Mercurial > repos > iuc > bwa_mem2
annotate macros.xml @ 3:bfaa0d22c2e4 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 689a4aeaf70a8e77be8589b1dcceb190f9df626a
author | iuc |
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date | Wed, 26 Jul 2023 20:02:13 +0000 |
parents | 88bcfaea2fb6 |
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rev | line source |
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0
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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1 <macros> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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2 <import>read_group_macros.xml</import> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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3 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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4 <token name="@TOOL_VERSION@">2.2.1</token> |
3
bfaa0d22c2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 689a4aeaf70a8e77be8589b1dcceb190f9df626a
iuc
parents:
1
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5 <token name="@VERSION_SUFFIX@">1</token> |
0
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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6 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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7 <xml name="xrefs"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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8 <xrefs> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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9 <xref type="bio.tools">bwa-mem2</xref> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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10 </xrefs> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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11 </xml> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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12 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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13 <token name="@pipefail@"><![CDATA[set -o | grep -q pipefail && set -o pipefail;]]></token> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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14 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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15 <token name="@set_rg_string@"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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16 #set $rg_string = "@RG\\tID:" + str($rg_id) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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17 #set $rg_string += $format_read_group("\\tSM:", $rg_sm) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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18 #set $rg_string += $format_read_group("\\tPL:", $rg_pl) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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19 #set $rg_string += $format_read_group("\\tLB:", $rg_lb) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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20 #set $rg_string += $format_read_group("\\tCN:", $rg_cn) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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21 #set $rg_string += $format_read_group("\\tDS:", $rg_ds) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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22 #set $rg_string += $format_read_group("\\tDT:", $rg_dt) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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23 #set $rg_string += $format_read_group("\\tFO:", $rg_fo) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
changeset
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24 #set $rg_string += $format_read_group("\\tKS:", $rg_ks) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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25 #set $rg_string += $format_read_group("\\tPG:", $rg_pg) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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26 #set $rg_string += $format_read_group("\\tPI:", $rg_pi) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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27 #set $rg_string += $format_read_group("\\tPU:", $rg_pu) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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28 </token> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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29 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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30 <token name="@set_reference_fasta_filename@"><![CDATA[ |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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31 #if str( $reference_source.reference_source_selector ) == "history": |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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32 #set $reference_fasta_filename = "localref.fa" |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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33 ln -s '${reference_source.ref_file}' '${reference_fasta_filename}' && |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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34 bwa-mem2 index |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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35 '${reference_fasta_filename}' && |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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36 #else: |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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37 #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
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38 #end if |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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39 ]]></token> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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40 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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41 <xml name="requirements"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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42 <requirements> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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43 <requirement type="package" version="@TOOL_VERSION@">bwa-mem2</requirement> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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44 <requirement type="package" version="1.13">samtools</requirement> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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45 </requirements> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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46 </xml> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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47 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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48 <xml name="stdio"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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49 <stdio> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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50 <exit_code range="1:" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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51 <exit_code range=":-1" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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52 <regex match="Error:" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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53 <regex match="Exception:" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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54 <regex match="\[bns_restore_core\] Parse error reading" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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55 </stdio> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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56 </xml> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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57 |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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58 <macro name="reference_source_conditional"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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59 <conditional name="reference_source"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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60 <param name="reference_source_selector" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options. See `Indexes` section of help below"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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61 <option value="cached">Use a built-in genome index</option> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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62 <option value="history">Use a genome from history and build index</option> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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63 </param> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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64 <when value="cached"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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65 <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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66 <options from_data_table="bwa_mem2_indexes"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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67 <filter type="sort_by" column="2" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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68 <validator type="no_options" message="No indexes are available" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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69 </options> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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70 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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71 </param> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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72 </when> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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73 <when value="history"> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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74 <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="You can upload a FASTA sequence to the history and use it as reference" /> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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75 </when> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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76 </conditional> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
parents:
diff
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77 </macro> |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
iuc
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78 |
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79 <macro name="dbKeyActionsBwaMem"> |
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80 <expand macro="dbKeyActions"> |
1
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81 <option type="from_data_table" name="bwa_mem2_indexes" column="1" offset="0"> |
0
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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82 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> |
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83 <filter type="param_value" ref="reference_source.ref_file" column="0"/> |
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84 </option> |
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85 </expand> |
