Mercurial > repos > iuc > bctools_remove_spurious_events
diff rm_spurious_events.xml @ 0:9ad7bf370022 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
author | iuc |
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date | Tue, 14 Nov 2017 05:54:14 -0500 |
parents | |
children | 84aff696aa97 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/rm_spurious_events.xml Tue Nov 14 05:54:14 2017 -0500 @@ -0,0 +1,48 @@ +<tool id="bctools_remove_spurious_events" name="Remove spurious" version="@VERSION@"> + <description>crosslinking events</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ + rm_spurious_events.py + '$events' + --threshold '$threshold' + --outfile '$events_filtered' + ]]></command> + <inputs> + <param name="events" type="data" format="bed" label="BED6 file containing alignments"/> + <param name="threshold" argument="--threshold" type="float" value="0.1" optional="true" label="Threshold for spurious event removal" help="Remove all crosslinking events that combine less reads than this fraction of the maximum number of reads at this position."/> + </inputs> + <outputs> + <data name="events_filtered" format="bed"/> + </outputs> + <tests> + <test> + <param name="events" value="merged_pcr_dupes_spurious.bed"/> + <param name="threshold" value="0.5"/> + <output name="events_filtered" file="merged_pcr_dupes_spurious_filtered_thresh05.bed"/> + </test> + </tests> + <help><![CDATA[ + +bctools - Remove spurious events originating from errors in unique molecular identifiers (UMIs) +=============================================================================================== + +This tool compares all events sharing the same coordinates. Among each group +of events the maximum number of PCR duplicates is determined. By default, all events that +are supported by less than 10 percent of this maximum count are removed. + +Input +----- + +* BED6 containing crosslinking events with score field set to number of PCR duplicates + +Output +------ + +* BED6 with spurious crosslinking events removed, sorted by fields chrom, start, stop, strand + + ]]></help> + <expand macro="citations"/> +</tool>