Mercurial > repos > iuc > data_manager_star_index_builder
comparison data_manager/macros.xml @ 7:6c6c6df09e64 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_star_index_builder commit a8e319862d723654c372a6d71e5de76e052586a9"
| author | iuc |
|---|---|
| date | Wed, 05 Aug 2020 09:38:12 -0400 |
| parents | 64deddb6a8ec |
| children | c520a52b5174 |
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| 6:64deddb6a8ec | 7:6c6c6df09e64 |
|---|---|
| 3 whenever you make changes to the following two version tokens! | 3 whenever you make changes to the following two version tokens! |
| 4 The data manager uses a symlink to this macro file to keep the STAR and | 4 The data manager uses a symlink to this macro file to keep the STAR and |
| 5 the index versions in sync, but you should manually adjust the +galaxy | 5 the index versions in sync, but you should manually adjust the +galaxy |
| 6 version number. --> | 6 version number. --> |
| 7 <!-- STAR version to be used --> | 7 <!-- STAR version to be used --> |
| 8 <token name="@VERSION@">2.7.2b</token> | 8 <token name="@VERSION@">2.7.5b</token> |
| 9 <!-- STAR index version compatible with this version of STAR | 9 <!-- STAR index version compatible with this version of STAR |
| 10 This is the STAR version that introduced the index structure expected | 10 This is the STAR version that introduced the index structure expected |
| 11 by the current version. | 11 by the current version. |
| 12 It can be found for any specific version of STAR with: | 12 It can be found for any specific version of STAR with: |
| 13 STAR -h | grep versionGenome | 13 STAR -h | grep versionGenome |
| 14 or by looking for the versionGenome parameter in source/parametersDefault | 14 or by looking for the versionGenome parameter in source/parametersDefault |
| 15 of STAR's source code --> | 15 of STAR's source code --> |
| 16 <token name="@IDX_VERSION@">2.7.1a</token> | 16 <token name="@IDX_VERSION@">2.7.4a</token> |
| 17 <token name="@IDX_DATA_TABLE@">rnastar_index2x_versioned</token> | |
| 17 | 18 |
| 18 <xml name="requirements"> | 19 <xml name="requirements"> |
| 19 <requirements> | 20 <requirements> |
| 20 <requirement type="package" version="@VERSION@">star</requirement> | 21 <requirement type="package" version="@VERSION@">star</requirement> |
| 21 <requirement type="package" version="1.9">samtools</requirement> | 22 <requirement type="package" version="1.9">samtools</requirement> |
| 25 | 26 |
| 26 <xml name="index_selection" token_with_gene_model="0"> | 27 <xml name="index_selection" token_with_gene_model="0"> |
| 27 <param argument="--genomeDir" name="genomeDir" type="select" | 28 <param argument="--genomeDir" name="genomeDir" type="select" |
| 28 label="Select reference genome" | 29 label="Select reference genome" |
| 29 help="If your genome of interest is not listed, contact the Galaxy team"> | 30 help="If your genome of interest is not listed, contact the Galaxy team"> |
| 30 <options from_data_table="rnastar_index2_versioned"> | 31 <options from_data_table="@IDX_DATA_TABLE@"> |
| 31 <filter type="static_value" column="4" value="@WITH_GENE_MODEL@" /> | 32 <filter type="static_value" column="4" value="@WITH_GENE_MODEL@" /> |
| 32 <filter type="static_value" column="5" value="@IDX_VERSION@" /> | 33 <filter type="static_value" column="5" value="@IDX_VERSION@" /> |
| 33 <filter type="sort_by" column="2" /> | 34 <filter type="sort_by" column="2" /> |
| 34 <validator type="no_options" message="No indexes are available for the selected input dataset" /> | 35 <validator type="no_options" message="No indexes are available for the selected input dataset" /> |
| 35 </options> | 36 </options> |
| 51 <xml name="dbKeyActions"> | 52 <xml name="dbKeyActions"> |
| 52 <actions> | 53 <actions> |
| 53 <conditional name="refGenomeSource.geneSource"> | 54 <conditional name="refGenomeSource.geneSource"> |
| 54 <when value="indexed"> | 55 <when value="indexed"> |
| 55 <action type="metadata" name="dbkey"> | 56 <action type="metadata" name="dbkey"> |
| 56 <option type="from_data_table" name="rnastar_index2_versioned" column="1" offset="0"> | 57 <option type="from_data_table" name="@IDX_DATA_TABLE@" column="1" offset="0"> |
| 57 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> | 58 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> |
| 58 <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/> | 59 <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/> |
| 59 </option> | 60 </option> |
| 60 </action> | 61 </action> |
| 61 </when> | 62 </when> |
| 74 STAR | 75 STAR |
| 75 --runMode genomeGenerate | 76 --runMode genomeGenerate |
| 76 --genomeDir 'tempstargenomedir' | 77 --genomeDir 'tempstargenomedir' |
| 77 --genomeFastaFiles '${refGenomeSource.genomeFastaFiles}' | 78 --genomeFastaFiles '${refGenomeSource.genomeFastaFiles}' |
| 78 ## Handle difference between indices with/without annotations | 79 ## Handle difference between indices with/without annotations |
| 79 #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf': | 80 #if 'GTFconditional' in $refGenomeSource: |
| 80 --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}' | 81 ## GTFconditional exists only in STAR, but not STARsolo |
