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comparison dada2_removeBimeraDenovo.xml @ 11:efe26de231ec draft default tip

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit bbbc0e71b1db299a7c7296f25ac7adcccd27fbe3
author iuc
date Tue, 11 Feb 2025 23:28:29 +0000
parents 1770d188f72d
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10:1770d188f72d 11:efe26de231ec
1 <tool id="dada2_removeBimeraDenovo" name="dada2: removeBimeraDenovo" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09"> 1 <tool id="dada2_removeBimeraDenovo" name="dada2: removeBimeraDenovo" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
2 <description>Remove bimeras from collections of unique sequences</description> 2 <description>Remove bimeras from collections of unique sequences</description>
3 <macros> 3 <macros>
4 <import>macros.xml</import> 4 <import>macros.xml</import>
5 </macros> 5 </macros>
6 <expand macro="bio_tools"/> 6 <expand macro="bio_tools"/>
80 This tool can be used to remove chimeric sequences, i.e. sequences that can be constructed by combining a left-segment and a right-segment from two more abundant “parent” sequences.. Two methods to identify chimeras are supported: Identification from pooled sequences and identification by consensus across samples. 80 This tool can be used to remove chimeric sequences, i.e. sequences that can be constructed by combining a left-segment and a right-segment from two more abundant “parent” sequences.. Two methods to identify chimeras are supported: Identification from pooled sequences and identification by consensus across samples.
81 81
82 - from **pooled** sequences: Each sequence is evaluated against a set of "parents" drawn from the sequence collection that are sufficiently more abundant than the sequence being evaluated. Sequences that are bimera are removed, i.e. a two-parent chimera, in which the left side is made up of one parent sequence, and the right-side made up of a second parent sequence. 82 - from **pooled** sequences: Each sequence is evaluated against a set of "parents" drawn from the sequence collection that are sufficiently more abundant than the sequence being evaluated. Sequences that are bimera are removed, i.e. a two-parent chimera, in which the left side is made up of one parent sequence, and the right-side made up of a second parent sequence.
83 - by **consensus**: In short, bimeric sequences are flagged on a sample-by-sample basis. Then, a vote is performed for each sequence across all samples in which it appeared. If the sequence is flagged in a sufficiently high fraction of samples, it is identified as a bimera. A logical vector is returned, with an entry for each sequence in the table indicating whether it was identified as bimeric by this consensus procedure. 83 - by **consensus**: In short, bimeric sequences are flagged on a sample-by-sample basis. Then, a vote is performed for each sequence across all samples in which it appeared. If the sequence is flagged in a sufficiently high fraction of samples, it is identified as a bimera. A logical vector is returned, with an entry for each sequence in the table indicating whether it was identified as bimeric by this consensus procedure.
84 84
85 **Note**: pooled should only be used in combination with pooled denoising.
86
85 Usage 87 Usage
86 ..... 88 .....
87 89
88 **Input** 90 **Input**
89 91