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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit 3dd3145db6ed58efc3bf5f71e96515173967fc72
author iuc
date Sat, 07 Dec 2024 08:46:39 +0000
parents 65db807336bc
children
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18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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1 <?xml version="1.0"?>
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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2 <macros>
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3 <xml name="requirements">
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4 <requirements>
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5 <requirement type="package" version="@DADA2_VERSION@">bioconductor-dada2</requirement>
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6 <yield/>
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7 </requirements>
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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8 </xml>
6
76d3d2b10738 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit ea6c9c638e742c097b0ef294161eeea447c09e06
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9 <xml name="bio_tools">
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10 <xrefs>
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11 <xref type="bio.tools">dada2</xref>
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12 <xref type="bioconductor">dada2</xref>
76d3d2b10738 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit ea6c9c638e742c097b0ef294161eeea447c09e06
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13 </xrefs>
76d3d2b10738 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit ea6c9c638e742c097b0ef294161eeea447c09e06
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14 </xml>
9
65db807336bc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit 9bbc0898b9bbe73c7fc60ac162d80d749a7f97c1
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15 <token name="@DADA2_VERSION@">1.30.0</token>
65db807336bc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit 9bbc0898b9bbe73c7fc60ac162d80d749a7f97c1
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16 <token name="@WRAPPER_VERSION@">0</token>
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17
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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18 <xml name="version_command">
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19 <version_command><![CDATA[
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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20 echo $(R --version | grep version | grep -v GNU)", dada2 version" $(R --vanilla --slave -e "library(dada2); cat(sessionInfo()\$otherPkgs\$dada2\$Version)" 2> /dev/null | grep -v -i "WARNING: ")
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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21 ]]></version_command>
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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22 </xml>
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23
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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24 <xml name="stdio">
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25 <stdio>
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26 <regex match="Error: cannot allocate" source="stderr" level="fatal_oom" description="Out of memory error occurred" />
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27 <regex match="'Calloc' could not allocate memory" source="stderr" level="fatal_oom" description="Out of memory error occurred" />
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28 </stdio>
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29 </xml>
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30
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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31 <xml name="citations">
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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32 <citations>
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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33 <citation type="doi">10.1038/nmeth.3869</citation>
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34 <yield/>
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35 </citations>
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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36 </xml>
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37
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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38 <token name="@DADA_UNIQUES@">dada2_dada,dada2_mergepairs</token>
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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39
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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40 <!-- function to read dada2 data types
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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41 - dada, and mergepairs are simply read as RDS
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42 - sequence_table is a named integer matrix (rows=samples, columns=ASVs)
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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43 - uniques is a named integer vector (columns=ASVs, only one rows)-->
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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44 <token name="@READ_FOO@"><![CDATA[
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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45 read.uniques <- function ( fname ) {
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46 x <- read.table(fname, header=F, sep="\t")
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47 n <-x[,2]
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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48 names(n)<-x[,1]
7
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49 return(n)
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50 }
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51 #def read_data($dataset)
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52 #if $dataset.is_of_type('dada2_sequencetable')
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53 t(read.table('$dataset', header=T, sep="\t", row.names=1, check.names=FALSE))
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54 #else if $dataset.is_of_type('dada2_uniques')
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55 read.uniques('$dataset')
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56 #else if $dataset.is_of_type('tabular')
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57 read.table('$dataset', header=T, sep="\t", row.names=1, check.names=FALSE)
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58 #else
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59 readRDS('$dataset')
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60 #end if
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61 #end def
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62 ]]></token>
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63 <!-- function to write dada2 data types (the content or the R variable 'out' is written)
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64 - dada, and mergepairs are written as RDS
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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65 - sequence_table is a named integer matrix (rows=samples, columns=ASVs)
18517edb4733 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f8b6b6e72914ad6bcca8423dfa03f59bde80992e"
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66 - uniques is a named integer vector (columns=ASVs, only one rows)-->
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67 <token name="@WRITE_FOO@"><![CDATA[
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68 write.data <- function( data, fname, type ){
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69 if( type == 'dada2_uniques'){
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70 write.table(data, file = fname, quote = F, sep = "\t", row.names = T, col.names = F)
