Mercurial > repos > iuc > chira_collapse
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit 8064fa653fe73c9432c76783d6c635d86548d538"
author | iuc |
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date | Sun, 19 Dec 2021 15:47:57 +0000 |
parents | 75342b9ef628 |
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<tool id="chira_collapse" name="ChiRA collapse" version="@TOOL_VERSION@1"> <description>deduplicate fastq reads</description> <macros> <import>macros.xml</import> </macros> <expand macro="bio_tools"/> <expand macro="requirements" /> <command><![CDATA[ chira_collapse.py -i #if $in.ext.endswith(".gz") <(gunzip -c '$in') #else '$in' #end if -u '$umi_len' -o '$out' ]]></command> <inputs> <param format="fastq,fastq.gz" name="in" type="data" label="Input FASTQ file"/> <param name="umi_len" type="integer" value="0" label="Length of the UMI if present at the 5' end of your reads"/> </inputs> <outputs> <data format="fasta" name="out" label="ChiRA collapse FASTQ on ${on_string}"/> </outputs> <tests> <test expect_num_outputs="1"> <param name="in" value="reads.fastq.gz" ftype="fastq.gz"/> <output name="out" file="reads.fasta" /> </test> </tests> <help> .. class:: infomark **What it does** This tool deduplicates the reads from the FASTQ file and writes into a fasta each read once with it's read count. **Inputs** * Quality and adapter trimmed FASTQ file **Outputs** * FASTA file with unique sequences. The headers of the sequence are in the following format: >sequence_id|UMI|read_count </help> <expand macro="citations" /> </tool>