Mercurial > repos > iuc > concoct_coverage_table
view concoct_coverage_table.xml @ 4:0442e7102125 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/concoct commit 6bcf71ac8fae2a84117069dc00b3c4cac6d20fa5
author | iuc |
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date | Sat, 26 Aug 2023 02:42:05 +0000 |
parents | 5ded3318cf8a |
children | fd31cd168efc |
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<tool id="concoct_coverage_table" name="CONCOCT: Generate the input coverage table" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>for CONCOCT</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <command detect_errors="exit_code"><![CDATA[ #import re mkdir 'mapping' && #if $mode.type == 'individual' #set $identifier = re.sub('[^\s\w\-\\.]', '_', str($mode.bamfile)) ln -s '$mode.bamfile' 'mapping/${identifier}.sorted.bam' && samtools index 'mapping/${identifier}.sorted.bam' 'mapping/${identifier}.bam.bai' && mv 'mapping/${identifier}.sorted.bam' 'mapping/${identifier}.bam' && #else #for $e in $mode.bamfiles #set $identifier = re.sub('[^\s\w\-\\.]', '_', str($e.element_identifier)) ln -s '$e' 'mapping/${identifier}.sorted.bam' && samtools index 'mapping/${identifier}.sorted.bam' 'mapping/${identifier}.bam.bai' && mv 'mapping/${identifier}.sorted.bam' 'mapping/${identifier}.bam' && #end for #end if concoct_coverage_table.py '$bedfile' mapping/*.bam > '$output' ]]></command> <inputs> <param name="bedfile" type="data" format="bed" label="Contigs BEDFile" help="The file should have four columns representing: 'Contig ID, Start Position, End Position and SubContig ID' respectively. The Subcontig ID must contain the pattern 'concoct_part_[0-9]*' while the contigs which are not cutup cannot contain this pattern. This file can be generated by the CONCOCT: Cut up contigs tool."/> <conditional name="mode"> <param name="type" type="select" label="Type of assembly used to generate the contigs" help="Information used to process the BAM files"> <option value="individual">Individual assembly: 1 run per BAM file</option> <option value="co">Co-assembly: all BAM files processed together</option> </param> <when value="individual"> <param name="bamfile" type="data" format="bam" label="Sorted BAM file" help="BAM file with mappings to the original contigs, after sorting"/> </when> <when value="co"> <param name="bamfiles" type="data" format="bam" label="Sorted BAM files" multiple="true" help="BAM files with mappings to the original contigs, after sorting"/> </when> </conditional> </inputs> <outputs> <data name="output" format="tabular"/> </outputs> <tests> <test expect_num_outputs="1"> <param name="bedfile" value="two_contigs-cut2.bed" ftype="bed"/> <conditional name="mode"> <param name="type" value="individual"/> <param name="bamfile" value="ten_reads_two_contigs-s.bam" ftype="bam"/> </conditional> <output name="output" ftype="tabular"> <assert_contents> <has_text text="contig-21000001.concoct_part_0"/> <has_n_lines n="11"/> </assert_contents> </output> </test> <test expect_num_outputs="1"> <param name="bedfile" value="two_contigs-cut2.bed" ftype="bed"/> <conditional name="mode"> <param name="type" value="co"/> <param name="bamfiles" value="ten_reads_two_contigs-s.bam,ten_reads_two_contigs-s_2.bam" ftype="bam"/> </conditional> <output name="output" ftype="tabular"> <assert_contents> <has_text text="cov_mean_sample_ten_reads_two_contigs-s.bam"/> <has_text text="cov_mean_sample_ten_reads_two_contigs-s_2.bam"/> <has_text text="contig-21000001.concoct_part_0"/> <has_n_lines n="11"/> </assert_contents> </output> </test> </tests> <help><![CDATA[ **What it does** Accepts an assembled (and possibly cut by the Cut fasta contigs tool) fasta contigs file and a tabular coverage histogram file (produced by the bedtools Genomve Coverage tool) and outputs a tabular coverage file for use as the input to the CONCOCT metagenome binning tool. @HELP_OVERVIEW@ ]]></help> <expand macro="citations"/> </tool>