Mercurial > repos > iuc > bcftools_view
comparison macros.xml @ 4:993ed534cc38 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 3edcac645f34d5a94884dedaf95c5774f4afc5c7
author | iuc |
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date | Sat, 11 Mar 2017 18:05:59 -0500 |
parents | cde34352778e |
children | 741a08398a04 |
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3:cde34352778e | 4:993ed534cc38 |
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1 | |
2 <macros> | 1 <macros> |
3 <token name="@VERSION@">1.3</token> | 2 <token name="@VERSION@">1.3.1</token> |
4 <xml name="stdio"> | 3 <xml name="stdio"> |
5 <stdio> | 4 <stdio> |
6 <exit_code range="1:" /> | 5 <exit_code range="1:" /> |
7 <exit_code range=":-1" /> | 6 <exit_code range=":-1" /> |
8 <regex match="Error:" /> | 7 <regex match="Error:" /> |
9 <regex match="Exception:" /> | 8 <regex match="Exception:" /> |
10 </stdio> | 9 </stdio> |
11 </xml> | 10 </xml> |
12 <xml name="requirements"> | 11 <xml name="requirements"> |
13 <requirements> | 12 <requirements> |
14 <requirement type="package" version="1.3">bcftools</requirement> | 13 <requirement type="package" version="1.3.1">bcftools</requirement> |
15 <!-- conda dependency --> | 14 <!-- conda dependency --> |
16 <requirement type="package" version="1.3">htslib</requirement> | 15 <requirement type="package" version="1.3.2">htslib</requirement> |
17 <!-- htslib provides tabix and bgzip | 16 <yield /> |
18 <requirement type="package" version="0.2.6">tabix</requirement> | |
19 --> | |
20 <requirement type="package" version="1.2">samtools</requirement> | |
21 </requirements> | 17 </requirements> |
22 </xml> | 18 </xml> |
23 <xml name="version_command"> | 19 <xml name="version_command"> |
24 <version_command>bcftools 2>&1 | grep 'Version:'</version_command> | 20 <version_command>bcftools 2>&1 | grep 'Version:'</version_command> |
25 </xml> | 21 </xml> |
46 <token name="@PREPARE_INPUT_FILE@"> | 42 <token name="@PREPARE_INPUT_FILE@"> |
47 <![CDATA[ | 43 <![CDATA[ |
48 ## May need to symlink input if there is an associated | 44 ## May need to symlink input if there is an associated |
49 #set $input_vcf = 'input.vcf.gz' | 45 #set $input_vcf = 'input.vcf.gz' |
50 #if $input_file.is_of_type('vcf') | 46 #if $input_file.is_of_type('vcf') |
51 bgzip -c "$input_file" > $input_vcf && | 47 bgzip -c '$input_file' > $input_vcf && |
52 bcftools index $input_vcf && | 48 bcftools index $input_vcf && |
53 #elif $input_file.is_of_type('vcf_bgzip') | 49 #elif $input_file.is_of_type('vcf_bgzip') |
54 ln -s "$input_file" $input_vcf | 50 ln -s '$input_file' $input_vcf |
55 #elif $input_file.is_of_type('bcf') | 51 #elif $input_file.is_of_type('bcf') |
56 #set $input_vcf = 'input.bcf' | 52 #set $input_vcf = 'input.bcf' |
57 ln -s "$input_file" $input_vcf && | 53 ln -s '$input_file' $input_vcf && |
58 #if $input_file.metadata.bcf_index: | 54 #if $input_file.metadata.bcf_index: |
59 ln -s $input_file.metadata.bcf_index ${input_vcf}.csi && | 55 ln -s '${input_file.metadata.bcf_index}' ${input_vcf}.csi && |
60 #else | 56 #else |
61 bcftools index $input_vcf && | 57 bcftools index $input_vcf && |
62 #end if | 58 #end if |
63 #elif $input_file.is_of_type('bcf_bgzip') | 59 #elif $input_file.is_of_type('bcf_bgzip') |
64 ln -s "$input_file" $input_vcf | 60 ln -s '$input_file' $input_vcf |
65 #end if | 61 #end if |
66 ]]> | 62 ]]> |
67 </token> | 63 </token> |
68 <token name="@INPUT_FILE@"> | 64 <token name="@INPUT_FILE@"> |
69 $input_vcf | 65 $input_vcf |
75 <token name="@PREPARE_INPUT_FILES@"> | 71 <token name="@PREPARE_INPUT_FILES@"> |
76 <![CDATA[ | 72 <![CDATA[ |
77 ## May need to symlink input if there is an associated | 73 ## May need to symlink input if there is an associated |
