view test-data/in2.sam @ 0:c28c4c132459 draft default tip

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bamutil commit 9f971aa7d32d756aa4763ab29d1770178a5ece6b"
author iuc
date Fri, 02 Apr 2021 20:20:54 +0000
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@HD	VN:1.0	SO:coordinate
@SQ	SN:1	LN:248956422
@SQ	SN:2	LN:242193529
@SQ	SN:3	LN:198295559
@SQ	SN:4	LN:190214555
@SQ	SN:5	LN:181538259
@SQ	SN:6	LN:170805979
@SQ	SN:7	LN:159345973
@SQ	SN:8	LN:145138636
@SQ	SN:9	LN:138394717
@SQ	SN:10	LN:133797422
@SQ	SN:11	LN:135086622
@SQ	SN:12	LN:133275309
@SQ	SN:13	LN:114364328
@SQ	SN:14	LN:107043718
@SQ	SN:15	LN:101991189
@SQ	SN:16	LN:90338345
@SQ	SN:17	LN:83257441
@SQ	SN:18	LN:80373285
@SQ	SN:19	LN:58617616
@SQ	SN:20	LN:64444167
@SQ	SN:21	LN:46709983
@SQ	SN:22	LN:50818468
@SQ	SN:X	LN:156040895
@SQ	SN:Y	LN:57227415
@SQ	SN:MT	LN:16569
@PG	ID:hisat2	PN:hisat2	VN:2.1.0	CL:"/home/galaxy/galaxy/miniconda2/envs/mulled-v1-e7321ba46fa5ea4c6b9a06b78e6cd5182b33c0a47c2c86b5d610e1361f8b1686/bin/hisat2-align-s --wrapper basic-0 -p 4 -x /panfs/roc/rissdb/galaxy/genomes/GRCh38_canon/hisat2_index/GRCh38_canon/GRCh38_canon --pen-cansplice 0 --pen-noncansplice 12 --pen-canintronlen G,-8.0,1.0 --pen-noncanintronlen G,-8.0,1.0 --known-splicesite-infile splice_sites.txt --min-intronlen 20 --max-intronlen 500000 --dta -1 input_f.fastq -2 input_r.fastq"
@PG	ID:hisat2-55424A4	PN:hisat2	VN:2.1.0	CL:"/home/galaxy/galaxy/miniconda2/envs/mulled-v1-e7321ba46fa5ea4c6b9a06b78e6cd5182b33c0a47c2c86b5d610e1361f8b1686/bin/hisat2-align-s --wrapper basic-0 -p 4 -x /panfs/roc/rissdb/galaxy/genomes/GRCh38_canon/hisat2_index/GRCh38_canon/GRCh38_canon --pen-cansplice 0 --pen-noncansplice 12 --pen-canintronlen G,-8.0,1.0 --pen-noncanintronlen G,-8.0,1.0 --known-splicesite-infile splice_sites.txt --min-intronlen 20 --max-intronlen 500000 --dta -1 input_f.fastq -2 input_r.fastq"
@PG	ID:hisat2-55424A4-3A2CCEF5	PN:hisat2	VN:2.1.0	CL:"/home/galaxy/galaxy/miniconda2/envs/mulled-v1-e7321ba46fa5ea4c6b9a06b78e6cd5182b33c0a47c2c86b5d610e1361f8b1686/bin/hisat2-align-s --wrapper basic-0 -p 4 -x /panfs/roc/rissdb/galaxy/genomes/GRCh38_canon/hisat2_index/GRCh38_canon/GRCh38_canon --pen-cansplice 0 --pen-noncansplice 12 --pen-canintronlen G,-8.0,1.0 --pen-noncanintronlen G,-8.0,1.0 --known-splicesite-infile splice_sites.txt --min-intronlen 20 --max-intronlen 500000 --dta -1 input_f.fastq -2 input_r.fastq"
@PG	ID:samtools	PN:samtools	PP:hisat2-55424A4-3A2CCEF5	VN:1.11	CL:samtools sort -@ 1 -m 10240M -T sorttemp -O sam -o 0.sam /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075445.dat
@PG	ID:hisat2-3A2CCEF5	PN:hisat2	VN:2.1.0	CL:"/home/galaxy/galaxy/miniconda2/envs/mulled-v1-e7321ba46fa5ea4c6b9a06b78e6cd5182b33c0a47c2c86b5d610e1361f8b1686/bin/hisat2-align-s --wrapper basic-0 -p 4 -x /panfs/roc/rissdb/galaxy/genomes/GRCh38_canon/hisat2_index/GRCh38_canon/GRCh38_canon --pen-cansplice 0 --pen-noncansplice 12 --pen-canintronlen G,-8.0,1.0 --pen-noncanintronlen G,-8.0,1.0 --known-splicesite-infile splice_sites.txt --min-intronlen 20 --max-intronlen 500000 --dta -1 input_f.fastq -2 input_r.fastq"
@PG	ID:samtools-6ADB4A65	PN:samtools	PP:hisat2-3A2CCEF5	VN:1.11	CL:samtools sort -@ 1 -m 10240M -T sorttemp -O sam -o 1.sam /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075446.dat
@PG	ID:samtools.1	PN:samtools	PP:samtools	VN:1.11	CL:samtools merge -@ 1 -s 1 -f -h /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075446.dat /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075449.dat 0.sam 1.sam
@PG	ID:samtools.2	PN:samtools	PP:samtools-6ADB4A65	VN:1.11	CL:samtools merge -@ 1 -s 1 -f -h /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075446.dat /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075449.dat 0.sam 1.sam
