Mercurial > repos > iuc > bracken
diff test-data/reproduce_test_data.sh @ 0:b7b1c8bf7ae0 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bracken commit 851f81495c875ac09d936537ffd2b32e6af2c8c5"
author | iuc |
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date | Wed, 16 Oct 2019 16:57:55 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reproduce_test_data.sh Wed Oct 16 16:57:55 2019 -0400 @@ -0,0 +1,29 @@ +#!/bin/bash + +# This script produces a small kraken2 database containing only a ~1kb portion each of a salmonella and ecoli genome +# It requires kraken2, art and entrez-direct (all available on bioconda) +kraken2-build --db test_db --download_taxonomy +mv test_db/taxonomy/nucl_gb.accession2taxid test_db/taxonomy/nucl_gb.accession2taxid_full +grep -e 'NC_003198.1' -e 'NC_011750.1' test_db/taxonomy/nucl_gb.accession2taxid_full > test_db/taxonomy/nucl_gb.accession2taxid +esearch -db nucleotide -query "NC_003198.1" | efetch -format fasta > NC_003198.1.fasta +esearch -db nucleotide -query "NC_011750.1" | efetch -format fasta > NC_011750.1.fasta +head -n 14 NC_003198.1.fasta > NC_003198.1_1kb.fasta +head -n 14 NC_011750.1.fasta > NC_011750.1_1kb.fasta +kraken2-build --db test_db --add-to-library NC_003198.1_1kb.fasta +kraken2-build --db test_db --add-to-library NC_011750.1_1kb.fasta +kraken2-build --db test_db --build + +# Simulate 100bp reads from ~1kb portions of genomes +art_illumina -sam -i NC_011750.1_1kb.fasta -p -m 300 -f 10 -s 10 -l 100 -o NC_011750.1_simulated_R +art_illumina -sam -i NC_003198.1_1kb.fasta -p -m 300 -f 10 -s 10 -l 100 -o NC_003198.1_simulated_R + +# Generate kraken reports +kraken2 --db test_db --report NC_011750.1_simulated_kraken_report.txt --paired NC_011750.1_simulated_R1.fastq NC_011750.1_simulated_R2.fastq +kraken2 --db test_db --report NC_003198.1_simulated_kraken_report.txt --paired NC_003198.1_simulated_R1.fastq NC_003198.1_simulated_R2.fastq + +# Build bracken kmer distribution files using default kmer-len=35 and read-len=100 +bracken-build -d test_db + +# +# est_abundance.py --kmer_distr test_db/database100mers.kmer_distrib --level S -i NC_003198.1_simulated_kraken_report.txt -o NC_003198.1_simulated_bracken_report.txt +# est_abundance.py --kmer_distr test_db/database100mers.kmer_distrib --level S -i NC_011750.1_simulated_kraken_report.txt -o NC_011750.1_simulated_bracken_report.txt