Mercurial > repos > iuc > dada2_seqcounts
diff test-data.sh @ 2:f74c56549143 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit f2a33fe115fef9d711112b53136cf7619f1b19be"
author | iuc |
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date | Mon, 16 Mar 2020 08:13:07 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data.sh Mon Mar 16 08:13:07 2020 -0400 @@ -0,0 +1,58 @@ +#!/usr/bin/env bash + +# install conda +if type conda > /dev/null; then + true +else + tmp=$(mktemp -d) + wget https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh + bash Miniconda3-latest-Linux-x86_64.sh -b -p "$tmp/miniconda" + source "$tmp/miniconda/bin/activate" +fi + +eval "$(conda shell.bash hook)" + +# install conda env +if grep -Fq __bioconductor-dada2@1.14 <<< $(conda env list); then + true +else + conda create -y --quiet --override-channels --channel conda-forge --channel bioconda --channel defaults --name __bioconductor-dada2@1.14 bioconductor-dada2=1.14 +fi + +conda activate __bioconductor-dada2@1.14 + +# create test data +cd test-data/ + +# download Mothur SOP data from zenodo (GTN), same as +# http://www.mothur.org/w/images/d/d6/MiSeqSOPData.zip but stable links +# but file names need to be fixed +wget -nc -O F3D0_S188_L001_R1_001.fastq https://zenodo.org/record/800651/files/F3D0_R1.fastq?download=1 +wget -nc -O F3D0_S188_L001_R2_001.fastq https://zenodo.org/record/800651/files/F3D0_R2.fastq?download=1 +wget -nc -O F3D141_S207_L001_R1_001.fastq https://zenodo.org/record/800651/files/F3D141_R1.fastq?download=1 +wget -nc -O F3D141_S207_L001_R2_001.fastq https://zenodo.org/record/800651/files/F3D141_R2.fastq?download=1 + +# zip and reduce data to ~ 10% (for speed) +for i in *fastq +do + head -n 3000 "$i" | gzip -c > "$i.gz" +done +rm *fastq + +# download data bases from https://zenodo.org/record/158955 +# as mentioned in https://benjjneb.github.io/dada2/training.html +wget -nc -O reference.fa.gz https://zenodo.org/record/158955/files/rdp_train_set_14.fa.gz?download=1 +wget -nc -O reference_species.fa.gz https://zenodo.org/record/158955/files/rdp_species_assignment_14.fa.gz?download=1 + +# take ~ 5% of the reference (for speed) +zcat reference.fa.gz | head -n 1000 | gzip -c > t && mv t reference.fa.gz +zcat reference_species.fa.gz | head -n 1000 | gzip -c > t && mv t reference_species.fa.gz + + +# generate outputs +Rscript gentest.R + +conda deactivate + +# # remove files only needed for test generation +# rm learnErrors_F3D0_R2.pdf dada_F3D0_R2.Rdata