Mercurial > repos > iuc > bedtools
view multiCov.xml @ 43:07e8b80f278c draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit db0b91a784ca0c216345bc488d7d488babf1b53f"
author | iuc |
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date | Fri, 01 Apr 2022 19:02:51 +0000 |
parents | 7ab85ac5f64b |
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<tool id="bedtools_multicovtbed" name="bedtools MultiCovBed" version="@TOOL_VERSION@" profile="@PROFILE@"> <description>counts coverage from multiple BAMs at specific intervals</description> <macros> <import>macros.xml</import> </macros> <expand macro="bio_tools" /> <expand macro="requirements" /> <expand macro="stdio" /> <command><![CDATA[ #for $i, $bam in enumerate($bams): ln -s -f '$bam' ${i}.bam && ln -s -f '$bam.metadata.bam_index' ${i}.bam.bai && #end for bedtools multicov -bed '$input' -bams #for $i, $bam in enumerate($bams): '${i}.bam' #end for $strand @OVERLAP@ $reciprocal $split -q $q $duplicate $failed $proper > '$output' ]]></command> <inputs> <param name="input" argument="-bed" type="data" format="@STD_BEDTOOLS_INPUTS@" label="Sorted @STD_BEDTOOLS_INPUT_LABEL@ file" /> <!-- Additional files, if the user needs more --> <param argument="-bams" type="data" format="bam" multiple="true" label="BAM file" /> <expand macro="strand2" /> <expand macro="overlap" /> <expand macro="reciprocal" /> <expand macro="split" /> <param argument="-q" type="integer" value="0" label="Minimum mapping quality (MAPQ) allowed" /> <param name="duplicate" argument="-D" type="boolean" truevalue="-D" falsevalue="" checked="false" label="Include duplicate reads" help="Default counts non-duplicates only" /> <param name="failed" argument="-F" type="boolean" truevalue="-F" falsevalue="" checked="false" label="Include failed-QC reads" help="Default counts pass-QC reads only"/> <param name="proper" argument="-p" type="boolean" truevalue="-p" falsevalue="" checked="false" label="Only count proper pairs" help="Default counts all alignments with MAPQ > -q argument, regardless of the BAM FLAG field" /> </inputs> <outputs> <data name="output" format_source="input" metadata_source="input" /> </outputs> <tests> <test> <param name="input" value="multiCov1.bed" ftype="bed" /> <param name="bams" value="srma_in3.bam,srma_in3.bam" ftype="bam"/> <param name="q" value="1"/> <param name="split" value=""/> <output name="output" file="multiCovBed_result1.bed" ftype="bed" /> </test> </tests> <help><![CDATA[ **What it does** bedtools multicov, reports the count of alignments from multiple position-sorted and indexed BAM files that overlap intervals in a BED file. Specifically, for each BED interval provided, it reports a separate count of overlapping alignments from each BAM file. @REFERENCES@ ]]></help> <expand macro="citations" /> </tool>