@PG	ID:hisat2-6ADB4A65	PN:hisat2	VN:2.1.0	CL:"/home/galaxy/galaxy/miniconda2/envs/mulled-v1-e7321ba46fa5ea4c6b9a06b78e6cd5182b33c0a47c2c86b5d610e1361f8b1686/bin/hisat2-align-s --wrapper basic-0 -p 4 -x /panfs/roc/rissdb/galaxy/genomes/GRCh38_canon/hisat2_index/GRCh38_canon/GRCh38_canon --pen-cansplice 0 --pen-noncansplice 12 --pen-canintronlen G,-8.0,1.0 --pen-noncanintronlen G,-8.0,1.0 --known-splicesite-infile splice_sites.txt --min-intronlen 20 --max-intronlen 500000 --dta -1 input_f.fastq -2 input_r.fastq"
@PG	ID:samtools.3	PN:samtools	PP:samtools.1	VN:1.11	CL:samtools merge -@ 1 -s 1 -f -h /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075453.dat /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075522.dat 0.sam 1.sam
@PG	ID:samtools.4	PN:samtools	PP:samtools.2	VN:1.11	CL:samtools merge -@ 1 -s 1 -f -h /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075453.dat /panfs/roc/galaxy/PRODUCTION/database/files/001/075/dataset_1075522.dat 0.sam 1.sam
NB500964:249:HHLFNBGX7:4:12608:21020:10228	163	6	52995635	1	75M	=	52995715	155	CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCC	AAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEAEEEE<EEE//<AAEAAEEEE<AAE/EEEEAE	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-11	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP
NB500964:249:HHLFNBGX7:4:23603:22568:16607	163	6	52995635	1	75M	=	52995715	155	CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCC	AAAAAEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEA/EAEEAAAEEEEE	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-11	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP
NB500964:249:HHLFNBGX7:4:12608:21020:10228	419	6	52995635	1	75M	=	52995715	155	CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCC	AAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEAEEEE<EEE//<AAEAAEEEE<AAE/EEEEAE	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:75M	ZF:i:163	ZM:i:1	ZN:Z:6:52995715	ZI:i:155	ZS:i:-11	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP	ZP:Z:6:52995635	ZS:Z:CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCC	ZQ:Z:AAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEAEEEE<EEE//<AAEAAEEEE<AAE/EEEEAE	ZT:Z:AS:i:0;MD:Z:75;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-11;XO:i:0;XG:i:0;YS:i:-5;YT:Z:CP
NB500964:249:HHLFNBGX7:4:23603:22568:16607	419	6	52995635	1	75M	=	52995715	155	CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCC	AAAAAEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEA/EAEEAAAEEEEE	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:75M	ZF:i:163	ZM:i:1	ZN:Z:6:52995715	ZI:i:155	ZS:i:-11	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP	ZP:Z:6:52995635	ZS:Z:CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCC	ZQ:Z:AAAAAEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEA/EAEEAAAEEEEE	ZT:Z:AS:i:0;MD:Z:75;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-11;XO:i:0;XG:i:0;YS:i:-5;YT:Z:CP
NB500964:249:HHLFNBGX7:4:11510:10074:3541	163	6	52995646	60	75M	=	52995715	144	ATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA	AAAAAEE/EEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEAEE/	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:4:12404:22663:7685	163	6	52995648	1	75M	=	52995715	142	CTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCATCACC	AAAAAEEE//EEEEEE/EAEEEEEEEEEE/AEE/EEEEEEEEEEEEAEAEEA/AEEAEEE<<EE/EEEE/EEAEE	AS:i:-3	MD:Z:69C5	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-3	XO:i:0	XG:i:0	YS:i:-6	YT:Z:CP