| 81 --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}' | 82 #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf': |
| 82 #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3': | 83 --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}' |
| 84 --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}' | |
| 85 #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3': | |
| 86 --sjdbGTFtagExonParentTranscript Parent | |
| 87 #end if | |
| 88 #end if | |
| 89 #else: | |
| 90 ## ref genome selection is less complex for STARsolo cause | |
| 91 ## with-gtf is mandatory there | |
| 92 --sjdbOverhang '${refGenomeSource.sjdbOverhang}' | |
| 93 --sjdbGTFfile '${refGenomeSource.sjdbGTFfile}' | |
| 94 #if str($refGenomeSource.sjdbGTFfile.ext) == 'gff3': | |
| 83 --sjdbGTFtagExonParentTranscript Parent | 95 --sjdbGTFtagExonParentTranscript Parent |
| 84 #end if | 96 #end if |
| 85 #end if | 97 #end if |
| 86 #if str($refGenomeSource.genomeSAindexNbases): | 98 #if str($refGenomeSource.genomeSAindexNbases): |
| 87 --genomeSAindexNbases ${refGenomeSource.genomeSAindexNbases} | 99 --genomeSAindexNbases ${refGenomeSource.genomeSAindexNbases} |
| 108 #end if | 120 #end if |
| 109 #end if | 121 #end if |
| 110 #end if | 122 #end if |
| 111 #end if | 123 #end if |
| 112 ]]></token> | 124 ]]></token> |
| 125 <xml name="ref_selection"> | |
| 126 <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" /> | |
| 127 <!-- Currently, this parameter is not exposed in the wrapper, | |
| 128 but used only in the tests to avoid excessive index sizes for | |
| 129 the tiny test genomes. --> | |
| 130 <param name="genomeSAindexNbases" type="hidden" value="" /> | |
| 131 </xml> | |
| 113 <xml name="stdio" > | 132 <xml name="stdio" > |
| 114 <stdio> | 133 <stdio> |
| 115 <regex match="FATAL error" source="both" level="fatal"/> | 134 <regex match="FATAL error" source="both" level="fatal"/> |
| 116 <regex match="EXITING: FATAL INPUT ERROR:" source="both" level="fatal"/> | 135 <regex match="EXITING: FATAL INPUT ERROR:" source="both" level="fatal"/> |
| 117 <regex match="EXITING: fatal error trying to allocate genome arrays, exception thrown: std::bad_alloc" source="both" level="fatal"/> | 136 <regex match="EXITING: fatal error trying to allocate genome arrays, exception thrown: std::bad_alloc" source="both" level="fatal"/> |
| 118 <regex match="\[sam_read1\] missing header\? Abort!" source="both" level="fatal"/> | 137 <regex match="\[sam_read1\] missing header\? Abort!" source="both" level="fatal"/> |
| 119 <yield /> | 138 <yield /> |
| 120 </stdio> | 139 </stdio> |
| 121 </xml> | 140 </xml> |
| 122 <xml name="refgenomehandling" > | |
| 123 <conditional name="refGenomeSource"> | |
| 124 <param name="geneSource" type="select" label="Custom or built-in reference genome" help="Built-ins were indexed using default options"> | |
| 125 <option value="indexed" selected="true">Use a built-in index</option> | |
| 126 <option value="history">Use reference genome from history and create temporary index</option> | |
| 127 </param> | |
| 128 <when value="indexed"> | |
| 129 <conditional name="GTFconditional"> | |
| 130 <param name="GTFselect" type="select" | |
| 131 label="Reference genome with or without an annotation" | |
| 132 help="Select the '... with builtin gene-model' option to select from the list of available indexes that were built with splice junction information. Select the '... without builtin gene-model' option to select from the list of available indexes without annotated splice junctions."> | |
| 133 <option value="without-gtf" selected='true'>use genome reference without builtin gene-model</option> | |
| 134 <option value="with-gtf">use genome reference with builtin gene-model</option> | |
| 135 </param> | |
| 136 <when value="with-gtf"> | |
| 137 <expand macro="index_selection" with_gene_model="1" /> | |
| 138 </when> | |
| 139 <when value="without-gtf"> | |
| 140 <expand macro="index_selection" with_gene_model="0" /> | |
| 141 <expand macro="@SJDBOPTIONS@" /> | |
| 142 </when> | |
| 143 </conditional> | |
| 144 </when> | |
| 145 <when value="history"> | |
| 146 <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" /> | |
| 147 <!-- Currently, this parameter is not exposed in the wrapper, | |
| 148 but used only in the tests to avoid excessive index sizes for | |
| 149 the tiny test genomes. --> | |
| 150 <param name="genomeSAindexNbases" type="hidden" value="" /> | |
| 151 <conditional name="GTFconditional"> | |
| 152 <param name="GTFselect" type="select" | |
| 153 label="Build index with our without known splice junctions annotation" | |
| 154 help="To build an index with known splice junctions annotated, you will have to provide a GTF or GFF3 dataset that describes the gene models (the location of genes, transcripts and exons) known for the reference genome."> | |
| 155 <option value="without-gtf">build index without gene-model</option> | |
| 156 <option value="with-gtf">build index with gene-model</option> | |
| 157 </param> | |
| 158 <when value="with-gtf"> | |
| 159 <expand macro="@SJDBOPTIONS@" optional="false"/> | |
| 160 </when> | |
| 161 <when value="without-gtf" /> | |
| 162 </conditional> | |
| 163 </when> | |
| 164 </conditional> | |
| 165 </xml> | |
| 166 </macros> | 141 </macros> |
