Mercurial > repos > iuc > bbtools_bbmerge
comparison bbmerge.xml @ 6:cab19c0452c4 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit cd59ba2c349865259b92302a1d70e103b8a5e3cb
author | iuc |
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date | Tue, 27 Aug 2024 10:15:19 +0000 |
parents | 1af48f9cc27b |
children |
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5:1af48f9cc27b | 6:cab19c0452c4 |
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96 | 96 |
97 $merge_options.strictness=t | 97 $merge_options.strictness=t |
98 ]]></command> | 98 ]]></command> |
99 <inputs> | 99 <inputs> |
100 <expand macro="input_type_cond"/> | 100 <expand macro="input_type_cond"/> |
101 | |
102 <section name="qt_options" title="Quality and trimming options"> | 101 <section name="qt_options" title="Quality and trimming options"> |
103 <param name="qtrim" type="select" label="Select option for quality trimming ends before mapping"> | 102 <param name="qtrim" type="select" label="Select option for quality trimming ends before mapping"> |
104 <option value="f" selected="true">No trimming</option> | 103 <option value="f" selected="true">No trimming</option> |
105 <option value="l">Trim left</option> | 104 <option value="l">Trim left</option> |
106 <option value="r">Trim right</option> | 105 <option value="r">Trim right</option> |
108 </param> | 107 </param> |
109 <param argument="trimq" type="integer" value="6" label="Trim regions with average quality below this value"/> | 108 <param argument="trimq" type="integer" value="6" label="Trim regions with average quality below this value"/> |
110 <param argument="minlength_after_trim" type="integer" value="60" label="Don't trim reads to be shorter than this value"/> | 109 <param argument="minlength_after_trim" type="integer" value="60" label="Don't trim reads to be shorter than this value"/> |
111 <param argument="usequality" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Use quality scores when determining which read kmers to use as seeds?"/> | 110 <param argument="usequality" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Use quality scores when determining which read kmers to use as seeds?"/> |
112 </section> | 111 </section> |
113 | |
114 <section name="merge_options" title="Merging parameters"> | 112 <section name="merge_options" title="Merging parameters"> |
115 <param name="strictness" type="select" label="Select option for quality trimming ends before mapping"> | 113 <param name="strictness" type="select" label="Select option for quality trimming ends before mapping"> |
116 <option value="xstrict">max strict</option> | 114 <option value="xstrict">max strict</option> |
117 <option value="ustrict">ultra strict</option> | 115 <option value="ustrict">ultra strict</option> |
118 <option value="vstrict">very strict</option> | 116 <option value="vstrict">very strict</option> |
126 </param> | 124 </param> |
127 <param argument="ecco" type="boolean" truevalue="t" falsevalue="f" checked="false" label="Error-correct the overlapping part, but don't merge." help="If selected, the tool with find the overlaps as if merging reads, use this overlap information to correct sequencing errors on both strands. However, the strands from the mates will not be merged but provided as two separate reads after error correction."/> | 125 <param argument="ecco" type="boolean" truevalue="t" falsevalue="f" checked="false" label="Error-correct the overlapping part, but don't merge." help="If selected, the tool with find the overlaps as if merging reads, use this overlap information to correct sequencing errors on both strands. However, the strands from the mates will not be merged but provided as two separate reads after error correction."/> |
128 <param argument="trimnonoverlapping" type="boolean" truevalue="t" falsevalue="f" checked="false" label="Trim all non-overlapping portions, leaving only consensus sequence. By default, only sequence to the right of the overlap (adapter sequence) is trimmed."/> | 126 <param argument="trimnonoverlapping" type="boolean" truevalue="t" falsevalue="f" checked="false" label="Trim all non-overlapping portions, leaving only consensus sequence. By default, only sequence to the right of the overlap (adapter sequence) is trimmed."/> |
129 <param argument="mininsert" type="integer" value="35" label="Minimum insert size to merge reads"/> | 127 <param argument="mininsert" type="integer" value="35" label="Minimum insert size to merge reads"/> |
130 <param argument="minoverlap" type="integer" value="12" label="Minimum number of overlapping bases to allow merging"/> | 128 <param argument="minoverlap" type="integer" value="12" label="Minimum number of overlapping bases to allow merging"/> |
