annotate bp_genbank2gff3.xml @ 0:f79bcd53b9a3 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bioperl commit 799339e22181d28cb2b145454d353d6025779636
author iuc
date Fri, 09 Oct 2015 09:19:49 -0400
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f79bcd53b9a3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bioperl commit 799339e22181d28cb2b145454d353d6025779636
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1 <tool id="bp_genbank2gff3" name="Genbank to GFF3" version="1.0">
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2 <description>converter</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="stdio" />
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7 <command><![CDATA[
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8 bp_genbank2gff3.pl
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9 $noinfer
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10 #if str($sofile.sofile) != "__none__":
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11 --sofile
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12 #if str($sofile.sofile) == "url":
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13 "${sofile.so_url}"
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14 #else:
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15 live
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16 #end if
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17 #end if
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18 --outdir -
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19 --ethresh $ethresh
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20 $model
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21 --typesource "${typesource}"
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22
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23 $genbank
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24 > $gff3]]></command>
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25 <inputs>
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26 <param label="Genbank file" name="genbank" type="data" format="gb"/>
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27 <param name="noinfer" truevalue="" falsevalue="--noinfer" checked="true" type="boolean" label="Infer exon/mRNA subfeatures"/>
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28 <conditional name="sofile" label="Sequence Ontology">
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29 <param name="sofile" label="Sequence Ontology File" type="select">
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30 <option value="__none__" selected="True">None specified</option>
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31 <option value="live">Latest Sequence Ontology</option>
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32 <option value="url">User Specified</option>
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33 </param>
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34 <when value="__none__" />
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35 <when value="live" />
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36 <when value="url">
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37 <param name="so_url" label="Sequence Ontology URL" type="text"/>
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38 </when>
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39 </conditional>
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40 <param name="ethresh" label="Error threshold for unflattener" type="select">
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41 <option value="0">Strict</option>
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42 <option value="1" selected="True">Medium</option>
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43 <option value="2">Loose</option>
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44 <option value="3">Ignore Errors</option>
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45 </param>
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46 <param name="model" label="Gene Model" type="select">
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47 <option value="--CDS" selected="True">Default GFF gene model</option>
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48 <option value="--noCDS">Alternate gene-RNA-protein-exon model</option>
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49 </param>
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50 <param name="typesource" label="Sequence Ontology type for landmark feature" help="E.g. chromosome, region, contig" value="contig" type="text" />
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51 </inputs>
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52 <outputs>
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53 <data format="gff3" name="gff3" label="${genbank.name} as GFF3"/>
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54 </outputs>
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55 <tests>
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56 <test>
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57 <param name="genbank" value="seq.gb" />
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58 <param name="noinfer" value="True" />
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59 <output name="gff3" file="seq.gb.0.gff" ftype="gff3" lines_diff="6"/>
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60 </test>
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61 <test>
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62 <param name="genbank" value="seq.gb" />
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63 <output name="gff3" file="seq.gb.1.gff" ftype="gff3" lines_diff="4"/>
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64 </test>
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65 </tests>
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66 <help><![CDATA[
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67 **What it does**:
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68
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69 This tool uses Bio::SeqFeature::Tools::Unflattener and
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70 Bio::Tools::GFF to convert GenBank flatfiles to GFF3 with gene
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71 containment hierarchies mapped for optimal display in gbrowse.
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72
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73 The input files are assumed to be gzipped GenBank flatfiles for refseq
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74 contigs. The files may contain multiple GenBank records.
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75
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76 **Designed for RefSeq**
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77
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78 This script is designed for RefSeq genomic sequence entries. It may
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79 work for third party annotations but this has not been tested.
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80 But see below, Uniprot/Swissprot works, EMBL and possibly EMBL/Ensembl
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81 if you don't mind some gene model unflattener errors (dgg).
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82
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83 **G-R-P-E Gene Model**
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84
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85 Don Gilbert worked this over with needs to produce GFF3 suited to
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86 loading to GMOD Chado databases.
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87
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88 This writes GFF with an alternate, but useful Gene model,
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89 instead of the consensus model for GFF3
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90
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91 [ gene > mRNA> (exon,CDS,UTR) ]
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92
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93 This alternate is
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94
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95 gene > mRNA > polypeptide > exon
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96
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97 means the only feature with dna bases is the exon. The others
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98 specify only location ranges on a genome. Exon of course is a child
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99 of mRNA and protein/peptide.
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100
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101 The protein/polypeptide feature is an important one, having all the
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102 annotations of the GenBank CDS feature, protein ID, translation, GO
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103 terms, Dbxrefs to other proteins.
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104
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105 UTRs, introns, CDS-exons are all inferred from the primary exon bases
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106 inside/outside appropriate higher feature ranges. Other special gene
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107 model features remain the same.
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108
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109 **Authors**
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110
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111 Sheldon McKay (mckays@cshl.edu)
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112
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113 Copyright (c) 2004 Cold Spring Harbor Laboratory.
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114
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115 **Author of hacks for GFF2Chado loading**
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116
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117 Don Gilbert (gilbertd@indiana.edu)
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118 ]]></help>
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119 <citations>
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120 <citation type="doi">10.1101/gr.361602</citation>
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121 </citations>
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122 </tool>