options.squishIntrons |
a24ca22b906e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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269 #if $visualization.options.squishIntrons == 'FALSE' and $visualization.options.showIntergenicVicinity |
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270 --showIntergenicVicinity='$visualization.options.showIntergenicVicinity' |
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271 #end if |
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272 #end if |
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273 #if $visualization.options.mergeDomainsOverlappingBy |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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274 --mergeDomainsOverlappingBy=$visualization.options.mergeDomainsOverlappingBy |
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275 #end if |
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276 #if $visualization.options.sampleName |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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277 --sampleName='$visualization.options.sampleName' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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278 #end if |
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279 #if $visualization.options.printExonLabels |
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280 --printExonLabels=$visualization.options.printExonLabels |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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281 #end if |
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282 #if $visualization.options.coverageRange |
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283 --coverageRange='$visualization.options.coverageRange' |
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284 #end if |
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285 #if $visualization.options.render3dEffect |
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286 --render3dEffect=$visualization.options.render3dEffect |
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287 #end if |
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288 #if $visualization.options.optimizeDomainColors |
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289 --optimizeDomainColors=$visualization.options.optimizeDomainColors |
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290 #end if |
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291 #if $visualization.options.color1 |
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292 --color1='$visualization.options.color1' |
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293 #end if |
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294 #if $visualization.options.color2 |
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295 --color2='$visualization.options.color2' |
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296 #end if |
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297 #if str($visualization.options.pdfWidth) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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298 --pdfWidth=$visualization.options.pdfWidth |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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299 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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300 #if str($visualization.options.pdfHeight) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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301 --pdfHeight=$visualization.options.pdfHeight |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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302 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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303 # fontFamily |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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304 #if $visualization.options.fontFamily |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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305 --fontFamily=$visualization.options.fontFamily |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/arriba commit b12158e6cc9b1b2bd6e7522dfc183e9055575823
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306 #end if |
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307 #if str($visualization.options.fontSize) |
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308 --fontSize=$visualization.options.fontSize |
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309 #end if |
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310 </token> |
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311 </macros> |