131 | |
132 <param argument="minq" type="integer" value="9" label="Ignore bases with quality below this"/> | 129 <param argument="minq" type="integer" value="9" label="Ignore bases with quality below this"/> |
133 <param argument="maxq" type="integer" value="41" label="Cap output quality scores at this"/> | 130 <param argument="maxq" type="integer" value="41" label="Cap output quality scores at this"/> |
134 | |
135 <param argument="entropy" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Increase the minimum overlap requirement for low-complexity reads"/> | 131 <param argument="entropy" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Increase the minimum overlap requirement for low-complexity reads"/> |
136 <param argument="efilter" type="integer" value="6" label="Ban overlaps with over this many times the expected number of errors." help="Lower is more strict, -1 disables."/> | 132 <param argument="efilter" type="integer" value="6" label="Ban overlaps with over this many times the expected number of errors." help="Lower is more strict, -1 disables."/> |
137 <param argument="pfilter" type="float" value="0.00004" label="Probability filter to disallow improbable overlaps." help="Higher is stricter. 0 will disable the filter; 1 will allow only perfect overlaps."/> | 133 <param argument="pfilter" type="float" value="0.00004" label="Probability filter to disallow improbable overlaps." help="Higher is stricter. 0 will disable the filter; 1 will allow only perfect overlaps."/> |
138 <param argument="kfilter" type="integer" value="41" label="Ban overlaps that create kmers with count below this value" help="Requires good coverage, 0 disables."/> | 134 <param argument="kfilter" type="integer" value="41" label="Ban overlaps that create kmers with count below this value" help="Requires good coverage, 0 disables."/> |
139 <param argument="usequality" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Take quality factors into account" help="If disabled, quality values are completely ignored, both for overlap detection and filtering. May be useful for data with inaccurate quality values."/> | 135 <param argument="usequality" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Take quality factors into account" help="If disabled, quality values are completely ignored, both for overlap detection and filtering. May be useful for data with inaccurate quality values."/> |
140 | |
141 <conditional name="adapters"> | 136 <conditional name="adapters"> |
142 <param name="selector" type="select" label="Provide adapter sequences to improve accuracy?"> | 137 <param name="selector" type="select" label="Provide adapter sequences to improve accuracy?"> |
143 <option value="wout_adapters" selected="true">No</option> | 138 <option value="wout_adapters" selected="true">No</option> |
144 <option value="with_adapters">Yes, use these adapter sequences</option> | 139 <option value="with_adapters">Yes, use these adapter sequences</option> |
145 </param> | 140 </param> |
147 <when value="with_adapters"> | 142 <when value="with_adapters"> |
148 <param argument="adapter1" type="text" value="" label="Left adapter sequence"/> | 143 <param argument="adapter1" type="text" value="" label="Left adapter sequence"/> |
149 <param argument="adapter2" type="text" value="" label="Right adapter sequence"/> | 144 <param argument="adapter2" type="text" value="" label="Right adapter sequence"/> |
150 </when> | 145 </when> |
151 </conditional> | 146 </conditional> |
152 | |
153 <conditional name="merge_mode"> | 147 <conditional name="merge_mode"> |
154 <param name="selector" type="select" label="Evaluate overlaps via..." help="In the ratio mode, overlaps are decided based on the ratio of matching to mismatching bases. Flat mode scores overlaps based on the total number of mismatching bases only."> | 148 <param name="selector" type="select" label="Evaluate overlaps via..." help="In the ratio mode, overlaps are decided based on the ratio of matching to mismatching bases. Flat mode scores overlaps based on the total number of mismatching bases only."> |
155 <option value="Ratio mode" selected="true">Ratio mode</option> | 149 <option value="Ratio mode" selected="true">Ratio mode</option> |
156 <option value="Flat mode">Flat mode</option> | 150 <option value="Flat mode">Flat mode</option> |
157 </param> | 151 </param> |
251 + | 245 + |
252 FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF | 246 FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF |
253 </help> | 247 </help> |
254 <expand macro="citations"/> | 248 <expand macro="citations"/> |
255 </tool> | 249 </tool> |
256 |