Mercurial > repos > iuc > chira_collapse
diff chira_collapse.xml @ 0:872249f5495a draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
author | iuc |
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date | Sun, 19 Jan 2020 16:32:04 -0500 |
parents | |
children | 00187b27f5e5 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/chira_collapse.xml Sun Jan 19 16:32:04 2020 -0500 @@ -0,0 +1,48 @@ +<tool id="chira_collapse" name="ChiRA collapse" version="@WRAPPER_VERSION@0"> + <description>deduplicate fastq reads</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <command><![CDATA[ + chira_collapse.py + -i '$in' + -u '$umi_len' + -o '$out' + ]]></command> + + <inputs> + <param format="fastq" name="in" type="data" label="Input FASTQ file"/> + <param name="umi_len" type="integer" value="0" + label="Length of the UMI if present at the 5' end of your reads"/> + </inputs> + <outputs> + <data format="fasta" name="out" label="ChiRA collapse FASTQ on ${on_string}"/> + </outputs> + + <tests> + <test expect_num_outputs="1"> + <param name="in" value="reads.fastq" /> + <output name="out" file="reads.fasta" /> + </test> + </tests> + + <help> + +.. class:: infomark + +**What it does** + +This tool deduplicates the reads from the FASTQ file and writes into a fasta each read once with it's read count. + +**Inputs** + +* Quality and adapter trimmed FASTQ file + +**Outputs** + +* FASTA file with unique sequences. The headers of the sequence are in the following format: >sequence_id|UMI|read_count + + </help> + <expand macro="citations" /> +</tool>