78 #set $input_vcfs = [] | 74 #set $input_vcfs = [] |
79 #set $vcfs_list_file = 'vcfs_list' | 75 #set $vcfs_list_file = 'vcfs_list' |
80 #for (i,input_file) in enumerate($input_files): | 76 #for (i, input_file) in enumerate($input_files): |
81 #set $input_vcf = 'input' + str($i) + '.vcf.gz' | 77 #set $input_vcf = 'input' + str($i) + '.vcf.gz' |
82 #if $input_file.is_of_type('vcf') | 78 #if $input_file.is_of_type('vcf') |
83 bgzip -c "$input_file" > $input_vcf && | 79 bgzip -c '$input_file' > $input_vcf && |
84 bcftools index $input_vcf && | 80 bcftools index $input_vcf && |
85 #elif $input_file.is_of_type('vcf_bgz') | 81 #elif $input_file.is_of_type('vcf_bgz') |
86 ln -s "$input_file" $input_vcf | 82 ln -s '$input_file' $input_vcf |
87 #elif $input_file.is_of_type('bcf') | 83 #elif $input_file.is_of_type('bcf') |
88 #set $input_vcf = 'input' + str($i) + '.bcf.gz' | 84 #set $input_vcf = 'input' + str($i) + '.bcf.gz' |
89 ## bgzip -c "$input_file" > $input_vcf && | 85 ln -s '$input_file' $input_vcf && |
90 ln -s "$input_file" $input_vcf && | |
91 #if $input_file.metadata.bcf_index: | 86 #if $input_file.metadata.bcf_index: |
92 ln -s $input_file.metadata.bcf_index ${input_vcf}.csi && | 87 ln -s '${input_file.metadata.bcf_index}' ${input_vcf}.csi && |
93 #else | 88 #else |
94 bcftools index $input_vcf && | 89 bcftools index $input_vcf && |
95 #end if | 90 #end if |
96 #elif $input_file.is_of_type('bcfvcf_bgz') | 91 #elif $input_file.is_of_type('bcfvcf_bgz') |
97 ln -s "$input_file" $input_vcf && | 92 ln -s '$input_file' $input_vcf && |
98 #end if | 93 #end if |
99 echo '$input_vcf' >> $vcfs_list_file && | 94 echo '$input_vcf' >> $vcfs_list_file && |
100 $input_vcfs.append($input_vcf) | 95 $input_vcfs.append($input_vcf) |
101 #end for | 96 #end for |
102 ]]> | 97 ]]> |
107 <token name="@INPUT_LIST_FILE@"> | 102 <token name="@INPUT_LIST_FILE@"> |
108 $vcfs_list_file | 103 $vcfs_list_file |
109 </token> | 104 </token> |
110 | 105 |
111 <xml name="macro_fasta_ref"> | 106 <xml name="macro_fasta_ref"> |
112 <param name="fasta_ref" type="data" format="data" label="Fasta Ref" optional="True" help="reference sequence in fasta format" /> | 107 <param name="fasta_ref" argument="--fasta-ref" type="data" format="data" label="Reference sequence in FASTA format" optional="True" /> |
113 </xml> | 108 </xml> |
114 <token name="@PREPARE_FASTA_REF@"> | 109 <token name="@PREPARE_FASTA_REF@"> |
115 <![CDATA[ | 110 <![CDATA[ |
116 #set $input_fa_ref = None | 111 #set $input_fa_ref = None |
117 #if 'fasta_ref' in $section and $section.fasta_ref: | 112 #if 'fasta_ref' in $section and $section.fasta_ref: |
118 #set $input_fa_ref = 'ref.fa' | 113 #set $input_fa_ref = 'ref.fa' |
119 ln -s $section.fasta_ref $input_fa_ref && | 114 ln -s '$section.fasta_ref' $input_fa_ref && |
120 samtools faidx $input_fa_ref && | 115 samtools faidx $input_fa_ref && |
121 #end if | 116 #end if |
122 ]]> | 117 ]]> |
123 </token> | 118 </token> |
124 <token name="@FASTA_REF@"> | 119 <token name="@FASTA_REF@"> |
125 #if $input_fa_ref is not None: | 120 #if $input_fa_ref is not None: |
126 --fasta-ref "$input_fa_ref" | 121 --fasta-ref $input_fa_ref |
127 #elif 'fasta_ref' in $section and $section.fasta_ref: | 122 #elif 'fasta_ref' in $section and $section.fasta_ref: |
128 --fasta-ref "${section.fasta_ref}" | 123 --fasta-ref '${section.fasta_ref}' |
129 #end if | 124 #end if |
130 </token> | 125 </token> |
131 | 126 |
132 <xml name="macro_ref_fasta"> | 127 <xml name="macro_ref_fasta"> |
133 <conditional name="reference_source"> | 128 <conditional name="reference_source"> |
149 </conditional> | 144 </conditional> |
150 </xml> | 145 </xml> |
151 | 146 |
152 | 147 |