NB500964:249:HHLFNBGX7:4:12404:22663:7685	419	6	52995648	1	75M	=	52995715	142	CTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCATCACC	AAAAAEEE//EEEEEE/EAEEEEEEEEEE/AEE/EEEEEEEEEEEEAEAEEA/AEEAEEE<<EE/EEEE/EEAEE	AS:i:-3	MD:Z:69C5	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:75M	ZF:i:163	ZM:i:1	ZN:Z:6:52995715	ZI:i:142	ZS:i:-3	XO:i:0	XG:i:0	YS:i:-6	YT:Z:CP	ZP:Z:6:52995648	ZS:Z:CTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCATCACC	ZQ:Z:AAAAAEEE//EEEEEE/EAEEEEEEEEEE/AEE/EEEEEEEEEEEEAEAEEA/AEEAEEE<<EE/EEEE/EEAEE	ZT:Z:AS:i:-3;MD:Z:69C5;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-3;XO:i:0;XG:i:0;YS:i:-6;YT:Z:CP
NB500964:249:HHLFNBGX7:2:13209:7691:13203	163	6	52995675	60	75M	=	52995789	189	ATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTG	AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEEEAEEEEE	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:21407:1974:9687	163	6	52995709	1	74M66N1M	=	52995832	132	CTTCCTCCCTCACCGCTCCATGTCCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCACCGATA	A//AAAE/EE//EE/EEEAAEA//EEEEE/AEEEEEEEEEEEE/E/E6/A/E//A/E/EEEA/AAEAE//EEA/A	AS:i:-6	MD:Z:23G44C6	NM:i:2	NH:i:2	XN:i:0	XM:i:2	ZS:i:-6	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:3:22501:2652:1890	163	6	52995709	1	74M74N1M	=	52995788	80	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCACGATA	AAAAAE/EEEA/EAAEEEEEEEEEEEE/<EEEEAA<EEEE/6EEEEEEEAEEEAEAAE/EEEAEE<EAE/EEE<A	AS:i:-3	MD:Z:69C5	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-3	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:3:22503:25677:1737	163	6	52995709	1	74M66N1M	=	52995785	85	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATACCCGATA	AAAA/E/AAEAEE/EE/AAAEEE/EEEEEEEEAEAEEEEEAEEEEEAEE/EE//EEE/AEE/AE//E/E/EA/E/	AS:i:-3	MD:Z:67C7	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-3	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP
NB500964:249:HHLFNBGX7:3:22611:13268:8039	163	6	52995709	1	74M66N1M	=	52995848	148	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCACCGATA	AAAAAEEAEAEEEA/EEEEAAE/AEE/<AEEEE/EEEEAEEE<AAEEAEEEAEEEEEEEEE/AEAEAE/EEAEE/	AS:i:-3	MD:Z:68C6	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-3	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP
NB500964:249:HHLFNBGX7:4:13607:25752:7238	163	6	52995709	1	74M74N1M	=	52995789	81	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCCATA	AAAAA/EEE/EEEEEEEEEAE/A6AEEEEEEEEEEE/<EEEEAEAEEEE/EEE/EEE/EAEE/AAE/EEEE///A	AS:i:-3	MD:Z:71G3	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-3	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:4:21503:16243:7076	163	6	52995709	1	74M66N1M	=	52995838	138	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATACCCGATA	AAAA/EEE/EE/AEAEA/EEAA/AAE/EEE/EAEEA/AEEEE<EEEE<<//E/AE/E6EE/</EAAE/<EE/EEE	AS:i:-3	MD:Z:67C7	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-3	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:3:21407:1974:9687	419	6	52995709	1	74M74N1M	=	52995832	124	CTTCCTCCCTCACCGCTCCATGTCCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCACCGATA	A//AAAE/EE//EE/EEEAAEA//EEEEE/AEEEEEEEEEEEE/E/E6/A/E//A/E/EEEA/AAEAE//EEA/A	AS:i:-6	MD:Z:23G44C6	NM:i:2	NH:i:2	XN:i:0	XM:i:2	ZC:Z:74M66N1M	ZF:i:163	ZM:i:1	ZN:Z:6:52995832	ZI:i:132	ZS:i:-6	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995709	ZS:Z:CTTCCTCCCTCACCGCTCCATGTCCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCACCGATA	ZQ:Z:A//AAAE/EE//EE/EEEAAEA//EEEEE/AEEEEEEEEEEEE/E/E6/A/E//A/E/EEEA/AAEAE//EEA/A	ZT:Z:AS:i:-6;MD:Z:23G44C6;NM:i:2;NH:i:2;XN:i:0;XM:i:2;ZS:i:-6;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:3:22501:2652:1890	419	6	52995709	1	74M66N1M	=	52995788	88	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCACGATA	AAAAAE/EEEA/EAAEEEEEEEEEEEE/<EEEEAA<EEEE/6EEEEEEEAEEEAEAAE/EEEAEE<EAE/EEE<A	AS:i:-3	MD:Z:69C5	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:74M74N1M	