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annotate art_illumina.xml @ 2:7a12314c5b64 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/art commit 97a064489364118d108b0dd2cfb4d9bc53080837-dirty
author iuc
date Thu, 19 Nov 2015 12:32:17 -0500
parents a12ce5668966
children bb7e0ccc0029
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b98d6fffd00b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/art commit 2b8fe4bffea74c80e20d2d4d0c426cc1631fc05f
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1 <tool id="art_illumina" name="ART Illumina" version="2014.11.03.0">
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2 <description>simulates sequencing data</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
1
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6 <expand macro="requirements"/>
0
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7 <expand macro="stdio" />
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8 <command><![CDATA[
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9 art_illumina
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10
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11 #if str($sam) == "-s":
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12 --samout
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13 #end if
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14
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15 #if not str($aln) == "-a":
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16 --noALN
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17 #end if
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18
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19 #if str($generate.choice) == "paired_end":
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20 --paired
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21 --mflen $generate.fragment_size
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22 --sdev $generate.fragment_sd
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23 #else if str($generate.choice) == "mate_pair":
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24 --matepair
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25 --mflen $generate.fragment_size
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26 --sdev $generate.fragment_sd
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27 #end if
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28
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29 --in $input_seq_file
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30 --len $read_length
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31 --fcov $fold_coverage
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32
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33 $amplicon
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34
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35 --insRate $insRate
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36 --insRate2 $insRate2
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37 --delRate $delRate
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38 --delRate2 $delRate2
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39
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40 #if $rndSeed and $rndSeed > -1:
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41 --rndSeed $rndSeed
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42 #end if
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43
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44 --out output;
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45 ]]></command>
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46 <inputs>
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47 <param label="DNA/RNA reference sequence" format="fasta" name="input_seq_file" type="data"/>
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48 <param label="the fold of read coverage over the reference sequences" name="fold_coverage" type="integer" value="20"/>
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49 <param label="length of reads to be simulated" name="read_length" type="integer" value="100"/>
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50 <param label="amplicon sequencing simulation" name="amplicon" type="boolean" truevalue="--amplicon" falsevalue="" />
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51
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52 <conditional name="generate">
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53 <param name="choice" type="select" label="Type of data to generate">
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54 <option value="single_end">Single-End</option>
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55 <option value="paired_end">Paired-End</option>
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56 <option value="mate_pair">Mate-Pair</option>
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57 </param>
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58 <when value="single_end">
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59 </when>
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60 <when value="paired_end">
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61 <expand macro="frag_len_sd" />
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62 </when>
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63 <when value="mate_pair">
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64 <expand macro="frag_len_sd" />
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65 </when>
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66 </conditional>
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67
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68 <expand macro="aln" />
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69 <expand macro="sam" />
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70
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71 <param label="the first-read insertion rate" help="(--insRate)" name="insRate" type="float" value="0.00009" />
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72 <param label="the second-read insertion rate" help="(--insRate2)" name="insRate2" type="float" value="0.00015" />
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73 <param label="the first-read deletion rate" help="(--delRate)" name="delRate" type="float" value="0.00011" />
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74 <param label="the second-read deletion rate" help="(--delRate2)" name="delRate2" type="float" value="0.00023" />
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75
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76 <expand macro="rndSeed" />
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77 <!-- todo: id, errfree, masN, qShift(2), sepProf -->
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78 </inputs>
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79 <outputs>
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80 <!-- Single End -->
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81 <data format="fastq" name="output_fq1_single" from_work_dir="output.fq" label="Simulated of Illumina sequencing of $input_seq_file.name">
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82 <filter>generate['choice'] == "single_end"</filter>
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83 </data>
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84
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85 <!-- Paired End -->
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86 <data format="fastq" name="output_fq1_paired" from_work_dir="output1.fq" label="Simulated of Illumina sequencing of $input_seq_file.name (Forward)">
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87 <filter>generate['choice'] != "single_end"</filter>
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88 </data>
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89 <data format="fastq" name="output_fq2_paired" from_work_dir="output2.fq" label="Simulated of Illumina sequencing of $input_seq_file.name (Reverse)">
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90 <filter>generate['choice'] != "single_end"</filter>
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91 </data>
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92 <data format="sam" name="output_sam" from_work_dir="output.sam" label="Mapping of Simulated Illumina data to $input_seq_file.name">
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93 <filter>sam</filter>
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94 </data>
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95
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96 <!-- Single End -->
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97 <data format="aln" name="output_aln1_single" from_work_dir="output.aln" label="Alignment of Simulated Illumina data to $input_seq_file.name">
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98 <filter>aln and generate['choice'] == "single_end"</filter>
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99 </data>
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100
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101 <!-- paired end -->
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102 <data format="aln" name="output_aln1_paired" from_work_dir="output1.aln" label="Alignment of Simulated Illumina data to $input_seq_file.name">
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103 <filter>aln and generate['choice'] != "single_end"</filter>
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104 </data>
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105 <data format="aln" name="output_aln2_paired" from_work_dir="output2.aln" label="Alignment of Simulated Illumina data to $input_seq_file.name">
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106 <filter>aln and generate['choice'] != "single_end"</filter>
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107 </data>
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108 </outputs>
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109 <tests>
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110 <test>
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111 <param name="rndSeed" value="42" />
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112 <param name="input_seq_file" value="input.fa" />
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113 <param name="fold_coverage" value="20" />
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114 <param name="read_length" value="200" />
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115 <param name="choice" value="single_end" />
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116 <param name="aln" value="True" />
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117 <param name="sam" value="True" />
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118
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119 <output name="output_fq1_single" file="art.illumina.01.fq" />
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120 <output name="output_aln1_single" file="art.illumina.01.aln" lines_diff="2"/>
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121 <output name="output_sam" file="art.illumina.01.sam" lines_diff="2"/>
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122 </test>
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123 <test>
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124 <param name="rndSeed" value="42" />
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125 <param name="input_seq_file" value="input.fa" />
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126 <param name="fold_coverage" value="20" />
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127 <param name="read_length" value="200" />
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128 <param name="choice" value="paired_end" />
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129 <param name="fragment_size" value="300" />
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130 <param name="fragment_sd" value="10" />
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131 <param name="aln" value="True" />
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132 <param name="sam" value="True" />
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133
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134 <output name="output_fq1_paired" file="art.illumina.021.fq" />
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135 <output name="output_fq2_paired" file="art.illumina.022.fq" />
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136 <output name="output_aln1_paired" file="art.illumina.021.aln" lines_diff="2"/>
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137 <output name="output_aln2_paired" file="art.illumina.022.aln" lines_diff="2"/>
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138 <output name="output_sam" file="art.illumina.02.sam" lines_diff="2"/>
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139 </test>
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140 </tests>
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141 <help><![CDATA[
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142 Art Illumina Simulator
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143 ======================
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144
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145 ART (art_Illumina Q version) is a simulation program to generate sequence read
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146 data of Illumina sequencers. ART generates reads according to the empirical
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147 read quality profile summarized from large real read data. ART has been using
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148 for testing or benchmarking a variety of methods or tools for next-generation
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149 sequencing data analysis, including read alignment, de novo assembly, detection
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150 of SNP, CNV, or other structure variation.
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151
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152 art_Illumina can generate single-end, paired-end, mate-pair reads, and
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153 amplicon sequencing simulation of Illumina sequencing platform. Its outputs
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154 include FASTQ read, ALN and/or SAM alignment files.
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155
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156 ]]></help>
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157 <expand macro="citation" />
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158 </tool>
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159