153 <xml name="macro_AF_file"> | 148 <xml name="macro_AF_file"> |
154 <param name="AF_file" type="data" format="data" label="Af File" optional="True" help="read allele frequencies from file (CHR\tPOS\tREF,ALT\tAF)" /> | 149 <param name="AF_file" argument="--AF-file" type="data" format="tabular" label="Allele frequencies file" optional="True" help="Tab-delimited file containing the columns CHR,POS,REF,ALT,AF" /> |
155 </xml> | 150 </xml> |
156 <!-- This may need to bgzip and tabix the file --> | 151 <!-- This may need to bgzip and tabix the file --> |
157 <token name="@PREPARE_AF_FILE@"> | 152 <token name="@PREPARE_AF_FILE@"> |
158 <![CDATA[ | 153 <![CDATA[ |
159 #if 'AF_file' in $section and $section.AF_file: | 154 #if 'AF_file' in $section and $section.AF_file: |
160 #pass | 155 #pass |
161 #end if | 156 #end if |
162 ]]> | 157 ]]> |
163 </token> | 158 </token> |
164 <token name="@AF_FILE@"> | 159 <token name="@AF_FILE@"> |
165 #if 'AF_file' in $section and $section.AF_file: | 160 #if 'AF_file' in $section and $section.AF_file: |
166 --AF-file "${section.AF_file}" | 161 --AF-file '${section.AF_file}' |
167 #end if | 162 #end if |
168 </token> | 163 </token> |
169 | 164 |
170 <xml name="macro_estimate_AF"> | 165 <xml name="macro_estimate_AF"> |
171 <param name="estimate_AF" type="data" format="data" label="Estimate Af" optional="True" help="calculate AC,AN counts on the fly, using either all samples ("-") or samples listed in <file>" /> | 166 <param name="estimate_AF" argument="--estimate-AF" type="data" format="data" label="Estimate allele frequency" optional="True" help="calculate AC,AN counts on the fly, using either all samples ("-") or samples listed in <file>" /> |
172 </xml> | 167 </xml> |
173 <token name="@ESTIMATE_AF@"> | 168 <token name="@ESTIMATE_AF@"> |
174 #if 'estimate_AF' in $section and $section.estimate_AF: | 169 #if 'estimate_AF' in $section and $section.estimate_AF: |
175 --estimate-AF "${section.estimate_AF}" | 170 --estimate-AF "${section.estimate_AF}" |
176 #end if | 171 #end if |
220 <yield/> | 215 <yield/> |
221 </param> | 216 </param> |
222 </xml> | 217 </xml> |
223 <token name="@COLLAPSE@"> | 218 <token name="@COLLAPSE@"> |
224 #if $section.collapse: | 219 #if $section.collapse: |
225 --collapse "${section.collapse}" | 220 --collapse ${section.collapse} |
226 #end if | 221 #end if |
227 </token> | 222 </token> |
228 | 223 |
229 <xml name="macro_apply_filters"> | 224 <xml name="macro_apply_filters"> |
230 <param name="apply_filters" type="text" value="" label="Apply Filters" optional="true" | 225 <param name="apply_filters" type="text" value="" label="Apply Filters" optional="true" |
232 <validator type="regex" message="FILTER terms separated by commas">^([^ \t\n\r\f\v,]+(,[^ \t\n\r\f\v,]+)*)?$</validator> | 227 <validator type="regex" message="FILTER terms separated by commas">^([^ \t\n\r\f\v,]+(,[^ \t\n\r\f\v,]+)*)?$</validator> |
233 </param> | 228 </param> |
234 </xml> | 229 </xml> |
235 <token name="@APPLY_FILTERS@"> | 230 <token name="@APPLY_FILTERS@"> |
236 #if $section.apply_filters: | 231 #if $section.apply_filters: |
237 --apply-filters "${section.apply_filters}" | 232 --apply-filters '${section.apply_filters}' |
238 #end if | 233 #end if |
239 </token> | 234 </token> |
240 | 235 |
241 <xml name="macro_select_output_type"> | 236 <xml name="macro_select_output_type"> |
242 <param name="output_type" type="select"> | 237 <param name="output_type" type="select"> |
284 </when> | 279 </when> |
285 </conditional> | 280 </conditional> |
286 </xml> | 281 </xml> |
287 <token name="@REGIONS@"> | 282 <token name="@REGIONS@"> |
288 #if $section.regions.regions_src == 'regions' and $section.regions.regions != '': | 283 #if $section.regions.regions_src == 'regions' and $section.regions.regions != '': |
