Mercurial > repos > iuc > chira_merge
annotate chira_merge.xml @ 13:b5696beaf6d6 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit baafc05b1428cf0ec56a5171c41f2d429a0e0f96"
author | iuc |
---|---|
date | Thu, 13 Aug 2020 02:43:31 -0400 |
parents | 948f4bc1f030 |
children | 6492560fe1b4 |
rev | line source |
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0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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1 <tool id="chira_merge" name="ChiRA merge" version="@WRAPPER_VERSION@0"> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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2 <description>merge aligned positions</description> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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3 <macros> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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4 <import>macros.xml</import> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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5 </macros> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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6 <expand macro="requirements" /> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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7 <command><![CDATA[ |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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8 chira_merge.py |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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9 -b '$alignments' |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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10 #if str($annotation.choice) == "yes": |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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11 -g '$annotation.gtf' |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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12 #end if |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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13 -so '$segment_overlap' |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
diff
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14 -lt '$length_threshold' |
9
948f4bc1f030
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit a69b18084d0a9ade4e29869f8b0e2ce5fc4e0b73"
iuc
parents:
8
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15 -ao '$alignment_overlap' |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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16 #if str($merge.mode) == "blockbuster": |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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17 -bb |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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18 -d '$merge.distance' |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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changeset
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19 -mc '$merge.min_cluster_height' |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
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20 -mb '$merge.min_block_height' |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
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21 -sc '$merge.scale' |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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22 #else: |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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23 -ls '$merge.min_locus_size' |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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24 #end if |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
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25 #if str($reference.ref_type) == "split": |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
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26 -f1 '$reference.ref_fasta1' |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
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27 -f2 '$reference.ref_fasta2' |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
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28 #end if |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
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29 $chimeric_only |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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30 -o ./ |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
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31 ]]></command> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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32 |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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33 <inputs> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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34 <param format="bed" name="alignments" type="data" label="Input BED file of alignments"/> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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35 <conditional name="annotation"> |
1
ce26b5a859ba
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit 6845f3b028a42acbefb07c5f59fd2e558f50a768"
iuc
parents:
0
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36 <param name="choice" type="select" label="Do you have an annotation in GTF format?"> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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37 <option value="yes">Yes</option> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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38 <option value="no">No</option> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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39 </param> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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40 <when value="yes"> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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41 <param format="gtf,gff" name="gtf" type="data" label="Annotations in GTF format"/> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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42 </when> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
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43 <when value="no"> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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44 <!-- Do nothing --> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
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45 </when> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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46 </conditional> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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47 <param name="segment_overlap" type="float" value="0.7" label="Overlap fraction for merging mapped read positions to segments" min="0" max="1" |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
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48 help="Matching read positions with greater than this fraction overlap are merged into a segment"/> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
diff
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49 <param name="length_threshold" type="float" value="0.9" label="Minimum length of the alignments as a fraction of longest" min="0" max="1" |
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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50 help="Consider the alignments of a that read have at least longer than this value times the longest alignment for that read"/> |
9
948f4bc1f030
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit a69b18084d0a9ade4e29869f8b0e2ce5fc4e0b73"
iuc
parents:
8
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51 <param name="alignment_overlap" type="float" value="0.7" label="Overlap fraction for merging alignments/blocks" min="0" max="1" |
948f4bc1f030
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit a69b18084d0a9ade4e29869f8b0e2ce5fc4e0b73"
iuc
parents:
8