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71 }else if( type== 'dada2_sequencetable'){
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72 write.table(t(data), file=fname, quote=F, sep="\t", row.names = T, col.names = NA)
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73 }else{
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74 saveRDS(data, file=fname)
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75 }
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76 }
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77 ]]></token>
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78
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79 <xml name="fastq_input" token_multiple="" token_collection_type="" token_argument_fwd="" token_argument_rev="">
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80 <conditional name="paired_cond">
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81 <param name="paired_select" type="select" label="Paired reads">
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82 <option value="paired">paired - in a data set pair</option>
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83 <option value="separate">paired - in two separate data sets</option>
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84 <option value="single">single</option>
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85 </param>
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86 <when value="paired">
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87 <param name="reads" argument="@ARGUMENT_FWD@/@ARGUMENT_REV@" type="data_collection" collection_type="@COLLECTION_TYPE@" format="fastq,fastq.gz" label="Paired short read data"/>
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88 </when>
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89 <when value="separate">
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90 <param name="reads" argument="@ARGUMENT_FWD@" type="data" format="fastq,fastq.gz" multiple="@MULTIPLE@" label="Forward read data"/>
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91 <param name="sdaer" argument="@ARGUMENT_REV@" type="data" format="fastq,fastq.gz" multiple="@MULTIPLE@" label="Reverse read data"/>
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92 </when>
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93 <when value="single">
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94 <param name="reads" argument="@ARGUMENT_FWD@" type="data" format="fastq,fastq.gz" multiple="@MULTIPLE@" label="Short read data"/>
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95 </when>
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96 </conditional>
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97 </xml>
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98
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99 <!-- for filterAndTrim -->
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100 <xml name="trimmers">
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101 <section name="trim" title="Trimming parameters" expanded="true">
0
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102 <param argument="truncQ" type="integer" value="2" min="0" label="Truncate reads at quality threshold" help="Truncate reads at the first instance of a quality score less than or equal to this threshold"/>
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103 <param argument="trimLeft" type="integer" value="0" min="0" label="Trim start of each read" help="The number of nucleotides to remove from the start of each read."/>
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104 <param argument="trimRight" type="integer" value="0" min="0" label="Trim end of each read" help="The number of nucleotides to remove from the end of each read"/>
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105 <param argument="truncLen" type="integer" value="0" min="0" label="Truncate read length" help="Truncate reads after this amount of bases. Reads shorter than this are discarded. (default 0: no truncation)"/>
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106 </section>
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107 </xml>
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108 <xml name="filters">
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109 <section name="filter" title="Filtering parameters" expanded="true">
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110 <param argument="maxLen" type="integer" value="" optional="true" min="0" label="Remove long reads" help="Remove reads with length greater than this value. Default: no length threshold"/>
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111 <param argument="minLen" type="integer" value="20" min="0" label="Remove short reads" help="Remove reads with length less than this value. Default: 20"/>
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112 <param argument="maxN" type="integer" value="0" min="0" label="Remove reads with more Ns" help="Note that some of the subsequent dada pipeline steps do not allow Ns"/>
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113 <param argument="minQ" type="integer" value="0" min="0" label="Remove low quality reads" help="Reads contain a quality score less than this value will be discarded"/>
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114 <param argument="maxEE" type="integer" value="" optional="true" min="0" label="Remove reads by number expected errors" help="Reads with a higher number of expected errors (EE) will be discarded, where EE = sum(10^(-Q_i/10)), with Q are the nominal quality scores at the read positions"/>
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115 </section>
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116 </xml>
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117
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118 <xml name="errorEstimationFunction">
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119 <param name="errfoo" argument="errorEstimationFunction" type="select" label="Error function">
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120 <option value="loessErrfun">loess: Use a loess fit to estimate error rates from transition counts</option>
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121 <option value="noqualErrfun">noqual: Estimate error rates for each type of transition while ignoring quality scores.</option>
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122 <option value="PacBioErrfun">PacBio: Estimate error rates from transition counts in PacBio CCS data.</option>
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123 </param>
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124 </xml>
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125 <token name="@HELP_OVERVIEW@"><![CDATA[
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126 Overview
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127 ........
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128
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129 The intended use of the dada2 tools for paired sequencing data is shown in the following image.
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130
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131 .. image:: pairpipe.png
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132
3
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133 Note: In particular for the analysis of paired collections the collections should be sorted lexicographical
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134 before the analysis.
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135
0
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136 For single end data you the steps "Unzip collection" and "mergePairs" are not necessary.
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137
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138 More information may be found on the dada2 homepage:: https://benjjneb.github.io/dada2/index.html (in particular tutorials) or the documentation of dada2's R package https://bioconductor.org/packages/release/bioc/html/dada2.html (in particular the pdf which contains the full documentation of all parameters)
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139 ]]></token>
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140 </macros>