Mercurial > repos > iuc > data_manager_bowtie_index_builder

comparison data_manager/bowtie_index_builder.py @ 0:0a0c648498e2 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_bowtie_index_builder commit 418f745190d77983c3fb09badb6298493bccf4e0
author iuc
date Tue, 05 Jan 2016 12:57:11 -0500
parents
children 35c1e3785c90
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-1:000000000000 0:0a0c648498e2
1 #!/usr/bin/env python
2
3 import sys
4 import os
5 import tempfile
6 import optparse
7 import subprocess
8
9 from galaxy.util.json import from_json_string, to_json_string
10
11
12 CHUNK_SIZE = 2**20
13
14 DEFAULT_DATA_TABLE_NAME = "bowtie_indexes"
15
16 def get_id_name( params, dbkey, fasta_description=None):
17 #TODO: ensure sequence_id is unique and does not already appear in location file
18 sequence_id = params['param_dict']['sequence_id']
19 if not sequence_id:
20 sequence_id = dbkey
21
22 sequence_name = params['param_dict']['sequence_name']
23 if not sequence_name:
24 sequence_name = fasta_description
25 if not sequence_name:
26 sequence_name = dbkey
27 return sequence_id, sequence_name
28
29
30 def build_bowtie_index( data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME, color_space = False ):
31 #TODO: allow multiple FASTA input files
32 #tmp_dir = tempfile.mkdtemp( prefix='tmp-data-manager-bowtie-index-builder-' )
33 fasta_base_name = os.path.split( fasta_filename )[-1]
34 sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name )
35 os.symlink( fasta_filename, sym_linked_fasta_filename )
36 args = [ 'bowtie-build' ]
37 if color_space:
38 args.append( '-c' )
39 args.append( sym_linked_fasta_filename)
40 args.append( fasta_base_name )
41 args.append( sym_linked_fasta_filename )
42 tmp_stderr = tempfile.NamedTemporaryFile( prefix = "tmp-data-manager-bowtie-index-builder-stderr" )
43 proc = subprocess.Popen( args=args, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno() )
44 return_code = proc.wait()
45 if return_code:
46 tmp_stderr.flush()
47 tmp_stderr.seek(0)
48 print >> sys.stderr, "Error building index:"
49 while True:
50 chunk = tmp_stderr.read( CHUNK_SIZE )
51 if not chunk:
52 break
53 sys.stderr.write( chunk )
54 sys.exit( return_code )
55 tmp_stderr.close()
56 data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_name )
57 _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry )
58
59
60 def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ):
61 data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} )
62 data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] )
63 data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry )
64 return data_manager_dict
65
66
67 def main():
68 #Parse Command Line
69 parser = optparse.OptionParser()
70 parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' )
71 parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' )
72 parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' )
73 parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' )
74 parser.add_option( '-c', '--color_space', dest='color_space', action='store_true', default=False, help='color_space' )
75 (options, args) = parser.parse_args()
76
77 filename = args[0]
78
79 params = from_json_string( open( filename ).read() )
80 target_directory = params[ 'output_data' ][0]['extra_files_path']
81 os.mkdir( target_directory )
82 data_manager_dict = {}
83
84 dbkey = options.fasta_dbkey
85
86 if dbkey in [ None, '', '?' ]:
87 raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) )
88
89 sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description )
90
91 #build the index
92 build_bowtie_index( data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME, color_space=options.color_space )
93
94 #save info to json file
95 open( filename, 'wb' ).write( to_json_string( data_manager_dict ) )
96
97 if __name__ == "__main__":
98 main()