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52 help="Minimum fraction of BED entries/blockbuster blocks that must overlap inorder to merge"/> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
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53 <conditional name="merge"> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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54 <param name="mode" type="select" label="Select the mode of merging"> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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55 <option value="overlap">Overlap based</option> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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56 <option value="blockbuster">Gaussian based (blockbuster)</option> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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57 </param> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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58 <when value="overlap"> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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59 <param name="min_locus_size" type="integer" value="1" label="Minimum locus size" |
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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60 help="Minimum number of alignments to be present to output the merged a locus"/> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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61 </when> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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62 <when value="blockbuster"> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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63 <param name="distance" type="integer" value="30" label="Blockbuster parameter distance" |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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64 help="Minimum distance between two clusters"/> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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65 <param name="min_cluster_height" type="integer" value="10" label="Blockbuster parameter minClusterHeight" |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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66 help="minimum height (readno) of a cluster"/> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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67 <param name="min_block_height" type="integer" value="10" label="Blockbuster parameter minBlockHeight" |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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68 help="minimum height (readno) of a block"/> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
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69 <param name="scale" type="float" value="0.1" label="Blockbuster parameter scale" min="0" max="1" |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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70 help="scale stddev for a single read"/> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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71 </when> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
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72 </conditional> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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73 <conditional name="reference"> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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74 <param name="ref_type" type="select" label="Did you use single or split reference for alignment?"> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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75 <option value="single">Single reference</option> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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76 <option value="split">Split reference</option> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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77 </param> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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78 <when value="split"> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
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|
79 <param format="fasta" name="ref_fasta1" type="data" label="Reference FASTA file" |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
80 help="Reference fasta file"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
81 <param format="fasta" name="ref_fasta2" type="data" label="Second reference FASTA file" |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
82 help="Second reference fasta file."/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
83 </when> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
84 <when value="single"> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
85 <!-- no need to provide a fasta file --> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
86 </when> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
87 </conditional> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
88 <param name="chimeric_only" type="boolean" truevalue="-c" falsevalue="" checked="false" /> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
89 </inputs> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
90 <outputs> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
91 <data format="bed" name="segments_bed" from_work_dir="segments.bed" label="ChiRA aligned read segments on ${on_string}"/> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
92 <data format="tabular" name="merged_bed" from_work_dir="merged.bed" label="ChiRA merged alignments on ${on_string}"/> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
93 </outputs> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
94 |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
95 <tests> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
96 <!-- Test: Merge without annotation --> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
97 <test expect_num_outputs="2"> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
98 <param name="alignments" value="alignments.bed"/> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
99 <param name="choice" value="no" /> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
100 <param name="ref_type" value="split"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
101 <param name="ref_fasta1" value="ref1.fasta"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
102 <param name="ref_fasta2" value="ref2.fasta"/> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
103 <output name="segments_bed" > |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
104 <assert_contents> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
diff
changeset
|
105 <has_text_matching expression="ENSMUST00000037472\t672\t687\t2\|1\|4,ENSMUST00000037472,672,687,\+,14S15M25S\t1\t\+" /> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
106 </assert_contents> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
107 </output> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
108 <output name="merged_bed" > |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
109 <assert_contents> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
110 <has_text_matching expression="ENSMUST00000182010\t408\t477\t\+" /> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
111 <has_text_matching expression="mmu-miR-9-5p\t0\t23\t\+" /> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
112 </assert_contents> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
113 </output> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
114 </test> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
115 <!-- Test: Merge with annotation --> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
116 <test expect_num_outputs="2"> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
117 <param name="alignments" value="alignments.bed" /> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
118 <param name="choice" value="yes" /> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
119 <param name="gtf" value="annotation.gtf" /> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
