--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/artic_minion.xml	Thu Apr 30 04:05:09 2020 -0400
@@ -0,0 +1,110 @@
+<tool id="artic_minion" name="ARTIC minion" version="1.1.0_rc2+galaxy0">
+    <description></description>
+    <requirements>
+        <requirement type="package" version="1.1.0_rc2">artic</requirement>
+    </requirements>
+    <command detect_errors="exit_code"><![CDATA[
+        mkdir -p 'scheme/name/version' &&
+        #if str( $primer_scheme_source.primer_scheme_source_selector ) == "tool_data_table":
+          ln -s '${primer_scheme_source.primer_scheme_bedfile.fields.path}' 'scheme/name/version/name.scheme.bed' &&
+        #else:
+          ln -s '${primer_scheme_source.primer_scheme_bedfile}' 'scheme/name/version/name.scheme.bed' &&
+        #end if
+        #if str( $reference_source.reference_source_selector ) == "history":
+          ln -s '${reference_source.reference}' 'scheme/name/version/name.reference.fasta' &&
+          samtools faidx 'scheme/name/version/name.reference.fasta' &&
+        #else:
+          ln -s '${reference_source.reference.fields.path}' 'scheme/name/version/name.reference.fasta' &&
+          samtools faidx 'scheme/name/version/name.reference.fasta' &&
+        #end if
+        artic minion
+            --threads \${GALAXY_SLOTS:-1}
+            --normalise '${normalise}'
+            --read-file '${read_file}'
+            --scheme-directory 'scheme'
+            --medaka
+            $bwa
+            'name/version'
+            '${read_file.element_identifier}'
+    ]]></command>
+    <inputs>
+        <param argument="--read-file" type="data" format="fastq,fastqsanger,fastq.gz,fastqsanger.gz" label="Input Read File"/>
+        <param argument="--normalise" type="integer" min="1" value="200" max="2000" label="Coverage normalisation depth"/>
+        <param argument="--bwa" type="boolean" truevalue="--bwa" falsevalue="" label="Use bwa aligner"/>
+        <conditional name="primer_scheme_source">
+            <param name="primer_scheme_source_selector" type="select" label="Select a primer scheme from your history or use one from a tool data table?"
+                   help="Screening files must be stored in the 'primer_scheme_bedfiles' tool data table">
+                <option value="tool_data_table">From tool data table</option>
+                <option value="history">From history</option>
+            </param>
+            <when value="tool_data_table">
+                <param name="primer_scheme_bedfile" type="select" format="bed" label="Primer Scheme">
+	            <options from_data_table="primer_scheme_bedfiles">
+	                <validator type="no_options" message="No primer scheme .bed files are available" />
+                    </options>
+	        </param>
+            </when>
+            <when value="history">
+                <param name="primer_scheme_bedfile" type="data" format="bed" label="Primer Scheme" />
+            </when>
+        </conditional>
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Will you select a reference genome from your history or use a built-in reference?" >
+                <option value="cached">Use a built-in reference</option>
+                <option value="history">Use a reference from history</option>
+            </param>
+            <when value="cached">
+                <param name="reference" type="select" label="Using reference genome" help="Select genome from the list">
+                    <options from_data_table="all_fasta">
+                        <filter type="sort_by" column="2" />
+                        <validator type="no_options" message="No references are available" />
+                    </options>
+                </param>
+            </when>
+            <when value="history">
+                <param name="reference" type="data" format="fasta" label="Use the following dataset as the reference sequence"
+                       help="You can upload a FASTA sequence to the history and use it as reference" />
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data name="alignment_trimmed" format="bam" from_work_dir="*.trimmed.bam" label="${tool.name} on ${on_string}: trimmed alignment" />
+        <data name="variants_tabular" format="tabular" from_work_dir="*.variants.tab" label="${tool.name} on ${on_string}: variants table" />
+        <data name="variants_merged_vcf" format="vcf_bgzip" from_work_dir="*.merged.vcf.gz" label="${tool.name} on ${on_string}: variants vcf" />
+        <data name="consensus_fasta" format="fasta" from_work_dir="*.consensus.fasta" label="${tool.name} on ${on_string}: consensus sequence" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param name="read_file" value="SRR11410539_seqtk_sample_250_1.fastq" />
+            <param name="reference" value="nCoV-2019.reference.fasta" />
+            <param name="primer_scheme_source_selector" value="tool_data_table" />
+            <param name="primer_scheme_bedfile" value="test_entry" />
+            <output name="consensus_fasta" file="SRR11410539_seqtk_sample_250_1.fastq.consensus.fasta" />
+        </test>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param name="read_file" value="SRR11410539_seqtk_sample_250_1.fastq" />
+            <param name="reference" value="nCoV-2019.reference.fasta" />
+            <param name="primer_scheme_source_selector" value="history" />
+            <param name="primer_scheme_bedfile" value="nCoV-2019.V1.bed" />
+            <output name="consensus_fasta" file="SRR11410539_seqtk_sample_250_1.fastq.consensus.fasta" />
+        </test>
+        <test>
+            <param name="reference_source_selector" value="tool_data_table" />
+            <param name="read_file" value="SRR11410539_seqtk_sample_250_1.fastq" />
+            <param name="reference" value="test_entry" />
+            <param name="primer_scheme_source_selector" value="tool_data_table" />
+            <param name="primer_scheme_bedfile" value="test_entry" />
+            <output name="consensus_fasta" file="SRR11410539_seqtk_sample_250_1.fastq.consensus.fasta" />
+        </test>
+    </tests>
+    <help><![CDATA[
+This tool aligns MinION reads that were generated from a tiling amplicon library against a viral reference sequence. 
+It generates a consensus fasta file and a vcf variant file.
+
+This tool is configured to use the experimental 'medaka' variant caller.
+    ]]></help>
+    <citations>
+    </citations>
+</tool>