Mercurial > repos > iuc > data_manager_bowtie_index_builder
view data_manager/bowtie_index_builder.py @ 5:08a3583826fa draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_bowtie_index_builder commit 02d2967f77e3fa5a18aea63dc84aa9ab418dc165"
author | iuc |
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date | Sun, 22 Nov 2020 12:44:59 +0000 |
parents | 86e9af693a33 |
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#!/usr/bin/env python from __future__ import print_function import json import optparse import os import subprocess import sys import tempfile CHUNK_SIZE = 2**20 DEFAULT_DATA_TABLE_NAME = "bowtie_indexes" def get_id_name(params, dbkey, fasta_description=None): # TODO: ensure sequence_id is unique and does not already appear in location file sequence_id = params['param_dict']['sequence_id'] if not sequence_id: sequence_id = dbkey sequence_name = params['param_dict']['sequence_name'] if not sequence_name: sequence_name = fasta_description if not sequence_name: sequence_name = dbkey return sequence_id, sequence_name def build_bowtie_index(data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME, color_space=False): # TODO: allow multiple FASTA input files fasta_base_name = os.path.split(fasta_filename)[-1] sym_linked_fasta_filename = os.path.join(target_directory, fasta_base_name) os.symlink(fasta_filename, sym_linked_fasta_filename) args = ['bowtie-build'] if color_space: args.append('-C') args.append(sym_linked_fasta_filename) args.append(fasta_base_name) args.append(sym_linked_fasta_filename) tmp_stderr = tempfile.NamedTemporaryFile(prefix="tmp-data-manager-bowtie-index-builder-stderr") proc = subprocess.Popen(args=args, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno()) return_code = proc.wait() if return_code: tmp_stderr.flush() tmp_stderr.seek(0) print("Error building index:", file=sys.stderr) while True: chunk = tmp_stderr.read(CHUNK_SIZE) if not chunk: break sys.stderr.write(chunk) sys.exit(return_code) tmp_stderr.close() data_table_entry = dict(value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_name) _add_data_table_entry(data_manager_dict, data_table_name, data_table_entry) def _add_data_table_entry(data_manager_dict, data_table_name, data_table_entry): data_manager_dict['data_tables'] = data_manager_dict.get('data_tables', {}) data_manager_dict['data_tables'][data_table_name] = data_manager_dict['data_tables'].get(data_table_name, []) data_manager_dict['data_tables'][data_table_name].append(data_table_entry) return data_manager_dict def main(): parser = optparse.OptionParser() parser.add_option('-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename') parser.add_option('-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey') parser.add_option('-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description') parser.add_option('-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name') parser.add_option('-c', '--color_space', dest='color_space', action='store_true', default=False, help='color_space') (options, args) = parser.parse_args() filename = args[0] with open(filename) as fh: params = json.load(fh) target_directory = params['output_data'][0]['extra_files_path'] os.mkdir(target_directory) data_manager_dict = {} dbkey = options.fasta_dbkey if dbkey in [None, '', '?']: raise Exception('"%s" is not a valid dbkey. You must specify a valid dbkey.' % (dbkey)) sequence_id, sequence_name = get_id_name(params, dbkey=dbkey, fasta_description=options.fasta_description) # build the index build_bowtie_index(data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME, color_space=options.color_space) # save info to json file with open(filename, 'w') as fh: json.dump(data_manager_dict, fh, sort_keys=True) if __name__ == "__main__": main()