120 <param name="ref_type" value="split"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
121 <param name="ref_fasta1" value="ref1.fasta"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
122 <param name="ref_fasta2" value="ref2.fasta"/> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
123 <output name="segments_bed" > |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
124 <assert_contents> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
125 <has_text_matching expression="2\t31033086\t31033109\t38\|2\|2,ENSMUST00000113555,1676,1699,\+,32S23M\t1\t-" /> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
126 <has_text_matching expression="7\t83738820\t83738843\t38\|2\|1,mmu-miR-9-5p,0,23,\+,5S23M27S\t1\t\+" /> |
0
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
127 </assert_contents> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
128 </output> |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
129 <output name="merged_bed" > |
5db894671434
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
iuc
parents:
diff
changeset
|
130 <assert_contents> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
131 <has_text_matching expression="2\t31033086\t31033109\t-" /> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
132 <has_text_matching expression="7\t83738820\t83738843\t\+" /> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
133 </assert_contents> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
134 </output> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
135 </test> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
136 <!-- Test: Merge using blockbuster --> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
137 <test expect_num_outputs="2"> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
138 <param name="alignments" value="alignments.bed" /> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
139 <param name="choice" value="yes" /> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
140 <param name="gtf" value="annotation.gtf" /> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
141 <param name="mode" value="blockbuster" /> |
8
c836a05bd6a3
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit d8a9a5bb648d24f3917951cd7862f449e4dd0688"
iuc
parents:
7
diff
changeset
|
142 <param name="min_block_height" value="1" /> |
9
948f4bc1f030
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit a69b18084d0a9ade4e29869f8b0e2ce5fc4e0b73"
iuc
parents:
8
diff
changeset
|
143 <param name="min_cluster_height" value="0" /> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
144 <param name="ref_type" value="split"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
145 <param name="ref_fasta1" value="ref1.fasta"/> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
146 <param name="ref_fasta2" value="ref2.fasta"/> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
147 <output name="segments_bed" > |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
148 <assert_contents> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
149 <has_text_matching expression="2\t31033086\t31033109\t38\|2\|2,ENSMUST00000113555,1676,1699,\+,32S23M\t1\t-" /> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
150 <has_text_matching expression="7\t83738820\t83738843\t38\|2\|1,mmu-miR-9-5p,0,23,\+,5S23M27S\t1\t\+" /> |
4
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
151 </assert_contents> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
152 </output> |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
153 <output name="merged_bed" > |
0170de5072d4
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit aafb8e5de9023eeddd7f4683909f418ef5aae086"
iuc
parents:
1
diff
changeset
|
154 <assert_contents> |
7
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
155 <has_text_matching expression="7\t83738820\t83738843\t\+" /> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
156 </assert_contents> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
157 </output> |
35f433d0bd7f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit ac10509d952583cefd7bf63910cd6976a0124440"
iuc
parents:
4
diff
changeset
|
158 </test> |
35f433d0bd7f
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159 <!-- Test: Chimeric only without annotation --> |
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160 <test expect_num_outputs="2"> |
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161 <param name="alignments" value="alignments.bed"/> |
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162 <param name="choice" value="no" /> |
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163 <param name="ref_type" value="split"/> |
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164 <param name="ref_fasta1" value="ref1.fasta"/> |
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165 <param name="ref_fasta2" value="ref2.fasta"/> |
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166 <param name="chimeric_only" value="true"/> |
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167 <output name="segments_bed" > |
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168 <assert_contents> |
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169 <has_text_matching expression="mmu-miR-9-5p\t0\t23\t38\|2\|1,mmu-miR-9-5p,0,23,\+,5S23M27S\t1\t\+" /> |
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170 <has_text_matching expression="ENSMUST00000113555\t1676\t1699\t38\|2\|2,ENSMUST00000113555,1676,1699,\+,32S23M\t1\t\+" /> |
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171 </assert_contents> |
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172 </output> |
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173 <output name="merged_bed" > |
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174 <assert_contents> |
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175 <has_text_matching expression="ENSMUST00000113555\t1676\t1699\t\+" /> |
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176 <has_text_matching expression="mmu-miR-9-5p\t0\t23\t\+" /> |
0
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177 </assert_contents> |
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178 </output> |
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179 </test> |
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180 </tests> |
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181 |
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182 <help> |
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183 |
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184 .. class:: infomark |
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185 |
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186 **What it does** |
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187 |
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188 This tool merges the overlapping aligned positions to define the read concentrated loci. If an annotation GTF file produced, the transcriptomic alignment positions are first converted to their corresponding genomic positions. |
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189 |
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190 **Inputs** |
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191 |
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192 * Alignments in BED format |
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193 * An annotation GTF file contaning reference genomic positions. |
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194 |
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195 **Output** |
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196 |
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197 * BED file containing the alignments with reads categorized into segments depending on which part of the read is aligned. |
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198 * Tabular file containing merged alignments. 4th column contains all the alignments merged into that location. |
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199 |
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200 </help> |
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201 <expand macro="citations" /> |
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202 </tool> |