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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 784611c9caf2680d41414ca2880b93a69d719701
author | iuc |
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date | Sun, 18 Aug 2024 10:04:02 +0000 |
parents | 70276425d001 |
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<tool name="bcftools @EXECUTABLE@" id="bcftools_plugin_@PLUGIN_ID@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>plugin Extracts fields from structured annotations such as INFO/CSQ</description> <macros> <token name="@EXECUTABLE@">split-vep</token> <token name="@PLUGIN_ID@">split_vep</token> <import>macros.xml</import> </macros> <expand macro="bio_tools" /> <expand macro="requirements" /> <expand macro="version_command" /> <command detect_errors="aggressive"><![CDATA[ @PREPARE_ENV@ @PREPARE_INPUT_FILE@ #set $section = $sec_restrict @PREPARE_TARGETS_FILE@ @PREPARE_REGIONS_FILE@ bcftools plugin @EXECUTABLE@ ## VCF input section #set $section = $sec_restrict @INCLUDE@ @EXCLUDE@ @REGIONS@ @TARGETS@ @OUTPUT_TYPE@ ## Primary Input/Outputs @INPUT_FILE@ ## Plugin options ## ToDo: Parameters not wrapped yet: -A, -f (not for BCF/VCF output!), -l, -S, -x -a '$a' -c '$c' $d $allow_undef_tags #if $p: -p '$p' #end if #if $s: -s '$s' #end if > '$output_file' ]]> </command> <inputs> <expand macro="macro_input" /> <param argument="-a" type="text" value="CSQ" label="Select INFO annotation to parse" help="Usually, annotations are located in the CSQ, ANN or EFF INFO field, though any arbitrary tag may be entered here." /> <param argument="-c" type="text" label="Enter fields to be extracted, listed either as indexes or names" help="The default type of the new annotation is String but can be also set to Integer/Int or Float/Real by adding ':type' to the field name or index. Examples can be found in the tool help." /> <param argument="-d" type="boolean" label="Enable Output per transcript/allele consequences on a new line rather rather than as comma-separated fields on a single line" truevalue="-d" falsevalue="" /> <param argument="-p" type="text" optional="true" label="Enter prefix of INFO annotations to be created after splitting the CSQ string (optional)" /> <param argument="-s" type="text" optional="true" label="Select transcripts to extract by type and/or consequence (optional)" help="Argument has the form TR:CSQ. TR = transcript: worst,primary,all (default: all). CSQ = consequence: any,missense,missense+,etc (default: any). Examples can be found in the tool help." /> <param argument="--allow-undef-tags" type="boolean" truevalue="--allow-undef-tags" falsevalue="" checked="false" label="Allow undefined tags" help='Print "." for undefined tags' /> <section name="sec_restrict" expanded="false" title="Restrict to"> <expand macro="macro_restrict" /> <expand macro="macro_restrict" type="target" label_type="Target" /> <expand macro="macro_include" /> <expand macro="macro_exclude" /> </section> <expand macro="macro_select_output_type" /> </inputs> <outputs> <expand macro="macro_vcf_output" /> </outputs> <tests> <test> <param name="input_file" ftype="vcf" value="plugin2.vcf" /> <param name="a" value="ANN" /> <param name="c" value="IMPACT,gnomAD_AF:Float" /> <!-- <param name="p" value="NW_"/> This parameter trigger core dump error --> <param name="s" value="worst" /> <param name="output_type" value="v" /> <output name="output_file" ftype="vcf"> <assert_contents> <has_line_matching expression="##INFO=<ID=IMPACT,.+" /> <has_line_matching expression="##INFO=<ID=gnomAD_AF,.+" /> <has_line_matching expression=".+IMPACT=MODERATE;gnomAD_AF=0\.08.+" /> <has_line_matching expression=".+IMPACT=LOW;gnomAD_AF=0\.9443.+" /> <has_line_matching expression=".+IMPACT=MODIFIER;gnomAD_AF=\..+" /> </assert_contents> </output> </test> <!-- Test allow undefined tags option --> <test> <param name="input_file" ftype="vcf" value="plugin2.vcf" /> <param name="a" value="ANN" /> <param name="c" value="IMPACT,gnomAD_AF:Float" /> <!-- <param name="p" value="NW_"/> This parameter trigger core dump error --> <param name="s" value="worst" /> <param name="allow_undef_tags" value="true"/> <param name="output_type" value="v" /> <output name="output_file" ftype="vcf"> <assert_contents> <has_line_matching expression="##INFO=<ID=IMPACT,.+" /> <has_line_matching expression="##INFO=<ID=gnomAD_AF,.+" /> <has_line_matching expression=".+IMPACT=MODERATE;gnomAD_AF=0\.08.+" /> <has_line_matching expression=".+IMPACT=LOW;gnomAD_AF=0\.9443.+" /> <has_line_matching expression=".+IMPACT=MODIFIER;gnomAD_AF=\..+" /> </assert_contents> </output> <assert_command> <has_text text="--allow-undef-tags" /> </assert_command> </test> </tests> <help><![CDATA[ ===================================== bcftools @EXECUTABLE@ plugin ===================================== This plugin allows to extract fields from structured annotations such as ``INFO/CSQ`` created by bcftools/csq or VEP. These fields are then added to the VCF as new ``INFO`` fields. Examples:: # Extract Consequence, IMPACT and gene SYMBOL of the most severe consequence into # INFO annotations starting with the prefix "vep". For brevity, the columns can # be given also as 0-based indexes bcftools +split-vep -c Consequence,IMPACT,SYMBOL -s worst -p vep file.vcf.gz bcftools +split-vep -c 1-3 -s worst -p vep file.vcf.gz # Extract gnomAD_AF subfield into a new INFO/gnomAD_AF annotation of Type=Float so that # numeric filtering can be used. bcftools +split-vep -c gnomAD_AF:Float file.vcf.gz -i 'gnomAD_AF<0.001' # Similar to above, but add the annotation only if the consequence severity is missense # or equivalent. bcftools +split-vep -c gnomAD_AF:Float -s :missense file.vcf.gz @REGIONS_HELP@ @TARGETS_HELP@ @EXPRESSIONS_HELP@ ]]></help> <expand macro="citations" /> </tool>