289 --regions "$section.regions.regions" | 284 --regions '$section.regions.regions' |
290 #elif $section.regions.regions_src == 'regions_file' and $section.regions.regions_file: | 285 #elif $section.regions.regions_src == 'regions_file' and $section.regions.regions_file: |
291 --regions-file "$section.regions.regions_file" | 286 --regions-file '$section.regions.regions_file' |
292 #end if | 287 #end if |
293 </token> | 288 </token> |
294 | 289 |
295 <xml name="macro_targets_file"> | 290 <xml name="macro_targets_file"> |
296 <param name="targets_file" type="data" format="tabular" label="Targets File" help="restrict to targets listed in a file" > | 291 <param name="targets_file" type="data" format="tabular" label="Targets File" help="restrict to targets listed in a file" > |
349 <![CDATA[ | 344 <![CDATA[ |
350 #if $targets_path: | 345 #if $targets_path: |
351 --targets-file "${section.targets.invert_targets_file}${targets_path}" | 346 --targets-file "${section.targets.invert_targets_file}${targets_path}" |
352 #else: | 347 #else: |
353 #if $section.targets.targets_src == 'targets' and $section.targets.targets != '': | 348 #if $section.targets.targets_src == 'targets' and $section.targets.targets != '': |
354 --targets "${section.targets.invert_targets_file}${section.targets.targets}" | 349 --targets '${section.targets.invert_targets_file}${section.targets.targets}' |
355 #elif $section.targets.targets_src == 'targets_file' and $section.targets.targets_file: | 350 #elif $section.targets.targets_src == 'targets_file' and $section.targets.targets_file: |
356 --targets-file "${section.targets.invert_targets_file}${section.targets.targets_file}" | 351 --targets-file "${section.targets.invert_targets_file}${section.targets.targets_file}" |
357 #end if | 352 #end if |
358 #end if | 353 #end if |
359 ]]> | 354 ]]> |
373 </xml> | 368 </xml> |
374 <token name="@SAMPLES@"> | 369 <token name="@SAMPLES@"> |
375 #set $samples_defined = False | 370 #set $samples_defined = False |
376 #if str($section.samples) != '': | 371 #if str($section.samples) != '': |
377 #set $samples_defined = True | 372 #set $samples_defined = True |
378 --samples "${section.invert_samples}${section.samples}" | 373 --samples '${section.invert_samples}${section.samples}' |
379 #end if | 374 #end if |
380 #if $section.samples_file: | 375 #if $section.samples_file: |
381 #set $samples_defined = True | 376 #set $samples_defined = True |
382 --samples-file "${section.invert_samples_file}${section.samples_file}" | 377 --samples-file "${section.invert_samples_file}${section.samples_file}" |
383 #end if | 378 #end if |
386 <xml name="macro_sample"> | 381 <xml name="macro_sample"> |
387 <param name="sample" type="text" label="Sample" optional="True" help="apply variants of the given sample" /> | 382 <param name="sample" type="text" label="Sample" optional="True" help="apply variants of the given sample" /> |
388 </xml> | 383 </xml> |
389 <token name="@SAMPLE@"> | 384 <token name="@SAMPLE@"> |
390 #if $section.sample: | 385 #if $section.sample: |
391 --sample "${section.sample}" | 386 --sample '${section.sample}' |
392 #end if | 387 #end if |
393 </token> | 388 </token> |
394 | 389 |
395 | 390 |
396 <xml name="macro_include"> | 391 <xml name="macro_include"> |
423 <validator type="regex" message="COLUMN names separated by commas">^([^,]+(,[^,]+)*)?$</validator> | 418 <validator type="regex" message="COLUMN names separated by commas">^([^,]+(,[^,]+)*)?$</validator> |
424 </param> | 419 </param> |
425 </xml> | 420 </xml> |
426 <token name="@COLUMNS@"> | 421 <token name="@COLUMNS@"> |
427 #if $section.columns != '': | 422 #if $section.columns != '': |
