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changeset 0:ce2477cd3d95 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
author iuc
date Tue, 14 Nov 2017 05:53:20 -0500
parents
children c30fc94e6611
files extract_bcs.xml macros.xml test-data/converted_bcs.fastq test-data/extracted_bcs.fastq test-data/merged_pcr_dupes.bed test-data/merged_pcr_dupes_clnts.bed test-data/merged_pcr_dupes_spurious.bed test-data/merged_pcr_dupes_spurious_filtered_thresh05.bed test-data/pcr_dupes_randomdict.fastq test-data/pcr_dupes_sorted_2.bed test-data/reads.fastq test-data/readswithtail.fastq test-data/readswithtailremoved.fastq test-data/result_original_head.fastq test-data/twomates.sam test-data/tworeads_aln_ends.bed
diffstat 16 files changed, 1817 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/extract_bcs.xml	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,48 @@
+<tool id="bctools_extract_barcodes" name="Extract barcodes" version="@VERSION@">
+    <description>according to pattern</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <command detect_errors="exit_code"><![CDATA[
+        extract_bcs.py
+        '$reads'
+        #if $barcode_pattern and $barcode_pattern is not None:
+            '$barcode_pattern'
+        #end if
+        --bcs '$extracted_barcodes'
+        > '$reads_cleaned'
+    ]]></command>
+    <inputs>
+        <param name="reads" type="data" format="fastq" label="Barcoded sequences" />
+        <param name="barcode_pattern" type="text" label="Pattern for extracting barcode nucleotides" help="Starting at 5'-end. X positions will be moved to a separate file, N positions will be kept.">
+            <sanitizer invalid_char="N">
+                <valid initial="none">
+                    <add value="X"/>
+                    <add value="N"/>
+                </valid>
+            </sanitizer>
+        </param>
+    </inputs>
+    <outputs>
+        <data name="reads_cleaned" format="fastq"/>
+        <data name="extracted_barcodes" format="fastq"/>
+    </outputs>
+    <tests>
+        <test>
+            <param name="reads" value="reads.fastq"/>
+            <param name="barcode_pattern" value="XXXNNXXX"/>
+            <output name="reads_cleaned" file="result_original_head.fastq"/>
+            <output name="extracted_barcodes" file="extracted_bcs.fastq"/>
+        </test>
+    </tests>
+    <help><![CDATA[
+
+bctools - Extract barcodes according to pattern
+===============================================
+
+Exract barcodes from a FASTQ file according to a user-specified pattern and write them to a separate FASTQ file.
+
+    ]]></help>
+    <expand macro="citations"/>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,13 @@
+<macros>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="@VERSION@">bctools</requirement>
+        </requirements>
+    </xml>
+    <token name="@VERSION@">0.2.1</token>
+    <xml name="citations">
+        <citations>
+            <citation type="doi">10.1016/j.molcel.2013.07.001</citation>
+        </citations>
+    </xml>
+</macros>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/converted_bcs.fastq	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,400 @@
+@UID0
+NYYRRY
++
+#0<FFF
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++
+BBBFFF
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/extracted_bcs.fastq	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,400 @@
+@UID0
+NCTAAT
++
+#0<FFF
+@UID1
+GGTACG
++
+BBBFFF
+@UID2
+GCTAAT
++
+B<BF<B
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++
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++
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++
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++
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++
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/merged_pcr_dupes.bed	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,3 @@
+chr1	10	20	readid_11	10	+
+chr1	10	20	readid_0	5	-
+chrX	20	30	readid_10	7	+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/merged_pcr_dupes_clnts.bed	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,3 @@
+chr1	9	10	readid_11	10	+
+chr1	20	21	readid_0	5	-
+chrX	19	20	readid_10	7	+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/merged_pcr_dupes_spurious.bed	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,12 @@
+chr1	10	20	AAAAA	1	+
+chr1	10	20	AAAAA	2	+
+chr1	10	20	AAAAA	3	+
+chr1	10	20	AAAAA	4	+
+chr1	10	20	AAAAA	5	+
+chr1	10	20	AAAAA	10	+
+chr1	10	20	AAAAA	100	+
+chr1	10	20	AAAAA	3	-
+chr1	10	20	AAAAA	5	-
+chr1	10	20	AAAAA	4	-
+chr1	10	20	AAAAA	5	-
+chrX	20	30	TTTTT	7	+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/merged_pcr_dupes_spurious_filtered_thresh05.bed	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,6 @@
+chr1	10	20	AAAAA	5	-
+chr1	10	20	AAAAA	5	-
+chr1	10	20	AAAAA	4	-
+chr1	10	20	AAAAA	3	-
+chr1	10	20	AAAAA	100	+
+chrX	20	30	TTTTT	7	+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/pcr_dupes_randomdict.fastq	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,88 @@
+@readid_0
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++
+BBBBB
