Mercurial > repos > iuc > bedtools
view multiCov.xml @ 2:457b09031d57 draft
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author | iuc |
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date | Tue, 19 May 2015 07:05:36 -0400 |
parents | 82aac94b06c3 |
children | 607c0576c6ab |
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<tool id="bedtools_multicovtbed" name="MultiCovBed" version="@WRAPPER_VERSION@.0"> <description></description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements" /> <expand macro="stdio" /> <command> <![CDATA[ #for $i, $bam in enumerate( $bams ): ln -s -f $bam ${i}.bam && ln -s -f $bam.metadata.bam_index ${i}.bam.bai && #end for bedtools multicov -bed $input -bams #for $i, $bam in enumerate( $bams ): ${i}.bam #end for $strand -f $overlap $reciprocal $split -q $q $duplicate $failed $proper > $output ]]> </command> <inputs> <param name="input" format="bed" type="data" label="Sorted BED file" /> <!-- Additional files, if the user needs more --> <param name="bams" format="bam" type="data" multiple="True" label="BAM file" /> <expand macro="strand2" /> <expand macro="overlap" /> <expand macro="reciprocal" /> <expand macro="split" /> <param name="q" type="integer" value="0" label="Minimum mapping quality (MAPQ) allowed" help="(-q)" /> <param name="duplicate" type="boolean" checked="False" truevalue="-D" falsevalue="" label="Include duplicate reads" help="Default counts non-duplicates only. (-D)" /> <param name="failed" type="boolean" checked="false" truevalue="-F" falsevalue="" label="Include failed-QC reads" help="Default counts pass-QC reads only (-F)"/> <param name="proper" type="boolean" checked="false" truevalue="-p" falsevalue="" label="Only count proper pairs" help="Default counts all alignments with MAPQ > -q argument, regardless of the BAM FLAG field. (-p)" /> </inputs> <outputs> <data name="output" metadata_source="input" format_source="input" /> </outputs> <tests> <test> <param name="input" value="multiCov1.bed" ftype="bed" /> <param name="bams" value="srma_in3.bam,srma_in3.bam" ftpye="bam"/> <param name="q" value="1"/> <param name="split" value="False"/> <output name="output" file="multiCovBed_result1.bed" ftype="bed" /> </test> </tests> <help> <![CDATA[ **What it does** bedtools multicov, reports the count of alignments from multiple position-sorted and indexed BAM files that overlap intervals in a BED file. Specifically, for each BED interval provided, it reports a separate count of overlapping alignments from each BAM file. @REFERENCES@ ]]> </help> <expand macro="citations" /> </tool>