Mercurial > repos > iuc > crosscontamination_barcode_filter
annotate crosscontamination_barcode_filter.xml @ 2:5ade5cf200da draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
| author | iuc |
|---|---|
| date | Wed, 12 Jun 2019 04:58:19 -0400 |
| parents | 253c9448f524 |
| children |
| rev | line source |
|---|---|
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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1 <tool id="crosscontamination_barcode_filter" name="Cross-contamination Barcode Filter" version="@VERSION@"> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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2 <description>for use in plate-based barcoded analyses</description> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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3 <macros> |
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2
5ade5cf200da
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
iuc
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4 <token name="@VERSION@">0.3</token> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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5 <macro name="sanitize_batch"> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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6 <sanitizer invalid_char=""> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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7 <valid initial="string.digits"> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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8 <add value=","/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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9 </valid> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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10 </sanitizer> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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11 </macro> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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12 <macro name="sanitize_regex"> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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13 <sanitizer invalid_char=""> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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14 <valid initial="string.letters,string.digits"> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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15 <add value="!"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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16 <add value="="/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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17 <add value="-"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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18 <add value="."/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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19 <add value="*"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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20 <add value="?"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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21 <add value="+"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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22 <add value="\\"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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23 <add value="_"/> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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24 <add value="["/> <!-- left square bracket, e.g subselecting from vec[1] --> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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25 <add value="]"/> <!-- right square bracket --> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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26 <add value="("/> <!-- left parenthesis --> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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27 <add value=")"/> <!-- right parenthesis --> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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28 </valid> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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changeset
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29 </sanitizer> |
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1
253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
parents:
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30 </macro> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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31 </macros> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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32 <requirements> |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
parents:
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33 <requirement type="package" version="3.1.1">r-ggplot2</requirement> |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
parents:
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34 <requirement type="package" version="1.12.2">r-data.table </requirement> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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35 </requirements> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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36 <version_command><![CDATA[ |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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37 Rscript '$__tool_directory__/scripts/crosscontamination_filter.R' | head -1 | cut -d' ' -f 2 |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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38 ]]></version_command> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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39 <command detect_errors="exit_code"><![CDATA[ |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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40 Rscript '$__tool_directory__/scripts/crosscontamination_filter.R' '$crossconf' |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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41 ]]></command> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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42 <configfiles> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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43 <configfile name="crossconf"><![CDATA[ |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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44 script.dir = '$__tool_directory__/scripts' |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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45 input_matrix <- read.table( |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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46 '$input_table', |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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47 stringsAsFactors = F, |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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48 na.strings=c("NA", "-", "?", "."), |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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49 header=TRUE, |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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50 row.names=1 |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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51 ) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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52 input_matrix[is.na(input_matrix)] <- 0 |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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53 #if str($inbuilt_spec.select_use) == "mpi_sagar": |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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54 spec = list( |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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55 barcodes = '$input_barcodes', |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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56 format = list( |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
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57 "1-96" = c(1,3), |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
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58 "97-192" = c(2,4) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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59 ), |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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60 plates = list( |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
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61 "1" = c(1,2), |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
iuc
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62 "2" = c(3,4) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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63 ) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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64 ) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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65 #elif str($inbuilt_spec.select_use) == "custom": |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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66 spec = list( |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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67 barcodes = '$input_barcodes', |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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68 format = list( |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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69 #for $i, $s in enumerate($inbuilt_spec.barcode_format) |
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253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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70 "${s.range_start}-${s.range_end}" = c( ${s.batches} ) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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71 #if $i < len(list($inbuilt_spec.barcode_format)) - 1 |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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72 , |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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73 #end if |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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74 #end for |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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75 ), |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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76 plates = list( |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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77 #for $i, $s in enumerate($inbuilt_spec.plate_format) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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78 "${s.plate}" = c( ${s.batches} ) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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79 #if $i < len(list($inbuilt_spec.plate_format)) - 1 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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80 , |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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81 #end if |
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82 #end for |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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83 ) |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
parents:
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84 ) |
|
582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
iuc
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85 #end if |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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86 regex.extract = '$advanced.regex_extract' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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87 regex.display = '$advanced.regex_display' |