ZF:i:163	ZM:i:1	ZN:Z:6:52995788	ZI:i:80	ZS:i:-3	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995709	ZS:Z:CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCACGATA	ZQ:Z:AAAAAE/EEEA/EAAEEEEEEEEEEEE/<EEEEAA<EEEE/6EEEEEEEAEEEAEAAE/EEEAEE<EAE/EEE<A	ZT:Z:AS:i:-3;MD:Z:69C5;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-3;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:3:22503:25677:1737	419	6	52995709	1	74M74N1M	=	52995785	77	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATACCCGATA	AAAA/E/AAEAEE/EE/AAAEEE/EEEEEEEEAEAEEEEEAEEEEEAEE/EE//EEE/AEE/AE//E/E/EA/E/	AS:i:-3	MD:Z:67C7	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:74M66N1M	ZF:i:163	ZM:i:1	ZN:Z:6:52995785	ZI:i:85	ZS:i:-3	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP	ZP:Z:6:52995709	ZS:Z:CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATACCCGATA	ZQ:Z:AAAA/E/AAEAEE/EE/AAAEEE/EEEEEEEEAEAEEEEEAEEEEEAEE/EE//EEE/AEE/AE//E/E/EA/E/	ZT:Z:AS:i:-3;MD:Z:67C7;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-3;XO:i:0;XG:i:0;YS:i:-5;YT:Z:CP
NB500964:249:HHLFNBGX7:3:22611:13268:8039	419	6	52995709	1	74M74N1M	=	52995848	140	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCACCGATA	AAAAAEEAEAEEEA/EEEEAAE/AEE/<AEEEE/EEEEAEEE<AAEEAEEEAEEEEEEEEE/AEAEAE/EEAEE/	AS:i:-3	MD:Z:68C6	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:74M66N1M	ZF:i:163	ZM:i:1	ZN:Z:6:52995848	ZI:i:148	ZS:i:-3	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP	ZP:Z:6:52995709	ZS:Z:CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCACCGATA	ZQ:Z:AAAAAEEAEAEEEA/EEEEAAE/AEE/<AEEEE/EEEEAEEE<AAEEAEEEAEEEEEEEEE/AEAEAE/EEAEE/	ZT:Z:AS:i:-3;MD:Z:68C6;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-3;XO:i:0;XG:i:0;YS:i:-3;YT:Z:CP
NB500964:249:HHLFNBGX7:4:13607:25752:7238	419	6	52995709	1	74M66N1M	=	52995789	89	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCCATA	AAAAA/EEE/EEEEEEEEEAE/A6AEEEEEEEEEEE/<EEEEAEAEEEE/EEE/EEE/EAEE/AAE/EEEE///A	AS:i:-3	MD:Z:71G3	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:74M74N1M	ZF:i:163	ZM:i:1	ZN:Z:6:52995789	ZI:i:81	ZS:i:-3	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995709	ZS:Z:CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCCATA	ZQ:Z:AAAAA/EEE/EEEEEEEEEAE/A6AEEEEEEEEEEE/<EEEEAEAEEEE/EEE/EEE/EAEE/AAE/EEEE///A	ZT:Z:AS:i:-3;MD:Z:71G3;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-3;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:4:21503:16243:7076	419	6	52995709	1	74M74N1M	=	52995838	130	CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATACCCGATA	AAAA/EEE/EE/AEAEA/EEAA/AAE/EEE/EAEEA/AEEEE<EEEE<<//E/AE/E6EE/</EAAE/<EE/EEE	AS:i:-3	MD:Z:67C7	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:74M66N1M	ZF:i:163	ZM:i:1	ZN:Z:6:52995838	ZI:i:138	ZS:i:-3	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995709	ZS:Z:CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATACCCGATA	ZQ:Z:AAAA/EEE/EE/AEAEA/EEAA/AAE/EEE/EAEEA/AEEEE<EEEE<<//E/AE/E6EE/</EAAE/<EE/EEE	ZT:Z:AS:i:-3;MD:Z:67C7;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-3;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:3:13604:7137:3437	83	6	52995715	1	68M74N2M5S	=	52995637	-153	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	<EEEEEEEEEEEEEE<EEEE6EEEE<E/EEEEEAEEEEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:3:23501:15949:4416	83	6	52995715	1	68M74N2M5S	=	52995636	-154	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	EEEEEEEEEEEEEEEAEEAEEEEEEEEEEEEEAEEEEEEEEAAEEEAEEEEAEEEEEEEEAEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:4:12404:22663:7685	83	6	52995715	1	68M74N1M6S	=	52995648	-142	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATACAGGAG	</EEA/EEA/EEA/EEEEAEEEEE/EEAEEEEEEEAE<//EEAEE/EEEEEEAE<AE/6EEE/EEEEEAEAAAAA	AS:i:-6	MD:Z:69	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-5	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP
NB500964:249:HHLFNBGX7:4:12608:21020:10228	83	6	52995715	1	68M66N2M5S	=	52995635	-155	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	AEEEEEEA<AEEAEEEEE<<EEEEEEEAAEAEEEEEAEEEEEE/EEEEEAEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:4:23603:22568:16607	83	6	52995715	1	68M74N2M5S	=	52995635	-155	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP
NB500964:249:HHLFNBGX7:3:13604:7137:3437	339	6	52995715	1	68M66N2M5S	=	52995637	-153	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	<EEEEEEEEEEEEEE<EEEE6EEEE<E/EEEEEAEEEEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:68M74N2M5S	ZF:i:83	ZM:i:1	ZN:Z:6:52995637	ZI:i:-153	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995715	ZS:Z:CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	ZQ:Z:<EEEEEEEEEEEEEE<EEEE6EEEE<E/EEEEEAEEEEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEAAAAA	ZT:Z:AS:i:-5;MD:Z:70;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-5;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:3:23501:15949:4416	339	6	52995715	1	68M66N2M5S	=	52995636	-154	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	EEEEEEEEEEEEEEEAEEAEEEEEEEEEEEEEAEEEEEEEEAAEEEAEEEEAEEEEEEEEAEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:68M74N2M5S	ZF:i:83	ZM:i:1	ZN:Z:6:52995636	ZI:i:-154	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995715	ZS:Z:CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	ZQ:Z:EEEEEEEEEEEEEEEAEEAEEEEEEEEEEEEEAEEEEEEEEAAEEEAEEEEAEEEEEEEEAEEEEEEEEEAAAAA	ZT:Z:AS:i:-5;MD:Z:70;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-5;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:4:12404:22663:7685	339	6	52995715	1	68M66N1M6S	=	52995648	-142	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATACAGGAG	</EEA/EEA/EEA/EEEEAEEEEE/EEAEEEEEEEAE<//EEAEE/EEEEEEAE<AE/6EEE/EEEEEAEAAAAA	AS:i:-6	MD:Z:69	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:68M74N1M6S	ZF:i:83	ZM:i:1	ZN:Z:6:52995648	ZI:i:-142	ZS:i:-5	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP	ZP:Z:6:52995715	ZS:Z:CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATACAGGAG	ZQ:Z:</EEA/EEA/EEA/EEEEAEEEEE/EEAEEEEEEEAE<//EEAEE/EEEEEEAE<AE/6EEE/EEEEEAEAAAAA	ZT:Z:AS:i:-6;MD:Z:69;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-5;XO:i:0;XG:i:0;YS:i:-3;YT:Z:CP
NB500964:249:HHLFNBGX7:4:12608:21020:10228	339	6	52995715	1	68M74N2M5S	=	52995635	-155	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	AEEEEEEA<AEEAEEEEE<<EEEEEEEAAEAEEEEEAEEEEEE/EEEEEAEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:68M66N2M5S	ZF:i:83	ZM:i:1	ZN:Z:6:52995635	ZI:i:-155	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995715	ZS:Z:CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	ZQ:Z:AEEEEEEA<AEEAEEEEE<<EEEEEEEAAEAEEEEEAEEEEEE/EEEEEAEEEEEEEEEEEEEEEEEEEEAAAAA	ZT:Z:AS:i:-5;MD:Z:70;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-5;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:4:23603:22568:16607	339	6	52995715	1	68M66N2M5S	=	52995635	-155	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:68M74N2M5S	ZF:i:83	ZM:i:1	ZN:Z:6:52995635	ZI:i:-155	ZS:i:-5	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	ZP:Z:6:52995715	ZS:Z:CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAAAGGAG	ZQ:Z:EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	ZT:Z:AS:i:-5;MD:Z:70;NM:i:0;NH:i:2;XN:i:0;XM:i:0;ZS:i:-5;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP