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++
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++
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++
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++
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++
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++
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++
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++
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++
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++
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++
+BBBBB
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++
+BBBBB
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++
+BBBBB
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++
+BBBBB
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++
+BBBBB
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++
+BBBBB
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+BBBBB
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++
+BBBBB
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+AAAAA
++
+BBBBB
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+TTTTT
++
+BBBBB
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++
+BBBBB
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/pcr_dupes_sorted_2.bed	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,22 @@
+chr1	10	20	readid_0	0	-
+chr1	10	20	readid_1	0	-
+chr1	10	20	readid_2	0	+
+chr1	10	20	readid_4	0	+
+chr1	10	20	readid_5	0	+
+chr1	10	20	readid_6	0	-
+chr1	10	20	readid_8	0	+
+chr1	10	20	readid_9	0	-
+chr1	10	20	readid_11	0	+
+chr1	10	20	readid_15	0	+
+chr1	10	20	readid_16	0	+
+chr1	10	20	readid_17	0	-
+chr1	10	20	readid_18	0	+
+chr1	10	20	readid_19	0	+
+chr1	10	20	readid_21	0	+
+chrX	20	30	readid_3	0	+
+chrX	20	30	readid_7	0	+
+chrX	20	30	readid_10	0	+
+chrX	20	30	readid_12	0	+
+chrX	20	30	readid_13	0	+
+chrX	20	30	readid_14	0	+
+chrX	20	30	readid_20	0	+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/reads.fastq	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,400 @@
+@UID0
+NCTAGAATGAAAAATGTATGCTTGTGACCAATAAATATAAAAGGTTAAGATCGGAAGAGCACACGTCTG
++
+#0<FFFFFFFFFFFFIFIIIIIIIIIIIIIIFIIIIIIFIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
+@UID1
+GGTTAACGCCAGTTGGTCCGAGTGTTGTGGGTTATTGTTAACTAAGATCGGAAGAGCACACGTCTGAAC
++
+BBBFFFFFFFFFFIIIIIIIFFFFFIIIIIIFIFIIIIIIIIIFFIFIIIIFFIIFFFFFFFFFFFBFB
+@UID2
+GCTAGAATCCTTTTCCTTTGCCCTGATCTTGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT
++
+B<B<BF<BFBFBFIFFFBFFFFFFFBBB0BBFFFBB<FB707'BFF0FFB'<B<BF#############
+@UID3
+CATTCACGTGGTTGGCGTTTGGTGTGTGGCTGTTTCTGCTCATTGATAGATCGGAAGAGCACACGTCTG
++
+BBBFFFFFFFFFFIIFFFFIIIFFFFFFIFIIFIIFFIFIIIFIIFFIIIIFIIFFFFFFFFFFFFBFF
+@UID4
+NAAAGAATACTGCTTTAATTCTAAATAAAAATTTATTGCCAAGATCGGAAGAGCACACGTCTGAACTCC
++
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++
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+CAATCACGAAGTGTCCTCAGCAAAAGCTGGGTGGTTACCAAGATCGGAAGAGCACACGTCTGAACTCCA
++
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+NCCGAGGTCAATCCGAGTCACGGCACCATGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC
++
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+@UID8
+ACAAGAATAATCTTCTTAGAGTTGGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCA
++
+BBBFFFFFFFFFFIFFIFI<FBFFFFFFIFFFFFFIBFBBFBFFFFFFFFFBBFFBB<7BFFF<<BB<B
+@UID9
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/readswithtail.fastq	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,8 @@
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++
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++
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
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@@ -0,0 +1,8 @@
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++
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++
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
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@@ -0,0 +1,400 @@
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/twomates.sam	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,5 @@
+@HD	VN:1.0	SO:unsorted
+@SQ	SN:chr1	LN:249250621
+@PG	ID:bowtie2	PN:bowtie2	VN:2.2.0	CL:"/usr/local/user/bowtie2/bowtie2-2.2.0/bowtie2-align-s --wrapper basic-0 --very-sensitive --end-to-end --no-mixed --no-discordant --fr --omit-sec-seq --minins=0 --maxins=200 --threads 24 --time -x ../genome/hg19/hg19 --passthrough -1 /tmp/10410.inpipe1 -2 /tmp/10410.inpipe2"
+39V34V1:167:HAWHUADXX:1:1101:19513:10724	99	chr1	45244081	40	41M	=	45244081	-41	GTCCAGTTCTGCTACCGACAGTAAGTGAAGATAAAGTGTGT	FFFIIIFIIIIIIFIIIIIIIFIIIBFFIIIIIIIIFFFFF	AS:i:-6	XN:i:0	XM:i:1	XO:i:0	XG:i:0	NM:i:1	MD:Z:15T25	YS:i:-6	YT:Z:CP
+39V34V1:167:HAWHUADXX:1:1101:19513:10724	147	chr1	45244081	40	41M	=	45244081	-41	GTCCAGTTCTGCTACCGACAGTAAGTGAAGATAAAGTGTGT	IFIIFIIIFIIIIIIIIIIIIIIIIIIIIIIIIFFFFFFFF	AS:i:-6	XN:i:0	XM:i:1	XO:i:0	XG:i:0	NM:i:1	MD:Z:15T25	YS:i:-6	YT:Z:CP
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tworeads_aln_ends.bed	Tue Nov 14 05:53:20 2017 -0500
@@ -0,0 +1,1 @@
+chr1	45244080	45244121	39V34V1:167:HAWHUADXX:1:1101:19513:10724	2	+