428 --columns "${section.columns}" | 423 --columns '${section.columns}' |
429 #end if | 424 #end if |
430 </token> | 425 </token> |
431 | 426 |
432 <xml name="macro_haploid2diploid"> | 427 <xml name="macro_haploid2diploid"> |
433 <param name="haploid2diploid" type="boolean" truevalue="--haploid2diploid" falsevalue="" label="Haploid2Diploid" help="convert haploid genotypes to diploid homozygotes" /> | 428 <param name="haploid2diploid" type="boolean" truevalue="--haploid2diploid" falsevalue="" label="Haploid2Diploid" help="convert haploid genotypes to diploid homozygotes" /> |
451 and VCF<->BCF conversion. | 446 and VCF<->BCF conversion. |
452 | 447 |
453 This Galaxy tool recommends using the compressed BCF format | 448 This Galaxy tool recommends using the compressed BCF format |
454 as piping is not implemented, and uncompressed data would | 449 as piping is not implemented, and uncompressed data would |
455 use unnecessary amounts of space. | 450 use unnecessary amounts of space. |
456 | 451 ]]></token> |
457 ]]></token> | |
458 <token name="@REGIONS_HELP@"> | 452 <token name="@REGIONS_HELP@"> |
459 <![CDATA[ | 453 <![CDATA[ |
460 Region Selections | 454 Region Selections |
461 ----------------- | 455 ----------------- |
462 | 456 |
473 chromosomes, the VCF will always be processed in ascending | 467 chromosomes, the VCF will always be processed in ascending |
474 genomic coordinate order no matter what order they appear in | 468 genomic coordinate order no matter what order they appear in |
475 FILE. Note that overlapping regions in FILE can result in | 469 FILE. Note that overlapping regions in FILE can result in |
476 duplicated out of order positions in the output. This option | 470 duplicated out of order positions in the output. This option |
477 requires indexed VCF/BCF files. | 471 requires indexed VCF/BCF files. |
478 | 472 ]]></token> |
479 ]]></token> | |
480 <token name="@TARGETS_HELP@"><![CDATA[ | 473 <token name="@TARGETS_HELP@"><![CDATA[ |
481 Targets | 474 Targets |
482 ------- | 475 ------- |
483 | 476 |
484 Similar to regions, but the next position is accessed by streaming the whole | 477 Similar to regions, but the next position is accessed by streaming the whole |
539 | both | abbreviation of "-c indels -c snps" | | 532 | both | abbreviation of "-c indels -c snps" | |
540 +------------+----------------------------------------------------------------+ | 533 +------------+----------------------------------------------------------------+ |
541 | id | only records with identical ID column are compatible. | | 534 | id | only records with identical ID column are compatible. | |
542 | | Supportedby bcftools merge only. | | 535 | | Supportedby bcftools merge only. | |
543 +------------+----------------------------------------------------------------+ | 536 +------------+----------------------------------------------------------------+ |
544 | |
545 </token> | 537 </token> |
546 | 538 |
547 <token name="@EXPRESSIONS_HELP@"> | 539 <token name="@EXPRESSIONS_HELP@"> |
548 <![CDATA[ | 540 <![CDATA[ |
549 Expressions | 541 Expressions |
664 TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2) | 656 TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2) |
665 MIN(DP)>35 && AVG(GQ)>50 | 657 MIN(DP)>35 && AVG(GQ)>50 |
666 ID=@file .. selects lines with ID present in the file | 658 ID=@file .. selects lines with ID present in the file |
667 ID!=@~/file .. skip lines with ID present in the ~/file | 659 ID!=@~/file .. skip lines with ID present in the ~/file |
668 MAF[0]<0.05 .. select rare variants at 5% cutoff | 660 MAF[0]<0.05 .. select rare variants at 5% cutoff |
669 | 661 ]]></token> |
670 ]]></token> | |
671 | |
672 | |
673 | |
674 | |
675 </macros> | 662 </macros> |