NB500964:249:HHLFNBGX7:4:11510:10074:3541	83	6	52995715	60	75M	=	52995646	-144	CCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG	EEEAEEE/EAEAEEEAEEE<EAEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:21512:8039:1524	163	6	52995730	60	75M	=	52995836	181	GTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTC	AAAAAEEAEEEEEEEEEEEEEAE/EEE/EEEEEEEAEEEEE/EEEEEEEEEE/EAEEEEAEEEEEAEAEAA/EEE	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:23508:17777:5737	163	6	52995769	0	75M	=	52995832	138	CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGATCCTC	AAAAAEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAE	AS:i:-5	MD:Z:70A4	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-5	XO:i:0	XG:i:0	YS:i:-10	YT:Z:CP
NB500964:249:HHLFNBGX7:3:23508:17777:5737	419	6	52995769	0	70M5S	=	52995832	138	CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGATCCTC	AAAAAEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAE	AS:i:-5	MD:Z:70	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZC:Z:75M	ZF:i:163	ZM:i:0	ZN:Z:6:52995832	ZI:i:138	ZS:i:-5	XO:i:0	XG:i:0	YS:i:-10	YT:Z:CP	ZP:Z:6:52995769	ZS:Z:CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGATCCTC	ZQ:Z:AAAAAEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAE	ZT:Z:AS:i:-5;MD:Z:70A4;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-5;XO:i:0;XG:i:0;YS:i:-10;YT:Z:CP
NB500964:249:HHLFNBGX7:3:22503:25677:1737	83	6	52995785	1	74M1S	=	52995709	-85	AGGAGGACCGGGCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAC	EA<E//EEA</6EAEAEAEEAEE/EEE6EEEEEEEAEAEE/EEEEEEA/EEEAEEEEEEEEEE6EE/AEE/AAA/	AS:i:-5	MD:Z:11T62	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-5	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP
NB500964:249:HHLFNBGX7:3:22503:25677:1737	339	6	52995785	1	74M1S	=	52995709	-77	AGGAGGACCGGGCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAC	EA<E//EEA</6EAEAEAEEAEE/EEE6EEEEEEEAEAEE/EEEEEEA/EEEAEEEEEEEEEE6EE/AEE/AAA/	AS:i:-5	MD:Z:11T62	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZC:Z:74M1S	ZF:i:83	ZM:i:1	ZN:Z:6:52995709	ZI:i:-85	ZS:i:-5	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP	ZP:Z:6:52995785	ZS:Z:AGGAGGACCGGGCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAC	ZQ:Z:EA<E//EEA</6EAEAEAEEAEE/EEE6EEEEEEEAEAEE/EEEEEEA/EEEAEEEEEEEEEE6EE/AEE/AAA/	ZT:Z:AS:i:-5;MD:Z:11T62;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-5;XO:i:0;XG:i:0;YS:i:-3;YT:Z:CP
NB500964:249:HHLFNBGX7:2:13209:7691:13203	83	6	52995789	60	75M	=	52995675	-189	GGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCC	EAAAA<<AEEAEEEEEAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:4:21507:2226:9226	163	6	52995797	60	75M	=	52995834	112	CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAG	AAAAAEEEEEEEEEEE/EEAEEEEEEEE/EEEEE/EEEEEAA//EEEE/E/EE/EEEE/E<E/AEE/6EEEEE/E	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:-3	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:4:21602:10644:5676	163	6	52995802	60	75M	=	52995834	107	GTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAA	AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE	AS:i:0	ZS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:21407:1974:9687	83	6	52995832	1	75M	=	52995709	-132	TGCTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAG	EEEEEEE/EEE<EEE/EEAEEEE/A</EEEE6EEAE/EEEAEEEEEEEEEEEEEA6EEEEEEE/EAEEEEAAAA<	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	XO:i:0	XG:i:0	YS:i:-6	YT:Z:CP
NB500964:249:HHLFNBGX7:3:23508:17777:5737	83	6	52995832	0	70M5S	=	52995769	-138	TGCTAGATCCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACCCCAG	EEEEEAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-10	MD:Z:7A62	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZS:i:-10	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP
NB500964:249:HHLFNBGX7:3:21407:1974:9687	339	6	52995832	1	75M	=	52995709	-124	TGCTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAG	EEEEEEE/EEE<EEE/EEAEEEE/A</EEEE6EEAE/EEEAEEEEEEEEEEEEEA6EEEEEEE/EAEEEEAAAA<	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZF:i:83	ZM:i:1	ZN:Z:6:52995709	ZI:i:-132	ZS:Z:TGCTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAG	XO:i:0	XG:i:0	YS:i:-6	YT:Z:CP	ZP:Z:6:52995832	ZC:Z:75M	ZQ:Z:EEEEEEE/EEE<EEE/EEAEEEE/A</EEEE6EEAE/EEEAEEEEEEEEEEEEEA6EEEEEEE/EAEEEEAAAA<	ZT:Z:AS:i:0;MD:Z:75;NM:i:0;NH:i:2;XN:i:0;XM:i:0;XO:i:0;XG:i:0;YS:i:-6;YT:Z:CP
NB500964:249:HHLFNBGX7:3:23508:17777:5737	339	6	52995832	0	75M	=	52995769	-138	TGCTAGATCCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACCCCAG	EEEEEAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	AS:i:-10	MD:Z:7A62T4	NM:i:2	NH:i:2	XN:i:0	XM:i:2	ZC:Z:70M5S	ZF:i:83	ZM:i:0	ZN:Z:6:52995769	ZI:i:-138	ZS:i:-10	XO:i:0	XG:i:0	YS:i:-5	YT:Z:CP	ZP:Z:6:52995832	ZS:Z:TGCTAGATCCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACCCCAG	ZQ:Z:EEEEEAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA	ZT:Z:AS:i:-10;MD:Z:7A62;NM:i:1;NH:i:2;XN:i:0;XM:i:1;ZS:i:-10;XO:i:0;XG:i:0;YS:i:-5;YT:Z:CP
NB500964:249:HHLFNBGX7:3:12605:20423:18895	83	6	52995834	1	75M	=	52995719	-116	CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGAC	/EEEEAEAEAEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEAEEEAAAAA	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	XO:i:0	XG:i:0	YS:i:-8	YT:Z:CP
NB500964:249:HHLFNBGX7:3:21607:9911:11221	83	6	52995834	0	75M	=	52995717	-118	CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAAGGTAGTCAAGCTTCCAAGACTCCAGAC	EEEEEAEAEEEEEEEE</E/EEEE/E<A//EE6EEE/EEEEEEEEEEEEEEEE/A//EEEEEEEEEA/EEAAAAA	AS:i:-5	MD:Z:47G27	NM:i:1	NH:i:2	XN:i:0	XM:i:1	XO:i:0	XG:i:0	YS:i:-8	YT:Z:CP
NB500964:249:HHLFNBGX7:3:12605:20423:18895	339	6	52995834	1	75M	=	52995719	-124	CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGAC	/EEEEAEAEAEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEAEEEAAAAA	AS:i:0	MD:Z:75	NM:i:0	NH:i:2	XN:i:0	XM:i:0	ZF:i:83	ZM:i:1	ZN:Z:6:52995719	ZI:i:-116	ZS:Z:CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGAC	XO:i:0	XG:i:0	YS:i:-8	YT:Z:CP	ZP:Z:6:52995834	ZC:Z:75M	ZQ:Z:/EEEEAEAEAEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEAEEEAAAAA	ZT:Z:AS:i:0;MD:Z:75;NM:i:0;NH:i:2;XN:i:0;XM:i:0;XO:i:0;XG:i:0;YS:i:-8;YT:Z:CP
NB500964:249:HHLFNBGX7:3:21607:9911:11221	339	6	52995834	0	75M	=	52995717	-126	CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAAGGTAGTCAAGCTTCCAAGACTCCAGAC	EEEEEAEAEEEEEEEE</E/EEEE/E<A//EE6EEE/EEEEEEEEEEEEEEEE/A//EEEEEEEEEA/EEAAAAA	AS:i:-5	MD:Z:47G27	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZF:i:83	ZM:i:0	ZN:Z:6:52995717	ZI:i:-118	ZS:Z:CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAAGGTAGTCAAGCTTCCAAGACTCCAGAC	XO:i:0	XG:i:0	YS:i:-8	YT:Z:CP	ZP:Z:6:52995834	ZC:Z:75M	ZQ:Z:EEEEEAEAEEEEEEEE</E/EEEE/E<A//EE6EEE/EEEEEEEEEEEEEEEE/A//EEEEEEEEEA/EEAAAAA	ZT:Z:AS:i:-5;MD:Z:47G27;NM:i:1;NH:i:2;XN:i:0;XM:i:1;XO:i:0;XG:i:0;YS:i:-8;YT:Z:CP
NB500964:249:HHLFNBGX7:4:21507:2226:9226	83	6	52995834	60	75M	=	52995797	-112	CTAGAACCTCCAAACAACCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGAC	AEEEEE<EEEEEEEAEA/<A//EEEEEEEEEEEAEE/EEAAE/EAE/EE</AE/EEEEAEEEEEEEEEEEAAAAA	AS:i:-3	XN:i:0	XM:i:1	XO:i:0	XG:i:0	NM:i:1	MD:Z:17G57	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:4:21602:10644:5676	83	6	52995834	60	75M	=	52995802	-107	CTAGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGAC	EEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6AAAA	AS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:21512:8039:1524	83	6	52995836	60	75M	=	52995730	-181	AGAACCTCCAAACAAGCTCTCAAGGTCCATTTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGACAC	/EA/EEEEAE/E/A</E/E/6/EEEEEEEE/EEEEE/EEEEEAEEEEEEEE6EEEEAE6EEEEEEEEEEEAAAAA	AS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:22611:13268:8039	83	6	52995848	1	75M	=	52995709	-148	CAAGCTCTCAAGGTCCATTTGTAGGATAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGACACATCCAAATGAGG	AE/EE/<EE//AEA<AEEEEEEEE6E/EEAEEEEAE6EE/EE///EE//EE<EEEAEAAEAAE/EEAEEEAAA6A	AS:i:-3	MD:Z:26G48	NM:i:1	NH:i:2	XN:i:0	XM:i:1	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP