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2
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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88 sort.cells = as.logical('$advanced.sort_sizes') |
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0
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89 out.pdf = '$out_plots' |
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90 out.table = '$out_table' |
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91 ]]> |
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92 </configfile> |
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93 </configfiles> |
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94 <inputs> |
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95 <param name="input_table" type="data" format="tsv,tabular" label="Input Matrix" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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96 <param name="input_barcodes" type="data" format="tsv,tabular,txt" label="Complete Barcodes" /> |
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582b7bd4ae4c
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97 <conditional name="inbuilt_spec" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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98 <param name="select_use" type="select" label="Plate Protocol" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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99 <option value="mpi_sagar">CelSeq2 Plate Protocol (Sagar)</option> |
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100 <option value="custom">Custom</option> |
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101 </param> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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102 <when value="mpi_sagar" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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103 <when value="custom"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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104 <repeat name="barcode_format" title="Barcode Format" help="e.g. Batches 1 and 4 use barcodes 1-100 in the Barcodes file, and Batches 2 and 3 use barcodes 101-200 in the Barcodes file; specify '1' and '100' as Range values, and '1,4' as Batch values, and in the next format specify '101' and '200' as Range values and '2,3' as Batch values" > |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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105 <param name="range_start" type="integer" min="1" value="1" label="Barcode Range: Start" /> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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106 <param name="range_end" type="integer" min="2" value="100" label="Barcode Range: End" /> |
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582b7bd4ae4c
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107 <param name="batches" type="text" value="1,4" label="Batches utilizing this Range" > |
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108 <expand macro="sanitize_batch" /> |
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109 </param> |
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110 </repeat> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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111 <repeat name="plate_format" title="Plate Format" help="e.g. Plate 1 encompasses Batches 1-4, and Plate 2 encompasses Batches 5-8; specify '1' as a Plate value, and '1,2,3,4' as Batch values, and in the next format specify '2' as a Plate value and '5,6,7,8' as Batch values"> |
|
582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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112 <param name="plate" type="integer" min="1" value="1" label="Plate Number" /> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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113 <param name="batches" type="text" value="1,2,3,4" label="Batches within this Plate Number" > |
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582b7bd4ae4c
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114 <expand macro="sanitize_batch" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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115 </param> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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116 </repeat> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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117 </when> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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118 </conditional> |
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2
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119 <section name="advanced" expanded="false" title="Advanced Parameters" > |
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1
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120 <param name="regex_extract" type="text" value=".*P(\\d)_B(\\d)_([ACTG]+)" label="RegEx to extract Plate, Batch, and Barcodes from headers" > |
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0
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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121 <expand macro="sanitize_regex" /> |
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122 </param> |
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123 <param name="regex_display" type="text" value="P\\1_B\\2_\\3" label="RegEx to replace Plate, Batch, and Barcodes from headers" > |
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582b7bd4ae4c
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124 <expand macro="sanitize_regex" /> |
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125 </param> |
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2
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126 <param name="sort_sizes" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="false" label="Sort Cells by Library Size" /> |
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0
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127 </section> |
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128 </inputs> |
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129 <outputs> |
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130 <data name="out_plots" format="pdf" label="${tool.name} on ${on_string}: Plots" /> |
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131 <data name="out_table" format="tabular" label="${tool.name} on ${on_string}: Filtered Table" /> |
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132 </outputs> |
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133 <tests> |
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134 <test><!-- Inbuilt MPI --> |
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1
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135 <param name="input_table" value="test.matrix" /> |
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0
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136 <param name="input_barcodes" value="celseq_barcodes.192.raw" /> |
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582b7bd4ae4c
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137 <conditional name="inbuilt_spec" > |
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138 <param name="select_use" value="mpi_sagar" /> |
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582b7bd4ae4c
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139 </conditional> |
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1
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140 <output name="out_plots" value="out.pdf" compare="sim_size" /> |
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141 <output name="out_table" value="out.table" /> |
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0
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142 </test> |
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143 <test><!-- Plate and Lane test --> |
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1
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144 <param name="input_table" value="test.matrix" /> |
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0
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145 <param name="input_barcodes" value="celseq_barcodes.192.raw" /> |
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582b7bd4ae4c
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146 <conditional name="inbuilt_spec" > |
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147 <param name="select_use" value="custom" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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148 <repeat name="barcode_format" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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149 <param name="range_start" value="1"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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150 <param name="range_end" value="96" /> |
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1
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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151 <param name="batches" value="1,3" /> |
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0
582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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152 </repeat> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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153 <repeat name="barcode_format" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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154 <param name="range_start" value="97"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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155 <param name="range_end" value="192" /> |
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1
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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156 <param name="batches" value="2,4" /> |
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0
582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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157 </repeat> |
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582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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158 <repeat name="plate_format" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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159 <param name="plate" value="1" /> |
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1
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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160 <param name="batches" value="1,2" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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161 </repeat> |
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162 <repeat name="plate_format" > |
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163 <param name="plate" value="2" /> |
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1
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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164 <param name="batches" value="3,4" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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165 </repeat> |
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166 </conditional> |
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1
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167 <output name="out_plots" value="out.pdf" compare="sim_size" /> |
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168 <output name="out_table" value="out.table" /> |
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169 </test> |
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2
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170 <test><!-- 12 Batch test --> |