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86 </macro> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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87 |
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88 <macro name="dbKeyActions"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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89 <actions> |
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90 <conditional name="reference_source.reference_source_selector"> |
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91 <when value="cached"> |
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92 <action type="metadata" name="dbkey"> |
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93 <yield /> |
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94 </action> |
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95 </when> |
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96 <when value="history"> |
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97 <action type="metadata" name="dbkey"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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98 <option type="from_param" name="reference_source.ref_file" param_attribute="dbkey" /> |
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99 </action> |
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100 </when> |
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101 </conditional> |
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102 </actions> |
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103 </macro> |
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104 |
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105 <token name="@RG@"> |
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106 |
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107 |
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108 ----- |
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109 |
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110 .. class:: warningmark |
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111 |
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112 **Read Groups are Important!** |
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113 |
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114 One of the recommended best practices in NGS analysis is adding read group information to BAM files. You can do this directly in BWA interface using the |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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115 **Specify read group information?** widget. If you are not familiar with read groups you shold know that this is effectively a way to tag reads with an additional ID. |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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116 This allows you to combine BAM files from, for example, multiple BWA runs into a single dataset. This significantly simplifies downstream processing as |
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117 instead of dealing with multiple datasets you only have to handle only one. This is possible because the read group information allows you to identify |
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118 data from different experiments even if they are combined in one file. Many downstream analysis tools such as variant callers (e.g., FreeBayes or Naive Variant Caller |
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119 present in Galaxy) are aware of read groups and will automatically generate calls for each individual sample even if they are combined within a single file. |
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120 |
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121 **Description of read groups fields** |
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122 |
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123 (from GATK FAQ webpage): |
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124 |
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125 .. csv-table:: |
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126 :header-rows: 1 |
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127 |
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128 Tag,Importance,Definition,Meaning |
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129 "ID","Required","Read group identifier. Each @RG line must have a unique ID. The value of ID is used in the RG tags of alignment records. Must be unique among all read groups in header section. Read group IDs may be modified when merging SAM files in order to handle collisions.","Ideally, this should be a globally unique identify across all sequencing data in the world, such as the Illumina flowcell + lane name and number. Will be referenced by each read with the RG:Z field, allowing tools to determine the read group information associated with each read, including the sample from which the read came. Also, a read group is effectively treated as a separate run of the NGS instrument in tools like base quality score recalibration (a GATK component) -- all reads within a read group are assumed to come from the same instrument run and to therefore share the same error model." |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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130 "SM","Sample. Use pool name where a pool is being sequenced.","Required. As important as ID.","The name of the sample sequenced in this read group. GATK tools treat all read groups with the same SM value as containing sequencing data for the same sample. Therefore it's critical that the SM field be correctly specified, especially when using multi-sample tools like the Unified Genotyper (a GATK component)." |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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131 "PL","Platform/technology used to produce the read. Valid values: ILLUMINA, SOLID, LS454, HELICOS and PACBIO.","Important. Not currently used in the GATK, but was in the past, and may return. The only way to known the sequencing technology used to generate the sequencing data","It's a good idea to use this field." |
82217dccdbcf
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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132 "LB","DNA preparation library identify","Essential for MarkDuplicates","MarkDuplicates uses the LB field to determine which read groups might contain molecular duplicates, in case the same DNA library was sequenced on multiple lanes." |
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133 |
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134 |
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135 **Example of Read Group usage** |
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136 |
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137 Suppose we have a trio of samples: MOM, DAD, and KID. Each has two DNA libraries prepared, one with 400 bp inserts and another with 200 bp inserts. Each of these libraries is run on two lanes of an illumina hiseq, requiring 3 x 2 x 2 = 12 lanes of data. When the data come off the sequencer, we would create 12 BAM files, with the following @RG fields in the header:: |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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138 |
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139 Dad's data: |
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140 @RG ID:FLOWCELL1.LANE1 PL:illumina LB:LIB-DAD-1 SM:DAD PI:200 |
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141 @RG ID:FLOWCELL1.LANE2 PL:illumina LB:LIB-DAD-1 SM:DAD PI:200 |
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142 @RG ID:FLOWCELL1.LANE3 PL:illumina LB:LIB-DAD-2 SM:DAD PI:400 |
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143 @RG ID:FLOWCELL1.LANE4 PL:illumina LB:LIB-DAD-2 SM:DAD PI:400 |
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144 |
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145 Mom's data: |
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146 @RG ID:FLOWCELL1.LANE5 PL:illumina LB:LIB-MOM-1 SM:MOM PI:200 |
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147 @RG ID:FLOWCELL1.LANE6 PL:illumina LB:LIB-MOM-1 SM:MOM PI:200 |
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148 @RG ID:FLOWCELL1.LANE7 PL:illumina LB:LIB-MOM-2 SM:MOM PI:400 |
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149 @RG ID:FLOWCELL1.LANE8 PL:illumina LB:LIB-MOM-2 SM:MOM PI:400 |
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150 |
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151 Kid's data: |
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152 @RG ID:FLOWCELL2.LANE1 PL:illumina LB:LIB-KID-1 SM:KID PI:200 |
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153 @RG ID:FLOWCELL2.LANE2 PL:illumina LB:LIB-KID-1 SM:KID PI:200 |
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154 @RG ID:FLOWCELL2.LANE3 PL:illumina LB:LIB-KID-2 SM:KID PI:400 |
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155 @RG ID:FLOWCELL2.LANE4 PL:illumina LB:LIB-KID-2 SM:KID PI:400 |
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156 |
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157 Note the hierarchical relationship between read groups (unique for each lane) to libraries (sequenced on two lanes) and samples (across four lanes, two lanes for each library). |
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158 </token> |
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159 <token name="@info@"> |
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160 ----- |
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161 |
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162 .. class:: infomark |
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163 |
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164 **More info** |
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165 |
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166 To obtain more information about BWA and ask questions use these resources: |
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167 |
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168 1. https://biostar.usegalaxy.org/ |
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169 2. https://www.biostars.org/ |
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170 3. https://github.com/bwa-mem2/bwa-mem2 |
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171 4. http://bio-bwa.sourceforge.net/ |
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172 |
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173 </token> |
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174 |
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175 <xml name="citations"> |
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176 <citations> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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177 <citation type="doi">10.1093/bioinformatics/btp324</citation> |
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178 <citation type="doi">10.1093/bioinformatics/btp698</citation> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa_mem2 commit 7998bbefd9bfd03bc0e92a922297b503832c0419"
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179 <citation type="bibtex">@misc{1303.3997, |
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180 Author = {Heng Li}, |
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181 Title = {Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM}, |
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182 Year = {2013}, |
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183 Eprint = {arXiv:1303.3997}, |
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184 url = {http://arxiv.org/abs/1303.3997}, |
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185 }</citation> |
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186 </citations> |
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187 </xml> |
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188 |
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189 </macros> |