NB500964:249:HHLFNBGX7:3:22611:13268:8039	339	6	52995848	1	75M	=	52995709	-140	CAAGCTCTCAAGGTCCATTTGTAGGATAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGACACATCCAAATGAGG	AE/EE/<EE//AEA<AEEEEEEEE6E/EEAEEEEAE6EE/EE///EE//EE<EEEAEAAEAAE/EEAEEEAAA6A	AS:i:-3	MD:Z:26G48	NM:i:1	NH:i:2	XN:i:0	XM:i:1	ZF:i:83	ZM:i:1	ZN:Z:6:52995709	ZI:i:-148	ZS:Z:CAAGCTCTCAAGGTCCATTTGTAGGATAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGACACATCCAAATGAGG	XO:i:0	XG:i:0	YS:i:-3	YT:Z:CP	ZP:Z:6:52995848	ZC:Z:75M	ZQ:Z:AE/EE/<EE//AEA<AEEEEEEEE6E/EEAEEEEAE6EE/EE///EE//EE<EEEAEAAEAAE/EEAEEEAAA6A	ZT:Z:AS:i:-3;MD:Z:26G48;NM:i:1;NH:i:2;XN:i:0;XM:i:1;XO:i:0;XG:i:0;YS:i:-3;YT:Z:CP
NB500964:249:HHLFNBGX7:3:13604:19654:15542	99	6	53004731	60	75M	=	53004936	280	CTTAAATTGCACCCCTGTTGCAATACTGCATTAATATTATTACATTTTTCAAAATCTGTAGAAAATATTATCTGG	AAAAAEEEEEEEA//EEEEEEEEEEEEEEAEEEEEEEEEEEEAEE/EEEEEEEEEEEEEEEEE/EEEEEEE/EEE	AS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:3:13604:19654:15542	147	6	53004936	60	75M	=	53004731	-280	AATAATTTTATACAAAAAAGCCTACTGCATTCAAATCAATATTTTAAAAAAAAACTTTAAAAAAGAATATTGACA	EEEEEAEEEEEEEEEEEEEAEE/EAEEEEEEEEEEEEEEEEEEEEEEEEEEAEAAEEAEEEEAAEEEEEEAAAAA	AS:i:0	XN:i:0	XM:i:0	XO:i:0	XG:i:0	NM:i:0	MD:Z:75	YS:i:0	YT:Z:CP	NH:i:1
NB500964:249:HHLFNBGX7:4:13509:13430:12762	99	6	73519451	1	66M366N9M	=	73519983	241	ACCACGTTCACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGC	AAAAAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEAEEEEEEEEEEE/EE/EEEEEAEEAEEEAAEEEEEEEE<E<	AS:i:0	MD:Z:75	NM:i:0	NH:i:3	XN:i:0	XM:i:0	ZS:i:0	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	XS:A:-
NB500964:249:HHLFNBGX7:4:13509:13430:12762	355	6	73519451	1	66M366N9M	=	73519983	241	ACCACGTTCACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGC	AAAAAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEAEEEEEEEEEEE/EE/EEEEEAEEAEEEAAEEEEEEEE<E<	AS:i:0	MD:Z:75	NM:i:0	NH:i:3	XN:i:0	XM:i:0	ZS:i:0	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	XS:A:-
NB500964:249:HHLFNBGX7:4:13509:13430:12762	355	6	73519451	1	66M366N9M	=	73519983	241	ACCACGTTCACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGC	AAAAAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEAEEEEEEEEEEE/EE/EEEEEAEEAEEEAAEEEEEEEE<E<	AS:i:0	MD:Z:75	NM:i:0	NH:i:3	XN:i:0	XM:i:0	ZP:Z:6:73519451	ZC:Z:66M366N9M	ZF:i:99	ZM:i:1	ZN:Z:6:73519983	ZS:i:0	XO:i:0	XG:i:0	YS:i:0	YT:Z:CP	XS:A:-	ZI:i:241	ZS:Z:ACCACGTTCACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGC	ZQ:Z:AAAAAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEAEEEEEEEEEEE/EE/EEEEEAEEAEEEAAEEEEEEEE<E<	ZT:Z:AS:i:0;MD:Z:75;NM:i:0;NH:i:3;XN:i:0;XM:i:0;ZS:i:0;XO:i:0;XG:i:0;YS:i:0;YT:Z:CP;XS:A:-
NB500964:249:HHLFNBGX7:3:12402:8344:2452	99	6	73519463	60	54M366N21M	=	73519916	162	CTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCGCATCTCAGCAGCCTCCTTCTCAAA	A6AAAEE6EEE6/EEEE/<EEEE/<EEEEEEEEEEEE//EEE/E<E/////<E/A/AA///AAEEE<//E</</A	AS:i:-3	ZS:i:-3	XN:i:0	XM:i:1	XO:i:0	XG:i:0	NM:i:1	MD:Z:50C24	YS:i:-9	YT:Z:CP	XS:A:-	NH:i:1
NB500964:249:HHLFNBGX7:3:12402:8344:2452	147	6	73519916	60	75M	=	73519463	-162	TCTTTTGGCGATGCCACCGCATTTATAGATCAGATGGCCAGTAGTGGTGGACTTGCCCGAAGCTACGGGTCCAAT	/EEAE6</EE//EEE//AEEEA/EE/<//AE/EE/E//AEAA/EAAAA/AE6EE///E//A/EAE6E/EE/AAAA	AS:i:-9	ZS:i:-9	XN:i:0	XM:i:3	XO:i:0	XG:i:0	NM:i:3	MD:Z:7T53T5T7	YS:i:-3	YT:Z:CP	NH:i:1