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171 <param name="input_table" value="b12.matrix" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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172 <param name="input_barcodes" value="celseq_barcodes.192.raw" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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173 <conditional name="inbuilt_spec" > |
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174 <param name="select_use" value="custom" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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175 <repeat name="barcode_format" > |
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176 <param name="range_start" value="1"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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177 <param name="range_end" value="96" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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178 <param name="batches" value="1,3,5,7,9,11" /> |
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179 </repeat> |
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180 <repeat name="barcode_format" > |
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181 <param name="range_start" value="97"/> |
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182 <param name="range_end" value="192" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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183 <param name="batches" value="2,4,6,8,10,12" /> |
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184 </repeat> |
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185 <repeat name="plate_format" > |
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186 <param name="plate" value="1" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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187 <param name="batches" value="1,2,3,4" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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188 </repeat> |
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189 <repeat name="plate_format" > |
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190 <param name="plate" value="2" /> |
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191 <param name="batches" value="5,6,7,8" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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192 </repeat> |
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193 <repeat name="plate_format" > |
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194 <param name="plate" value="3" /> |
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195 <param name="batches" value="9,10,11,12" /> |
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196 </repeat> |
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197 </conditional> |
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198 <output name="out_plots" value="b12.pdf" compare="sim_size" /> |
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199 <output name="out_table" value="b12.table" /> |
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200 </test> |
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201 </tests> |
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202 <help><![CDATA[ |
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203 Cross-contamination Filter Plot |
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204 ################################### |
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205 |
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206 For a set of barcodes and an experimental setup that uses a subset of these barcodes for each batch, this tool compares each batch against the full range of barcodes in order to determine whether any cross-contamination between batches has occured. |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 82a0fd493f5866b3ef65019709ae5c865998f802
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207 *Note -- Do not reuse batch numbering across plates!* |
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208 |
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209 If a significant number of transcripts are shown in a batch for cell barcodes that were not designed for that batch, then this tool will show that. In the below plot, we can see that there is no significant cross-contamination taking place (pre-filter), and so we can filter out the false barcodes (post-filter). |
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210 |
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211 .. image:: $PATH_TO_IMAGES/crosscontam_pretopost.png |
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212 :scale: 50 % |
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213 |
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214 |
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215 Example |
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216 ~~~~~~~~ |
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217 |
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218 Consider the following experimental setup, with a list of 100 possible barcodes, used over 3 sequencing plates, with each plate containing 4 unique batches, and each plate using a specific subset of the 100 barcodes. |
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219 |
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1
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220 :: |
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0
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221 |
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222 Barcodes |
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1
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223 |
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0
582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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224 1 - 10 | AAA AAC AAT AAG ACA AGA ATA CAC GAG TAT |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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225 11 - 20 | CCC CCA CCT CCG CTC CGC TCT GCG TCT CGT |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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226 . |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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227 . |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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228 91 -100 | TTT TAT TCT TGT TTA TTC TTG TCC TGG TAA |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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229 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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230 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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231 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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232 Plate 1 +-------+-------+-------+-------+ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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233 | B1 | B2 | B3 | B4 | |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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234 +-------+-------+-------+-------+ |
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1
253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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235 1-50 51-100 51-100 1-50 |
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0
582b7bd4ae4c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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236 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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237 Plate 2 +-------+-------+-------+-------+ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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238 | B5 | B6 | B7 | B8 | |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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239 +-------+-------+-------+-------+ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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240 1-40 41-80 1-40 41-80 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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241 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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242 Plate 3 +-------+-------+-------+-------+ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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243 | B9 | B10 | B11 | B12 | |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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244 +-------+-------+-------+-------+ |
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1
253c9448f524
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit f967afe562781e5c8ed4e24e9d1e0bc3ebb29401
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245 1-40 41-80 1-40 41-80 |
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0
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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246 |
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247 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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248 **** |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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249 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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250 The above plate and barcoding setup can be more textually represented by specifying barcode ranges and plate numbers, with each denoting which batch numbers they describe as outlined below: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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251 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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252 :: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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253 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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254 *Barcodes → Batches* |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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255 1- 50: B1, B4 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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256 51-100: B2, B3 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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257 1- 40: B5, B7, B9 , B11 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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258 41- 80: B6, B8, B10, B12 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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259 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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260 *Plates → Batches* |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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261 1: B1, B2 , B3 , B4 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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262 2: B5, B6 , B7 , B8 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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263 3: B9, B10, B11, B12 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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264 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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265 ]]></help> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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266 <citations> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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267 <citation type="doi">10.1007/978-1-4939-7768-0_15</citation> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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268 </citations> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crosscontamination_barcode_filter commit 6f73edc667e61fabdab8b24a7ff40942588fee5b
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269 </tool> |